Where results make sense

About us   |   Why use us?   |   Reviews   |   PR   |   Contact us

Tutorial : Miniaturized Filtration Assay for Discovery Genetic Engineering News - Biotechnology from Bench to Business Binding in the presence of excess, unlabeled ligand is used to determine assay nonspecific binding, which is then subtracted from total binding (absence of competing ligand) to calculate specific binding. Saturation-binding assays are ideal for the determination of the binding affinity that the receptor has for a ligand and for determining the specific activity of the receptor in a given receptor preparation. Filter plate and assay selection is based on the nature of the substrate molecule to be phosphorylated (e.g., a short synthesized peptide or a native protein). www.genengnews.com /articles/chtitem.aspx?tid=1095&chid=1   (1516 words)

Assay device and use thereof - Patent 6812038 An assay method for determining an analyte in a sample wherein the sample and assay liquids are flown through a flow matrix to reach the reaction zone in a predetermined sequence uses the device for carrying out the method. An object of the present invention is to provide an assay device for conducting an assay for the determination of an analyte, which device permits simultaneous initiation of flow of sample and at least one other assay liquid. The assay is then started by the operator removing the pull-out film 5 to thereby put the membrane strip 6 in simultaneous liquid receiving contact with the buffer pad 12 and the sample liquid in the sample well 3. www.freepatentsonline.com /6812038.html   (3543 words)

Filter (chemistry) - Wikipedia, the free encyclopedia In chemistry and common usage, a filter is a device (usually a membrane or layer) that is designed to block certain objects or substances while letting others through. Filters are often used to remove harmful substances from air or water, for example to remove air pollution, to make water drinkable, to prepare coffee. Filtrate is the liquid produced after filtering a suspension of a solid in a liquid. en.wikipedia.org /wiki/Filter_(chemistry)   (119 words)

Protein-DNA interactions For instance, Cro binds tightly to oR3 and weakly to oR1 and oR2; CI binds tightly (and cooperatively) to oR1 and oR2, weakly to oR3. One carries out a series of binding reactions over a range of protein concentrations, and determines the fraction of the binding site that is occupied at each protein concentration. Result: DNA molecules to which proteins bind move more slowly in the gel and are retarded relative to the sample with no protein. www.biochem.arizona.edu /classes/bioc568/protein_dna_interactions.htm   (1973 words)

Assay Encyclopedia   (Site not responding. Last check: ) There are numerous types of assays, such as an antigen capture assay, bioassay, competitive protein binding assay, four-point assay, immunoassay, microbiological assay, stem cell assay, and many others. Assays are regularly utilized in molecular biology scientific research laboratories. Viral plaque assay: Used to calculate the number of viruses present in a sample. www.hallencyclopedia.com /topic/Assay.html   (395 words)

Assay - Wikipedia, the free encyclopedia An assay is a procedure where the concentration of a component part of a mixture is determined. There are numerous applications of an assay, such as an antigen capture assay, bioassay, competitive protein binding assay, four-point assay, immunoassay, microbiological assay, stem cell assay, and many others. Assays are regularly utilized in scientific research laboratories. en.wikipedia.org /wiki/Assay   (285 words)

Multi-well Filter Plate Glossary of Terms Adenine binds with thymine in DNA and with uracil in RNA. ASSAY A method/procedure for detecting the presence, estimating the concentration, and determining the biological activity of a macromolecule, molecule, ion, or cell. It is commonly used to prevent antibodies or DNA from binding directly to a membrane instead of binding to a desired biomolecule. www.pall.com /34696_26868.asp   (4145 words)

Fluorescence Polarization Method To Characterize Macrolide-Ribosome Interactions -- Yan et al. 49 (8): 3367 -- ... Equilibrium binding of BODIPY-erythromycin to bacterial ribosomes in an FP assay. Fluorescent assay for estimating the binding of erythromycin derivatives to ribosomes. Kinetics of binding of macrolides, lincosamides, and synergimycins to ribosomes. aac.asm.org /cgi/content/full/49/8/3367   (3046 words)

Development of a Fluorescent Ligand-Binding Assay Using the AcroWell™ Filter Plate Representative plots from saturation binding curves (A and B) and Scatchard transformations (C and D) are shown for the TRF and radioactive assay formats. Saturation binding experiments were conducted with 0.012-6 nM europium-labeled galanin in the absence and presence of the indicated concentrations of DMSO Nonspecific binding was determined in the presence of 1.0 µM unlabeled galanin (Sigma Chemical Co., St. Louis, MO). To fully characterize the use of filter plate assays for analysis of active compounds identified by high throughput screening and for the quantitation of receptor-ligand interactions, dose-displacement assays were conducted using both unlabeled galanin and P5718972 as competitors (Fig. www.pall.com /34696_1186.asp   (3405 words)

Millipore - Technical Library - Kinase Assays Performed Entirely in the MultiScreen® HTS–PH 384-well ... Filter binding is an important tool in the screening of compounds which affect kinase activity. Assay performance of reactions performed entirely in the filter plate (in-plate assay) is equivalent to reactions carried out in a separate reaction plate (out-of-plate assay). 384-well filter plate enhances throughput of enzymatic assays by providing a high density platform filter binding assay in which all the steps of the reaction can be performed directly in the filter plate. www.millipore.com /publications.nsf/docs/an1017en00   (1371 words)

Reed Laboratories - Mineral Analysis, Ore Grade, Prices Silver - Assay of rocks, soils, drill core, or pan concentrates for Ag. Fire assay is the standard test for precious metals, reporting Au. Fire assay or fusion/acid extractions, and xray spectrometer or ICP finish, reporting troy oz. home.nethere.com /rely/price.html   (1257 words)

BioMed Central | Full text | DNA unwinding assay using streptavidin-bound oligonucleotides When streptavidin binds to the substrate shown in Figure 1F it functions in a manner similar to forked substrate presumably by excluding one strand from the helicase [12]. As shown in Figure 2 the binding of streptavidin to the substrate substantially improves helicase activity of all three enzymes (Fig, 2A and 2B compare IV to II and C compare III to I, see also D-F). For assays involving biotin-streptavidin complexes, 10 fmol of biotinylated substrate was incubated with 1 pmol of streptavidin for 10 min at 30°C prior to use. www.biomedcentral.com /1471-2199/7/43   (3179 words)

MIT OpenCourseWare | Biology | 7.28 Molecular Biology, Spring 2005 | Study Materials Assay for directionality of helicase movement; used to study function of replication termination sequences. Assay for homologous DNA pairing and branch migration. Assay for whether a protein bends DNA upon binding and position of bend. ocw.mit.edu /OcwWeb/Biology/7-28Spring-2005/StudyMaterials/index.htm   (486 words)

Characterization of the DNA-binding activity of HIV-1 integrase using a filter binding assay. Characterization of the DNA-binding activity of HIV-1 integrase using a filter binding assay. Based on the selective binding of proteins and DNA to distinct filter materials a double-layered dot blot radio assay was developed to evaluate the binding of DNA to HIV-1 integrase. In this assay the DNA-binding was found to be independent of Mn2+ concentration, inhibited by concentrations of Mg2+ above 5 mM, abolished by zinc chelation and inhibited by monoclonal antibodies reacting with either the N-terminal or C-terminal regions of integrase. www.aegis.com /aidsline/1996/apr/M9640036.html   (381 words)

FAQ Both assays (Caco-2 and PAMPA) revealing in the first experiment Peff as their output, which quite suitable to estimate the permeation of pharmaceutical compounds through the human GI tract. Nevertheless, if the compounds are suspected to show unspecific binding to the filter membrane during the assay, the centrifugation route should be preferred. Describes the ability of compound to bind to plasma proteins, like serum albumin, Éø1-acid glycoprotein and lipoproteins or to blood cells which influences the disposition and efficacy of a compound in vivo. www.nimbus-biotech.com /menue_faq.html   (2227 words)

J. Biochem. 131, pp. 541-546 (2002)[body text] The binding of ATP to Orc1p, one of the ORC subunits, is essential for ORC-binding to origin DNA (13). We have previously identified basic amino acid residues in DnaA that are essential for its binding to CL, and proposed that the ionic interaction between DnaA and CL changes the conformation of DnaA, resulting in a decrease in the affinity of DnaA for ATP or origin DNA (21-25). The inhibitory effect of cardiolipin on ORC binding to origin DNA was suppressed by low incubation temperatures or the addition of chlorpromazine, suggesting that the inhibitory effect of phospholipids on ORC binding to origin DNA requires membrane fluidity. wwwsoc.nii.ac.jp /jbiochem/jb/131-4/4fbauvtx.htm   (2656 words)

Filter-Binding Assay for Covalent DNA-Protein Complexes: Adenovirus DNA-Terminal Protein Complex -- Coombs and Pearson ... A rapid, simple, and quantitative filter-binding assay using glass fiber filters has been developed to detect the covalent adenovirus DNA-terminal protein complex. The assay is unusually sensitive because binding of protein-free DNA generally is less than 0.1%. Binding of the adenovirus complex to filters is mediated by terminal protein. www.pnas.org /cgi/content/short/75/11/5291   (196 words)

The RNA Binding Activity of a Ribosome Biogenesis Factor, Nucleophosmin/B23, Is Modulated by Phosphorylation with a ... The RNA Binding Activity of a Ribosome Biogenesis Factor, Nucleophosmin/B23, Is Modulated by Phosphorylation with a Cell Cycle-dependent Kinase and by Association with Its Subtype -- Okuwaki et al. The RNA Binding Activity of a Ribosome Biogenesis Factor, Nucleophosmin/B23, Is Modulated by Phosphorylation with a Cell Cycle-dependent Kinase and by Association with Its Subtype The efficiency of the RNA binding activity of the B23 complexes as a function of the ratio of B23.2 to B23.1 is calculated and summarized at the right plot. www.molbiolcell.org /cgi/content/full/13/6/2016   (8829 words)

Assay   (Site not responding. Last check: ) The number of viruses present in a sample can be determined using a viral plaque assay. Precious metals, platinum, gold and silver, used in jewellery, silverware and other items, are assayed to test the purity of the metal. Once an item has been assayed, it is usually hallmarked to the relevant standard. www.dejavu.org /cgi-bin/get.cgi?ver=93&url=http%3A%2F%2Farticles.gourt.com%2F%3Farticle%3Dassay%26type%3Den   (280 words)

The Tomato R Gene Products I-2 and Mi-1 Are Functional ATP Binding Proteins with ATPase Activity -- Tameling et al. 14 ... The radioactivity bound to the proteins was measured in a filter binding assay as described in Methods. of a kinase-2 motif in the NBS of I-2, nucleotide binding is P-ATP binding was measured in a filter binding assay as described in Methods. www.plantcell.org /cgi/content/full/14/11/2929   (5036 words)

BioMed Central | Full text | Specificity of DNA triple helix formation analyzed by a FRET assay This assay is homogeneous, continuous and specific, because the appearance of the FRET signal is directly correlated to triplex formation. The fluorescence of the TAMRA group was analyzed with respect to the equilibrium binding constants of the TFOs to the corresponding DNA. This result confirms that the TFO binds to the DNA in an antiparallel orientation with respect to the polypurine strand [5,25], because in a parallel orientation the distance between the donor and acceptor groups would not allow highly efficient FRET. www.biomedcentral.com /1471-2091/3/27   (2775 words)

Nucleic acid binding properties of the nucleic acid chaperone domain of hepatitis delta antigen -- Wang et al. 31 (22): ... A 25 µl reaction mixture in standard binding buffer containing 0.5 or 1 nM of PRP19S and the indicated concentration of NdAg was incubated at 37°C for 10 min in a 1.5 ml tube. The filter binding assay was carried out in the presence of 0.5 nM PRP19S, 5 nM KSR4 and 0–10 µM of protein in standard binding buffer at 37°C for 30 min. The NdAg binding curves of the group II poly rU that had an average size of 155 nt and the group IV poly rU obtained at 37°C in standard binding buffer (open circle), or with NaCl added to 0.2 M (filled circle), 0.3 M (open square), 0.4 M (filled square) or 0.5 M (open triangle). nar.oxfordjournals.org /cgi/content/full/31/22/6481   (7021 words)

CytoDYNAMIX Screens™ There is a continual improvement and novel design process of HTS assays at Cytoskeleton, Inc. if you have an interest in one of these assays or you need an assay that you do not see here please contact our technical support. There is a radioactive filter based endpoint assay (BK055) with 10 nM detection limit that is good for small G-proteins and microtubule polymerizations, which have low GTPase activity (Kcat <0.01). If you are interested in assaying a specific tubulin not listed on our website, please contact our technical support to discuss how we can provide you with the specific tubulin type you want. www.cytoskeleton.com /products/hts/hts.html   (1239 words)

HIV Vif as a Therapeutic Target The immediate purpose of the workshop was to review assays currently available to screen for inhibitors of HIV-1 Rev, debate their relative strengths and weaknesses, and identify those that can be adapted for high-throughput screening (HTS) in the DAIDS HIV drug discovery and development program. Assembly of the three components forms an immobilized ternary complex whereby the biotinylated RRE binds to the streptavidin, and the fluorescein-tagged Rev binds to the RRE. Attention is also given to design parameters that permit interchangeable porting of assays from HTS platforms to the laboratory bench, thereby broadening use and application of the final assays to academic laboratories. www.niaid.nih.gov /daids/hivrev.htm   (1838 words)

Control of Ribosomal Protein L1 Synthesis in Mesophilic and Thermophilic Archaea -- Kraft et al. 152 (4): 1363 -- ... The results of these binding studies are shown in Figure 2. Values of RNA retained on the filter in the absence of protein (2–5% for the rRNA fragments and 7.5–10% for the mRNA fragments) were subtracted before the data were blotted. Values of RNA retained on the filter in the absence of protein (2–5%) were subtracted before the data were blotted. www.genetics.org /cgi/content/full/152/4/1363   (4619 words)

Luminometer Grants Program Recipients from Turner BioSystems One example is to study the efficiency of gene carriers administered to mucus covered lung epithelial cells for cystic fibrosis, where the thick mucus layer is a formidable barrier for gene therapy. The luciferase assay represents an important complementary tool to our current green fluorescence protein (GFP) based reporter protein, because it allows us to standardize our results to the luciferase assay commonly used in the field, and facilitate much higher throughput of in vivo testing due to auto-fluorescence in animals. His goal is to characterize the effect of PGF2and#61537 on cyp19 expression and to investigate in vivo transcription factors which may regulate basal expression of P450arom and to identify those that may mediate the effect of PGF2a on cyp19 transcription. www.luminometer.org /luminometer-recipients.html   (4442 words)

RNA-binding activity of translation initiation factor eIF4G1 from Saccharomyces cerevisiae -- BERSET et al. 9 (7): 871 ... Binding of eIF4G1 and eIF4G1 fragments to poly(U) agarose. Purified GST-eIF4G1 fusion proteins were bound to poly(U) agarose for 30 min at 4°C, the resin washed extensively with buffer, proteins bound to the matrix eluted by boiling with SDS sample buffer, fractionated on 17.5% SDS polyacrylamide gels, and stained with Coomassie blue. for RNA binding in vitro in the filter-binding assay and approximate www.rnajournal.org /cgi/content/full/9/7/871   (4972 words)

High-affinity, Non-sequence-specific RNA Binding by the Open Reading Frame 1 (ORF1) Protein from Long Interspersed ... The binding affinity of ORF1p to RNA was unaffected by EDTA concentrations between 0 and 50 m Nitrocellulose filter binding assays were performed in binding buffer containing different concentrations of NaCl, using the sense transcripts of 110 nt at100 p Binding of ORF1p to sense and antisense 40-nt transcripts. www.jbc.org /cgi/content/full/278/10/8112   (4583 words)

Roles of Adeno-Associated Virus Rep Protein and Human Chromosome 19 in Site-Specific Recombination -- Young et al. 74 ... Filter-binding assays were performed as described by Fuller and his colleagues (11), with some modifications. Filters were then exposed to the phosphorimager cassette and the relative intensity was measured. Lane 1, 10 µg of XbaI-digested ch-19 (PMEF) DNA fractionated on agarose gel, transferred to membrane, and hybridized with a ch-19 sequence-specific probe; lane 2, 10 µg of XbaI-digested ch-19 PMEF DNA was fractionated on agarose, transferred by Southern blotting, and hybridized with a Neo sequence-specific probe. jvi.asm.org /cgi/content/full/74/9/3953   (9076 words)

Try your search on: Qwika (all wikis)

About us   |   Why use us?   |   Reviews   |   Press   |   Contact us   Copyright © 2005-2007 www.factbites.com Usage implies agreement with terms.