
	Where results make sense

Topic: Enzyme Linked Immunosorbent Assay

	Enzyme-linked immunosorbent assay for brucella antigen detection in human sera.
		Enzyme-linked immunosorbent assay for brucella antigen detection in human sera.
		A sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody coupled to the solid phase was evaluated for the detection of brucella antigen in serum samples.
		The standardised assay was performed on 1607 sera from random blood donors, 146 patients with brucellosis, 20 persons in high risk groups and 264 sera from patients with diseases other than brucellosis.
	www.medscape.com /medline/abstract/9879960?src=emed_ckb_ref_0 (241 words)

	ELISA - Wikipedia, the free encyclopedia
		The Enzyme-Linked ImmunoSorbent Assay (ELISA for short) is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample.
		It uses two antibodies, one of which is specific to the antigen and the other of which is coupled to an enzyme.
		Because the ELISA can be performed to evaluate either the presence of antigen or the presence of antibody in a sample, it is a useful tool both for determining serum antibody concentrations (such as with the Human Immunodeficiency Virus, HIV test or West Nile Virus) and also for detecting the presence of antigen.
	en.wikipedia.org /wiki/Enzyme-linked_immunosorbent_assay (776 words)

	Millipore - Immunodetection - ELISA - Enzyme-Linked Immunosorbent Assay
		To utilize this assay, one antibody (the ‘capture’ antibody) is purified and bound to a solid phase typically attached to the bottom of a plate well.
		The assay is then quantitated by measuring the amount of labeled second antibody bound to the matrix, through the use of a colorimetric substrate.
		The enzyme may be linked to either the immunogen or the primary antibody.
	www.millipore.com /markets/immunodetect.nsf/docs/elisa (1216 words)

	CDC - Detection by Enzyme-Linked Immunosorbent Assay of Antibodies to West Nile virus in Birds
		The PVP of this assay appears to be somewhat lower than that of another reported ELISA protocol (17) and some other flavivirus serologic assays, such as PRNT, but is higher than that reported for the assay from which it was derived (11).
		A new enzyme immunoassay to detect antibodies to arboviruses in the blood of wild birds.
		Detection of antibodies to alphaviruses by enzyme-linked immunosorbent assay.
	www.cdc.gov /ncidod/EID/vol8no9/02-0152.htm (2121 words)

	WHO \| Enzyme-linked immunosorbent assay (ELISA)
		In the rabies field the enzyme-linked immunosorbent assay (ELISA) was initially developed for the titration of rabies virus-neutralizing antibodies.
		The technique was applied to the quantification of rabies antigen by Atanasiu et al using fluorescein-labelled IgG to the purified nucleocapsid.
		Subsequently, Perrin et al developed an ELISA called rapid rabies enzyme immunodiagnosis (RREID), which was based upon the detection of rabies virus nucleocapsid antigen in brain tissue.
	www.who.int /entity/rabies/human/enzymeimmunoassay/en/index.html (274 words)

	Healthopedia.com - ELISA (Enzyme-Linked Immunosorbent Assay)
		An enzyme is a protein that speeds up a chemical reaction.
		In this reaction, the enzyme causes the test solution to change color, which is a positive result.
		Links to other sites are provided for information only -- they do not constitute endorsements of those other sites.
	www.healthopedia.com /elisa (683 words)

	Scientist Solutions - Sandwich Enzyme Linked Immunosorbent Assay
		Enzyme-linked Immunosorbent Assays (ELISAs) combine the specificity of antibodies with the sensitivity of simple enzyme assays, by using antibodies or antigens coupled to an easily assayed enzyme that possesses a high turnover number.
		To utilize this assay, one antibody (the “capture” antibody) is purified and bound to a solid phase typically attached to the bottom of a plate well.
		The assay is then quantitated by measuring the amount of labeled second antibody bound to the matrix, through the use of a colorimetric substrate.
	www.scientistsolutions.com /index.php?a=topic&t=3062 (398 words)

	Cereal Research Centre - The Enzyme-linked Immunosorbent Assay (ELISA) (Site not responding. Last check: 2007-10-26)
		This is a serological assay most often run in 96-well microtitre plates, where an enzyme-linked antibody is used to bind to a target-specific antibody and give a colour change.
		The intent is to develop an assay which will be able to detect and quantify levels of beta glucans in oat and barley seed and flour.
		The assay should be capable of handling large numbers of samples, should be inexpensive and easy to use.
	res2.agr.ca /winnipeg/mg_bx1_e.htm (160 words)

	Enzyme Linked Immunosorbent Assay
		The Enzyme Linked Immunosorbent Assay (ELISA), (Enzyme Immunoassay (EIA) or Solid-Phase Immuosorbent Assay (SPIA)) is a sensitive laboratory method used to detect the presence of antigens (Ag) or antibodies (Ab) of interest in a wide variety biological samples.
		It is important, during assay optimisation, to ensure that the secondary Ab does not bind non-specifically to the Ag preparation or impurities within it, nor to the solid phase.
		The assay is ideally designed if the maximum number of binding sites on the solid phase are taken up by an Ag which is in the correct conformation to optimally bind Ab.
	www.uq.edu.au /vdu/VDUELISA.htm (1078 words)

	ARS \| Publication request: A Modified Enzyme-Linked Immunosorbent Assay for Detection of Cutaneous Antibody Against ... (Site not responding. Last check: 2007-10-26)
		The assays currently used to measure the cutaneous anti-Ich antibody are not sensitive enough for low levels of antibody.
		The purpose of this study was to modify and optimize an enzyme-linked immunosorbent assay (ELISA) for detection of low levels of cutaneous antibody against Ich in channel catfish and in water.
		The original assay failed to detect the antibody even though the water samples were concentrated 40-fold.
	www.ars.usda.gov /research/publications/Publications.htm?seq_no_115=141891 (504 words)

	Bioline International Official Site (site up-dated regularly)
		Evaluation of the dot enzyme-linked immunosorbent assay in comparison with standard ELISA for the immunodiagnosis of human toxocariasis
		A total of 0.2 ng of antigen per dot, serum dilution of 1:160 and dilution conjugate of 1:1000 were found optimal.
		The sensitivity and specificity of the assay were 100 and 95%, respectively.
	www.bioline.org.br /abstract?id=oc06013 (160 words)

	Evaluation of a Commercial Capture Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin M and G Antibodies ... (Site not responding. Last check: 2007-10-26)
		assay for the serological diagnosis of dengue infections.
		By using the HAI assay as the reference test, 90 patients were diagnosed as having primary dengue (circles), 58 were diagnosed as having secondary dengue (squares), and 28 had no evidence of dengue infection (triangles).
		An enzyme-linked immunosorbant assay to characterise dengue infections where dengue and Japanese encephalitis co-circulate.
	cdli.asm.org /cgi/content/full/5/1/7 (2665 words)

	ELISA Summary
		1: an assay that relies on an enzymatic conversion reaction and is used to detect the presence of specific substances (such as enzymes or viruses or antibodies or bacteria) [syn: enzyme-linked-immunosorbent serologic assay]
		The enzyme-linked immunosorbant assay, which is commonly abbreviated to ELISA, is a technique that promotes the binding of the target antigen or antibody to a substrate, followed by the binding of an enzyme-linked molecule to the bound antigen or antibody.
		The Enzyme-Linked ImmunoSorbent Assay (ELISA for short) is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample.
	www.bookrags.com /ELISA (1290 words)

	[No title] (Site not responding. Last check: 2007-10-26)
		Since only free enzyme-labeled drug molecules can act on the substrate, enzyme activity is reduced.The amount of free enzyme left in the mixture determines the amount of substrate that is converted to a colored form from an uncolored one, in a given time period.
		The first is that the enzyme need to remain active after it binds to the compound being measured (hapten), and secondly when the hapten reacts with its specific antibody the enzyme activity of the hapten-enzyme conjugate is either reduced or inhibited.
		The antigen competitive inhibition assay begins with an antibody bound to a polystyrene well (see fig) plus a test sample containing an antigen mixture to which an antigen-enzyme conjugate is added (see fig).
	kerouac.pharm.uky.edu /HomeTest/Elisa/elisa.html (667 words)

	Immunoblotting and Enzyme-Linked Immunosorbent Assay for the Diagnosis of Pemphigoid Gestationis -- Sitaru et al. 103 ...
		Immunoblotting and Enzyme-Linked Immunosorbent Assay for the Diagnosis of Pemphigoid Gestationis
		The enzyme-linked immunosorbent assay with a recombinant form of the immunodominant domain of bullous pemphigoid antigen 180 is a specific and sensitive tool for the diagnosis of pemphigoid gestationis.
		Clinical significance of enzyme-linked immunosorbent assay for the detection of circulating anti-BP180 autoantibodies in patients with bullous pemphigoid.
	www.greenjournal.org /cgi/content/full/103/4/757 (3163 words)

	ARS \| Publication request: Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for the Insecticide Imidacloprid. (Site not responding. Last check: 2007-10-26)
		Citation: Kim, H., Shelver, W.L., Li, Q.X. Monoclonal antibody-based enzyme-linked immunosorbent assay for the insecticide imidacloprid.
		This antibody was utilized to develop an enzyme linked immunoassay toward imidacloprid.
		Technical Abstract: An enzyme-linked immunosorbent assay (ELISA) was developed for the neonicotinoid insecticide imidacloprid, 1-[(6-chloro-3-pyridinyl)methyl]-N-nitro-2-imidazolidinimine based on monoclonal antibody (MAb).
	www.ars.usda.gov /research/publications/publications.htm?seq_no_115=148458 (352 words)

	Bioline International Official Site (site up-dated regularly)
		Enzyme-linked Immunosorbent Assay for Detection and Quaffigntification of Cryptococcus neoformans Antigen
		ABSTRACT: An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid.
		A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples.
	www.bioline.org.br /abstract?id=oc01035 (141 words)

	World J Gastroenterol
		Thus, the present study intended to develop and evaluate an enzyme-linked immunosorbent assay for mite-specific antibody detection in serum samples using Dermatophagoides farinae extract.
		Then the plate was washed with PBS (pH=7.4), sera of the patients with intestinal acariasis were diluted at 1:40 and added in duplicated wells, and incubated in water bath at 37 °C for 60 min.
		Enzyme linked immunosorbent assay (ELISA) for the detection of IgG,
	www.wjgnet.com /1007-9327/10/1369.asp (1807 words)

	IngentaConnect Development of an Enzyme-Linked Immunosorbent Assay Method To Det... (Site not responding. Last check: 2007-10-26)
		An enzyme-linked immunosorbent assay for the detection of mustard protein was developed.
		The assay is based on a polyclonal antiserum directed against a mixture of mustard proteins raised in rabbits.
		The assay has a detection limit of 1.5 ppm (milligrams per kilogram) and is suitable for the detection of traces of mustard protein in mustard seed-derived flavoring ingredients.
	www.ingentaconnect.com /content/iafp/jfp/2007/00000070/00000001/art00028 (313 words)

	Detection of anti-Yt antibodies using a sensitive and specific enzyme-linked immunosorbent assay British Journal of ... (Site not responding. Last check: 2007-10-26)
		Abstract: A specific, sensitive and semi-quantitative enzyme-linked immunosorbent assay (ELISA) is described to detect anti-Yt^sup a^ antibodies in human serum.
		The enzyme protein is implicated in Alzheimer's disease,11-14 where AChE plaques within specific regions of the brain may have a role in the aetiology of this disease.
		This condition is characterised by the loss of motor neurons, particularly in the spinal cord and motor cortex of the brain, leading to neurogenic atrophy of the skeletal muscles, physical weakness, paralysis and respiratory failure.
	www.findarticles.com /p/articles/mi_qa3874/is_199901/ai_n8837301 (679 words)

	Enzyme-linked immunosorbent assay (ELISA) definition - Medical Dictionary definitions of popular medical terms
		Enzyme-linked immunosorbent assay (ELISA): ELISA stands for "enzyme-linked immunosorbent assay." This is a rapid immunochemical test that involves an enzyme (a protein that catalyzes a biochemical reaction).
		A mixture of purified HCG linked (coupled) to an enzyme and the test sample (blood, urine, etc) are added to the test system.
		The substance the enzyme acts on is then added, and the amount of product measured in some way, such as a change in color of the solution.
	www.medterms.com /script/main/art.asp?articlekey=9100 (278 words)

	Enzyme Linked Immunosorbent Assay
		Radial Immunodiffusion Assay (RID) is a specialized form of diffusion in agar in which immunoglobulin that recognizes specific antibody classes or subclasses is mixed with the molten agar before it is poured into the assay plate.
		The standard curve for the RID assay is generated using the values for the known standards.
		This is an example of a relatively simple, low cost assay that is still in use because it works and requires no special equipment or radioactivity.
	web.indstate.edu /thcme/PSP/labtests/radial.htm (244 words)

	Immunoglobulin M (IgM)-Glycoinositolphospholipid Enzyme-Linked Immunosorbent Assay: an Immunoenzymatic Assay for ... (Site not responding. Last check: 2007-10-26)
		Assays were performed without knowledge of the patient's status regarding levels of T.
		Enzyme-linked immunosorbent assay for antibodies to Toxoplasma gondii polysaccharides in human toxoplasmosis.
		An enzyme-linked immunosorbent assay for detection of IgM antibodies of Toxoplasma gondii: use for diagnosis of acute acquired toxoplasmosis.
	jcm.asm.org /cgi/content/full/40/4/1400 (3226 words)

	An Enzyme-Linked Immunosorbent Assay for the Measurement of Circulating Antibodies to Polymorphic Epithelial Mucin ...
		An Enzyme-Linked Immunosorbent Assay for the Measurement of Circulating Antibodies to Polymorphic Epithelial Mucin (MUC1)
		The objective of this study was to develop a reliable assay for the accurate determination of circulating free antibodies to MUC1.
		Material and Methods: We developed an enzyme-linked immunosorbent assay (ELISA) (PEM.CIg) employing a 60 mer peptide (a triple tandem repeat sequence of the MUC1 peptide core) conjugated to bovine serum albumin and peroxidase-labeled antihuman immunoglobulin G or M antibodies.
	content.karger.com /ProdukteDB/produkte.asp?Doi=30006 (396 words)

	Enzyme-Linked Immunosorbent Assay with Conserved Immunoreactive Glycoproteins gp36 and gp19 Has Enhanced Sensitivity ... (Site not responding. Last check: 2007-10-26)
		Enzyme-Linked Immunosorbent Assay with Conserved Immunoreactive Glycoproteins gp36 and gp19 Has Enhanced Sensitivity and Provides Species-Specific Immunodiagnosis of Ehrlichia canis Infection -- Cárdenas et al.
		The analytical sensitivity of the assay with the E.
		Comparison of three enzyme-linked immunosorbent assays with the indirect immunofluorescent antibody test for the diagnosis of canine infection with Ehrlichia canis.
	cvi.asm.org /cgi/content/full/14/2/123 (3933 words)

	Enzyme-Linked Immunosorbent Assay And Immunohistochemical Localisation Of Carcinoembryonic Antigen In Ovarian Neoplasia
		A monoclonal antibody based immunoassay kit which provides a quantitative measurement of CEA in human serum was used.
		It is a solid phase enzyme-linked immunosorbent assay based on sandwich principle.
		The difference in the technique of serum assay and the small number of borderline lesions in the current investigation may account for the difference in results.
	www.obgyn.net /hysteroscopy/articles/enzyme.htm (3923 words)

	Antibody Forum - Index
		The testing of sections of tissue for specific proteins by attatching them with specific antibodies, and then looking for the antibodies through the enzymes to which they were connected.
		One technique that is central to studying gene regulation and determining protein:DNA interactions is the electrophoretic mobility shift assay (EMSA).
		Measurement of the rate of a chemical reaction that takes place in the presence of an enzyme contained in a sample taken from an individual.
	www.antibodyforum.com (660 words)

Try your search on: Qwika (all wikis)