
	Where results make sense

Topic: Episome

In the News (Thu 8 Jan 09)

Rod Blagojevich

Phil Schiller

Gaza City

Jermain Defoe

Lindsay Lohan

Bill Richardson

Kelly Preston

Leon Panetta

Mitchell Johnson

Darren Sproles

	Highbeam Encyclopedia - Search Results for episome
		Episomes may be attached to the bacterial cell membrane or become part of the chromosome.
		Cells with episomes act like males during conjugation, a mating process in...
		Common antiviral drugs do not inhibit episomal virus DNA synthesis, indicating that the latent form of viral DNA is replicated...
	www.encyclopedia.com /SearchResults.aspx?Q=episome (750 words)

	EBV-based Episomes
		To establish as stable episomes, it is required that the Epstein Barr virus Nuclear Antigen (EBNA) binds to the EBV origin of replication and nuclear retention sequences.
		The episome is thereby maintained at 5 to 20 copies per cell for up to two to three months, given a puromycin-resistance gene resident and selected for on the plasmid.
		The episome is thereby maintained at 5 to 20 copies per cell for up to two to three months, as long as some drug marker is resident and selected for on the plasmid.
	www.stanford.edu /group/nolan/retroviral_systems/phx_episomals.html (1664 words)

	episome — FactMonster.com
		As autonomous units they destroy host cells, and as segments integrated into a chromosome they multiply in cell division and are transferred to daughter cells.
		Episomes called sex factors determine whether chromosome material will be transferred from one bacterium to another.
		Other episomes carry genes that make bacteria resistant to the inhibitory action of antibiotics.
	www.factmonster.com /ce6/sci/A0817506.html (176 words)

	Episome vectors offer fresh angle to gene therapy
		A European research team is seeking to overcome the early setbacks suffered by the promising new field of 'gene therapy' by developing a revolutionary delivery technique, known as 'episome vectors'.
		"Episomes are DNA elements that do not combine themselves with the genetic substance of the host DNA.
		The information contained within the episome is jointly read with that of the chromosome and, together, they multiply every time cell division occurs.
	www.news-medical.net /?id=6696 (682 words)

	EUROPA - Research - Headlines - Episome vectors offer fresh angle to gene therapy
		A European research team is seeking to overcome the early setbacks suffered by the promising new field of ‘gene therapy’ by developing a revolutionary delivery technique, known as ‘episome vectors’.
		“Episomes are DNA elements that do not combine themselves with the genetic substance of the host DNA.
		The information contained within the episome is jointly read with that of the chromosome and, together, they multiply every time cell division occurs.
	europa.eu.int /comm/research/headlines/news/article_04_12_02_en.html (597 words)

	Chromosome Binding Site of Latency-Associated Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus Is Essential ...
		the KSHV oriP-based plasmid as an episome in BJAB cells (1).
		The extracted plasmids and the Z6-13 plasmid were cut with EcoRV and electrophoresed on a 0.7% agarose gel, and the separated plasmid fragments were stained with 0.5 µg of ethidium bromide/ml.
		with mitotic chromosomes and inhibited the long-term episome
	jvi.asm.org /cgi/content/full/76/24/12917 (4732 words)

	The latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus interacts preferentially with the ...
		The latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus interacts preferentially with the terminal repeats of the genome in vivo and this complex is sufficient for episomal DNA replication -- Fejér et al.
		The positions of bands corresponding to transfected episome replicating plasmid and the resident KSHV genome are indicated.
		Episomal DNA was extracted from cell cultures by the method of Hirt (1967)
	vir.sgmjournals.org /cgi/content/full/84/6/1451 (5919 words)

	Inefficient establishment of KSHV latency suggests an additional role for continued lytic replication in Kaposi sarcoma ...
		Episomes were detected with a probe specific for the GFP cassette.
		The position of supercoiled episomes (upper band) and linear as well as nicked and partially degraded KSHV DNA (lower band) are marked by arrows labeled sup and lin, respectively.
		Episomal DNA was isolated at the time points indicated (in days) above the lanes by Hirt extraction and subjected to PCR amplification of the GFP cassette as described in Methods.
	www.jci.org /cgi/content/full/113/1/124 (8103 words)

	Mechanisms of MLL gene rearrangement: site-specific DNA cleavage within the breakpoint cluster region is independent of ... (Site not responding. Last check: 2007-10-09)
		The percent of endogenous (End) and episomal (Epi) alleles cleaved (estimated by dividing the intensity of the cleaved allele by the intensity of cleaved allele plus germline allele; all intensities adjusted for background) is indicated below the lane.
		The episomal band from C328A is not visualized by indirect end-labeling with the 0.2HB probe since there are only 66 bp of complementary exon 9 sequence contained on the 0.2HB probe, and the C328A clone undergoes minimal cleavage.
		SAR assays of the germline and episomal MLL bcr.
	hmg.oxfordjournals.org /cgi/content/full/10/22/2481 (7144 words)

	[No title]
		First, the infectious transformation of F- by F+ cells to become F+ is a result of rolling circle replication of the F episome and the transport of the linear copy of the episome through the pili to the F- recipient cell during conjugation.
		The F episomes confers the ability to form pili and conjugation and serves as a point of origin for rolling circle replication.
		Sometimes the episome carries with it a gene that was adjacent to the point of insertion.
	sun.science.wayne.edu /~bio307/Bacterial_Mapping.html (1504 words)

	Molecular Genetics of Prokaryotes Problem Set (Site not responding. Last check: 2007-10-09)
		The lac operon sequences on the bacterial chromosome and F' episome are shown in the diagram:
		When the inducer, either lactose or allolactose is present, the inducer binds to the repressor causing an allosteric switch to a conformation that does not bind the operator DNA sequence.
		In summary, all normal mechanisms for regulation of the lac operon expression of both the chromosomal and episomal copies of the operon are present.
	www.biology.arizona.edu /molecular_bio/problem_sets/mol_genetics_of_prokaryotes/07t.html (261 words)

	History
		"Episome" to describe genetic elements such as F, colicine, and phage lambda which can exist both in association with the chromosome and independent of it.
		"The Campbell Model" as it came to be known, explained the reversible association of some episomes with the chromosome, the inversion of the genetic map of lambda bacteriophage upon lysogeny as recently reported in 1960 by E. Calef and G.
		The apparently crucial insight of Campbell was that the episome must exist as a physical circular DNA structure.
	histmicro.yale.edu /history1.htm (3281 words)

	Research project An orthogonal episome: an artificial genetic system based on ...
		An orthogonal episome: an artificial genetic system based on novel type of nucleic acids.
		Our aim is to design and build artificial genetic systems based on alternative nucleic acid architectures and "invade" biological information processing with an orthologous autonomously replicating episome (which we nage the "orthosome").
		We propose to build the orthosome from sugar modified oligonucleotides HNA (hexitol nucleic acids) and CeNA (cyclohexene nucleic acids) because of their conformational analogy, helix forming properties and resistance to enzymatic degradation.
	www.kuleuven.ac.be /research/researchdatabase/project/3M06/3M060323.htm (108 words)

	Adaptive Mutation: Has the Unicorn Landed? -- Foster 148 (4): 1453 -- Genetics
		the mutational process is not confined to the episome.
		mechanism on the episome may be important in the evolution of
		, P. and J., 1995b Conjugation is not required for adaptive reversion of an episomal frameshift mutation in Escherichia coli.
	www.genetics.org /cgi/content/full/148/4/1453 (3725 words)

	Detection of High-Risk Cervical Intraepithelial Neoplasia and Cervical Cancer by Amplification of Transcripts Derived ...
		All of the amplimers that are visible in the agarose gel hybridized with the E7-specific oligonucleotide, thus, confirming the specificity of the assay.
		genomes in the absence of episomal HPV genomes.
		Kristiansen E., Jenkins A., Holm R. Coexistence of episomal and integrated HPV16 DNA in squamous cell carcinoma of the cervix.
	cancerres.aacrjournals.org /cgi/content/full/59/24/6132 (4607 words)

	Bacterial Recombination (Site not responding. Last check: 2007-10-09)
		The excision is sometimes sloppy, however, and the F factor takes a small segment of the bacterial chromosome with it.
		F' cells are able to initiate conjugation with F- cells because of the presence of the F factor.
		This cell, however, also has a complete chromosome, so it will be diploid for the segment of chromosomal DNA on the episome.
	www.emunix.emich.edu /~rwinning/genetics/bactrec4.htm (277 words)

	In Vivo Activity of Murine De Novo Methyltransferases, Dnmt3a and Dnmt3b -- Hsieh 19 (12): 8211 -- Molecular and ...
		episome or the methylation-free region of a patch-methylated episome
		It is possible that the de novo methylation of the episome observed was an indirect effect of Dnmt3a or Dnmt3b because both
		Targets of Dnmt3a and Dnmt3b de novo methylation activity on the episome are not random.
	mcb.asm.org /cgi/content/full/19/12/8211 (6760 words)

	Tramadol, buy tramadol, antibiotics, Weight Loss drugs, weight loss herbs, weight loss herbal formula, hair loss, hair ... (Site not responding. Last check: 2007-10-09)
		The latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus interacts preferentially with the terminal repeats of the genome in vivo and this complex is sufficient for episomal DNA replication.
		The genome of Kaposi's sarcoma-associated herpesvirus (KSHV) persists in latently infected cells as a circular episome.
		These results also suggest that the LANA expression plasmids reported here could be utilized as episomal vectors in a manner similar to Epstein-Barr virus-based vectors.
	drugs-online-usa.com /ref-sarcoma/sarcoma-research-abs6.425.html (1881 words)

	Definition of episome - Merriam-Webster Online Dictionary
		Learn more about "episome" and related topics at Britannica.com
		Find more about "episome" instantly with Live Search
		See a map of "episome" in the Visual Thesaurus
	www.m-w.com /dictionary/episome (51 words)

	Molecular Genetics of Prokaryotes Problem Set (Site not responding. Last check: 2007-10-09)
		During conjugation, a copy of the F' factor DNA is transferred from the male to the female.
		In this experiment, the lac Z gene, encoding ß-galactosidase and the regulatory sequences of the operator are transferred with the F' factor.
		Transfer of an F' factor episome is a specialized type of conjugation known as sexduction.
	www.biology.arizona.edu /molecular_bio/problem_sets/mol_genetics_of_prokaryotes/05t.html (216 words)

	Chromosomal DNA demethylation specified by protein binding -- Lin and Hsieh 2 (2): 108 -- EMBO Reports (Site not responding. Last check: 2007-10-09)
		of the lacO sequence as observed on the stable episome (Lin et al.
		the phenomenon observed on the stable episome is representative
		Sugden, B., Marsh, K. and Yates, J. (1985) A vector that replicates as a plasmid and can be efficiently selected in B-lymphoblasts transformed by Epstein-Barr virus.
	emboreports.npgjournals.com /cgi/content/full/2/2/108 (3411 words)

	Birgid Schlindwein's Hypermedia Glossary Of Genetic Terms - Search results (Site not responding. Last check: 2007-10-09)
		The term introduced by Jacob and Wollman (1958b) for a particle which may at some time exist attached to and apparently integrated with the hereditary material of a cell, and which may at other times occur free from such attachment.
		When attached, episomes replicate along with the hereditary material, but when free they usually replicate independently of it and more rapidly.
		Examples of episomes are many bacterial viruses and the male sex factor in Escherichia coli.
	hal.weihenstephan.de /genglos/asp/genreq.asp?nr=69 (145 words)

	US PATENT SUBCLASS 435 / 476-- .~.~ The polynucleotide is a plasmid or episome
		> Plasmid or episome confers the ability to utilize directly a compound which a wild type microorganism is unable to utilize
		(under subclass 471) Processes wherein the nucleic acid is a plasmid or episome and therefore capable of autonomous replication in the microorganism cell.
		The nucleic acid is either circular or linear in its replicating form and is circular, linear, or a linearized circle when introduced into the microbial cell.
	www.patentec.com /data/class/defs/435/476.html (445 words)

	Induction of a DNA Nickase in the Presence of Its Target Site Stimulates Adaptive Mutation in Escherichia coli -- ...
		FC40, the revertible strain, and FC29, the nonrevertible scavenger
		RecA and RecBCD catalyze invasion of the 3' end into the homologous duplex of the sister chromosome (or another episome.) (C) A "restart" replication complex is assembled, consisting of the primosome proteins, including PriA (9) and either DNA polymerase II (48) or IV (16).
		Conjugation is not required for adaptive reversion of an episomal frameshift mutation in Escherichia coli.
	jb.asm.org /cgi/content/full/184/20/5599 (5789 words)

	Gammaherpesviruses and "Hit-and-Run" Oncogenesis -- Ambinder 156 (1): 1 -- American Journal of Pathology
		retain their episomes and thus have a growth advantage.
		episome unnecessary for survival, and in patients with relatively
		Srinivas SK, Sample JT, Sixbey JW: Spontaneous loss of viral episomes accompanying Epstein-Barr virus reactivation in a Burkitt’s lymphoma cell line.
	ajp.amjpathol.org /cgi/content/full/156/1/1 (2437 words)

	Eukaryotic cells stably expressing genes from multiple transfected episomes (US5976807)
		A method is described for producing recombinant eukaryotic cell lines expressing multiple proteins of interest.
		Eukaryotic host cells are transfected with (a) a first episome which contains an EBV origin of replication and a first gene encoding a protein of interest; and (b) a second episome containing an EBV origin of replication and a second gene encoding a protein of interest.
		The cells are grown under conditions wherein the episomes express the first and second genes.
	www.delphion.com /details?pn=US05976807__ (234 words)

	KENNETH M. KAYE, Department of Medicine (Site not responding. Last check: 2007-10-09)
		The latency-associated nuclear antigen (LANA) mediates KSHV episome persistence by permitting viral DNA replication and then tethering KSHV episomes to mitotic chromosomes to ensure their efficient segregation to progeny nuclei.
		The Kaposi’s sarcoma-associated herpesvirus latency-associated nuclear antigen 1 N-terminus is essential for chromosome association, DNA replication, and episome persistence.
		KSHV LANA1 binds DNA as an oligomer and residues N-terminal to the oligomerization domain are essential for DNA binding, DNA replication, and episome persistence.
	www.hms.harvard.edu /dms/virology/vir_kaye.html (319 words)

	ORF73 of murine herpesvirus-68 is critical for the establishment and maintenance of latency -- Fowler et al. 84 (12): ...
		of latent gammaherpesviral genomes in dividing cells are episome
		Bridgeman, A., Stevenson, P. G., Simas, J. and Efstathiou, S. A secreted chemokine binding protein encoded by murine gammaherpesvirus-68 is necessary for the establishment of a normal viral load.
		Collins, C. and Medveczky, P. Genetic requirements for the episomal maintenance of oncogenic herpesvirus genomes.
	vir.sgmjournals.org /cgi/content/full/84/12/3405 (6201 words)

	Chromatin Remodeling by Transcriptional Activation Domains in a Yeast Episome -- Stafford and Morse 272 (17): 11526 -- ...
		Chromatin Remodeling by Transcriptional Activation Domains in a Yeast Episome -- Stafford and Morse 272 (17): 11526 -- Journal of Biological Chemistry
		Chromatin Remodeling by Transcriptional Activation Domains in a Yeast Episome
		in a yeast (Saccharomyces cerevisiae) episome outside of the context
	www.jbc.org /cgi/content/abstract/272/17/11526 (617 words)

Try your search on: Qwika (all wikis)