| | HS2 Enhancer Function Is Blocked by a Transcriptional Terminator Inserted between the Enhancer and the Promoter. |
 | | In the endogenous genome of erythroid cells and in recombinant constructs transfected into erythroid cells, the HS2 enhancer recruits erythroid and general transcription factors (11-13) in the assembly of a pol II transcription complex that synthesizes intergenic RNAs from sites within the enhancer in the direction of the downstream gene (14-17). |
 | | To prevent tandem integration of the plasmids such that the HS2 enhancer in one plasmid could activate transcription of the CAT gene in the upstream, neighboring plasmid, the plasmids were co-integrated with fragmented K562 spacer DNAs by a modified calcium phosphate precipitation method (Ref. |
 | | In the 340-kb Drosophila bithorax complex where enhancers act over long intergenic distances to regulate transcription of distant cis-linked genes, the intergenic DNA was also transcribed, and interfering with synthesis of the intergenic RNAs disrupts transcription of the far downstream genes (34, 35). |
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