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Topic: Enzyme linked immunoabsorbent assay

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	Enzyme-linked immunosorbent assay (ELISA) for determining anti-bodies against herpes simplex virus (HSV) types 1 and 2 ...
		A preferred embodiment of the method employs a variation of the enzyme-linked immunosorbent assay (ELISA) whereby monoclonal antibodies are used to purify target antigens, and test sera are absorbed with virus-infected cell extracts to remove intertypic cross-reacting antibodies.
		For assaying antibodies to HSV-2, one serum sample is mixed with an equal voume (100 ul) of HSV-1 infected cell extract diluted 1:10 with BSA-PBS, and a duplicate sample is mixed with an equal volume of uninfected Vero cell extract diluted 1:10 with BSA-PBS.
		For assaying antibodies to HSV-1, one serum sample is mixed with an equal volume (100 ul) of HSV-2 infected cell extract diluted 1:10 with BSA-PBS, and a duplicate sample is mixed with an equal volume of uninfected Vero cell extract diluted 1:10 with BSA-PBS.
	www.freepatentsonline.com /4764459.html (4574 words)

	ENZYME-LINKED IMMUNOABSORBENT ASSAY
		The enzyme-linked immunoabsorbent assay is serologic test used as a general screening tool for the detection of antibodies to the HIV virus.
		ELISA technology links an a measurable enzyme to either an antigen or antibody.
		In this way, it can then measure the presence of an antibody, or an antigen in the bloodstream.
	www.medhelp.org /glossary/new/gls_1703.htm (99 words)

	Food Safety Research Information Office: A Focus on Bovine Spongiform Encephalopathy
		Develop assays for identification of transmission pathways through direct animal contact and environmental contamination.
		Correlate prion isoforms with ruminant and cervid susceptibility.
		Evaluate capillary immunoelectrophoresis assay (CIE) for Creutzfeld-jakob Disease in humans using scrapie as a model and validate this CIE assay for its sensitivity to detecting abnormal PrP in blood.
	fsrio.nal.usda.gov /document_fsheet.php?product_id=169 (4562 words)

	AEGiS-11IAC: HIV-specific IgA antibodies in vertically exposed babies.
		STUDY DESIGN: A modified IgA antibody-capture enzyme -linked immunoabsorbent assay was used to detect HIV-specific IgA antibodies in serial plasma samples collected from 65 infants born to HIV-infected mothers in the Bahamas and in Toronto.
		The specificity of this assay when evaluated for babies born to seronegative women is 100%.
		Loss of these antibodies in 100% of uninfected babies after 8 months of age and its continued presence in infected babies makes this test useful in the diagnosis of HIV infection in areas where other serological tests are not reliable.
	www.aegis.com /conferences/iac/1996/WeA3012.html (375 words)

	Cecil Textbook of Medicine : />
		For example, when the D-dimer level is 500 μg/L or greater, the sensitivity for PE may be as high as 96 to 98%, but the specificity is much lower.
		A number of D-dimer assays are available, and the sensitivity and specificity of these assays vary.
		Troponin elevation is specific for cardiac myocyte damage, and the right ventricle appears to be the source of the enzyme elevation in acute PE.
	www.merckmedicus.com /ppdocs/us/common/cecils/chapters/094_007.htm (1679 words)

	Western blotting is useful in the salivary diagnosis of Helicobacter pylori infection -- Ballam et al. 53 (4): 314 -- ... (Site not responding. Last check: 2007-07-25)
		Figure 3 Sensitivity and specificity (%) of saliva enzyme linked immunoabsorbent assay (ELISA) against serum ELISA by cut off point in 665 subjects with samples for both.
		Lane 1, positive serum control; lanes 2–4, serum enzyme linked immunoabsorbent assay (ELISA) negative specimens; lanes 5–11, serum ELISA positive specimens; lanes 12–25, serum ELISA negative specimens.
		Detection of Helicobacter pylori in stool specimens by PCR and antigen enzyme immunoassay.
	jcp.bmjjournals.com /cgi/content/full/53/4/314 (2330 words)

	HBPD INT
		Antigens and antibodies of HBV were determined by enzyme immune assay.
		Antibody to HCV IgG was detected by enzyme-linked immunoabsorbent assay.
		Antigens and antibodies of HBV were determined by enzyme immune assay (Kehua Biotech Co., Ltd, Shanghai, China).
	www.hbpdint.com /text.asp?id=638 (744 words)

	Fetal Fibronectin Enzyme Immunoassay
		(2) It is still unknown whether the use of the FFN assay results in a decrease in the rate of morbidity and mortality related to preterm birth.
		However, observational studies in women with symptoms suggestive of PTL report that the positive predictive value of the FFN assay for preterm birth ranged from 0.45 to 0.81, while the negative predictive value for preterm birth within 14 days of the test was 0.95 to 0.99.
		Although the FFN assay is a risk predictor of preterm delivery among asymptomatic women, there is no proven effective treatment for asymptomatic women at risk of preterm birth that is known to improve maternal or fetal outcomes.
	www.regence.com /trgmedpol/maternity/mat10.html (2299 words)

	DEFRA Science
		Currently there is no internationally approved enzyme linked immunoabsorbent assay (ELISA) which can be used to test companion animal sera for the presence of rabies virus specific antibodies in response to vaccination.
		the main obstacle to the development of such an assay is the production of an appropriate cost-effective antigen which does not require culture of live rabies virus.
		The prototype assay will be validated against a large panel of post-vaccination sera and against the fluorescent antibody virus neutralisation (FAVN) test, the current Office International des Epizooties (OIE) gold standard.
	www2.defra.gov.uk /research/Project_Data/More.asp?I=SE0416&M=CFO&V=VLA (268 words)

	Archivos de Bronconeumología (English Version): Texto completo: Usefulness of Fast ELISA Determination of D-Dimer ... (Site not responding. Last check: 2007-07-25)
		The traditional latex agglutination assay has fallen into disuse because of its lower sensitivity compared to other methods; turbidimetric techniques and enzyme-linked immunoabsorbent assay (ELISA) techniques are the most sensitive, whereas the usefulness of the SimpliRED assay is still under debate.
		A prospective study of consecutive patients attended between September 2002 and July 2003 in the Emergency Room of the Hospital de la Princesa in Madrid with clinical suspicion of pulmonary embolism was conducted.
		Semiquantitative latex agglutination assays are rapid, simple, and cheaper, but sensitivity is limited (83%) and the negative predictive value for pulmonary embolism is 90 %.
	db.doyma.es /cgi-bin/wdbcgi.exe/doyma/mrevista.fulltext?pident=13079311 (5863 words)

	An Improved Enzyme-Linked Immunoabsorbent Assay Protocol for the Detection of Small Lytic Peptides in Transgenic ...
		An Improved Enzyme-Linked Immunoabsorbent Assay Protocol for the Detection of Small Lytic Peptides in Transgenic Grapevines (Vitis Vinifera)
		An enzyme-linked immunoabsorbent assay (ELISA) protocol was developed for the detection of small lytic peptides in transgenic grapevines (V.
		The protocol requires a high concentration of protease inhibitor in the extraction buffer; the use of antiserum cross-absorbed with control tissue, an increased concentration of blocking reagents in the antiserum buffer, and performing all coating and/or binding processes at 37°C while reducing the time period for each step to 1 h.
	pubs.nrc-cnrc.gc.ca /ispmb/r01-046.html (164 words)

	HIV & AIDS Jargon Part II (HIV test) - Aids / HIV
		ELISA (Enzyme-linked immunoabsorbent assay): This is the blood test that is done to detect HIV antibodies in order to determine if you have been exposed to HIV.
		Reverse transcriptase: This is an enzyme produced by HIV that enables it to change its RNA to DNA.
		Without this enzyme, HIV would not be able to replicate.
	www.bellaonline.com /ArticlesP/art40387.asp (352 words)

	Elisa: Theory and Practice - PowerBookSearch!
		Introduces to scientists at all levels of expertise the principles of the most commonly used assay technique, known as Enzyme Linked Immunoabsorbent Assay.
		Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
		Mastery of the book's contents will allow you to fully appreciate exactly how and why assays function, as well as to efficiently develop assays suitable to your own research that are both rapid and accurate.
	www.powerbooksearch.com /booksearch0896032795.html (935 words)

	Screening for inhibitors of HIV gp120-CD4 binding using an enzyme-linked immunoabsorbent assay. (Site not responding. Last check: 2007-07-25)
		Screening for inhibitors of HIV gp120-CD4 binding using an enzyme-linked immunoabsorbent assay.
		The state of the art may have changed since the publication date.
		The results demonstrate that this assay system can be used to measure the activity of inhibitors of CD4-gp120 binding, and to determine the mechanism of action of those inhibitors.
	www.aegis.com /aidsline/1993/oct/M93A0118.html (453 words)

	Enzyme-linked immunoabsorbent assay-detected p53 protein accumulation: a prognostic factor in a large breast cancer ... (Site not responding. Last check: 2007-07-25)
		Enzyme-linked immunoabsorbent assay-detected p53 protein accumulation: a prognostic factor in a large breast cancer cohort -- Levesque et al.
		Enzyme-linked immunoabsorbent assay-detected p53 protein accumulation: a prognostic factor in a large breast cancer cohort
		were assayed for p53 protein by an ELISA that used both DO-1 monoclonal and
	www.jco.org /cgi/content/abstract/16/8/2641 (569 words)

	Hepatitis GB virus C/ Hepatitis G virus:
		According to reported data, there are no conclusive evidences for causal link between this virus and either acute or chronic liver disease.
		Recombinant GBV-C/HGV envelope protein E2 was used as antigen in an enzyme-linked immunoabsorbent assay (ELISA).
		It was noticed that the clearance of viremia in selected group of patients was followed by the appearance of anti E2 antibody.
	www.yuga.org.yu /archiv/gastroyu/jan2001/article5.htm (7888 words)

	Competitive Enterprise Institute
		Maybe we should be skeptical of cute-sounding acronyms, because in the real world of agriculture and food, mischievous groups can misuse a technology called ELISA to wreak similar havoc — halting commerce, closing ports and stirring up international disputes that often pit country against country.
		ELISA (which stands for enzyme-linked immunoabsorbent assay) is a diagnostic test that can detect very low levels of certain proteins in very large batches of food or commodity grains.
		Environmental extremist groups like it because ELISA can detect biotech material in conventional grain shipments.
	www.cei.org /gencon/019,03082.cfm (1487 words)

	Energy Citations Database (ECD) - Energy and Energy-Related Bibliographic Citations
		Energy Citations Database (ECD) Document #6781878 - An enzyme-linked immunoabsorbent assay for estimating red cell survival of transfused red cells-validation using CR-51 labeling
		Availability information may be found in the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or via the "Full-text Availability" link.
		An enzyme-linked immunoabsorbent assay for estimating red cell survival of transfused red cells-validation using CR-51 labeling
	www.osti.gov /energycitations/product.biblio.jsp?osti_id=6781878 (167 words)

	EXOPOL: Circular 105 (Site not responding. Last check: 2007-07-25)
		using Assurance Salmonella EIA (an ELISA, or enzyme-linked immunoabsorbent assay test, obtained from BioControl Systems, Inc., of Bothell, Washington, and performed by an Immunotech automated ELISA machine from Italy).
		As control strains, Salmonella luciana and Citrobacter species were obtained from the bacterial-culture collection of the Oregon Department of Agriculture (ODA) Laboratory Services Division.
		Feldsine, P.T., Falbo-Nelson, M.T., and Hustead, D.L. Polyclonal enzyme immunoassay method for detection of motile and non-motile Salmonella in foods: Comparative study.
	www.exopol.com /general/circulares/105circ.html (4400 words)

	I A FORSYTH et al.: Monoclonal antibodies to ovine PL (Journal of Endocrinology)
		By competitive enzyme-linked immunoabsorbent assay (ELISA), confirmed by two-site ELISA, the antibodies were shown to recognize 6 antigenic determinants on the ovine PL molecule, two of which overlap.
		One antigenic determinant (designated 1) was shared by other members of the prolactin/growth hormone (GH)/PL family in ruminants, humans and rodents.
		In the local in vivo pigeon crop sac assay, mAbs directed against determinants 3 and 6 enhanced the biological activity of ovine PL.
	journals.endocrinology.org /joe/165/joe1650435.htm (210 words)

	Production of Interleukin 13 by Alveolar Macrophages from Normal and Fibrotic Lung -- Hancock et al. 18 (1): 60 -- ...
		All positive samples were readily confirmed by agarose gel examination.
		to recombinant standards run in parallel with each batch of assays.
		Overlapping and enzyme-specific contributions of matrix metalloproteinases-9 and -12 in IL-13-induced inflammation and remodeling
	ajrcmb.atsjournals.org /cgi/content/full/18/1/60 (4231 words)

	Dream magazine online
		A welcome letter from President and CEO James Mandell, MD Other issues
		Researcher Marsha Moses, PhD, looks at a urinary cancer biomarker ELISA (enzyme-linked immunoabsorbent assay).
		In her experiments, each well is filled with an antibody to a given protein the Moses Lab has shown predicts cancer status and stage.
	www.childrenshospital.org /dream/DreamRsch2005 (228 words)

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