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Topic: Expression vector


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In the News (Sat 25 May 19)

  
  Expression vector - Wikipedia, the free encyclopedia
Once the expression vector is inside the cell, the protein that is encoded by the gene is produced by the cellular transcription and translation machinery.
Expression vectors are used for molecular biology techniques such as site-directed mutagenesis.
Expression vectors are often specifically designed to contain regulatory sequences that act as enhancer and promoter regions, and lead to efficient transcription of the gene that is carried on the expression vector.
en.wikipedia.org /wiki/Expression_vector   (205 words)

  
 ATCC: Vector Diagrams
Expression vector for rapid purification of fusion proteins and release of proteins with fewer extraneous N-terminal amino acids.
This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation.
Expression vector encoding the beta-galactosidase alpha peptide (lacZ'), permitting blue-white visual detection of re combinants.
www.atcc.org /common/products/vectors.cfm   (5301 words)

  
 Expression Templates (Todd Veldhuizen)
In C, expressions are usually passed using a pointer to a callback function containing the expression.
To evaluate the efficiency of the expression templates approach to evaluating vector expressions, benchmark results were obtained comparing its performance to hand-crafted C code, and that of a conventional vector class.
For longer vectors (eg., length 100) the benchmark results were 95-99.5% the speed of hand coded C. Figure 6 compares the expression template technique to a conventional vector class which evaluates expressions using temporaries.
osl.iu.edu /~tveldhui/papers/Expression-Templates/exprtmpl.html   (2492 words)

  
 [No title]   (Site not responding. Last check: 2007-10-08)
Expression vectors of utility in the present invention are often in the form of"plasmids", which refer to circula double stranded DNA loops which, in their vector form, are not bound to the chromosome.
Expression of the marker gene in response to induction or selection indicates expression of the DNA sequence coding for the heterologous protein.
The DNA sequences of expression vectors, plasmids or DNA molecules of the present invention may be determined by various methods known in the art.
www.wipo.int /cgi-pct/guest/getbykey5?KEY=99/31220.990624&ELEMENT_SET=DECL   (4739 words)

  
 Ambion TechNotes 9(4): pSilencer siRNA Expression Vector
Expression of siRNA in Vivo Elicits Gene Silencing
This vector was developed by Sui and colleagues at Harvard Medical School and has been successfully used to knock down expression of cdk-2 and lamin A/C in HeLa, H1299, U-2 OS and C-33A (cdk-2 only) cells (4).
Plasmid Vector Expression of siRNA Cause Gene Silencing.
www.ambion.com /techlib/tn/94/941.html   (886 words)

  
 A novel expression vector composed of a regulatory element of the human leukosialin-encoding gene in different types of ...
A novel expression vector composed of a regulatory element of the human leukosialin-encoding gene in different types of mammalian cells.
The CAT activity of the pKX vector was compared to the other common expression vectors, namely pMSG (driven by the mouse mammary tumor virus LTR), pcDL-SR alpha (SV40 promoter/enhancer and HTLV-I LTR), pcDNAI (cytomegalovirus promoter/enhancer) and pCAT-Control (SV40 promoter/enhancer).
Potential uses of this vector may be found in studies of transient gene expression in hematopoietic cells and for gene therapy, particularly the ones involving T-cells.
www.aegis.com /aidsline/1995/nov/M95B0211.html   (477 words)

  
 Biocompare - Product Literature Center
Vectors with convenient options, such as the capacity for high-fidelity PCR cloning and epitope tagging for characterization studies, have become integral to sophisticated research inquiries.
The pCMV-Tag2 vector contains sequences for N-terminal tagging with the FLAG epitope, and the pCMV-Tag3 vector is used for N-terminal tagging with the c-myc epitope.
Thus, the pCMV-Tag1 through 5 vectors include constitutive expression of the cloned DNA in a wide variety of mammalian cell lines and the capability of selecting stable transformants by kanamycin resistance in bacteria and G418 resistance in mammalian cells.
www.biocompare.com /techart.asp?id=206   (1825 words)

  
 [No title]   (Site not responding. Last check: 2007-10-08)
This vector has the potential to translate approaches developed at the cellular level to pre-clinical models to clinical applications and has been tested in N2a (a murine neuroglial cell line) and 293T (human embryonic kidney cell line) by transient transfection and is currently under validation in other cell lines.
Expression vectors suitable for practice of the present invention are well known in the art and depend, in part, on the cell type in which expression will be monitored.
Where expression of a particular gene is of interest, expression control sequences for that gene of interest may be incorporated into the expression vector, as will be appreciated by the skilled artisan.
www.wipo.int /cgi-pct/guest/getbykey5?KEY=04/81189.050217&ELEMENT_SET=DECL   (8715 words)

  
 Imgenex : Expression Systems
The RetroMax™ Retroviral Expression vector system is based on the pCL vector system and has been designed to maximize recombinant—retrovirus titers in a simple, efficient, and flexible experimental system1.
All members of the RetroMax expression vector family (pCLXSN, pCLNCX, pCLNRX, and pCLNDX) have an extended packaging signal and are derived from safety- modified retrovirus vectors in which the gag open reading frame has been stopped by a point mutation, thereby minimizing the opportunity for replication competent retrovirus production by recombination with packaging genome.
Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells to permit infection of more than 50% of dividing target cells in culture.
www.imgenex.com /expression_systems.php   (490 words)

  
 Majnik/Weinman   (Site not responding. Last check: 2007-10-08)
Three cassettes for the expression of a cloned coding sequence behind different promoters have been modified; combinations of these cassettes can be excised with Not I, and sequentially cloned into the transformation vector in a procedure that removes the first cloning site.
If an expression cassette needs to be excised and introduced into another vector (such as a binary vector) for transfer to plant cells via Agrobacterium, the procedure can become complicated with the possibility of restricting the gene during the cloning process.
These problems are compounded if a binary vector carrying t wo expression cassettes is constructed, with the added possibility of restricting within the first introduced cassette during the second cloning.
www.uga.edu /ispmb/Pmbr15-2/MajnikWeinman.html   (1446 words)

  
 Expression vectors
Expression of Ptac is repressed by the LacI protein.
If this leaky expression causes problems, it may be necessary to clone the gene into an alternative expression vector that is more tightly repressed.
Using the temperature sensitive cI857 repressor allows control of gene expression by changing the growth temperature -- at 30 C the cI857 repressor is functional and it turns off expression of the gene, but at 42 C the repressor is inactivated so expression of the gene ensues.
www.sci.sdsu.edu /~smaloy/MicrobialGenetics/topics/in-vitro-genetics/expression-vectors.html   (723 words)

  
 Matrix Expression Templates
Thus, in addition to providing the ready-to-use matrix and vector classes, MET serves as a code basis for extending your classes to be more notationally convenient but suppress the superfluous overhead.
The expression template is implemented with container classes for holding the data of vector or matrix elements, along with a set of global template functions which provides the closure object construction mechanism.
User-defined matrix and vector classes would be derived from these base classes to be eligible to be an terminal in an expression-template parse tree.
met.sourceforge.net   (924 words)

  
 Kurt von Behrmann reviews Creature House Expression 3
Using their own graphic engine, Expression was able to do what few applications dared, and with a great degree of power.
Expression, as well as Painter, were both extensions of the idea that computer-based tools could and should be accessible to artists.
While the design philosophy behind Expression was to create a relatively easy-to-use application with power to spare, I found that Expression, while not the most difficult program to use, still has a steep learning curve.
www.unleash.com /kurt/expression3/index.asp   (1904 words)

  
 The pET Expression System   (Site not responding. Last check: 2007-10-08)
Expression systems are designed to produce many copies of a desired protein within a host cell.
This vector contains all of the genetic coding necessary to produce the protein, including a promoter appropriate to the host cell, a sequence which terminates transcription, and a sequence which codes for ribosome binding (Purves et al., 2001).
One expression system was developed in 1986 by W. Studier and B. Moffatt, who created an RNA polymerase expression system which was highly selective for bacteriophage T7 RNA polymerase.
bio.davidson.edu /Courses/Molbio/MolStudents/spring2003/Causey/pET.html   (837 words)

  
 Tony Schreiner's WebLog : Expression 3: Vector drawing application available for download
I looked at the expression page a few days ago and it was 50 megs and no point trying.
With PD one was too scared to move the mouse as it might force a long and slow redraw.
But windows has always been anti vector, office which was vector gave no way of saving your files.
blogs.msdn.com /tonyschr/archive/2004/06/07/150389.aspx   (572 words)

  
 BD Biosciences Canada : Pharmingen : BD BaculoGold™ Baculovirus Expression Vector System
The Baculovirus Expression Vector System is based on the introduction of a foreign gene into a nonessential region of the viral genome via homologous recombination with a transfer vector containing the cloned gene; an event that occurs in the cotransfected insect cells.
Co-transfection of the BD BaculoGold DNA and a complementing baculovirus transfer vector restores viability by homologous recombination and rescues the virus along with the desired recombinant gene.
Vectors are also available in three translational reading frames for expression of proteins which are singularly- or multiply-tagged with 6xHis, GST or a signal peptide for protein secretion.
www.bdbiosciences.ca /canada/pharmingen/product_pages/baculovirus   (1120 words)

  
 SIUC COS Microbiology 421 - chapter 7
Expression is induced during late stages of infection of insect cells
Homologous recombination between the transfer vector and the baculovirus expression vector transfers the cloned gene to the expression vector
Expression of both genes may not be balanced, ratio of protein chains may not be be correct for formation of functional multemeric protein
www.science.siu.edu /microbiology/micr421/chapter7.html   (716 words)

  
 Recombinant Protein Expression Laboratory   (Site not responding. Last check: 2007-10-08)
Initially, the Recombinant Protein Laboratory will specialize in the use of the baculovirus expression vector system (BEVS), a eukaryotic expression system that has been successfully used to express a large variety of proteins.
Depending upon the level of expression obtained for a particular protein, quantities ranging from hundreds of micrograms to tens of milligrams can be readily produced using resources at the facility.
The facility is capable of providing bacterial and yeast expression services as well.
www.cbs.umn.edu /bpti/RPEL.html   (187 words)

  
 [No title]
In addition, the receptors are bound to the recognition element in the absence of inducer, further reducing background expression.
The expression vector, pEGSH, is designed for easy detection of expressed protein by either RNase protection assay using T3 antisense probes or by western analysis using the FLAG® epitope.
The two retroviral vectors are pFB-ERV, an MMLV-based replication-defective retroviral vector that delivers the ecdysone receptor proteins RXR and VgEcR, and the pCFB-EGSH retroviral vector containing the inducible cassette.
www.stratagene.com /products/displayProduct.aspx?pid=247   (1174 words)

  
 Fox Chase Cancer Center: Fox Chase Cancer Center Office of Business Development   (Site not responding. Last check: 2007-10-08)
This vector is ideal for use in gene therapy, especially in cases of hematopoietic malignancies.
It is expressed in thymus, spleen, and testis, and in the mouse, is thought to contribute to induction and progression of hematopoietic neoplasms such as T cell lymphomas.
In addition to the implications of gfi-1?s role in the biology of HCMV, HCMV is a commonly utilized expression vector.
www.fccc.edu /techtransfer/techbriefs/mutHCMV.html   (443 words)

  
 Porcine adenovirus-3 as a helper-dependent expression vector -- Reddy et al. 80 (11): 2909 -- Journal of General ...
Porcine adenovirus-3 as a helper-dependent expression vector -- Reddy et al.
Khatri, A., Xu, Z. and Both, G. Gene expression by atypical recombinant ovine adenovirus vectors during abortive infection of human and animal cells in vitro.
Van den Elsen, P., Houweling, A. and Van der Eb, A. Expression of region E1b of human adenoviruses in the absence of region E1a is not sufficient for complete transformation.
vir.sgmjournals.org /cgi/content/full/80/11/2909   (4402 words)

  
 Construction of a Sorbitol-Based Vector for Expression of Heterologous Proteins in Saccharomyces cerevisiae -- ...
Construction of a Sorbitol-Based Vector for Expression of Heterologous Proteins in Saccharomyces cerevisiae -- McGonigal et al.
Construction of a Sorbitol-Based Vector for Expression of Heterologous Proteins in Saccharomyces cerevisiae
To demonstrate the utility of the new vector, we have overexpressed the coding sequence of a yeast translation elongation
aem.asm.org /cgi/content/full/64/2/793   (1031 words)

  
 K. lactis Protein Expression Kit, Yeast, New England Biolabs
Second, yeast expression is driven by a variant of the strong LAC4 promoter that has been modified to lack background expression in E.
In the nucleus, an integrated expression vector encoding a fusion between the α-MF domain (blue) and a desired protein (fl) is expressed.
The vector can be linearized by digestion with Sac II or BstX I to create a linear DNA fragment capable of inserting into the native LAC4 promoter region of the K.
www.neb.com /nebecomm/products/productE1000.asp   (920 words)

  
 Cloning and Molecular Analysis of Genes   (Site not responding. Last check: 2007-10-08)
The two molecules that are required for cloning are the DNA to be cloned and a cloning vector.
Cloning vector - a DNA molecule that carries foreign DNA into a host cell, replicates inside a bacterial (or yeast) cell and produces many copies of itself and the foreign DNA
prepare the vector and DNA to be cloned by digestion with restriction enzymes to generate complementary ends
www.ndsu.nodak.edu /instruct/mcclean/plsc431/cloning/clone3.htm   (296 words)

  
 Expression vector/host for plant genomic sequence - BioForum
Is there any point in using E.coli (prokaryote host expression system) or should I just use a eukaryote expression system (yeast).
I am not too familiar with vectors to use in yeast, but I think you would have to use a Yeast artificial chromosome to clone you gene into so there is an origin of replication that the yeast cells can recognize.
An alternative is to use a prokaryote system with a vector (I think Clontech is a good place to go with to find a good prokaryote expression vector) In this case, you would have to get rid of the introns of course.
www.protocol-online.org /forums/index.php?showtopic=985   (413 words)

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