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| | Chromatography Forum |
 | | Up to now, I have been successfully using He as the carrier gas, at 27 cm/s (optimum) and a split ratio of 30:1 at the injection point (250 degrees C injector temperature, 110 degrees C initial column temperature, 100 kPa head pressure, ramped to 220 degrees C over 25 min at constant pressure). |
 | | This suggests to me that: (1) the column is overloaded, or (2) the higher BP FAMEs are having problems getting onto the column, or (3) the higher BP fames are having diffusional problems between the stationary phase and the gas phase in the presence of H2. |
 | | I calculated that at the inlet pressure I use, the H2 flow and velocity (60 cm/s) is about double that for He, and in the optimum range for H2. |
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