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Topic: Endosome

In the News (Sat 12 Dec 09)

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Mickey Rourke

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Hugh Jackman

	Prion endosome recycling and FTIR strain differentiation
		Endosomal enlargement contributed to an average 2.5-fold larger total endosomal volume per neuron, implying a marked increase in endocytic activity.
		The endosome fraction was shown to be heterogeneous and consisted of two vesicle populations, differing in density properties and iron content.
		Syntaxin 7, a novel syntaxin member associated with the early endosomal compartment is 35, 34, 34, 34, 25, and 19% identical to syntaxins 1, 2, 3, 4, 5, and 6.
	www.mad-cow.org /jan99_sci_news.html (5501 words)

	Mitotic phosphorylation of rab4 prevents binding to a specific receptor on endosome membranes (Site not responding. Last check: 2007-10-09)
		Endosome containing membranes were incubated with increasing concentrations of rab4−GDI (0, 10, 25, 50, 100 and 150 nM rab4).
		However, in intact cells, rab4 never co-localizes with lysosomal or late endosomal markers (van der Sluijs et al., 1992; Daro et al., 1996), and in vitro, fractions from dense regions of the sucrose gradients that were relatively enriched in lysosomes and depleted in endosomes did not bind rab4.
		Although rab4 phosphorylation appeared to inhibit the binding of rab4 to interphase endosome membranes, it remained possible that an additional modification of the membranes occurs in mitotic cells that further regulates their ability to interact with rab4.
	www.nature.com /cgi-taf/DynaPage.taf?file=/emboj/journal/v16/n15/full/7590428a.html (9190 words)

	Nikon MicroscopyU: Fluorescence Microscopy - EYFP Endosome Subcellular Localization
		In eukaryotic cells, endosomes constitute a large network of cytoplasmic vesicles that are formed through the fusion of smaller internalized vesicles arising from receptor-mediated endocytosis.
		Often, the endosomal vesicles ultimately transfer their contents to the lysosomes for processing, but this is not always the case.
		Intracellular endosomal networks labeled with resident macromolecules containing fluorescent protein domains, such as GFP or EYFP, can be readily visualized using fluorescence microscopy, as illustrated for a variety of established adherent cell lines in Figure 1.
	www.microscopyu.com /articles/fluorescence/filtercubes/yfp/yfphyq/endosomes/yfpendosomesindex.html (988 words)

	Inhibition of Endosome Fusion by Wortmannin Persists in the Presence of Activated rab5 -- Jones et al. 9 (2): 323 -- ...
		Inhibition of Endosome Fusion by Wortmannin Persists in the Presence of Activated rab5 -- Jones et al.
		Inhibition of Endosome Fusion by Wortmannin Persists in the Presence of Activated rab5
		Rab5-dependent endosome fusion is sensitive to the phosphoinositide 3-kinase inhibitor, wortmannin.
	www.molbiolcell.org /cgi/content/full/9/2/323 (5317 words)

	Hrs regulates early endosome fusion by inhibiting formation of an endosomal SNARE complex -- Sun et al. 162 (1): 125 -- ...
		Membranes were obtained as described for homotypic reactions of early endosomes (see Materials and methods) and fixed in 3% glutaraldehyde (A, donor) or after incubation with acceptor membranes, cytosol, and ATP regenerating system (B, after fusion).
		and sufficient for the inhibition of endosome fusion.
		Characterization of the early endosome and putative endocytic carrier vesicles in vivo and with an assay of vesicle fusion in vitro.
	www.jcb.org /cgi/content/full/162/1/125 (8509 words)

	Receptor mediated endocytosis II
		The receptor may be recycled to the surface by vesicles that bud from the endosome and then target the plasma membrane.
		Perhaps this is stimulated by the concentration of cholesterol in the late endosomes.
		This appears to block retrograde transport from the endosome to the Golgi complex, as evidence by the accumulation of the MPR.
	anatomy.utmb.edu /cellbio/recend2.htm (2596 words)

	A Novel Membrane-anchored Rab5 Interacting Protein Required for Homotypic Endosome Fusion -- Hoffenberg et al. 275 ...
		The ras-related GTPase rab5 is rate-limiting for homotypic early endosome fusion.
		Endosome Fusion Assays-- These were performed as described previously (12, 34).
		Endosome fusions were performed as described previously (12), with 1 mg/ml cytosol and endosome membranes.
	www.jbc.org /cgi/content/full/275/32/24661 (5420 words)

	Ferrichrome induces endosome to plasma membrane cycling of the ferrichrome transporter, Arn1p, in Saccharomyces ... (Site not responding. Last check: 2007-10-09)
		Ferrichrome induces endosome to plasma membrane cycling of the ferrichrome transporter, Arn1p, in Saccharomyces cerevisiae -- Kim et al.
		Ferrichrome induces endosome to plasma membrane cycling of the ferrichrome transporter, Arn1p, in Saccharomyces cerevisiae
		In step 1, the presence of extracellular ferrichrome is signaled to Arn1p as it cycles between the early and late endosome, which results in translocation of Arn1p to the plasma membrane.
	embojournal.npgjournals.com /cgi/content/full/21/14/3632 (6824 words)

	Na+-H+ exchanger 3 (NHE3) is present in lipid rafts in the rabbit ileal brush border: a role for rafts in trafficking ...
		EEA1 (early endosomal autoantigen 1) is present on the surface of early endosomes.
		Early endosomes were isolated by incubating partially purified early endosomes with magnetic beads (Dynabeads, M-500 subcellular, from Dynal) coated with anti-EEA1 monoclonal antibody (EEA1 mAb) according to the manufacturer's protocol.
		Early endosomes were immunoisolated using EEA1 mAb-coated Dynal M500 magnetic beads from fractions 1 and 2 of the OptiPrep gradients as described in Fig.
	jp.physoc.org /cgi/content/full/537/2/537 (9394 words)

	A Membrane Coat Complex Essential for Endosome-to-Golgi Retrograde Transport in Yeast -- Seaman et al. 142 (3): 665 -- ...
		acidic lumen of the endosome triggers release of hydrolases that
		Excess cold methionine/cysteine was then added, and the cells were chased for 45 min, after which the cells were lysed in Hepes buffer and the lysate was spun at 2,000 rpm to remove unbroken cells.
		Labeling was found to be restricted to discrete regions of the prevacuolar endosomal membrane (Ec), and appeared most concentrated at the swollen rim of the cisterna (B).
	www.jcb.org /cgi/content/full/142/3/665 (10526 words)

	STAM Proteins Bind Ubiquitinated Proteins on the Early Endosome via the VHS Domain and Ubiquitin-interacting Motif -- ...
		Bishop, N., Horman, A., and Woodman, P. Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates.
		Komada, M., Masaki, R., Yamamoto, A., and Kitamura, N. Hrs, a tyrosine kinase substrate with a conserved double zinc finger domain, is localized to the cytoplasmic surface of early endosomes.
		Komada, M., and Soriano, P. Hrs, a FYVE finger protein localized to early endosomes, is implicated in vesicular traffic and required for ventral folding morphogenesis.
	www.molbiolcell.org /cgi/content/full/14/9/3675 (7611 words)

	Retromer function in endosome-to-Golgi retrograde transport is regulated by the yeast Vps34 PtdIns 3-kinase -- Burda et ...
		FM4-64-labeling of vacuoles and endosomes and fluorescence microscopy
		the endosome and was identified by the isolation of mutants
		mislocalized from endosomal membranes to the cytosol in
	jcs.biologists.org /cgi/content/full/115/20/3889 (6317 words)

	When cell biology meets development: endocytic regulation of signaling pathways -- Seto et al. 16 (11): 1314 -- Genes ...
		The early endosome accepts and delivers proteins and lipids from multiple membrane-bound compartments, including the plasma membrane (via recycling endosomes and clathrin-coated vesicles, CCVs), the Golgi (via transport vesicles from the trans Golgi network, TGN), and lysosome/vacuole (via late endosomes/multivesicular bodies, MVBs).
		Ubiquitination of RTKs by Cbl serves as a signal for degradation in the lysosome whereas nonubiquitinated RTKs are recycled to the surface.
		It is likely during endosomal sorting that the ultimate fate of receptors and the impact of endocytosis on signaling are determined.
	www.genesdev.org /cgi/content/full/16/11/1314 (8031 words)

	Late endosome motility depends on lipids via the small GTPase Rab7 -- Lebrand et al. 21 (6): 1289 -- The EMBO Journal (Site not responding. Last check: 2007-10-09)
		Rab7 overexpression caused endosome accumulation in the perinuclear
		Gruenberg,J., Griffiths,G. and Howell,K.E. (1989) Characterization of the early endosome and putative endocytic carrier vesicles in vivo and with an assay of vesicle fusion in vitro.
		Mullock,B.M., Bright,N.A., Fearon,C.W., Gray,S.R. and Luzio,J.P. (1998) Fusion of lysosomes with late endosomes produces a hybrid organelle of intermediate density and is NSF dependent.
	embojournal.npgjournals.com /cgi/content/full/21/6/1289 (5891 words)

	Outline for Lecture #6
		Vesicle is acidified to become endosome (or fuses with pre-existing endosome), and sorting of receptor(s) and ligand(s) begins.
		A single endosome may contain many different receptors and ligands, and different ones are sorted differently.
		Note: endosome may not simply split in one step; process of sorting may be gradual.
	www.columbia.edu /cu/biology/courses/c2006/lectures05/lect6.05.html (2381 words)

	Cleavage of Rabaptin-5 blocks endosome fusion during apoptosis -- Cosulich et al. 16 (20): 6182 -- The EMBO Journal (Site not responding. Last check: 2007-10-09)
		of endosomes and inhibition of the endocytic pathway during the
		Inhibition of endosome fusion and cleavage of Rabaptin-5 are accelerated by caspase-3.
		of crude endosomal membranes (Woodman and Warren, 1989
	embojournal.npgjournals.com /cgi/content/full/16/20/6182 (5593 words)

	Deletion of the SNARE vti1b in Mice Results in the Loss of a Single SNARE Partner, Syntaxin 8 -- Atlashkin et al. 23 ...
		endosomes and autophagic vacuoles was observed in hepatocytes
		A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function.
		Differential roles of syntaxin 7 and syntaxin 8 in endosomal trafficking.
	mcb.asm.org /cgi/content/full/23/15/5198 (5439 words)

	Distribution and trafficking of MPR300 is normal in cells with cholesterol accumulated in late endocytic compartments: ...
		to endosomal compartments caused by a disruption of the gene
		A role for the lysosomal membrane protein LGP85 in the biogenesis and maintenance of endosomal and lysosomal morphology.
		The kinetics of mannose 6-phosphate receptor trafficking in the endocytic pathway in Hep-2 cells: the receptor enters and rapidly leaves multivesicular endosomes without accumulating in a prelysosomal compartment.
	www.jlr.org /cgi/content/full/44/10/1821 (6520 words)

	Huntingtin Expression Stimulates Endosomal-Lysosomal Activity, Endosome Tubulation, and Autophagy -- Kegel et al. 20 ...
		Lippincott-Schwartz J, Yuan L, Tipper C, Amherdt M, Orci L, Klausner RD (1991) Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic.
		Tooze J, Hollinshead M (1992) In AtT20 and HeLa cells brefeldin A induces the fusion of tubular endosomes and changes their distribution and some of their endocytic properties.
		Yoshimori T, Yamagata F, Yamamoto A, Mizushima N, Kabeya Y, Nara A, Miwako I, Ohashi M, Ohsumi M, Ohsumi Y (2000) The mouse SKD1, a homologue of yeast vps4p, is required for normal endosomal trafficking and morphology in mammalian cells.
	www.jneurosci.org /cgi/content/full/20/19/7268 (7735 words)

	Lysosomes and Peroxisomes
		In this case, the early coalescence of vesicles bringing in the receptor and ligand produces an endosome.
		The blue and green lysosomes are probably endosomes.
		Therefore, one can follow entry of the receptor-ligand complex and then see the fluorescence disappear as the endosome containing the complex is acidified.
	www.cytochemistry.net /Cell-biology/lysosome.htm (702 words)

	Cell-free Reconstitution of Transport from the trans-Golgi Network to the Late Endosome/Prevacuolar Compartment -- ...
		(44) and to undergo transport from early endosomes to the PVC
		endosome as Kex2p donor compartment is challenging because of
		appear to traffic through early endosomes (14, 15, 45).
	www.jbc.org /cgi/content/full/279/47/48767 (3833 words)

	Anatomy and trafficking pathways of the late endosome
		Anatomy and trafficking pathways of the late endosome.
		(A) Distribution of several proteins from mammalian cells or yeast in the late endosome or in yeast vacuoles that reflect the enrichment of proteins on lumenal vesicles or the limiting membrane in the MVB.
		These machinery sets control the fusion of late endosomes, the formation of internal vesicles that accumulate in the late endosome, and recycling out of the late endosome.
	info.med.yale.edu /intmed/infdis/joiner/endosome_pathway.html (187 words)

	Intracellular kinetics of iron in reticulocytes: evidence for endosome involvement in iron targeting to mitochondria -- ... (Site not responding. Last check: 2007-10-09)
		Intracellular kinetics of iron in reticulocytes: evidence for endosome involvement in iron targeting to mitochondria -- Zhang et al.
		Intracellular kinetics of iron in reticulocytes: evidence for endosome involvement in iron targeting to mitochondria
		endosomes into heme, suggesting that myosin is required for
	www.bloodjournal.org /cgi/content/abstract/105/1/368 (311 words)

	Dynamics of rab5 activation in endocytosis and phagocytosis -- Roberts et al. 68 (5): 627 -- Journal of Leukocyte ...
		EGF caused a rapid increase in endosome fusion and in membrane
		Endosome and pinosome fusion were common in EGF-stimulated cells.
		in the structure and activity of the early endosomal and early
	www.jleukbio.org /cgi/content/abstract/68/5/627 (748 words)

	The Ubiquitin-Proteasome System Facilitates the Transfer of Murine Coronavirus from Endosome to Cytoplasm during Virus ...
		An aliquot of PNS before sucrose gradient sedimentation was used to quantitate the total amount of viral RNA in the treated and untreated cells (right gel).
		1 to 3, the reported late endosome fractions (6).
		release the virion from the endosome is still unclear.
	jvi.asm.org /cgi/content/full/79/1/644 (2644 words)

	Receptor (CD155)-Dependent Endocytosis of Poliovirus and Retrograde Axonal Transport of the Endosome -- Ohka et al. 78 ...
		Receptor (CD155)-Dependent Endocytosis of Poliovirus and Retrograde Axonal Transport of the Endosome
		The hPVR CP on the surfaces of endosomes
		The endosomes are transported by dynein along microtubules, and the viral genomic RNA transfers into the cytoplasm.
	jvi.asm.org /cgi/content/full/78/13/7186 (5966 words)

	Autoantigen Golgin-97, an Effector of Arl1 GTPase, Participates in Traffic from the Endosome to the Trans-Golgi Network ...
		The involvement of Arl1 in endosome to TGN retrograde trafficking
		Mallet, W.G., and Maxfield, F.R. Chimeric forms of furin and TGN38 are transported with the plasma membrane in the trans-Golgi network via distinct endosomal pathways.
		Straley, K.S. and Green, S.A. Rapid transport of internalized P-selectin to late endosomes and the TGN: roles in regulating cell surface expression and recycling to secretory granules.
	www.molbiolcell.org /cgi/content/full/15/10/4426 (8788 words)

	Syntaxins 13 and 7 Function at Distinct Steps During Phagocytosis -- Collins et al. 169 (6): 3250 -- The Journal of ...
		lysosomes, but not at all with endosomes (13, 14).
		Syntaxin 13 colocalizes with the transferrin receptor in the recycling endosome
		Lysosomal enzyme trafficking between phagosomes, endosomes, and lysosomes in J774 macrophages: enrichment of cathepsin H in early endosomes.
	www.jimmunol.org /cgi/content/full/169/6/3250 (4801 words)

	Secretagogue-induced translocation of CRHSP-28 within an early apical endosomal compartment in acinar cells -- Thomas ...
		Secretagogue-induced translocation of CRHSP-28 within an early apical endosomal compartment in acinar cells -- Thomas et al.
		Articles by Thomas, D. Articles by Groblewski, G. Secretagogue-induced translocation of CRHSP-28 within an early apical endosomal compartment in acinar cells
		calcium signaling; membrane trafficking; early endosome; secretion; calcium/calmodulin-dependent protein kinase II Address for reprint requests and other correspondence: G. Groblewski, Dept. of Nutritional Sciences, Univ. of Wisconsin, 1415 Linden Dr., Madison, WI 53706 (E-mail: groby{at}nutrisci.wisc.edu).
	ajpgi.physiology.org /cgi/content/abstract/287/1/G253 (340 words)

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