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Topic: Edman degradation

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	AAS-Biographical memoirs-Edman
		Edman synthesized the PTH derivatives of all the amino acids found in proteins and developed chromatographic systems to identify and quantify them; conditions for the coupling of PITC to the amino terminus and the cleavage of the PTH derivative which worked smoothly for all peptide bonds were found.
		During the next few years Edman's aim was to improve the repetitive yield obtained from the machine; an increase from the 98% of the 1967 paper to 99% was calculated to double the length of determinable sequence.
		Edman was elected a Fellow of the Australian Academy of Scienec in 1968 and a Fellow of the Royal Society of London in 1974.
	www.science.org.au /academy/memoirs/edman.htm (2620 words)

	Edman degradation - Wikipedia, the free encyclopedia
		Edman degradation, developed by Pehr Edman, is a method of sequencing amino acids in a peptide.
		This is because the Edman degradation reaction is not 100% efficient, meaning that the cleavage step does not occur every time.
		An advantage of the Edman degradation is that it only uses 10 - 100 picomoles of peptide for the sequencing process.
	en.wikipedia.org /wiki/Edman_degradation (253 words)

	Method for compositional tag sequencing - Patent 6277644
		The amino acids released from the Edman degradation reactions are then analyzed to determine a partial amino acid sequence, the results of which are correlated with sequence data in the existing DNA and protein databases.
		The invention enables the Edman degradation chemistry to be separated from amino acid analysis, and allows multiple cycles of coupling and cleavage to be performed prior to amino acid extractions.
		Edman degradation can be performed on the N-terminus of a protein, or the protein can be cleaved into peptides in which case the Edman degradation is performed from the N-terminus of peptides.
	www.freepatentsonline.com /6277644.html (3919 words)

	Edman Sequencing Info
		The Edman Sequencing Service offers two services for protein characterization and identification: 1) N-terminal sequence analysis using automated Edman degradation chemistry and 2) internal protein sequence analysis by Edman degradation chemistry after in-gel or PVDF bound protein digestions followed by HPLC separation of the resulting peptides (1-3).
		The initial yield (typically 50-80% based on standard proteins) refers to the quantity of amino acid recovered in the first cycle of the Edman chemistry and is expressed as a percentage of the total sample analyzed.
		In the case of internal Edman sequencing samples, if an initial database search using the MALDI-TOF MS data of the unfractionated digestion mixture yields any significant candidates for protein identity, these results will be discussed with the investigator prior to further analysis of the samples in order to determine the best course for further analysis.
	pdtc.rockefeller.edu /Edman/Edmaninfo.html (3095 words)

	Pehr Edman - Wikipedia, the free encyclopedia
		At the time Edman started working on Angiotensin, it was just being recognized that proteins are distinct entities with a defined molecular mass, electric charge and structure.
		This inspired Edman to develop a method, that could be used to determine the sequence of amino acids in the protein.
		In 1950 he published his first paper using the method later known as Edman degradation, to determine the sequence of a protein.
	en.wikipedia.org /wiki/Pehr_Edman (358 words)

	NIAID: Research Technologies Branch: Protein Sequencing
		Edman degradation relies on both the high chemical reactivity of a protein's terminal amino acid and the ability to remove the selectively derived amino acid from the protein while leaving the rest of the peptide chain intact.
		Each cycle of the degradation occurs at the newly formed amino-terminal amino acid left by the preceding degradation.
		The sequencing process is not completed by Edman degradation alone.
	www.niaid.nih.gov /Dir/services/rtb/protein/techEdman.asp (191 words)

	Automated Interpretation of Edman Sequencing Data
		Edman degradation is a method for determining the amino acid sequence of a peptide.
		Studies indicate that automatic interpretation of sequence data derived by this method is woefully inadequate.
		Daniel H. Wagner Associates developed statistical algorithms and software to automatically determine the amino acid sequence of a peptide from the experimental output of Edman degradation.
	www.wagner.com /technologies/biotech/edman.html (231 words)

	Analytical Techniques: Peptide Tag
		Since the Edman degradation requires a free N-terminus, this peptide as code strategy could also be used to encode other peptide by acylating the N-terminus of the binding peptide strand, and leaving a free amine at the coding peptide terminus.
		To accommodate the parallel synthesis of both binding and coding peptides, an orthogonally protected bifunctional linker was employed that contained both acid and base sensitive protecting groups.
		This places a severe restriction on the scope of the synthetic techniques that may be applied during library synthesis, and restricts the synthesis of more pharmaceutically attractive small organic molecules.
	www.combichemistry.com /determination_methods_peptide_tag.html (209 words)

	Attomole level protein sequencing by Edman degradation coupled with accelerator mass spectrometry -- Miyashita et al. ...
		Attomole level protein sequencing by Edman degradation coupled with accelerator mass spectrometry -- Miyashita et al.
		Attomole level protein sequencing by Edman degradation coupled with accelerator mass spectrometry
		Edman degradation remains the primary method for determining the sequence of proteins.
	www.pnas.org /cgi/content/abstract/98/8/4403 (228 words)

	Edman Degradation (Site not responding. Last check: 2007-11-03)
		Home > Peptide Sequencing Techniques > Edman Degradation - amino acid sequencing
		In future PepSeq may be updated to include Edman Degradation simulation.
		If you would like a computer simulation of Edman Degradation please contact me using the Register Interest Form.
	www.pepseq.com /peptide-sequencing/Edman-degradation (263 words)

	Edman degradation (Site not responding. Last check: 2007-11-03)
		A lab technique used to find out the order of amino acids in a polypeptide (chain of amino acids).
		It involves using the Edman reagent, phenyl isothiocyanate (PITC), to react one by one with each amino acid, in order.
		The technique is used in machines which automatically sequence (determine the order of subunits) polypeptides.
	www.cartage.org.lb /en/themes/Reference/dictionary/Biologie/E/11.html (54 words)

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