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Topic: Exo III


In the News (Sun 26 May 13)

  
  Exo III - Wikipedia, the free encyclopedia
Exo III is a Class P world covered in ice.
Exobiologist, Dr. Roger Korby remained on Exo III for five years studying the ancient ruins.
He discovered a machine that made realistic androids, and with the help of Ruk, one of the remaining original androids, Korby plotted to replace key personnel in Starfleet and the U.F.P. with android clones under his control.
www.wikipedia.org /wiki/Exo_III   (178 words)

  
 Exo III - Memory Alpha
Exo III is the third planet of the Exo system.
In 2266, the USS Enterprise, under command of Captain Kirk, travelled to Exo III to learn the fate of the Korby expedition.
Korby revealed that the civilization of Exo III had fled the surface as the planet began to cool, eventually exchanging their open, free society for an increasingly mechanistic one.
www.memory-alpha.org /en/wiki/Exo_III   (384 words)

  
 [No title]
Exo III footprints (i.e.the boundary for the protein nearest to the labeled end) are seen as bands intermediate between the undigested probe and the complete digestion products.
Unlike in the exo III titration, where the DNA steadily progresses from undigested to completely digested, with decreasing binding protein the DNA should pass directly from undigested to completely digested, since for each molecule of DNA, either the ends are covered by nonspecific binding or not.
If the binding protein titration looks like the exo III titration, then it is probably due to inhibition of exo III activity by the protein sample.
wheat.pw.usda.gov /~lazo/methods/iubio/exoiiifoot.html   (713 words)

  
 [No title]
These vectors are specifically designed for Exo III deletions because the restriction sites in the polylinker are arranged so that the 3'-overhang enzyme sites are located on the edges of the polylinker and the 5'-overhang restriction sites are clustered in the interior region.
The Exo III deletions may not show perfect progression in the comparison of size to the length of time exposed to the Exo III enzyme.
This is not unusual because Exo III is a processive enzyme which will take a single molecule of DNA through a complete digestion before it is released and free to bind another DNA molecule.
wheat.pw.usda.gov /~lazo/methods/iubio/exoiii.html   (956 words)

  
 [No title]
A crystal of Endo III soaked in thymine glycol, an end-product inhibitor of the enzyme, showed a small change in the difference electron density close to a hairpin-loop formed by residues 113-119 in the amino-terminal domain.
Glu 112 in endonuclease III was originally suggested as the functional homologue of Glu 23 in T4 endo V, as it lies close to the putative thymine glycol binding site, and is surrounded by many basic residues, mirroring the environment of Glu 23 in the T4 endo V structure.
The Exo III fold is very similar to DNase I and to RNase-H, which appear to constitute a structural superfamily of double-strand specific nucleases (Figure 6).
www.biochem.ucl.ac.uk /bsm/xtal/teach/repair/tibs3.html   (3997 words)

  
 Encyclopedia: Exo III   (Site not responding. Last check: 2007-10-15)
In the fictional universe of Star Trek, Exo III is a planet vishited in the Star Trek: The Original Series episode, What are Little Girls Made Of?
In the fictional Star Trek universe the Planet Classification System is a system developed by the Federation to categorize planets by many factors, such as atmospheric composition, age, surface temperature, size, and presence of life.
Astrobiology (in Greek astron = star, bios = life and logos = word/science), also known as exobiology (Greek: exo = out) or xenobiology (Greek: xenos = foreign) is the term for a speculative field within biology which considers the possible variety of extraterrestrial life.
www.nationmaster.com /encyclopedia/Exo-III   (438 words)

  
 EXONUCLEASE III DELETIONS FOR SEQUENCING
Exo III tends to delete more quickly early in the reaction, therefore, it is a good idea to take 30 second aliquots for the first two minutes.
For S1 nuclease, aliquot the Exo III samples into EDTA to stop the reaction and immediately place it on dry ice.
(An example of volumes is 10 ul of Exo III stopped with 1.5 ul of 10X N. crassa buffer, 11.5 ul of N. crassa, 11.5 ul of Klenow reaction mix.) Incubate 30 minutes at room temperature.
wheat.pw.usda.gov /~lazo/methods/lazo/exonuclease.html   (1166 words)

  
 Old Ones (Exo III) - Memory Alpha
The Old Ones is the name given to the race that lived on Exo III by one of their creations, the android Ruk.
When the surface of Exo III began to cool, its people moved underground, sacrificing their open environment for the possibility of survival within the dark caves.
In 2266, Captain Kirk visited Exo III attempting to contact Korby.
memory-alpha.org /en/wiki/Old_Ones_%28Exo_III%29   (323 words)

  
 Exonuclease III
Exonuclease III is a 3'-5' exonuclease specific for double-stranded DNA.
Exonuclease III is used in conjunction with S1 nuclease for unidirectional deletion of sequences from the termini of DNA fragments.
Purified from E. coli strain harbouring the plasmid with Exo III gene.
www.bioron.net /exonuclease_iii.htm   (241 words)

  
 EPICENTRE®—Reagents and Kits for Genomics, Proteomics, and RNA Research
Exonuclease I (Exo I) is a single-strand-specific exonuclease that digests DNA in a 3´-5´ direction (Figure 2).
Exo I conveniently removes residual primers and single-stranded DNA from amplification reactions, creates blunt ends from 3´-overhangs, and removes single-stranded DNA from nucleic acid mixtures.
The primary use for Exo III is in conjunction with Mung Bean Nuclease or another single-strand-specific nuclease for generation of unidirectional sets of nested deletions for structure-function studies or dideoxy sequencing.
www.epicentre.com /f2_1/f2_1n.asp   (1286 words)

  
 Exonuclease III (E. coli), Nucleases, New England Biolabs
The preferred substrates are blunt or recessed 3´-termini, although the enzyme also acts at nicks in duplex DNA to produce single-strand gaps.
Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications.
Exonuclease III has also been reported to have RNase H, 3´-phosphatase and AP-endonuclease activities (1).
www.neb.com /nebecomm/products/productM0206.asp   (579 words)

  
 Star Trek - The Original Series, Episode 10: What Are Little Girls Made Of? - Video
The Enterprise orbits around Planet Exo III to try and find Dr. Roger Korby "The Pasteur of Archeological Medicine." Korby was also the fiance' of Enterprise Nurse Christine Chapel.
Kirk is captured by Ruk and, to Christine's horror, is locked down naked on a giant turn table and is about to be duplicated into an android himself, but not before the resourceful captain plants an ethnic slur during the duplicating process into his double's mind to warn Spock.
A truly great episode, with the erie and dark caverns of Exo III to scare the daylights out of you, not to mention Ted Casiday in the scary make-up and deep sinister voice as Ruk.
video.lowpriced.org /6300213145.html   (1028 words)

  
 Dictionary of Meaning www.mauspfeil.net
Exo III is a Star Trek planet classifications Class P world covered in ice.
He discovered a machine that made realistic androids, and with the help of Ruk, one of the remaining original androids, Korby plotted to replace key personnel in Starfleet and the United Federation of Planets U.F.P. with android clones under his control.
Korby's plan was later foiled by the crew of the starship ''starship Enterprise U.S.S. Enterrpise'' when they arrived on Exo III.
www.mauspfeil.net /Exo_III.html   (202 words)

  
 Centromeric polymerase III transcription units in Chironomus pallidivittatus -- Rovira and Edstrom 24 (9): 1662 -- ...
First bases in cloned fragments remaining after Exo III deletions from the 5'-end of pCp254 are indicated in subsequent line by numbers used in lanes for electrophoretic separations of Figure 7B.
Furthermore, DNA motifs for pol III transcription are not localized to the 5'-part of the element, which is probably necessary for transposition.
In this respect transcription control may differ from that of some insect pol III transcribed genes which may be influenced by relatively large 3' flanking regions (23, 24).
nar.oupjournals.org /cgi/content/full/24/9/1662   (5000 words)

  
 Global genome removal of thymine glycol in Escherichia coli requires endonuclease III but the persistence of processed ...
on the nth gene that encodes the endonuclease III glycosylase.
the nth gene that encodes the endo III glycosylase.
Exo III then cleaves the phosphodiester bond 5' to the AP site producing a normal 3' terminus and a gap of 1 nt.
nar.oupjournals.org /cgi/content/full/30/21/4583   (5572 words)

  
 Tramadol, buy tramadol, Ultram, antibiotics, Weight Loss drugs, weight loss herbs, weight loss herbal formula, hair ...   (Site not responding. Last check: 2007-10-15)
Two herpes simplex virus mutants containing mutated residues within the conserved Exo III motif of the polymerase gene were previously shown to be defective in 3'-5' exonuclease activity and exhibited extremely high mutation frequencies.
Marker transfer experiments and sequencing analyses demonstrated that these altered phenotypes were the result of mutations within the Exo III motif.
The data indicate that, aside from leading to exonuclease deficiency, mutations in the Exo III motif may also affect interaction of nucleoside triphosphates with the catalytic sites of polymerase activity.
www.darwinpharmacy.com /ref-acyclovir/acyclovir-research-abs3.233.html   (563 words)

  
 The 3'right-arrow5' exonuclease of DNA polymerase delta can substitute for the 5' flap endonuclease Rad27/Fen1 in ...
Exo I, Exo II, and Exo III motifs that inactivate its exonuclease,
of the rad27 or pol3-Exo mutation.) Mutations in the Exo I and
Exo I, Exo II, and Exo III motifs was examined in this study.
www.pnas.org /cgi/content/full/98/9/5122   (4284 words)

  
 Sector 001: Personnel B   (Site not responding. Last check: 2007-10-15)
Bennet was killed in a shuttlecraft crash in 2372 during a geological survey mission to one of the moons of Drayan II.
In 2370 she and her sister illegally mined a magnesite ore deposit on Kalla III that belonged to the Pakleds.
Citizen of the society on planet Beta III who was under the control of the machine entity Landru.
www.powernet.net /%7Ejcrafton/person-b.html   (9229 words)

  
 Interspecific effects of pheromones on the attraction of the bark beetles, Dendroctonus brevicomis and Ips paraconfusus ...   (Site not responding. Last check: 2007-10-15)
The objectives of this study were to investigate the responses of these beetles to semiochemicals, both intra- and interspecific, under laboratory conditions.
I, III, E, and F were all racemic, the enantiomeric composition of II was not known, and verbenone was [a]
Further experiments with various mixtures of I, II, and III are needed to elucidate which compounds are involved.
www.wcrl.ars.usda.gov /cec/papers/jce81.htm   (3086 words)

  
 Android - Memory Alpha
The Old Ones of Exo III discovered how to build sophisticated androids thousands of years ago.
Ruk and all other known Exo III type androids were destroyed in 2266.
In 2372, USS Voyager found a deactivated android which, upon repair by B'Elanna Torres, identified itself as Automated Personnel Unit 3947.
www.memory-alpha.org /en/index.php/Android   (413 words)

  
 Sheryl's Star Trek Site: Star Trek Women: Classic Star Trek:Chapel
Christine Chapel began her medical career aboard the U.S.S. Enterprise, sacrificing a biology career with several university degrees in research medicine to search for her lost fiance, Dr. Roger Korby, who had been missing for five years, ever since his last communication on Exo III.
When Korby was found on Exo III in 2266 and subsequently discovered to be an android, Chapel elects to remain with the starship, where she became good friends with Uhura and a good friend to Dr. McCoy.
Emotionally, her early shipboard life, as admitted when infected with the Psi 2000 virus, was marked by an acknowledged unrequited love for Spock - a ongoing issue which never interfered with her professional duties.
www.sherylfranklin.com /trekwomen_chapel.html   (319 words)

  
 "Classic-Guide- Season 1 Besprechung   (Site not responding. Last check: 2007-10-15)
Die Enterprise nähert sich dem Planeten Exo III, auf dem sich zuletzt der seit langem vermißte Dr. Roger Korby, der Verlobte von Christine Chapel, aufgehalten hat.
Als Spock ihn anspricht, schnauzt Kirk ihn an und nennt ihn ein "Halbblut" und kehrt wieder auf Exo III zurück.
Exo III ist der blau-schwarze Planet wie Rigel XII.
www.sftv.ch /hoehl/tos/tos-09.htm   (1141 words)

  
 [No title]
Exo III falls behind us and, with it, the dreams of a crewmember who had traveled parsecs on a quest of personal import.
When the security guards and I finally located Captain Kirk and Miss Chapel in the depths of the Exo caverns, it was obvious that we had burst in on the closing moments of a dramatic scene.
We stood in reverence and memory of Rayburn and Matthews, the two guards killed on the surface, and then for Dr. Roger Korby, Dr. Amos Brown, and the rest of his team that had died on Exo III.
www.geocities.com /falconprss/betrothd.html   (1382 words)

  
 [základna Antares]
Civilizace na Exo III (mimochodem stejně jako Lovecraftovi Starší posedlá nepravoúhlou geometrií, především pětiúhelníky) dopadla velmi podobně.
Nikdy se nedozvíme, co se tenkrát v jeskyních na Exo III stalo, ale je zřejmé, že celá výprava zahynula.
Pozorný čtenář teď namítne, že nelze srovnávat utlačované androidy z Exo III s podlými Shoggothy, kteří dokázali jen ničit a zabíjet.
www.sf.cz /service.php?akce=tisk2&cisloclanku=2003110801   (840 words)

  
 Exonuclease III and the Catalase Hydroperoxidase II in Escherichia coli are Both Regulated by the katF Gene Product -- ...
The levels of both exonuclease III (exo III, product of xthA) and hydroperoxidase II (HP-II, product of katE) activity in Escherichia coli were influenced by a functional katF gene.
Exo III, encoded by the xthA locus, recognizes and removes nucleoside 5'-monophosphates near apurinic and apyrimidinic sites in damaged DNA.
We propose that the katF gene is a trans-acting positive regulator of exo III and HP-II enzymes, both of which are involved in cellular recovery from oxidative damage.
www.pnas.org /cgi/content/abstract/86/9/3271   (819 words)

  
 Information for the Role of Christine Chapel   (Site not responding. Last check: 2007-10-15)
Christine Chapel havde en lovende karriere i Stjerneflåden indenfor bioresearch, men hendes forlovede, dr. Roger Korby, forsvandt i 2261 på Exo III og Chapel besluttede at droppe sin karriere for at lede efter ham.
Chapels håb er, at hun ved at deltage i den 5 år lange mission vil få mulighed for at blive genforenet med sin forlovede.
Hun får muligheden, da Enterprise i 2266 ankommer til Exo III.
sttos.epguides.info /?Lookup=Roles:1535   (803 words)

  
 Nucleases: Exonuclease III
Rogers, S.G., Weiss, B., Exonuclease III of Escherichia coli K-12, an AP endonuclease, Methods Enzymol., 65, 201-211, 1980.
Guo, Li-He., Wu, R., New rapid methods for DNA sequencing based on exonuclease III digestion followed by repair synthesis, Nucleic Acids Res., 10, 2065-2084, 1982.
Tomb, J.F., Barcak, G.J., Regulating the 3'-5' activity of exonuclease III by varying the sodium chloride concentration, BioTechniques, 7, 932-933, 1989.
www.fermentas.com /catalog/modifyingenzymes/exonucleaseiii.htm   (372 words)

  
 Selective blockage of the 3'-->5' exonuclease activity of WRN protein by certain oxidative modifications and bulky ...   (Site not responding. Last check: 2007-10-15)
In this study, we have further characterized WRNp exonuclease activity by examining its ability to degrade double-stranded DNA substrates containing abnormal and damaged nucleo-tides.
The ability of damaged nucleo-tides to inhibit exonucleolytic digestion differs significantly between WRNp, exonuclease III and Klenow, indicating that each exonuclease has a distinct mechanism of action.
In addition, normal and modified DNA substrates are degraded similarly by full-length WRNp and an N-terminal fragment of WRNp, indicating that the specificity for this activity lies mostly within this region.
www.arclab.org /medlineupdates/abstract_10908333.html   (190 words)

  
 The C-Terminal Domain of DnaQ Contains the Polymerase Binding Site -- Taft-Benz and Schaaper 181 (9): 2963 -- The ...
Mutants in the Exo I motif of Escherichia coli dnaQ: defective proofreading and inviability due to error catastrophe.
Isolation and characterization of mutants with deletions in dnaQ, the gene for the editing subunit of DNA polymerase III of Salmonella typhimurium.
Role of the core DNA polymerase III subunits at the replication fork.
jb.asm.org /cgi/content/full/181/9/2963   (1686 words)

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