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Topic: DnaB

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	dnaC -
		Replication of bacteriophage phiK duplex replicative-form DNA in dnaB and dnaC mutants of Escherichia coli.
		The ratio of the dnaC and dnaB polypeptides in the complex is approximately 1, as determined on a denaturing gel.
		Kinetic analysis of the ATPase activity of dnaB X dnaC complex indicates that the decrease in the ATPase activity on complex formation is due to a reduction of the maximal velocity (Vmax).
	www.ihop-net.org /UniPub/iHOP/gg/171931.html (4211 words)

	ms+biblio (Site not responding. Last check: 2007-10-20)
		DnaB is involved in both the initiation and the elongation stages of DNA replication, and it plays a key role in the replication of bacterial chromosomal, phage, and plasmid DNA (Kornberg and Baker, 1991).
		DnaB protein was separated from DnaC and the DnaB.DnaC complex by chromatography on a column of DEAE-cellulose (Kobori and Kornberg, 1982), and further purified by gel filtration through Sephacryl S-400 (Pharmacia) in Buffer A (50 mM Tris.HCl, pH 7.6, 2 mM dithiothreitol, 5 mM MgCl2, 0.1 mM ATP, 200 mM NaCl, 20% (v/v) glycerol).
		The planes corresponding to the reconstructed DnaB oligomer are presented in Figure 5, and Figure 6a and Figure 6b show two orientations of a surface rendering of the volume.
	www.cnb.uam.es /~bioinfo/paper/carmen/carmen.html (5094 words)

	Illegitimate Recombination Induced by Overproduction of DnaB Helicase in Escherichia coli -- Yamashita et al. 181 (15): ...
		Illegitimate Recombination Induced by Overproduction of DnaB Helicase in Escherichia coli
		Inhibition of DnaB helicase by a dnaB(Ts) mutation
		The DnaB protein is a DNA helicase essential for DNA replication of bacterial, as well as phage, chromosomes (13).
	jb.asm.org /cgi/content/full/181/15/4549 (2344 words)

	Bacteriophage P1 Ban protein is a hexameric DNA helicase that interacts with and substitutes for Escherichia coli DnaB ...
		The asterisk on the dnaB interrupted gene symbolises the dnaB368 amber mutation.
		Saluja,D. and Godson,G.N. (1995) Biochemical characterization of Escherichia coli temperature-sensitive dnaB mutants dnaB8, dnaB252, dnaB70, dnaB43, and dnaB454.
		Yamashita,T., Hanada,K., Iwasaki,M., Yamaguchi,H. and Ikeda,H. (1999) Illegitimate recombination induced by overproduction of DnaB helicase in Escherichia coli.
	nar.oxfordjournals.org /cgi/content/full/31/14/3918 (6808 words)

	The DnaC helicase loader is a dual ATP/ADP switch protein
		The DnaB mutant is inactive as a helicase, and thus the observed bubble expansion is not due to helicase action.
		Normally, DnaC is not stuck tightly to DnaB loaded onto DNA and readily dissociates during gel filtration, freeing DnaB for helicase action (Kobori and Kornberg, 1982; Funnell et al., 1987; Fang et al., 1999).
		DNA unwinding by DnaB was measured by displacement of a 30mer DNA oligonucleotide annealed to M13mp18 as modified from Shrimankar et al.
	www.nature.com /emboj/journal/v21/n12/full/7594533a.html (10135 words)

	Expression of the dnaB gene of Escherichia coli is inducible by replication-blocking DNA damage in a recA-independent ...
		Expression of the dnaB gene of Escherichia coli is inducible by replication-blocking DNA damage in a recA-independent manner.
		Expression of the dnaB gene of Escherichia coli is inducible by replication-blocking DNA damage in a recA-independent manner.The replicative DNA helicase encoded by the dnaB gene is essential for chromosomal DNA replication in Escherichia coli.
		The DnaB protein is a component of the phi X-type primosome which is regarded as a model system for lagging strand synthesis of the chromosome.
	www.pdg.cnb.uam.es /UniPub/iHOP/gp/416389.html (254 words)

	Defective replication activity of a dominant-lethal dnaB gene product from Escherichia coli. (Site not responding. Last check: 2007-10-20)
		dnaB protein of Escherichia coli is an essential replication protein.
		By contrast, it was not inhibitory to dnaB protein in priming of single-stranded DNA by primase in the absence of single-stranded DNA binding protein.
		Biochemical analysis of the altered form of dnaB protein revealed that it was inactive in replication in several purified enzyme systems which involve specific and nonspecific primer formation on single-stranded DNAs, and in replication of plasmids containing the E. coli chromosomal origin.
	www.pdg.cnb.uam.es /UniPub/iHOP/gp/70428.html (202 words)

	The DnaB{middle dot}DnaC complex: a structure based on dimers assembled around an occluded channel -- Bárcena et ... (Site not responding. Last check: 2007-10-20)
		DnaB is the major replicative helicase in Escherichia coli.
		The domain organization of the DnaB monomer is summarized in the scheme (inset), where numbers refer to residue positions in the primary structure.
		In this case, in the surface rendering that accounts for 40% of the mass, the region considered to be DnaB is highlighted in pink.
	embojournal.npgjournals.com /cgi/content/full/20/6/1462 (4562 words)

	Cryptic single-stranded-DNA binding activities of the phage lambda P and Escherichia coli DnaC replication initiation ...
		DnaB helicase (16) and the helicase is recruited to the viral
		DnaB apparently is stabilized by the presence of AMP-PNP in the
		The failure to detect the transfer of DnaB onto the oligonucleotide in the presence of the chaperones and ATP was predictable.
	www.pnas.org /cgi/content/full/94/4/1154 (5005 words)

	Interactive Fly, Drosophila
		dnab RNA is expressed exclusively in a subset of neuroblasts in the embryonic and larval central nervous system (CNS), as well as in several larval imaginal disc tissues.
		dnab expression was examined in cas minus embryos, which contain deletions removing the entire castor gene; dnab expression is affected in these mutants.
		Alternatively, dnab expression might be directly regulated by the transcription factors encoded by the Castor target genes drifter, Acj6, pdm-1, or pdm-2.
	www.sdbonline.org /fly/neural/ming3.htm (1160 words)

	The Bacterial Replicative Helicase DnaB Evolved from a RecA Duplication -- Leipe et al. 10 (1): 5 -- Genome Research
		The DnaB helicase is a crucial protein in bacterial DNA replication.
		The central parallel µ-sheet is blue and the elements that are involved in coordinating loop 1 (between strand 4 and helix F) and loop 2 (between strand 5 and helix G) are green.
		NMR structure of the N-terminal domain of E. coli DnaB helicase: Implications for structure rearrangements in the helicase hexamer.
	www.genome.org /cgi/content/full/10/1/5 (6191 words)

	New intein in Cyanobacteria (Site not responding. Last check: 2007-10-20)
		The two DnaB inteins could be the result of a single integration event into a common ancestor of the cyanobacterial and chloroplast dnaB genes.
		Selection on the DnaB helicases is probably linked to changes in the proteins with which they interact and to the evolution of the whole DNA replication process.
		The two DnaB proteins can be aligned end to end (415 amino acids) with only 3 gaps, 37% amino acid identities and 79% amino acid identities and conserved substitutions.
	bioinformatics.weizmann.ac.il /~pietro/inteins/Ssp_dnaB.html (1153 words)

	A DnaB intein in Rhodothermus marinus: Indication of recent intein homing across remotely related organisms -- Liu and ...
		A dnaB gene encoding a homologue of the Escherichia coli DNA helicase DnaB was cloned and sequenced in the thermophilic eubacterium
		DnaB intein coding sequence may be of foreign origin.
		marinus dnaB gene was clearly identified by the protein sequence it encodes, despite the presence of an intein.
	www.pnas.org /cgi/content/full/94/15/7851 (3213 words)

	Identification and Characterization of a Novel Allele of Escherichia coli dnaB Helicase That Compromises the Stability ...
		Identification and Characterization of a Novel Allele of Escherichia coli dnaB Helicase That Compromises the Stability of Plasmid P1 -- Slavcev and Funnell 187 (4): 1227 -- The Journal of Bacteriology
		Nucleotide sequence of dnaB and the primary structure of the protein from Escherichia coli.
		Biochemical characterization of Escherichia coli temperature-sensitive dnaB mutants dnaB8, dnaB252, dnaB70, dnaB43, and dnaB454.
	jb.asm.org /cgi/content/full/187/4/1227 (6136 words)

	OhioLINK ETD: Shrimakar, Paresh
		The dnaB gene of E.coli encodes an essential DNA replication enzyme.
		In addition, general priming reactions with wild-type DnaB or RC231 in combination with ATP or ATPγS exhibited striking differences in both the efficiency of priming and the length distribution of pr imers.
		Analysis of these effects suggests that primase interacts directly with DnaB throughout primer synthesis until ATP hydrolysis by DnaB disrupts the interaction and leads to primer termination.
	www.ohiolink.edu /etd/view.cgi?case1059665664 (363 words)

	Pfam 18.0 : DnaB (Site not responding. Last check: 2007-10-20)
		The hexameric helicase DnaB unwinds the DNA duplex at the Escherichia coli chromosome replication fork.
		Although the mechanism by which DnaB both couples ATP hydrolysis to translocation along DNA and denatures the duplex is unknown, a change in the quaternary structure of the protein involving dimerisation of the N-terminal domain has been observed and may occur during the enzymatic cycle.
		This N-terminal domain is required both for interaction with other proteins in the primosome and for DnaB helicase activity [1].
	pfam.wustl.edu /cgi-bin/getdesc?name=DnaB (186 words)

	The Interaction of Bacteriophage P2 B Protein with Escherichia coli DnaB Helicase -- Odegrip et al. 74 (9): 4057 -- The ...
		P2 B protein were shown to coprecipitate the DnaB protein.
		a reaction that is facilitated by the inactivation of DnaB.
		Zhong, Z., Helinski, D., Toukdarian, A. A Specific Region in the N Terminus of a Replication Initiation Protein of Plasmid RK2 Is Required for Recruitment of Pseudomonas aeruginosa DnaB Helicase to the Plasmid Origin.
	jvi.asm.org /cgi/content/abstract/74/9/4057 (332 words)

	The DnaC helicase loader is a dual ATP/ADP switch protein -- Davey et al. 21 (12): 3148 -- The EMBO Journal (Site not responding. Last check: 2007-10-20)
		DnaB (4.4 pmol) assembly was performed in the presence of 25 pmol wild-type DnaC and 50 pmol DnaC mutant (open circles) or 75 pmol wild-type DnaC (filled circles).
		Substrate, the indicated nucleotide(s), DnaB (4.4 pmol hexamer, when present) and DnaC (indicated amounts) were incubated at 37°C before separating free oligonucleotide from intact substrate by electrophoresis through a 15% polyacrylamide gel (in Tris–borate).
		HU (Parada and Marians, 1991), DnaB, SSB and [
	embojournal.npgjournals.com /cgi/content/full/21/12/3148 (8034 words)

	[No title] (Site not responding. Last check: 2007-10-20)
		Kleinsteuber S., Quinones A. (1995) Expression of the dnaB gene of Escherichia coli is inducible by replication-blocking DNA damage in a recA-independent manner.
		Nakayama N., Arai N., Bond M.W., Kaziro Y., Arai K. (1984) Nucleotide sequence of dnaB and the primary structure of the dnaB protein from Escherichia coli.
		Gunther E., Bagdasarian M., Schuster H. (1984) Cloning of the dnaB gene of Escherichia coli: the dnaB gene of groPB534 and groPB612 and the replication of phage lambda.
	bmb.med.miami.edu /EcoGene/EcoWeb/CESSPages/Refs/Eg10236.htm (1115 words)

	Pfam 18.0 : DnaB_2 (Site not responding. Last check: 2007-10-20)
		The DnaB protein is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110 in Bacillus subtilis.
		It is known that there are two different classes (DnaBI and DnaBII) in the DnaB mutants; DnaBI is essential for both chromosome and pUB110 replication, whereas DnaBII is necessary only for chromosome replication MEDLINE:3027697.
		Nucleotide sequence of Bacillus subtilis dnaB: a gene essential for DNA replication initiation and membrane attachment.
	pfam.wustl.edu /cgi-bin/getdesc?name=DnaB_2 (194 words)

	biology - DnaB helicase
		Have a Question or Comment on this Article?
		dnaB helicase is an enzyme which holds open the replication fork during DNA replication.
		Initially when dnaB binds to dnaA, it is associated with dnaC.
	www.biologydaily.com /biology/DnaB (48 words)

	AA1060 (Site not responding. Last check: 2007-10-20)
		DnaB [Pasteurella multocida] gi20137939spQ9CNL6DNAB_PASMU Replicative DNA helicase gi12720663gbAAK02496.1
		replicative DNA helicase DnaB [Escherichia coli O157:H7] gi25299870pirB91258 replicative DNA helicase DnaB [imported] - Escherichia coli (strain O157:H7, substrain RIMD 0509952) gi25299878pirF86098 replicative DNA helicase, part of primosome [imported] - Escherichia coli (strain O157:H7, substrain EDL933) gi12519003gbAAG59250.1AE005638_4 replicative DNA helicase; part of primosome [Escherichia coli O157:H7 EDL933] gi13364511dbjBAB38457.1
		dnaB; replicative DNA helicase protein [Nitrosomonas europaea ATCC 19718] gi30180103embCAD84105.1
	hpv-web.lanl.gov /oragen/bacteria/aact/blast_results/AA1060.html (552 words)

	InBase, Mcjw1 DnaB Intein (Site not responding. Last check: 2007-10-20)
		To access your account, log in or register.
		Mcjw1 DnaB intein (including -1 and +1 extein residues)
		Note that the Momega and Mcjw1 DnaB inteins are very similar, but inserted into different K/T sites in the phage DnaB extein.
	tools.neb.com /inbase/intein.php?name=Mcjw1+DnaB (69 words)

	Energy Citations Database (ECD) - Energy and Energy-Related Bibliographic Citations (Site not responding. Last check: 2007-10-20)
		Energy Citations Database (ECD) Document #7176638 - Properties of DnaB helicase in [lambda] DNA replication
		Availability information may be found in the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or via the "Full-text Availability" link.
		Properties of DnaB helicase in [lambda] DNA replication
	www.osti.gov /energycitations/product.biblio.jsp?osti_id=7176638 (92 words)

	UniProtKB/Swiss-Prot entry P03005 [DNAB_ECOLI] Replicative DNA helicase
		They link to the user manual or other documents.
		Nakayama N. Arai N. Bond M.W. Kaziro Y. Arai K. "Nucleotide sequence of dnaB and the primary structure of the dnaB protein from Escherichia coli.";
		Weigelt J. Brown S.E. Miles C.S. Dixon N.E. Otting G. "NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer.";
	www.expasy.org /uniprot/P03005 (359 words)

	dnaB
		Information for dnaB, and also upstream and downstream flanking genes:
		Species with an ortholog: HI PA SP ST EC Predictions with an average map value >= the critical MAP value (p=0.05):
		Predictions with an average map value < the critical MAP value (p=0.05):
	bayesweb.wadsworth.org /binding_sites/dnaB.html (43 words)

	InBase, Momega DnaB Intein (Site not responding. Last check: 2007-10-20)
		Home > Technical Reference > InBase > Momega DnaB Intein
		Momega DnaB intein (including -1 and +1 extein residues)
		Obtained from the NCBI protein database using accession number AAN12655.1
	tools.neb.com /inbase/intein.php?name=Momega+DnaB (69 words)

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