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Topic: Feulgen

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	Blue Feulgen Stain
		The combination of acid hydrolysis and subsequent aldehyde staining with a Schiff reagent constitutes the Feulgen procedure (Feulgen was one of two individuals who first developed and described the procedure).
		Schiff regent is commonly used in histology for both the Feulgen procedure and for the Periodic Acid Schiff (PAS) procedure.
		It is also possible to combine ImagePath’s blue Feulgen staining of the cell nucleus with a standard PAS stain for cell glycogen.
	www.imagepath.com /stain.html (480 words)

	[No title] (Site not responding. Last check: 2007-10-31)
		Subsequently, Feulgen found that the mild acid hydrolysis of cellular DNA followed by the addition of a Schiff reagent provided a reddish-purple color to DNA-containing structures.
		Although the Feulgen thionin staining method developed in the 1950s does facilitate quantitative DNA measurements, the use of t-butanol as a solvent for the traditional thionin staining reagent is not without its drawbacks.
		M. In contrast to Feulgen staining solutions known in the art that have a molar ratio of metabisulfite: thionin of 4-10: 1, in the composition of the present invention, a molar metabisulfite: thionin ratio of 1: 1 is preferred.
	www.wipo.int /cgi-pct/guest/getbykey5?KEY=99/02960.990121&ELEMENT_SET=DECL (4468 words)

	Assay Quality Control (Site not responding. Last check: 2007-10-31)
		The instrument measures optical density of feulgen stained specimens at a wavelength of 620nm.
		A system performance evaluation is performed on calibration slides to verify that the CAS system is adjusted properly and that the Feulgen staining procedure was done correctly (Bacus et al 1994).
		All individual sputum collections from a subject will be analyzed within one Feulgen stain batch, and equal numbers of samples from treatment and control groups will be analyzed per batch as well.
	www.cpl.colostate.edu /morph/qc.htm (531 words)

	Feulgen staining of rat liver fixed in different fixatives. (Site not responding. Last check: 2007-10-31)
		Feulgen staining of rat liver fixed in different fixatives.
		Feulgen staining of rat liver fixed in different fixatives.The results of this study reveal that following hydrolysis for different time periods the tissue material fixed in formalin show the same staining pattern as those fixed in glutaraldehyde.
		The material fixed in Heidenhain's-Susa displays an intense Feulgen staining after two different times of hydrolysis, and that fixed in Flemming's fluid shows particular staining intensity for a prolonged time period thus indicating better preservation of DNA than in the materials fixed in the other three fixtatives.
	www.ihop-net.org /UniPub/iHOP/gp/2653843.html (142 words)

	BIOQUANT Image Analysis: Genome Size by Feulgen (Site not responding. Last check: 2007-10-31)
		As will be seen, Feulgen image analysis provides a rapid, cost-effective, and user-friendly method for the measurement of animal genome sizes.
		The problems discussed are those faced by Genome Size by Feulgen Image Analysis 747 all densitometric methodologies, but the speed and accuracy of image analysis allows many of them to be addressed directly in ways not previously possible.
		It is our hope that the existence of a fast, accurate, and cost-effective alternative to time-consuming densitometric and expensive fluorometric techniques will stimulate the collection of genome size data so that the profound gaps in the current genome database can be closed.
	www.bioquant.com /gallery/feulgen.html (1288 words)

	Preferential Staining of Nucleic Acid-Containing Structures For Electron Microscopy - H. E. HUXLEY, Ph.D., and G. ...
		The Feulgen method is highly specific for DNA, so the results show that the regions in the nucleus whose density is greatly increased by treatment with uranyl acetate are those which contain substantial amounts of DNA.
		To find out whether it is the DNA itself which is stained by uranyl acetate, or whether it is some other component associated with it, we have measured by direct weighing the uptake of stain by samples of purified DNA, of nucleohistone, and of histone, exposed to aqueous solutions of uranyl acetate of various concentrations.
		Finally, the Feulgen staining experiment indicates that at least a substantial part of the nucleic acid originally present in several tissues survives the preparative procedures and is available in the sections.
	www.mindfully.org /Nucs/Huxley-Zubay-Staining1nov61.htm (7524 words)

	ESACP DNA Consensus in Image Cytometry
		Specificity control of the Feulgen reaction may be performed by staining an unhydrolysed specimen which must remain unstained.
		Feulgen and Rossenbeck, 1924; Graumann, 1953; van Duijn, 1956; Kjellstrand, 1977; Larson and Sanaia, 1980; Krug, 1980; Schulte, 1986; Chatelain et al., 1989; Schulte and Wittekind, 1990, a/b; Schulte, 1991: Mikel and Becker.
		Schulte E, Wittekind D. Standardization of the Feulgen reaction: The influence of chromatin condensations on the kinetics of acid hydrolysis.
	www.biochem.mpg.de /valet/esacimag.html (2672 words)

	Untitled Document (Site not responding. Last check: 2007-10-31)
		The present analysis is concerned with this problem of a possibly variable genome size within the species or other reasons for this discrepancy.
		Internal standards used were Glycine max (flow cytometry, 2C taken as 2.226 pg) and Pisum sativum (Feulgen densitometry, 2C taken as 8.84 pg).
		Feulgen estimates were slightly higher but basically in agreement with these data.
	www.unifi.it /unifi/bioveg/Caryo/2053-6.html (216 words)

	[No title] (Site not responding. Last check: 2007-10-31)
		Terms like Feulgen stain and Feulgen reaction is presently the only staining technique which is stoichiometric for DNA, which means that staining of DNA is both quantitative and specific.
		The cells are first stained with an alkaline phosphatase technique including a monoclonal antibody specific to a protein in at least one of the cell's cytoplasm or on a cell membrane, thereby marking any cells including the protein as to type.
		A second staining of the DNA in the nucleus is accomplished by a Feulgen technique that destroys the cell cytoplasm.
	www.wipo.int /cgi-pct/guest/getbykey5?KEY=00/31534.000602&ELEMENT_SET=DECL (14194 words)

	NEL, Melatonin, GH and cardiac infarct
		To examine the cytoprotective effect of melatonin or recombinant human growth hormone (hGH) on the early phase of a running myocardial infarction in rats by using the Feulgen staining.
		The present study was undertaken to compare the cytoprotective effect of melatonin on the heart with that of recombinant human growth hormone (hGH), a treatment of demonstrable cytoprotective effectivity in experimental cardiac infarction [2,17,18].
		The early morphological changes occurring in the infarcted rat myocardium were assessed by Feulgen staining, a reaction which identifies early permeability changes of nuclear membrane resulting in the spilling of DNA into the cytoplasm [19,20].
	www.nel.edu /23_56/NEL235602A01_Castagnino_Cardinali.htm (612 words)

	Developmental Biology Cinema, Le Douarin (Site not responding. Last check: 2007-10-31)
		(The Feulgen stain, stains DNA.) Feulgen stained quail cells have interphase nuclei with condensed nucleolar-associated heterochromatin that's not found in chick interphase nuclei.
		To the right is an image of a Feulgen stained section showing quail cells on the left and chick cells on the right.
		Feulgen stained transverse section through the host chick embryo containing the grafted quail nerual tube and neural crest.
	www.sdbonline.org /archive/dbcinema/ledouarin/ledouarin.html (495 words)

	Abstrakts von Publikationen: Eva Maria Temsch (Site not responding. Last check: 2007-10-31)
		Despite of a huge amount of literature on the Feulgen reaction for DNA relatively few investigations deal with the special needs of plant scientists.
		In the present study, we re-evaluated recently published Feulgen densitometric data on genome size and its infraspecific variation in Arachis hypogaea, and also conducted measurements in one accession of its wild relative A. monticola.
		The methods applied were propidium iodide flow cytometry and Feulgen densitometry using Pisum sativum as an internal standard.
	www.botanik.univie.ac.at /pershome/temsch/abstrd.html (3985 words)

	New Products
		The Blue Feulgen DNA Ploidy Analysis Staining Kit is a histochemical reagent kit for quantifying the nuclear DNA content in cells.
		The basis of the Feulgen staining procedure is the use of an acid hydrolysis step to remove purines from the deoxyribose backbone of the DNA molecule.
		The newly exposed deoxyribose sugars have an aldehyde group, and this can then be identified by staining with a Schiff reagent (in this case, an aqueous solution of cresyl-violet and sulfurous acid used to test for the presence of aldehydes).
	www.scytek.com /catalog/contents/en-us/d51.html (1325 words)

	A CHROMATOGRAPHIC STUDY OF HYDROLYSIS IN THE FEULGEN NUCLEAL REACTION -- Woods 3 (1): 71 -- The Journal of Cell Biology
		A CHROMATOGRAPHIC STUDY OF HYDROLYSIS IN THE FEULGEN NUCLEAL REACTION -- Woods 3 (1): 71 -- The Journal of Cell Biology
		A CHROMATOGRAPHIC STUDY OF HYDROLYSIS IN THE FEULGEN NUCLEAL REACTION
		In a study on Feulgen hydrolysis of frozen-dried alcohol-fixed
	www.jcb.org /cgi/content/abstract/3/1/71 (291 words)

	FEULGEN NUCLEAL REACTION: I. QUANTITATIVE EXTRACTION OF FUCHSIN FROM FEULGEN-STAINED NUCLEOPROTEIN -- Hiraoka 3 (4): ...
		FEULGEN NUCLEAL REACTION: I. QUANTITATIVE EXTRACTION OF FUCHSIN FROM FEULGEN-STAINED NUCLEOPROTEIN -- Hiraoka 3 (4): 525 -- The Journal of Cell Biology
		From experiments utilizing this method, knowledge may be gained
		about the mechanism of the Feulgen reaction in situ which can
	www.jcb.org /cgi/content/abstract/3/4/525 (216 words)

	BGN 5: DNA content of dormant barley leaf nuclei (Site not responding. Last check: 2007-10-31)
		DNA content was determined by two-wavelength Feulgen cytophotometry following the procedure outlined by Pollister et al.
		The two wavelengths were selected from the Feulgen absorption curve of a homogeneous interphase nucleus.
		Approximately 7 arbitrary units of DNA was found to correspond to 2C and 14 units to 4C.
	wheat.pw.usda.gov /ggpages/bgn/5/5p6.html (540 words)

	RE: [Histonet] CK903 w/Feulgen (Site not responding. Last check: 2007-10-31)
		Carole -----Original Message----- From: Patti Loykasek [mailto:ploykasek@phenopath.com] Sent: Wednesday, March 16, 2005 3:59 PM To: Carole Fields Subject: Re: [Histonet] CK903 w/Feulgen Feulgen is not an antibody.
		It stains DNA by the negative PO4 groups of the DNA reacting with the positively charged nitrogen groups of the dye.
		If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided.
	www.histosearch.com /histonet/Mar05/RE.HistonetCK903w.Feulgen.html (302 words)

	Mitotic instability in callus cultures of inbred lines adapted to tropical regions
		Mitotic analysis was carried out on meristematic cells of proembryoids taken from the callus 5 days after its transferrence to fresh medium, fixed in 3:1 alcohol:glacial acetic acid and stained in Feulgen.
		These observations are consistent with the interpretation that the chromosome aberrations, usually involving knobbed chromosomes, observed in regenerants are the result of chromosome breakage occurring in culture (Phillips, RL et al., Corn and Corn Improvement, pp.
		Feulgen stained anaphase showing one bridge apparently derived from delayed segregation of sister chromatids.
	www.maizegdb.org /mnl/66/143aguiar-perecin.html (470 words)

	[Histonet] Feulgen stain for DNA (Site not responding. Last check: 2007-10-31)
		For Feulgens, Bouins may not be the best fixative particularly if you fix a long time in this solution.
		This is the very reason acid decalcifiers are not recommended if one wants to do Feulgens.
		When you then do Feulgens, HCL continues this hydrolyzation, the problem lies in overhydrolyzation, and poor staining.
	lists.utsouthwestern.edu /pipermail/histonet/2004-May/005817.html (140 words)

	Proliferating alveolar macrophages in BAL and lung function changes in interstitial lung disease -- Pforte et al. 6 ...
		This may be caused by recruitment of precursor cells from peripheral blood and/or local proliferation in the lung.
		In all patient groups there was a significant increase in proliferating AMs compared to healthy controls (4.2 versus 1.4% Feulgen, 2.1 versus 0.5% Ki67), with a significant correlation between these two parameters.
		Our main finding was, however, a positive correlation between numbers of proliferating cells (Feulgen) and lung function parameters, especially vital capacity and oxygen tension (PO2) at rest, in patients with sarcoidosis and lung fibrosis.
	erj.ersjournals.com /cgi/content/abstract/6/7/951 (363 words)

	Low-budgetary scheme for differentiation and DNA quantity investigation in blood lymphocytes of patients with chronical ... (Site not responding. Last check: 2007-10-31)
		The aim of the study is to reveal karyological and karyometrical aspects of chromatin distribution in chronization of the disease.
		(The quantitative study of cell DNA content and characteristic of cell cycle phases was carried out using cytophotometry of Feulgen reaction) After staining according to Feulgen, it was possible to determine the stage of vital cycle but if was difficult to determine the nucleus belonging to any type of the cells.
		Step-by-step discriminant analysis was used to assign pathological mononuclear cells stained by Feulgen method to the appropriate group of cells.
	www.iscb.org /ismb2003/posters/bvshilovAThotbox.ru_75.html (493 words)

	Analysis of Nuclear DNA Content in Capsicum (Solanaceae) by Flow Cytometry and Feulgen Densitometry -- MOSCONE et al. ...
		Key words: Solanaceae, Capsicum, genome size variation, flow cytometry, Feulgen densitometry, karyosystematics, karyotype evolution.
		Feulgen densitometry was performed following Greilhuber and Ebert (1994)
		Baranyi M, Greilhuber J. Flow cytometric and Feulgen densitometric analysis of genome size variation in Pisum.
	aob.oxfordjournals.org /cgi/content/full/92/1/21 (3962 words)

	Robert Joachim Feulgen Biography / Biography of Robert Joachim Feulgen World of Genetics Biography
		Although German physician Robert J. Feulgen published seventy research papers about the chemistry of physiology, histochemistry, and wrote a review of nucleic acid chemistry titled Chemie und Physiologie der Nucleinstoffe (Chemistry and Physiology of Nucleic Acids), he is best known for his discovery of a method for staining nucleic acid, now termed a Feulgen reaction.
		Feulgen was born in Werden, Germany, into a working class family, Feulgen paved his way to the medical school through an outstanding student performance that opened to him the doors of the University of Freiburg at Breisgau in 1905.
		His medical residency was taken in the City Hospital of Kiel, where he wrote his dissertation on the purine metabolism of patients with chronic gout.
	www.bookrags.com /biography-robert-joachim-feulgen-wog (234 words)

	Intraspecific Variation in Genome Size in Angiosperms: Identifying its Existence -- GREILHUBER 95 (1): 91 -- Annals of ...
		Influence of post-hydrolysis washes in distilled water at room temperature on Feulgen stainability of Pisum sativum meristems, fixed either with acetic methanol (MAA) or 4 % buffered formaldehyde (FA).
		Greilhuber J. Severely distorted Feulgen DNA amounts in Pinus (Coniferophytina) after nonadditive fixations as a result of meristematic self-tanning with vacuole contents.
		Feulgen densitometry: some observations relevant to best practice in quantitative nuclear DNA content determination.
	aob.oxfordjournals.org /cgi/content/full/95/1/91 (3963 words)

	Plant DNA C-Value Database Conference
		Many aspects of the protocols for estimating DNA C-values using Feulgen microdensitometry and flow cytometry were discussed.
		Feulgen stain: The workshop recommended that Feulgen stain for C-value estimations should always be prepared from basic fuchsin powder and not bought as a pre-prepared Feulgen solution.
		The staining capacity of ready-made solutions of Feulgen were shown to reduce over time (weeks) once the bottle had been open.
	www.rbgkew.org.uk /cval/pgsm (5206 words)

	TB29 - QUANTITATIVE MICROSCOPY OF FEULGEN STAINED BRONCHIAL BIOPSIES DETECTS LOSS OF HETEROZYGOSITY IN PRE-CANCEROUS ... (Site not responding. Last check: 2007-10-31)
		Quantitative Microscopy Of Feulgen Stained Bronchial Biopsies Detects Loss Of Heterozygosity In Pre-Cancerous Lesions.
		Image cytometry of Feulgen stained nuclei was performed.
		We correctly identified biopsies with LOH with 100% accuracy by discriminant function analysis.
	www.wiley.com /legacy/products/subject/life/cytometry/ISAC96/tb29.html (242 words)

	Plant Chromosomes (Site not responding. Last check: 2007-10-31)
		Stain with Feulgen stain: Remove HCl with Pasteur pipet, discard in running cold water.
		Add 1 mL Feulgen stain (caution: this stain does not look brightly colored, but stains strongly.
		Remove the Feulgen stain with a Pasteur pipet, discard into the drain.
	biology.clc.uc.edu /Fankhauser/Labs/Genetics/Root_tip_chromosomes/Vis_chrmja0.html (485 words)

	Cardinali (Site not responding. Last check: 2007-10-31)
		Cytoprotection by melatonin and growth hormone in early rat myocardial infarction as revealed by Feulgen DNA staining.
		OBJECTIVE: To examine the cytoprotective effect of melatonin or recombinant human growth hormone (hGH) on the early phase of a running myocardial infarction in rats by using the Feulgen staining.
		RESULTS: Infarcted rats receiving vehicle exhibited large, diffuse cardiac lesions with a marked positivity for Feulgen reaction.
	www.fmed.uba.ar /depto/fisiologia/neurosci/326.htm (253 words)

	ANNALS Dec1997: ALGORITHM ANALYSIS OF LECTIN GLYCOHISTOCHEMISTRY AND FEULGEN CYTOMETRY FOR A NEW . . . (Site not responding. Last check: 2007-10-31)
		ANNALS Dec1997: ALGORITHM ANALYSIS OF LECTIN GLYCOHISTOCHEMISTRY AND FEULGEN CYTOMETRY FOR A NEW.
		The data so obtained show that our morphologic classification of nasal polyps fits in with the classification of nasal polyps defined on the basis of clinical criteria.
		Keywords:diagnosis, Feulgen stain, glycohistochemistry, image cytometry, lectins, nasal polyps.
	www.annals.com /abs/annals94.htm (245 words)

	Scanning Feulgen-deoxyribonucleic acid cytophotometry of Papanicolaou destained preparations -- Anthony et al. 25 (4): ...
		Articles by Anthony, A. Articles by Romanosky, A. Scanning Feulgen-deoxyribonucleic acid cytophotometry of Papanicolaou destained preparations
		specimens revealed a differential loss in Feulgen reactivity among human
		lesions was observed to result in underestimates of relative Feulgen
	www.jhc.org /cgi/content/abstract/25/4/287 (157 words)

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