Where results make sense

About us   |   Why use us?   |   Reviews   |   PR   |   Contact us

W0152: Structures of a Self-Inhibitory Serine Protease and Its Pro-Enzyme: Activation Mechanism of Complement Factor D   (Site not responding. Last check: 2007-11-04) Factor D is a complement serine protease essential for the activation of complement alternative pathway. Pro-factor D displays an active catalytic triad conformation, flipped conformation for the self-inhibitory loop, and similar conformation for the flexible activation domain as that in trypsinogen, chymotrypsinogen, and prethrombin-2. Comparison between the structure of pro-enzyme and mature enzyme revealed the activation mechanism of complement factor D. www.hwi.buffalo.edu /ACA/ACA98/abstracts/text/W0152.html   (214 words)

Patent 5196306: Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation ... The enzyme, either by itself or in connection with a second enzyme, catalyzes the formation of an activated conjugate which then is deposited wherever a receptor for the activated conjugate is immobilized. One of the unique features of this invention is the analyte dependent enzyme activation system which catalyzes deposition of conjugate by converting the substrate portion of the conjugate to an activated form which is deposited wherever a specific receptor for the activated conjugate is immobilized. Other important factors include availability of the enzyme or enzymes, relative ease or difficulty of coupling it to the member of a specific binding pair, stability of the enzyme or enzymes as well as the stability of the conjugate and the receptor. www.freepatentsonline.com /5196306.html   (6880 words)

Activation of RNase L by 2',5'-Oligoadenylates. KINETIC CHARACTERIZATION -- Carroll et al. 272 (31): 19193 -- Journal ... of activator to enzyme monomer, the subsequent dimerization of -oligoadenylates is correlated with the dimerization of enzyme monomers. 1 indicate that the activation of the enzyme is reversible www.jbc.org /cgi/content/full/272/31/19193   (3571 words)

[No title] Since the lyophilized enzyme powder tends to stick to the glass wall of the reaction vessel, tyrosinase was immobilized on glass beads prior to equilibration at aw = 0.33.21 The effect of several macrocycles on the enzymatic activity of tyrosinase is given in Table VII. Although the activity of the enzyme lyophilized in the absence of crown ether is different due to differences in hydrophobicity and thermodynamic water activity of the media, the activation factor is almost identical in the three cases. We have shown that the (pre)treatment of enzymes with crown ethers is a simple and effective way to increase enzymatic activity in organic media up to a level which is only one to two orders of magnitude lower compared to their activity in water. homepages.strath.ac.uk /~cdbs05/engberg.htm   (4822 words)

Secretagogue-induced digestive enzyme activation and cell injury in rat pancreatic acini -- Saluja et al. 276 (4): 835 ... trypsinogen activation is mediated by the lysosomal enzyme cathepsin Pancreatic edema and intrapancreatic activation of trypsinogen during secretagogue-induced pancreatitis precedes glutathione depletion. Caerulein induced in vitro activation of trypsinogen in rat pancreatic acini is mediated by cathepsin B. Gastroenterology 113: 304-310, 1997 ajpgi.physiology.org /cgi/content/full/276/4/G835   (4864 words)

Regulation of Nitric Oxide-Sensitive Guanylyl Cyclase -- Friebe and Koesling 93 (2): 96 -- Circulation Research Restoration of the responsiveness of purified guanylate cyclase to nitrosoguanidine, nitric oxide, and related activators by heme and hemeproteins: evidence for involvement of the paramagnetic nitrosyl-heme complex in enzyme activation. Soluble guanylyl cyclase from bovine lung: activation with nitric oxide and carbon monoxide and spectral characterisation of the ferrous and ferric states. Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation. circres.ahajournals.org /cgi/content/full/93/2/96   (5435 words)

Activation of the furin endoprotease is a multiple-step process: requirements for acidification and internal propeptide ...   (Site not responding. Last check: 2007-11-04) Activation of the furin endoprotease is a multiple-step process: requirements for acidification and internal propeptide cleavage -- Anderson et al. To determine the requirements for the activation of furin, a series of epitope-tagged furin constructs was used (Figure 1). Molloy, S.S., Thomas, L., VanSlyke, J.K., Stenberg, P.E. and Thomas, G. Intracellular trafficking and activation of the furin proprotein convertase: localization to the TGN and recycling from the cell surface. embojournal.npgjournals.com /cgi/content/full/16/7/1508   (7565 words)

Pezzilli R, et al. Mechanisms Involved in the Onset of Post-ERCP Pancreatitis. JOP. J Pancreas (Online) 2002; ... As the lysosomal enzyme cathepsin B is known to be capable of activating trypsinogen [11] and trypsin can activate the remaining digestive enzyme zymogens, the co-localization phenomenon could result in intravacuolar digestive enzyme activation (Figure 2). Direct trauma from endoscopy rarely causes pancreatitis [12]; cannulation trauma to the papilla is the most common cause of sphincter of Oddi spasm [13] and/or an edema of the papilla, thus creating an obstacle to the flow of pancreatic juice, and subsequently determines an acute pancreatic inflammation [14]. According to the reflux pathogenesis of acute pancreatitis [36, 37], the amount of activated intestinal enzymes carried into the pancreatic ductal system by ERCP maneuvers is unknown. www.joplink.net /prev/200211/03.html   (3098 words)

2EAT: Enzyme Activation Trial.2   (Site not responding. Last check: 2007-11-04) These enzymes are frequently called metabolizing enzymes and we are interested in a specific group called glutathione-S-transferases or GST for short. In 2EAT: Enzyme Activation Trial.2, we are interested in learning how the interplay of genes and diet - in particular, a diet rich in vegetables - may affect the function of these enzymes. The activity of these enzymes will be monitored by looking at levels in blood and at the breakdown caffeine. www.fhcrc.org /phs/2eat   (187 words)

Activation of Neutrophil Collagenase in Periodontitis -- Romanelli et al. 67 (5): 2319 -- Infection and Immunity whereas the latent enzyme is predominant in gingivitis. Naturally activated collagenase obtained from a neutrophil preparation and a GCF sample from a periodontitis patient were electrophoresed on 10% SDS gels. Activation of latent collagenase of human leukocytes and gingival fluid by bacterial plaque. iai.asm.org /cgi/content/full/67/5/2319   (6232 words)

A Role in Enzyme Activation for the N-terminal Leader Sequence in Calmodulin -- Persechini et al. 271 (32): 19279 -- ... A Role in Enzyme Activation for the N-terminal Leader Sequence in Calmodulin Calmodulindependent activation of cerebellar nitric oxide synthase activity is not affected by this deletion. Based on enzyme kinetic analyses, deletions in the leader sequence have little or no effect on the apparent affinities of calmodulin for the synthase or the two kinases. www.jbc.org /cgi/content/abstract/271/32/19279   (553 words)

Guanylate cyclase from bovine lung. Evidence that enzyme activation by phenylhydrazine is mediated by iron-phenyl ... Evidence that enzyme activation by phenylhydrazine is mediated by iron-phenyl hemoprotein complexes -- Ignarro et al. Evidence that enzyme activation by phenylhydrazine is mediated by iron-phenyl hemoprotein complexes enzyme activation by NO and NaN3 was inhibited by oxidants or KCN. www.jbc.org /cgi/content/abstract/259/9/5923   (298 words)

Enzyme activation appears key in helping internal clock tell night from day PKG-II is an enzyme, a protein that triggers biochemical reactions. Reporting in the Aug. 19 issue of the journal Neuron, the Illinois scientists say that the activation of PKG-II may be the critical control point that tells our biological clock to proceed by putting night behind and journeying into a new day. By doing so, they disrupted the key activity of this kinase enzyme that adds phosphates to proteins, a signaling mechanism necessary for information transfer in cells. www.medicalnewstoday.com /medicalnews.php?newsid=12252   (540 words)

Calcium-Calmodulin-dependent Protein Kinase II and Protein Kinase C-mediated Phosphorylation and Activation of ... Competition experiments were performed by immunodetecting enzyme from 1321N1 cells in the presence of 5 µg of the corresponding peptides (lanes 5 and 6). Cells were lysed in the presence of protease and phosphatase inhibitors and enzyme was purified by CaM-Sepharose in each condition. The procedures for lysis and enzyme purification are the same as described in the legend of Fig. www.jbc.org /cgi/content/full/274/21/14734   (5282 words)

Myogenic NOS in canine lower esophageal sphincter: enzyme activation, substrate recycling, and product actions -- ... Activation is expected to occur whenever micromolar Ca concentrations are present at the intracellular surface of the a new arginine molecule." This coinduction of the AS enzyme with Acetylcholine activates non-selective cation and chloride conductances in canine and guinea-pig tracheal myocytes. ajpcell.physiology.org /cgi/content/full/274/4/C1145   (6858 words)

A Unified Model for Signal Transduction Reactions in Cellular Membranes -- Haugh 82 (2): 591 -- Biophysical Journal The concentration of a membrane-anchored substrate is determined by the relative rates of consumption and transfer to the membrane. Both the turnover and supply of the substrate are modulated by activated receptors. The diffusion-limited collision-coupling activation mechanism in membranes was examined by Shea et al. www.biophysj.org /cgi/content/full/82/2/591   (4840 words)

Enzyme activation of molecular oxygen molecule, its sluggish reactivity and activation by enzymes are still not completely understood. The biological systems activate triplet dioxygen for controlled chemical synthesis via electron-transfer and proton transfer reduction. activation and usually EPR studies of such reactions have mostly relied on the symmetry of the g-tensor and on the magnitude of HFC constants in the analysis of the reaction center, while the quantitative structure of the g-tensor, which provides additional information on the ligands is difficult to analyse in term of structural characteristics. www.theochem.kth.se /people/vahtras/research/node16.html   (351 words)

Structure-function discrimination of the N- and C- catalytic domains of human angiotensin-converting enzyme: ... Structure-function discrimination of the N- and C- catalytic domains of human angiotensin-converting enzyme: implications for Cl- activation and peptide hydrolysis mechanisms -- Tzakos et al. The molecular mechanism of chloride activation is not readily the activated water to the scissile amide nitrogen of the substrate. peds.oupjournals.org /cgi/content/full/16/12/993   (5883 words)

Enzymes in organic media: Forms, functions and applications -- Gupta and Roy 271 (13): 2575 -- FEBS Journal   (Site not responding. Last check: 2007-11-04) Keywords: antibodies in organic solvents; enzymes in organic solvents; high activity enzyme formulations; medium engineering; microwave assisted enzymatic reactions; synthesis and modification of polymers. was that salt activation is mechanistically distinct from lyoprotection. Lindsay, J.P., Clark, D.S. and Dordick, J.S. (2002) Penicillin amidase is activated for use in nonaqueous media by lyophilizing in the presence of potassium chloride. content.febsjournal.org /cgi/content/full/271/13/2575   (5239 words)

Calcium-dependent enzyme activation and vacuole formation in the apical granular region of pancreatic acinar cells -- ...   (Site not responding. Last check: 2007-11-04) Calcium-dependent enzyme activation and vacuole formation in the apical granular region of pancreatic acinar cells -- Raraty et al. Calcium-dependent enzyme activation and vacuole formation in the apical granular region of pancreatic acinar cells enzyme activation is normally kept to a minimum, but in the often-fatal www.pnas.org /cgi/content/abstract/97/24/13126   (1128 words)

Arthritis Research & Therapy | Full text | Identification of a B cell activation specific enzyme Notably, these two types of genetic alteration in the immune system take place in a highly specialized microenvironment called the germinal center resulting in the generation of high-affinity antibodies for a given antigen during affinity maturation. However, the involved enzymes or enzymatic complexes are not identified to date. This was confirmed by measuring serum levels of IgM, IgG, and IgA in AID-/- mice at 10 weeks of age and immunizing AID-/- chimera. arthritis-research.com /paperreport/ar-2000-66856   (479 words)

Pathogenic Role of Oxidative Stress in Vascular Angiotensin-Converting Enzyme Activation in Long-Term Blockade of ...   (Site not responding. Last check: 2007-11-04) Pathogenic Role of Oxidative Stress in Vascular Angiotensin-Converting Enzyme Activation in Long-Term Blockade of Nitric Oxide Synthesis in Rats -- Usui et al. Pathogenic Role of Oxidative Stress in Vascular Angiotensin-Converting Enzyme Activation in Long-Term Blockade of Nitric Oxide Synthesis in Rats Takemoto M, Egashira K, Usui M, Numaguchi K, Tomita H, Tsutsui H, Shimokawa H, Sueishi K, Takeshita A. Important role of tissue angiotensin-converting enzyme activity in the pathogenesis of coronary vascular and myocardial structural changes induced by long-term blockade of nitric oxide synthesis in rats. hyper.ahajournals.org /cgi/content/full/34/4/546   (3237 words)

SeedQuest - Central information website for the global seed industry The formation of this cysteinyl adduct leads to a change both in the conformation of the flavin and the LOV domain itself, bringing about an unfolding of the amphipathic alpha helix. The consequence of this unfolding is activation of the kinase domain and hierarchical phosphorylation. This light-activated protein unfolding represents a unique photochemical mechanism for enzyme activation and the initiation of signal transduction. www.seedquest.com /News/releases/2004/july/9386.htm   (686 words)

Effect of Chilling on Carbon Assimilation, Enzyme Activation, and Photosynthetic Electron Transport in the Absence of ... Effect of Chilling on Carbon Assimilation, Enzyme Activation, and Photosynthetic Electron Transport in the Absence of Photoinhibition in Maize Leaves -- Kingston-Smith et al. Effect of Chilling on Carbon Assimilation, Enzyme Activation, and Photosynthetic Electron Transport in the Absence of Photoinhibition in Maize Leaves activation may compensate for decreases in maximal catalytic activity. www.plantphysiol.org /cgi/content/abstract/114/3/1039   (582 words)

Identification of the Enzyme Required for Activation of the Small Ubiquitin-like Protein SUMO-1 -- Desterro et al. 274 ... Identification of the Enzyme Required for Activation of the Small Ubiquitin-like Protein SUMO-1 -- Desterro et al. Activation of Transforming Growth Factor-{beta} Signaling by SUMO-1 Modification of Tumor Suppressor Smad4/DPC4 Activation of p53 by conjugation to the ubiquitin-like protein SUMO-1 dx.doi.org /10.1074/jbc.274.15.10618   (1625 words)

Promega Notes Abstract: Inhibition of EGF-Induced ERK1 and ERK2 Enzyme Activation in NIH3T3 Cells by in situ ... Inhibition of EGF-Induced ERK1 and ERK2 Enzyme Activation in NIH3T3 Cells by in situ Electroporation of a Nonpermeant Inhibitor Cells were subsequently stimulated with growth factors and assessed for activation of a downstream target, the extracellular signal regulated kinase (ERK1/2), by probing with highly specific antibodies. The results showed that this compound can inhibit EGF- but not PDGF-mediated ERK1/2 activation in vivo, demonstrating the specificity of this compound and the utility of the in situ electroporation approach for the study of tyrosine kinase action in intact cells. www.promega.com /pnotes/67/7201_12/7201_12.html   (202 words)

IngentaConnect Proximal Regions of the Catalytic gamma and Regulatory beta Subun...   (Site not responding. Last check: 2007-11-04) Activation of the kinase by a variety of means caused similar increases in the binding to the holoenzyme of the monoclonal antibodies against these two regions of the subunits recognized by the monoclonal antibodies are structurally coupled to each other and with the activation of phosphorylase kinase. Activation of the holoenzyme apparently involves the repositioning of the base of the catalytic domain of the www.ingentaconnect.com /content/ap/mb/1997/00000265/00000003/art00739   (336 words)

Crystal structure of calpain reveals the structural basis for Ca2+-dependent protease activity and a novel mode of ...   (Site not responding. Last check: 2007-11-04) Crystal structure of calpain reveals the structural basis for Ca2+-dependent protease activity and a novel mode of enzyme activation Crystal structure of calpain reveals the structural basis for Ca -dependent protease activity and a novel mode of enzyme activation This Ca -dependent activation mechanism is clearly distinct from those of classical proteases. dx.doi.org /10.1093/emboj/18.24.6880   (274 words)

Enzyme-Catalyzed Activation of Anticancer Prodrugs -- Rooseboom et al. 56 (1): 53 -- Pharmacological Reviews Enzyme-Catalyzed Activation of Anticancer Prodrugs -- Rooseboom et al. Discussion of Cytochrome P450 Reductase as a Prodrug-Activating Enzyme. Discussion of Cytochrome P450 as a Prodrug-Activating Enzyme. www.pharmrev.org /cgi/content/abstract/56/1/53   (463 words)

Crystal structure of calpain reveals the structural basis for Ca2+-dependent protease activity and a novel mode of ...   (Site not responding. Last check: 2007-11-04) Calpains are the only known mammalian enzymes that combine protease activity with a dependence on Ca -binding to EF-hands in one molecule. With the exception of calpain, all known cellular proteolytic enzymes are synthesized as zymogens, or inactive precursors, Khan, A.R. and James, M.N.G. Molecular mechanisms for the conversion of zymogens to active proteolytic enzymes. www.emboj.org /cgi/content/full/18/24/6880   (6788 words)

Try your search on: Qwika (all wikis)

About us   |   Why use us?   |   Reviews   |   Press   |   Contact us   Copyright © 2005-2007 www.factbites.com Usage implies agreement with terms.