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Topic: Dithiothreitol

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	Minotech biotechnology Products
		(pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol.
		50 mM NaCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol.
		50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol.
	www.minotech.gr /html/Endonucl6.htm (518 words)

	Minotech biotechnology Products
		100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol.
		7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, and 50% glycerol.
		200 mM NaCl, 20 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol.
	www.imbb.forth.gr /groups/minotech/html/products.htm (492 words)

	[No title]
		The addition of sodium metabisulfite to a Eagle's minimal essential medium with rabbit serum was found to have an effect similar to that of dithiothreitol in the survival of T. pallidum under 3% O2.
		E0210 The effects of the concentrations of dithiothreitol, molecular oxygen, and antioxidants on the in vitro replication of T. pallidum were studied [1].
		Retinol (vitamin A) The effects of the concentrations of dithiothreitol, molecular oxygen, and antioxidants on the in vitro replication of T. pallidum were studied [1].
	igs-server.cnrs-mrs.fr /axenic-cgi/generate_table?Treponema+no+on+off (4297 words)

	MucoGest - for TB and Mycology Specimen Digestion
		is composed of dithiothreitol, a mucolytic agent recommended for use in the digestion and decontamination procedures of sputum and other clinical specimens for the recovery of mycobacteria and fungi.
		Dithiothreitol serves as a mucolytic agent by disrupting disulfide bonds in the mucus of sputum.
		Reep and Kaplan also determined dithiothreitol to be useful in liquefying sputa for mycological culture.
	www.hardydiagnostics.com /catalog/hugo/MucoGest.htm (1255 words)

	Minotech biotechnology Products
		(pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 400 μg/ml BSA and 50% glycerol.
		50 mM KCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol.
		(pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 500 μg/ml BSA and 50% glycerol.
	www.minotech.gr /html/Endonucl7.htm (601 words)

	Probing ligand-induced conformational changes of human CD38 -- Berthelier et al. 267 (10): 3056 -- FEBS Journal (Site not responding. Last check: 2007-10-15)
		dithiothreitol during 30 min at 37 °C in buffer A, in the absence or in the presence of 2 m
		(A) CD38 staining of the cells treated 45 min in the presence of dithiothreitol, in the absence (light trace) or in the presence (heavy trace) of the indicated compounds.
		5. Kinetics of inactivation by dithiothreitol of the NAD
	content.febsjournal.org /cgi/content/full/267/10/3056 (4883 words)

	Jena Bioscience - Products & Ordering - Enzymes (Cloning Enzymes such as Restriction Enzymes, Polymerases, ...) - ...
		Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 500 µg/ml BSA and 50 % glycerol.
		Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol.
		Storage buffer: 300 mM KCl, 10 mM Tris-HCl (pH 7.5 @ 25°C), 1 mM EDTA, 1 mM dithiothreitol, 100 µg/ml gelatin and 50 % glycerol.
	jenabioscience.com /index.php/.../1/catalog/410 (481 words)

	Co-existence of two regulatory NADP-glyceraldehyde 3-P dehydrogenase complexes in higher plant chloroplasts -- Scheibe ... (Site not responding. Last check: 2007-10-15)
		10 mm dithiothreitol, pH 6.9, the PRK was eluted in 10 mm potassium
		phosphate, pH 7.2, 10 mm dithiothreitol, and 5 mm ATP.
		Preincubation of spinach chloroplasts with 20 mm dithiothreitol
	content.febsjournal.org /cgi/content/full/269/22/5617 (4265 words)

	ePrintsUQ - Effect Of Exogenous Cholesterol And Dithiothreitol On The Activity Of Human Liver Microsomal Acyl-Coenzyme ...
		Smith, Jeffery L. and Madden, Lesley J. and de Jersey, John (1996) Effect Of Exogenous Cholesterol And Dithiothreitol On The Activity Of Human Liver Microsomal Acyl-Coenzyme A:Cholesterol Acyltransferase (ACAT).
		The effects of exogenous cholesterol and dithiothreitol (DTT) on the ACAT activity of human liver microsomes have been determined.
		Originally published as Jeffery L. Smith, Lesley J. Madden and John de Jersey, "Effect Of Exogenous Cholesterol And Dithiothreitol On The Activity Of Human Liver Microsomal Acyl-Coenzyme A:Cholesterol Acyltransferase (ACAT)", Clinica Chimica Acta, 256 (1), 1996, pp.
	eprint.uq.edu.au /archive/00001357 (377 words)

	Integrin Activation by Dithiothreitol or Mn2+ Induces a Ligand-occupied Conformation and Exposure of a Novel ...
		Integrin Activation by Dithiothreitol or Mn2+ Induces a Ligand-occupied Conformation and Exposure of a Novel NH2-terminal Regulatory Site on the beta 1 Integrin Chain -- Ni et al.
		Integrin Activation by Dithiothreitol or Mn Induces a Ligand-occupied Conformation and Exposure of a Novel NH
		Dithiothreitol or Mn activation of integrin-mediated adhesion in the human B cell
	www.jbc.org /cgi/content/abstract/273/14/7981 (691 words)

	Combination of Dithiothreitol and Detergent Treatment of Spermatozoa Causes Paternal Chromosomal Damage -- Szczygiel ...
		dithiothreitol (DTT) in the presence of the nonionic detergent
		Rao B, David G. Improved recovery of post-thaw motility and vitality of human spermatozoa cryopreserved in the presence of dithiothreitol.
		Tateno H, Kamiguchi Y. Dithiothreitol induces sperm nuclear decondensation and protects against chromosome damage during male pronuclear formation in hybrid zygotes between Chinese hamster spermatozoa and Syrian hamster oocytes.
	www.biolreprod.org /cgi/content/full/67/5/1532 (3941 words)

	DITHIOTHREITOL - BioForum
		Additive effects of bovine serum albumin, dithiothreitol, and glycerol on PCR.
		DTT (dithiothreitol) is used to reduce disulfide bonds quantitatively and maintain monothiols in the reduced state.
		At low concentrations, DTT stabilizes enzymes and other proteins which possess free sulfhydryl groups and has been shown to restore activity lost by oxidation of these groups in vitro.
	www.protocol-online.org /forums/index.php?showtopic=3312 (245 words)

	Effect of sputum processing with dithiothreitol on the detection of inflammatory mediators in chronic bronchitis and ...
		Effect of sputum processing with dithiothreitol on the detection of inflammatory mediators in chronic bronchitis and bronchiectasis -- Woolhouse et al.
		Effect of sputum processing with dithiothreitol on the detection of inflammatory mediators in chronic bronchitis and bronchiectasis
		Induced sputum cell and fluid-phase indices of inflammation: comparison of treatment with dithiothreitol vs phosphate-buffered saline.
	thorax.bmjjournals.com /cgi/content/full/57/8/667 (2580 words)

	Dithiothreitol Enhances Arsenic Trioxide-Induced Apoptosis in NB4 Cells -- Gurr et al. 56 (1): 102 -- Molecular ...
		Dithiothreitol Enhances Arsenic Trioxide-Induced Apoptosis in NB4 Cells -- Gurr et al.
		APL, acute promyelocytic leukemia; DTT, dithiothreitol; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; pNA, p-nitroanilide.
		Cruse WBT and James MNG (1972) The crystal structure of the arsenite complex of dithiothreitol.
	molpharm.aspetjournals.org /cgi/content/full/56/1/102 (4977 words)

	Method for the S-Carboxymethylation of Cysteine
		Add 10 µL 1M dithiothreitol and let the reduction proceed for 4hrs ambient or for a shorter time at 37°C, (60 min.).
		Add 20 µL 1M iodoacetic acid, mix and let stand for 30 min ambient in the dark.
		Add 40 µL 1M dithiothreitol to quench the iodoacetic acid.
	www.ionsource.com /Card/cmc/method.htm (156 words)

	Effect of Ceramide on KCa Channel Activity and Vascular Tone in Coronary Arteries -- Li et al. 33 (6): 1441 -- ... (Site not responding. Last check: 2007-10-15)
		In contrast to the marked effect of dithiothreitol in cell-attached
		Effect of dithiothreitol (DTT) (A) and glutathione (GSH) (B) on acidic SMase and neutral SMase activity in coronary arterial homogenate.
		is considerable evidence indicating that the effect of dithiothreitol
	hyper.ahajournals.org /cgi/content/full/33/6/1441 (2816 words)

	ACTA Jul2003: Thin-Layer Preparations of Dithiothreitol-Treated Bronchial Washing Specimens (Site not responding. Last check: 2007-10-15)
		Authors: Kirsti I. Koivurinne, M.App.Sci., C.T.(ASC), and Paul W. Shield, Ph.D. Objective: To evaluate the combined effect of dithiothreitol (DTT) treatment and ThinPrep (TP) (Cytyc Corp, Boxborough, Massachusetts, U.S.A.) processing on bronchial washing specimens.
		Study Design: A total of 431 bronchial washing specimens were initially treated with 0.05% DTT in a 30% methanol solution.
		Conclusion: Improvement in specimen quality and reduced screening time have to be balanced against the high cost of consumables with the TP technique.
	www.acta-cytol.com /Abs/ACTA1359.htm (247 words)

	biology - SDS-PAGE
		A tracking dye may be added to the protein solution to allow the experimentor to track the progress of the protein solution through the gel during the electrophoretic run.
		Besides the addition of SDS, proteins may optionally be boiled in the presence of a reducing agent, such as dithiothreitol (DTT) or 2-mercaptoethanol, which further denatures the proteins by reducing disulfide linkages, thus overcoming some forms of tertiary protein folding, and breaking up quaternary protein structure (oligomeric subunits).
		This is known as reducing SDS-PAGE, and is most commonly used.
	www.biologydaily.com /biology/SDS-PAGE (640 words)

	Calbiochem: CB0121: Cleland's Reagent (Dithiothreitol) Technical Bulletin
		•Cleland's Reagent (Dithiothreitol) Technical Bulletin PDF (125 KB)
		Discusses the uses of DTT, a laboratory staple, as well as two newer fast-acting reducing agents - TCEP and BMS.
		Download Cleland's Reagent (Dithiothreitol) Technical Bulletin PDF (125 KB)
	www.emdbiosciences.com /product/CB0121 (90 words)

	Energy Citations Database (ECD) - Energy and Energy-Related Bibliographic Citations
		Energy Citations Database (ECD) Document #4141665 - RADIOPROTECTION AND RECOVERY BY DITHIOTHREITOL.
		Availability information may be found in the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or via the "Full-text Availability" link.
		THREITOL, 1,4-DITHIO-/radioprotective effects of oxidized and reduced form of, in mice, x;X RADIATION/effects on mice, protective effects of oxidized and reduced dithiothreitol against;MICE/radiation effects on, protective effects of oxidized and reduced dithiothreitol against x
	www.osti.gov /energycitations/product.biblio.jsp?osti_id=4141665 (116 words)

	Increased Concentrations of Radioisotopically-labeled Complementary Ribonucleic Acid Probe, Dextran Sulfate, and ...
		Increased Concentrations of Radioisotopically-labeled Complementary Ribonucleic Acid Probe, Dextran Sulfate, and Dithiothreitol in the Hybridization Buffer Can Improve Results of In Situ Hybridization Histochemistry -- Hrabovszky and Petersen 50 (10): 1389 -- Journal of Histochemistry and Cytochemistry
		Increased Concentrations of Radioisotopically-labeled Complementary Ribonucleic Acid Probe, Dextran Sulfate, and Dithiothreitol in the Hybridization Buffer Can Improve Results of In Situ Hybridization Histochemistry
		probes, dextran sulfate (DS), and dithiothreitol (DTT) in the
	www.jhc.org /cgi/content/full/50/10/1389 (5706 words)

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