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Topic: FITC

In the News (Tue 2 Dec 08)

Damian Green

Sumner Redstone

Crimson Tide

Tommy Tuberville

Bill Clinton

Wayne Rooney

Vilasrao Deshmukh

Wake Forest

Grand Slam

Oklahoma State

	FITC conjugation of Antibodies
		FITC is a small organic molecule, and is typically conjugated to proteins via primary amines (i.e., lysines).
		The extent of FITC conjugation to the antibody may depend on the concentration of antibody in solution; for consistent conjugations, use a consistent concentration.
		FITC is covalently coupled to primary amines (lysines) of the immunoglobulin.
	www.drmr.com /abcon/FITC.html (769 words)

	FITC conjugation of Annexin V
		This protocol is nearly identical to that of conjugation of FITC to immunoglobulins.
		The extent of FITC conjugation to the Annexin V may depend on the concentration of Annexin V in solution; for consistent conjugations, use a consistent concentration.
		FITC is covalently coupled to primary amines (lysines) of the Annexin V. Dissolve 10 mgs (the entire contents of 1 vial; no need to weigh) of FITC in anhydrous DMSO immediately before use.
	www.drmr.com /abcon/AxVFITC.html (837 words)

	FITC Awards 2006 (Site not responding. Last check: 2007-11-02)
		FITC assumes that all entries are original and are the works and property of the entrants, with all rights granted therein.
		The FITC Award will only be given to the owner of a site or their authorized representative, regardless of the entrant.
		FITC is not liable for any copyright infringement caused by the entrant or a submission.
	awards.fitc.ca /2006/rules (592 words)

	Gamasutra Calendar
		Now entering our fourth year, FITC is the only Canadian Festival of its kind; combining cutting edge technical speakers with inspirational creative speakers from around the world.
		Each year, FITC has broadened its programming range to grow with its widening audience and appeal to a larger audience.
		FITC has three goals .to inspire, to educate, and to challenge, all within the Design and Technology space.
	www.gamasutra.com /php-bin/calendar.php?event_id=585 (140 words)

	FITC (Site not responding. Last check: 2007-11-02)
		FITC is giving away 10 tickets to those in need.
		FITC presents Flash 8 workshop w/ Grant Skinner
		FITC 2006 will be held on April 21 - 23 (Fri-Sun) of 2006 at the Westin Harbour Castle.
	www.fitc.ca (355 words)

	Spectroscopy of Fluorescein (FITC) Dyed Colloidal Silica Spheres
		We have measured the absorption spectrum, the emission spectrum, the emission lifetime, and the photostability of fluorescein isothiocyanate (FITC) incorporated inside colloidal silica spheres as a function of the dye concentration in the spheres, while minimizing scattering effects.
		Six batches of stable, monodisperse particles were synthesized with FITC up to high densities of 0.03 M. At dye concentrations above 0.001 M, we observe a large red shift of 10 nm in the absorption and the emission spectra, as well as a strong reduction of the lifetime.
		It is suggested that the dye molecules tend to form clusters during the synthesis of the colloidal spheres.
	pubs.acs.org /cgi-bin/abstract.cgi/jpcbfk/1999/103/i09/abs/jp983241q.html (264 words)

	Nikon MicroscopyU: Fluorescence Microscopy - Dual Band Excitation Filter Combinations - FITC-TRITC
		This filter set is designed for optimal detection of the traditional fluorescein isothiocyanate (FITC) and tetramethylrhodamine isothiocyanate (TRITC) probes in combination, as well as other fluorophores with similar absorption and emission spectra.
		The dual excitation interference filter incorporates an excitation filter with narrow passband windows in the blue (475 to 490 nanometers) and the green (540 to 565 nanometers) spectral regions.
		The shorter wavelength signal channel of the filter set selects narrowly defined spectral regions for blue excitation and green emission detection, while the longer wavelength component corresponds to green excitation and orange-red emission detection.
	www.microscopyu.com /articles/fluorescence/filtercubes/dual/fitctritc/fitctritcindex.html (1120 words)

	IC - Faculty Instructional Technology Center (FITC)
		Please email fitc@cats or call 459-5506 to make an appointment with one of our staff to arrange for access to the room and to discuss the scope of your equipment use.
		Workstations in the FITC must be reserved in advance by using the online Workstation Schedule.
		FITC staff are available on a limited basis to teach workshops in web publishing technology for students and faculty.
	ic.ucsc.edu /labs/labdescriptions/fitc (267 words)

	anti-Survivin, FITC Conjugated CytoLight(TM) Kit > Assay Designs (Site not responding. Last check: 2007-11-02)
		This monoclonal antibody is produced by immunizing mice with a recombinant protein to develop a clone cell line.
		The resulting cell supernatants are purified using a Protein G column and were conjugated to FITC fluor under optimum conditions.
		The antibody should be stored undiluted at 2- 8°C and protected from prolonged exposure to light.
	www.assaydesigns.com /products/catalog/detection_kits/product_FITC_Survivin.htm (375 words)

	Olympus Microscopy Resource Center: Fluorescence Microscopy Image Gallery - PtK2 Cell Microtubules and Nucleus
		A vivid contrast between nuclear and cytoplasmic structure is evidenced by staining cell cultures with a combination of propidium diiodide (PI) and FITC.
		The specimen is PtK2 marsupial kidney epithelial cells grown in tissue culture, then fixed and double-stained using FITC and propidium diiodide (PI).
		Photomicrographs were recorded utilizing a UPL 100x apochromatic objective coupled to a WU dichroic filter combination.
	www.olympusmicro.com /galleries/fluorescence/pages/p2k2microtubulescellnucsmall.html (515 words)

	FITC Toronto 2006 - The Design & Technology Festival (Site not responding. Last check: 2007-11-02)
		Now in its fifth year, FITC 2006 will be our biggest and best festival ever!
		With over a thousand attendee's anticipated from around the Globe, it promises to be an event unlike any other.
		We're bringing some of the most interesting and engaging presenters from around the world to Toronto, all for a 3 day and night festival that will leave you inspired and awed.
	www.fitc.ca /event_detail.cfm?festival_id=5 (119 words)

	Color changing karyotyping (CCK)
		The same three dyes are used in the form of fluorescent nucleotides (for example, AMCA-, FITC and rhodamine-dUTP) and as labeled antibodies, to detect the haptenes (bio-, dig- or DNP-dUTP) used to label the chromosomes.
		Thus, although in the first image, the chromosome labeled with FITC and the one labeled with FITC+DIG appear to have the same color (green), in the second image (after adding the Cy3-labeled anti-DIG antibody), the chromosome labeled with DIG-dUTP changes (shifts) its color from green to red.
		Thus, imaging the FITC channel will permit visualizaton of only the FITC-detected, DNP-dUTP stained chromosomes, which are very bright and require significantly shorter exposure times.
	info.med.yale.edu /genetics/ward/tavi/CCKprinciple.html (1308 words)

	Nikon MicroscopyU: Fluorescence Microscopy - Blue Excitation Filter Combinations - B-2A
		The set is designed as a general purpose filter combination for blue excitation, with a dichromatic mirror optimized for fluorescein isothiocyanate (FITC) coupled with counter stains such as propidium iodide (PI) or tetramethylrhodamine isothiocyanate (TRITC).
		The 500-nanometer dichromatic mirror cut-on wavelength is the lowest of all the Nikon blue excitation filter combinations, as is the longpass barrier filter cut-on wavelength of 515 nanometers.
		Fluorescence emission in the yellow, orange, and red regions is often visible in images collected using the B-2A filter combination (as illustrated in Figure 2).
	www.microscopyu.com /articles/fluorescence/filtercubes/blue/b2a/b2aindex.html (1065 words)

	Combinatorial labeling principle
		For example, a DNA probe labeled with FITC will fluoresce green, another one labeled with AMCA will fluoresce blue, whereas a third one labeled with FITC and AMCA will fluroesce cyan.
		In FISH, however, a DNA probe is usually labeled with no more than three fluorescent dyes at the same time, so the number of usable combinations is smaller.
		(2) In the bottom image, DNA probes for the centromeres of chromosomes 15 (FITC/green), 17 (Cy3/red) and 18 (FITC and Cy3) were simultaneously hybridized onto human cells.
	info.med.yale.edu /genetics/ward/tavi/CCKcomlbl.html (383 words)

	Ajax vs AFLax, Flash Forward and FITC, beta2 news.. (Site not responding. Last check: 2007-11-02)
		Paul Colton (founder of Xamlon, the company I work for) has some really good points about why Flash is generally a way better solution that js/dhtml/xml.
		If you are just too busy to get there, or they won't let you across the US border, not to worry - we will also be at FITC which is like the day after, 9th - 11th.
		Nice to hear you'll be at FITC and representing Xamlon for Flash.
	blog.debreuil.com /archive/2005/03/29/694.aspx (676 words)

	Shawn Pucknell - FITC - Interview
		Each year FITC seems to make great strides from the previous year.
		So, we've tried to move slowly yet surely into this new space, and hopefully we'll do a good job of it in the coming years.
		The Westin has turned out to be a great venue for FITC.
	www.ventilate.ca /issue09/shawn.html (765 words)

	AlphaScreen® FITC (Fluorescein) Detection Kit, 500 assay points from PerkinElmer - USA (Site not responding. Last check: 2007-11-02)
		AlphaScreen fusion tag detection kits are intended for the detection of proteins, peptides or oligonucleotides that are doubly-labeled with the tag and biotin.
		Or contact us to consult with a PerkinElmer application scientist who can support your assay development and help you choose the right assay platform for your specific needs.
		Anti-fluorescein (FITC) Acceptor Beads - 1 tube containing 50 µL anti-FITC Acceptor beads at 5 mg/mL in 25 mM HEPES, 100mM NaCl, pH 7.4.
	las.perkinelmer.com /Catalog/ProductInfoPage.htm?ProductID=6760605C (394 words)

	FITC/Fluorescein (Site not responding. Last check: 2007-11-02)
		For example, the 3 plots below show Background, CD4 FITC, and CD4 Fluorescein when corrected for a FITC standard.
		Then, both CD4 FITC and CD4 Fluorescein stained samples were run using the same instrumental settings:
		Note that the CD4-Fluorescein emits significantly more in the FL2 (PE) channel than does CD4 FITC when the color compensation is set at 16.6% for FITC.
	facs.scripps.edu /cd4fitc.html (172 words)

	Saturation, Competition, and Specificity in Interaction of Heat Shock Proteins (hsp) gp96, hsp90, and hsp70 with CD11b+ ...
		anti-FITC mAb, conjugation of gp96 to FITC was confirmed (Fig.
		B, hsps and SA are complexed to FITC.
		Homogeneous preparations of hsps, gp96, hsp90, hsp70, and SA were complexed covalently to FITC.
	www.jimmunol.org /cgi/content/full/165/5/2582 (3596 words)

	NanoDrop ND-3300 FITC (Site not responding. Last check: 2007-11-02)
		This module is configured to measure the inherent fluorescence of Fluorescein (FITC) at 515nm.
		Fluorescence is measured at 515 nm following excitation (470 nm) by the Blue LED
		A representative spectrum of a 10 nM Fluorescein sample (diluted NIST SRM 1932) measured on a NanoDrop
	www.nanodrop.com /nd-3300-applications-FITC.html (65 words)

	FITC, antibody
		This clone has been derived from hybridization of SP2/0 myeloma cells with spleen cells of Balb/c mice immunised with FITC-BSA conjugate.
		MoAb 2A3 reacts with both free and conjugated FITC.
		Enzyme-labeled second antibody for immunohistochemistry with FITC-conjugated mouse monoclonal antibodies.
	www.hytest.fi /tuotteet96.php (112 words)

	FITC-conjugated siRNA - BioForum
		The second question is what purpose of FITC is applied here?
		The purpose of FITC is to measure your transfection efficiency.
		In my case I used "alexa fluor 488nm" labeled siRNA as a negative control to be able to do a FACS-analysis.
	www.protocol-online.org /forums/index.php?showtopic=5901 (431 words)

	Scientist Solutions - secondary Ab for FITC
		Awarded a "Blue Ribbon" for excellence by Genetic Engineering News - the most widely read bioindustry publication worldwide.
		I have an specific antibody for my cell marker which conjugated with FITC, can I use secondary biotin anti mouse Ig antibody for IHC ?
		We have done it in Immunohistochemistry by APAAP Technic: used a monoclonal conjugated to FITC and used an anti mouse following by Alkaline anti Alkaline complex and at last stained by a dye developed by alkaline.
	www.scientistsolutions.com /index.php?a=topic&t=1386 (424 words)

	PolySciences, Inc. Product: Flow Check® FITC Intensity Set
		The Flow Check FITC Intensity Set is composed of 6 micron particles labeled with four levels of fluorescence intensity - Low-Brite, Med-Brite, High-Brite, and Maxi-Brite.
		These particles are designed for detection in channel FL1 of the flow cytometer.
		The Flow Check FITC Intensity Set is supplied with an undyed particle for comparison.
	www.polysciences.com /shop/product.asp?pf_id=24256&dept_id=4030 (129 words)

	FlashinTO.com :: View topic - FITC 2005 Winners Announced (Site not responding. Last check: 2007-11-02)
		Congratulations to all FITC 2005 Finalists for their impressive display!
		I have to buy a new laptop now b/c after seeing this, I drooled all over my keyboard and shorted out the whole unit.
		Posted: Thu Sep 15, 2005 8:47 pm Post subject: Re: FITC 2005 Winners Announced
	www.flashinto.com /phpBB2/viewtopic.php?t=2059 (371 words)

	::Advisory Services
		is the consulting arm of the Financial Institutions Training Centre (FITC).
		It was established in 1992 to provide first class consulting services to clients in diverse sectors of the local and global economy.
		Kindly click here to register as a member of the library.
	www.fitc-ng.com /fconsult.htm (379 words)

	FITC anti-mouse/rat Foxp3 Staining Set
		17-0251), and subsequently with 1.0μg FITC anti-mouse/rat Foxp3 (FJK-16s) or FITC Rat IgG2a Iso Cntrl (cat.
		11-4321) using the FITC anti-mouse/rat Foxp3 Staining Set (cat.
		The histogram demonstrates FJK-16s staining (solid peak) and isotype control (hollow peak) after gating on CD4+CD25+ cells.
	www.ebioscience.com /ebioscience/specs/antibody_71/71-5775.htm (1165 words)

	PROTEIN A - FITC from Invitrogen - Biocompare Buyer's Guide
		Home » Cell Biology »...(3) » Protein A FITC » PROTEIN A - FITC
		Protein A (from Staphylococcus aureus, Cowan I strain) is conjugated with fluorescein isothiocyanate (FITC) and is purified by molecular sieving to remove the unbound FITC and unconjugated Protein A. Journal Citations
		View all citations for PROTEIN A - FITC from Invitrogen.
	www.biocompare.com /itemdetails.asp?itemid=69249 (177 words)

	Fluorescein (FITC) antibodies from RDI Divison of Fitzgerald Industries Intl
		Fluorescein (FITC) antibodies from RDI Divison of Fitzgerald Industries Intl
		MoAb 19F/2A3 react with both free and conjugated FITC.
		Not for use in or on humans or animals or for diagnostics
	www.researchd.com /miscabs/trk5F3.htm (440 words)

	Bethyl Laboratories - Goat anti-Rabbit IgG Antibody-h&l FITC conjugated antibodies (Site not responding. Last check: 2007-11-02)
		Bethyl Laboratories - Goat anti-Rabbit IgG Antibody-h&l FITC conjugated antibodies
		Goat anti-Rabbit IgG Antibody-heavy & light chain affinity purified
		Goat anti-Rabbit IgG Antibody-h&l cross adsorbed FITC conjugated
	www.bethyl.com /abdetails.asp?antibodyid=222 (85 words)

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