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Esterase - Wikipedia, the free encyclopedia An esterase is a hydrolase enzyme that splits esters into an acid and an alcohol in a chemical reaction with water called hydrolysis. A wide range of different esterases exist that differ in their substrate specificity, their protein structure, and their biological function. Esterases are classified as EC 3.1 in the EC number classification of enzymes. en.wikipedia.org /wiki/Esterase   (281 words)

Use of sulfated polysaccharides to inhibit pancreatic cholesterol esterase - United States Patent 5,017,565 The present invention is directed to a method for decreasing intestinal absorption of cholesterol and fatty acid by inhibiting human pancreatic cholesterol esterase, a key enzyme involved in mediating absorption, by orally administering sulfated polysaccharides in an amount effective for inhibiting cholesterol esterase. This compound inhibited cholesterol esterase with an IC.sub.50 of 0.06.mu.g/ml or 0.025 nM. This sulfated pectin inhibited cholesterol esterase with an IC.sub.50 of 0.04.mu.g/ml or 2 nM. xrint.com /patents/us/5017565   (3609 words)

Characterization and Heterologous Gene Expression of a Novel Esterase from Lactobacillus casei CL96 -- Choi et al. 70 ... Cloning and nucleotide sequence of an esterase gene from Pseudomonas fluorescens and expression of the gene in Escherichia coli. Intracellular esterase from Lactobacillus casei LILA: nucleotide sequencing, purification, and characterization. Isolation and characterization of a tributyrin esterase from Lactobacillus plantarum 2739. aem.asm.org /cgi/content/full/70/6/3213   (4810 words)

Title of Invention: Esterase genes, esterase, recom binant plasmids and transformants containing the recombinant ... Esterase produced by the microorganism is less heat stable and is inactivated in several hours, for example, at 45.degree. In addition, the DNA fragment of the present invention may be obtained by cloning a desirable esterase gene derived from the chromosome of a microorganism or by synthesizing a desirable sequence by a DNA synthesizer. The novel esterase of the present invention may be obtained from the culture of the transformant of the present invention by the method known in the art: Enzyme endogenouly produced in the transformant is extracted by any method in the art to give bacterial-cell free extract. www.nal.usda.gov /bic/Biotech_Patents/1994patents/05308765.html   (4588 words)

Hormonally sensitive esterase activity in the mouse uterus results from uptake of plasma esterase. Consistent with this, hydrolysis of estradiol valerate and beta-alanine nitrophenyl ester by either uterine homogenates or purified esterase 1 was blocked by heating or by incubation with diisopropyl flurophosphate or antibody to esterase 1. Levels of the plasma esterase (esterase 1) were 50% higher in adult female mice than in adult males. Although the natural substrate for esterase 1 (or hydrolase II) is not known, both the purified enzyme and uterine homogenates were able to hydrolyze the valerate ester of estradiol, but not the stearate ester. www.arclab.org /medlineupdates/abstract_6499775.html   (342 words)

Identification of a Coronavirus Hemagglutinin-Esterase with a Substrate Specificity Different from Those of Influenza C ... 60% identity to the corresponding esterase of bovine coronavirus. A total of 2.5 mU of purified PV or influenza C/JJ/50 virus was incubated with BSM (12.5 mg/ml) at 37°C. At the times indicated, the incubation was stopped by heating at 95°C, and the free acetate content was determined with a commercial test kit (Boehringer Mannheim). The glycoprotein of influenza C virus is the hemagglutinin, esterase and fusion factor. jvi.asm.org /cgi/content/full/73/5/3737   (4425 words)

Complement C1 Esterase Inhibitor, Serum C1 esterase inhibitor is decreased in hereditary angioneurotic edema; decrease may be functional or quantitative In addition to decreased C1 esterase inhibitor in the serum of patients with hereditary angioneurotic edema, a unique polypeptide kinin is increased in plasma from C1 esterase inhibitor deficient patient during attacks of swelling. The acquired form includes nonhereditary C1 esterase deficiency; drug induced, allergic, and idiopathic forms, angioedema associated with autoimmune disease, especially with systemic lupus erythematosus and hypereosinophilia; angioedema occasionally associated with malignancy; and angioedema caused by physical stimuli. www.labcorp.com /datasets/labcorp/html/chapter/mono/sc003000.htm   (313 words)

Feruloyl Esterase Activity of the Clostridium thermocellum Cellulosome Can Be Attributed to Previously Unknown Domains ... The initial indication of the presence of feruloyl esterase in the cellulosome of C. with feruloyl esterases of Aspergillus niger and Aspergillus tubingensis Characterization of an acetyl xylan esterase from the anaerobic fungus Orpinomyces sp. jb.asm.org /cgi/content/full/182/5/1346   (3766 words)

Modified Low Density Lipoprotein Enhances the Secretion of Bile Salt-stimulated Cholesterol Esterase by Human ... in exon 2 and exon 7 of the cholesterol esterase gene. cholesterol esterase that is similar to the enzyme in pancreas. Detection of hormone-sensitive lipase mRNA (A) and bile salt-stimulated cholesterol esterase (B) in mouse peritoneal macrophages. www.jbc.org /cgi/content/full/272/45/28666   (4188 words)

ARS | Publication request: Molecular and Biochemical Diagnosis of Esterase-Mediated Pyrethroid Resistance in a Mexican ... Permethrin degradation assays and esterase activity gel assays indicated enhanced esterase-mediated metabolic detoxification in the Cz strain, while two other pyrethroid resistant strains, Co and SF, and the control susceptible strain had lower levels of permethrin degradation capability. A specific esterase, designated CzEst9, believed to be responsible for the esterase-mediated pyrethroid resistance in the Cz strain was purified, the gene encoding CzEst9 cloned and recombinant protein expressed in bacterial cells. Permethrin hydrolysis assays and esterase activity gels indicated enhanced esterase-mediated metabolic detoxification in the Cz strain, while two other pyrethroid resistant strains, Co and SF, and the control susceptible strain had lower levels of permethrin hydrolysis. www.ars.usda.gov /research/publications/publications.htm?SEQ_NO_115=148561&pf=1   (449 words)

Gene Cloning and Nucleotide Sequencing and Properties of a Cocaine Esterase from Rhodococcus sp. Strain MB1 -- Bresler ... The deduced amino acid sequence of the putative cocaine esterase encoded by cocE was aligned with the amino acid sequence of the glutaryl 7-aminocephalosporanic acid acylase from B. The amino acid sequence of a region of the cocaine esterase encoded by cocE (cocE) was aligned with the homologous regions of five cloned X-prolyl dipeptidyl aminopeptidases from Lactobacillus and Lactococcus spp. Identification of a cocaine esterase in a strain of Pseudomonas maltophilia. aem.asm.org /cgi/content/full/66/3/904   (3662 words)

Nitrites and leukocyte esterase   (Site not responding. Last check: 2007-10-29) Most species of bacteria that colonize in the urine cause nitrates, which are derived from dietary metabolites, to be converted to nitrites. A urine sample that tests positive for both nitrate and leukocyte esterase should be cultured for pathogenic bacteria. A negative nitrite and leukocyte esterase test indicates that a patient does not have a urinary tract infection. www.rnceus.com /ua/uanile.html   (288 words)

prbA, a Gene Coding for an Esterase Hydrolyzing Parabens in Enterobacter cloacae and Enterobacter gergoviae Strains -- ... The new gene prbA encodes an esterase responsible for the hydrolysis The nucleotide sequence of the esterase gene of E. An alignment of the sequences of the N termini of the esterases jb.asm.org /cgi/content/full/184/18/5011   (4432 words)

Hormone-sensitive Lipase Is a Cholesterol Esterase of the Intestinal Mucosa -- Grober et al. 278 (8): 6510 -- Journal ... Hormone-sensitive Lipase Is a Cholesterol Esterase of the Intestinal Mucosa -- Grober et al. esterase activity, which is almost twice the activity toward triacylglycerols. Here we have found that HSL contributes to lipase activity and is the major cholesterol esterase in the intestinal mucosa. www.jbc.org /cgi/content/full/278/8/6510   (4357 words)

The Hemagglutinin-Esterase of Mouse Hepatitis Virus Strain S Is a Sialate-4-O-Acetylesterase -- Regl et al. 73 (6): ... C virus similar to data obtained with BCV esterase (36). Influenza C virus HE, but not MHV-S esterase, is able to hydrolyze acetate esters at the glycerol side chain of sialic acids. Modification of sialic acids by 9-O-acetylation is detected in human leucocytes using the lectin property of influenza C virus. jvi.asm.org /cgi/content/full/73/6/4721   (4223 words)

C1 ESTERASE INHIBITOR DEFICIENCY: Contact a Family - for families with disabled children: information on rare syndromes ... C1 esterase inhibitor deficiency causes intermittent angioedema (swelling of face and throat) and dermal swellings. If swelling occurs in the throat, the C1 esterase inhibitor deficiency is life threatening and patients should attend hospital immediately for C1 inhibitor infusion. C1 esterase inhibitor deficiency attacks can often be mistaken for anaphylaxis, but adrenaline, antihistamines and steroids are ineffective. www.cafamily.org.uk /Direct/c08.html   (619 words)

Spontaneous regression of acquired C1 esterase inhibitor deficiency associated with splenic marginal zone lymphoma ... Spontaneous regression of acquired C1 esterase inhibitor deficiency associated with splenic marginal zone lymphoma presenting with recurrent angio-oedema -- Phanish et al. Spontaneous regression of acquired C1 esterase inhibitor deficiency associated with splenic marginal zone lymphoma presenting with recurrent angio-oedema of the C1 esterase inhibitor deficiency and angio-oedema, as jcp.bmjjournals.com /cgi/content/full/55/10/789   (1100 words)

Intracellular Esterase from Lactobacillus casei LILA: Nucleotide Sequencing, Purification, and Characterization -- ... Intracellular Esterase from Lactobacillus casei LILA: Nucleotide Sequencing, Purification, and Characterization -- Fenster et al. Esterase activity was detected using a pour plate enzyme assay esters by esterases and lipases is favored by elevated a jds.fass.org /cgi/content/full/86/4/1118   (5338 words)

The acetyl xylan esterase of Bacillus pumilus belongs to a family of esterases with broad substrate specificity -- ... The acetyl xylan esterase of Bacillus pumilus belongs to a family of esterases with broad substrate specificity -- Degrassi et al. Biely, P., MacKenzie, C. R., Puls, J. and Schneider, H. Cooperativity of esterases and xylanases in the enzymatic degradation of acetyl xylan. Poutanen, K. and Sundberg, M. An acetyl esterase of Trichoderma reesei and its role in the hydrolysis of acetyl xylans. mic.sgmjournals.org /cgi/content/full/146/7/1585   (2843 words)

Evaluation of Leukocyte Esterase Reagent Strips for Rapid Diagnosis of Pyogenic Meningitis The detection of polymorphonuclear leukocytes in the CSF is a fairly reliable indicator of pyogenic meningitis but facilities required for its detection are not always available in rural hospitals in developing countries. The urine dipstick leukocyte esterase test uses the ability of esterase enzyme present in the polymorphonuclear leucocytes to convert a cetates to phenols. An indigenously developed leukocyte esterase test, especially if used along with the Pandy test, may have a role in the diagnosis of meningitis. www.indianpediatrics.net /sep20.htm   (642 words)

FDA Grants Orphan Drug Designation To Human C1 Esterase Inhibitor   (Site not responding. Last check: 2007-10-29) Hereditary angioedema (HAE) is an autosomal dominant disorder that results in a deficiency of functional human C1 Esterase inhibitor. In certain European countries, human C1 Esterase inhibitor produced from pooled human plasma is used as an effective therapy. A recombinant version of the human C1 Esterase inhibitor, produced in the milk of transgenic animals, will circumvent potential supply issues and constitutes a product that by definition is free of human viruses. www.pslgroup.com /dg/eede2.htm   (352 words)

Esterase antibodies from RDI Divison of Fitzgerald Industries Intl Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Rabbit Serum as well as purified and partially purified esterase (porcine liver). Cross reactivity against esterase from other tissues and species may occur but have not been specifically determined. Not for use in or on humans or animals or for diagnostics. www.researchd.com /miscabs/esterase.htm   (429 words)

A Stability Study of the Esterase D Isoenzymes   (Site not responding. Last check: 2007-10-29) Evidence of variations in human red cell esterase was first presented by Tashian [1-3]. With starch gel, borate buffer electrophoresis followed by azo-dye-coupled staining procedures, zones of esterase activity were classified as A , B, and C. Population variants of these esterase enzymes have proven to be either exceedingly rare or nonexistent and consequently have no forensic application. www.astm.org /JOURNALS/FORENSIC/PAGES/6068.htm   (159 words)

Complement C1 Esterase Inhibitor, Functional This method provides C1 esterase function activity as a percentage of mean normal activity. Esterase inhibitor is decreased in hereditary angioneurotic edema. Functional level will be decreased when concentration of inhibitor is decreased or inhibitor is nonfunctional. www.labcorp.com /datasets/labcorp/html/chapter/mono/sc032300.htm   (223 words)

Human Salivary Tea Catechin Levels and Catechin Esterase Activities: Implication in Human Cancer Prevention Studies -- ...   (Site not responding. Last check: 2007-10-29) The data points are the means of the duplicate determinations of samples obtained from the experiment described in the Fig. To determine whether the esterase is a human enzyme or an enzyme of this esterase activity is still not clear. cebp.aacrjournals.org /cgi/content/full/8/1/83   (4544 words)

Adjuvant treatment of severe acute pancreatitis with C1 esterase inhibitor concentrate after haematopoietic stem cell ... Adjuvant treatment of severe acute pancreatitis with C1 esterase inhibitor concentrate after haematopoietic stem cell transplantation -- Schneider et al. C1 esterase inhibitor is the most important inhibitor of the classical Combined treatment with C1 esterase inhibitor and antithrombin III improves survival in severe acute experimental pancreatitis. gut.bmjjournals.com /cgi/content/full/45/5/733   (2528 words)

Microbial esterase assay - BioForum I have tried fluorescein diacetate but have had a lot of solubility problems, if anyone knows of a method with this compound it would be appreciated. A simple old fashioned method is preparing plates containing 3ml of an emulsion of 50% tributyrin and 5% gum arabic per 100 ml of medium. You will get turbid plates, grwoth of esterase containing bacteria will form a clear halo around the colony. www.protocol-online.org /forums/index.php?showtopic=1646   (184 words)

Molecular Cloning of the Human Esterase D Gene, a Genetic Marker of Retinoblastoma -- Lee and Lee 83 (17): 6337 -- ... The human esterase D gene, on the other hand, has been localized cytogenetically to the same sub-band of chromosome 13q14:11 as the RB gene. In this communication, we describe the isolation of the esterase D cDNA clone. The availability of this clone should allow for the cloning of the RB gene by chromosome walking; the diagnosis of genetic defects such as retinoblastomas and Wilson disease, whose genes are closely linked to the esterase D gene; and the exploration of the large family of human esterase genes. www.pnas.org /cgi/lookup?vol=83&fp=6337&view=abstract   (485 words)

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