| | The GraphPad Guide to Analyzing Radioligand Binding Data (Site not responding. Last check: 2007-10-19) |
 | | Bmax is the total number of receptors expressed in the same units as the Y values (i.e., cpm, sites/cell or fmol/mg protein) and Kd is the equilibrium dissociation constant (expressed in the same units as [L], usually nM). |
 | | It is the equilibrium dissociation constant for binding of the unlabeled drug - the concentration of the unlabeled drug that will bind to half the binding sites at equilibrium in the absence of radioligand or other competitors. |
 | | If the dissociation rate constant is any faster, it would be difficult to perform radioligand binding experiments as the radioligand would dissociate from the receptors while you wash the filters. |
| www.graphpad.com /www/radiolig/radiolig1.htm (6758 words) |