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Topic: Filter fluorometer


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  Filter (optics) - Wikipedia, the free encyclopedia
Coating-based filters are often referred to as dichroic, and can be used in devices such as a dichroic prism to separate a beam of light into different coloured components.
Neutral density (ND) filters have a constant attenuation across the range of visible wavelengths, and are used to reduce the intensity of light by reflecting or absorbing a portion of it.
Light from a clear blue sky is also polarized, and adjustable filters are used in colour photography to darken the appearance of the sky without introducing colours to other objects, and in both color and fl-and-white photography to control specular reflections from objects and water.
en.wikipedia.org /wiki/Filter_(optics)   (799 words)

  
 Tutorial - Interference Filters: C&L Instruments, Inc.   (Site not responding. Last check: 2007-11-03)
To complete the interference filter, another set of thin-film coatings are applied to the second substrate to block the transmission of wavelengths that are further away from the passband of interest.
The ability of a bandpass interference filter to discriminate between in-band and out-of-band wavelengths is a function of the number of cavities employed in the design of the filter.
Note that as the number of cavities used in the construction of the filter is increased, the degree of attenuation of light in the out-of-band is greater at wavelengths further away from the center wavelength.
www.fluorescence.com /tutorial/int-filt.htm   (2152 words)

  
 Filter fluorometer - Wikipedia, the free encyclopedia
A filter fluorometer is a type of fluorometer that may be employed in fluorescence spectroscopy.
The primary filter or excitation filter or incident light filter isolates the wavelength that will cause the compound to fluoresce (the incident light).
The proper selection of filters requires familiarity with the emission spectrum (fluorescent spectroscopy) and the excitation spectrum.
en.wikipedia.org /wiki/Filter_fluorometer   (116 words)

  
 The Filter Fluorometer
The filter fluorometer is a device that exploits some of the principles of fluorescence in order to identify and quantify fluorescent molecules (dyes).
A filter fluorometer exploits this fact to identify one fluorescent molecules within a sample that may contain many several fluorescent molecules.
Light emitted from the fluorescent molecules that is travelling orthogonal to the excitation light beam pass through a secondary filter that removes most of the light outside of the target wavelength range.
www.dyetracing.com /dyetracing/dy01003.html   (380 words)

  
 Default Template Version 13   (Site not responding. Last check: 2007-11-03)
Filter fluorometers can be calibrated by a number of different techniques.
The most common calibration of a filter fluorometer consists of compensating for blank (solution containing zero concentration of the substance to be read) and adjusting the instrument to reflect a known concentration of sample (the standard).
Filter fluorometers can also be calibrated by correlating their raw fluorescence signal compared to a standard laboratory method like EPA method 413.1.
www.turnerbiosystems.com /doc/appnotes/998_0050/0050_c5.html   (1190 words)

  
 Fluorometric Detection of Optical Brighteners as an Indicator of Human Sources of Water Pollution. Part II. Development ...
The fluorometer can be used in the lab or field to measure small volumes in a cuvette (as described in Part I), or equipped for field use where water is continually pumped through the instrument with a submersible pump, and results from a digital readout are obtained immediately.
The initial fluorometer tests were inconclusive as many of the shellfish beds were away from shore and located near or in the main channel.
The fluorometer results confirmed the human signature results obtained from MST, and demonstrated the utility of the fluorometer, where the presence (or absence) of a human signature could be obtained instantly, as compared to two weeks or more for MST results.
www.ext.vt.edu /news/periodicals/cses/2005-11/part2.html   (4087 words)

  
 Science Section:PMT
The fluorometer measures the amount of fluorescent radiation produced by a sample when the sample is exposed to monochromatic light.
In early fluorometers, fluorescent light entered the vacuum phototube through the secondary filter at right angles to the exciting light.
The photocurrent is recorded by an electric amplifier connected to a galvanometer, which detects and measures a small electric current by movements of a magnetic needle of coil in a magnetic field.
history.nih.gov /exhibits/bowman/ScienPMT.html   (301 words)

  
 Used Sequoia-Turner 450-000 Filter-type Fluorometer for sale by LABEQUIP: scientific instruments and lab equipment of ...
The Turner 450 fluorometer is a compact instrument whose applications include detection/ quantitation of DNA, RNA, proteins, histamines, aflatoxins, chlorophill & ATP plus fluorescent tags (eg.
Filters are not included, but may be availalbe new.
The appearance of those names is not intended to imply that Labequip is an officially authorised dealer or distributor for those manufacturers (although that may occasionally be the case).
www.labequip.com /catalog.php?stkno=24073   (244 words)

  
 AIMS Research - Water Quality, Field and Analytical Procedures - Standard Operational Procedure 6, Contents
Filter papers are ground in 90% acetone (V/V) and centrifuged to extract the chlorophyll pigments.
The fluorometer is standardised spectrophotometrically (Jeffery and Humphrey 1975) against extracts of pigments from exponentially growing cultures of the diatom Chaetoceros simplex (chlorophylls a and c).
The weight difference between filter papers before and after filtration and drying is used to calculate the amount of suspended solid in the sample.
www.aims.gov.au /pages/research/reef-monitoring/ltm/mon-sop6/sop6-00.html   (5734 words)

  
 [No title]
The filter fluorometer in this experiment uses a longwave ultraviolet (UV) lamp with a primary bandpass filter, centered at 360 nm, located between the UV source and the sample.
A secondary cut-off filter, located between the sample and the detector, only allows wavelengths longer than 460 nm to pass.
Filter rapidly through filter paper, catching the filtrate in a 100 mL volumetric flask.
www.sou.edu /chem/ch425/Current426Experiments/salicylicacidfluorescence_2005.doc   (1009 words)

  
 C&L's Design Advantage   (Site not responding. Last check: 2007-11-03)
Our unique technology allows for two filter wheels (excitation and emission) to be precisely phase-synchronized at variable speeds, up to 7500 RPM, allowing continuous measurements at rates up to 1,000 data points per second at eight different wavelengths.
Optically, interference filter-based fluorometers are preferable to monochromator-based fluorometers.
The cost of a filter, which should never need to be replaced, is often less than the cost of the fluorescent probes used in an experiment.
www.fluorescence.com /products/design.htm   (791 words)

  
 Turner Designs TD-700 Laboratory Fluorometer   (Site not responding. Last check: 2007-11-03)
The TD-700 Fluorometer is a compact, easy-to-use benchtop instrument equipped with a multi-assay filter cylinder and multi-point calibration capability.
The TD-700 Fluorometer is a compact, easy-to-use benchtop instrument equipped with a multi-assay filter cylinder and multi-point calibration.
The TD-700 Laboratory Fluorometer has a multi-assay filter cylinder which allows it to be easily configured for GFP quantitation as well as other fluorescence assays.
www.turnerdesigns.com /t2/press_rel/press_rel_td700.html   (2237 words)

  
 Filter Fluorometer Facts
Thus, it is very important to select correct optical filters, low noise PMT tubes, and a solvent with low fluorescence response of it's own.
Lasers do not require excitation filters since emission wavelengths are not present in their output spectrum.
14) Interference filters are recommended to obtain maximum fluorescence to solvent signal ratio.
home.flash.net /~otd/filter2.htm   (558 words)

  
 Fluorometer Grants Program from Turner BioSystems
The TBS-380 Mini Fluorometer is a sensitive, compact benchtop fluorometer that is ideal for measuring DNA, RNA and proteins.
Using the optional Minicell Adaptor, this fluorometer can detect as little as 50 pg of DNA while only using a sample volume of 50 µL. These small assay volumes save precious samples and expensive reagents.
The Picofluor Fluorometer is a portable, battery-powered fluorometer that features high sensitivity, data logging, and two different fluorescence channels in a compact package.
www.fluorometer-grants.org /fluorometer-offered.html   (167 words)

  
 Fluorometer Quantitation of Nucleic Acids   (Site not responding. Last check: 2007-11-03)
The fluorometer shines light on the sample (excitation) and then measues level of fluorescent light being emitted to the side (at a 90º angle) away from the excitation light beam.
Do not use glass (spectrophotmetry) cuvettes in a fluorometer because the frosted glass on the side of the cuvette interferes with detection of fluorescent light.
Insert the correct LED and filter set in the fluorometer for either RNA or DNA.
www.fhcrc.org /science/labs/fero/Protocols/Fluorometer.html   (490 words)

  
 ADC BioScientific :: Products :: Environmental   (Site not responding. Last check: 2007-11-03)
Our environmental fluorometers are also currently being used in a host ecophysiological experimentation, from monitoring the marine and freshwater environment to tracking genetically modified organisms.
The GFL-1 is the first truly portable, battery operated filter fluorometer for the continuous or discrete analysis of environmental samples.
The GFP meter is a self contained, probe-based fluorometer that utilises a modulated detection system to compensate for stray ambient light and signal drift associated with changes in temperature and battery voltage.
www.adc.co.uk /products/environmental/index.htm   (434 words)

  
 The Synchronously Scanning Spectrofluorophotometer   (Site not responding. Last check: 2007-11-03)
The synchronously scanning spectrofluorophotometer (SSS), while still exploiting the same principles of fluorescence as the filter fluorometer, did not need special filters to remove unwanted wavelengths of light from the excitation beam.
Fixed excitation/variable emission analysis -- For this type of analysis, the excitation beam is set to a particular wavelength and the emission monocromator is set to scan through a defined wavelength range measuring the intensity of any light emitted from the sample.
While filter fluorometers are still manufactured and still have their appropriate uses, the SSS has necessarily become the standard for fluorescence analysis.
www.dyetracing.com /dyetracing/dy01004.html   (531 words)

  
 Fluorometer addition   (Site not responding. Last check: 2007-11-03)
SCAMP's fluorometer consists of a high intensity blue L.E.D. and a 455 nm interference filter that illuminate a small sample volume of water.
The fluorometer's optical components are mounted in a special anodized aluminum sensor endcap, shown in the picture below.
The fluorometer is sampled at the full 100 Hz rate so resolution at 10 cm/sec is 1 mm.
www.pme.com /fluoro.htm   (551 words)

  
 Comparison of spectrophotometer estimates of chlorophylls-a, -b, -c and ‘pheopigments’ in Florida Bay seston with ...
Unfortunately, a routine filter fluorometer was not available and coincident fluorometric data are lacking.
Storage and transport of the aluminum foil wrapped quarter folded filters was on dry-ice.
The UV/Vis spectrum of an additional 1.0 mL aliquot of the filtered raw extract was recorded and instrument derived absorption values recorded at 630, 645, 647, 663, 664, 665 and 750 nm for use in the polychromatic equations to be tested.
www.aoml.noaa.gov /ocd/sferpm/louda/louda_chl_compare.html   (3170 words)

  
 McPherson New Release - 4 Channel Fluorescence Detection and Fluorometer
The McPherson fluorometer gathers data for atmospheric photochemistry and facilitates better understanding of the general effects of pollution on climate and air quality.
The McPherson filter fluorometer simultaneously detects fluorescence emission of four unique samples.
The McPherson four channel filter fluorometer is useful for many applications.
www.mcphersoninc.com /pressreleases/quadfluoro.htm   (264 words)

  
 Filters - Accessories - Products - ISS
While the filter wheel can be utilized with any experiment, it is ideal for experiments which do not allow for changes in samples, its position or orientation.
ISS provides a variety of filters whether you need an optical density, low pass, band pass or high pass filter.
Shown here clockwise, are the holders for the filter wheel, a sample chamber and for the 3-position sample chamber.
www.iss.com /products/accessories/filters/index.html   (206 words)

  
 Turner BioSystems TD-700 Laboratory Fluorometer Press Releases
November 1996: Turner BioSystems Inc., specialists in filter fluorometry for over 20 years, and CLONTECH Laboratories Inc., leading supplier of innovative tools for life science research, are pleased to offer researchers a simple and accurate method for Green Fluorescent Protein (GFP) quantitation.
September 1996: The Turner BioSystems TD-700 Laboratory Fluorometer quickly and accurately quantitates Aequorea victoria Green Fluorescent Protein (GFP), a fluorescent marker used to monitor gene expression in a variety of organisms.
March 1996: Using Turner BioSystems' TD-700 Laboratory Fluorometer and Molecular Probes' PicoGreen® Assay, ds DNA can be quantitated as low as 25 picograms/ml, even in the presence of RNA, proteins, and nucleotides.
www.turnerbiosystems.com /press_rel/press_rel_td700.php   (1664 words)

  
 Data Sheet on the TD-700 Laboratory Fluorometer: A versatile instrument for environmental and biotechnology applications
The TD-700 Laboratory Fluorometer is a compact, low-cost benchtop fluorometer that features a unique multi-assay filter cylinder, wide wavelength range, menu-driven software, and multi-point calibration.
Lift the TD-700's sample chamber door and the unique filter cylinder slips out easily; it is ported to allow four different filter combinations.
Eight one-inch round filters (four excitation and four emission) are easily inserted.
www.navarc.com /material/700/998_7020.htm   (624 words)

  
 Ammonium Concentration Determination Optical Kits for Fluorometers
Affordable: Filter Fluorometer with optical kit and one working reagent.
The Ammonium Optical Kit (P/N 10-303) includes two Near UV Mercury Vapor Lamps, 350nm excitation filter (310-390nm), a 410-600nm emission filter, a 1:75 Attenuator plate, and a 10-300 Reference Filter (>300nm).
The 10-303 Optical Kit works in the 10-AU Digital Field Fluorometer, TD-700 Laboratory Fluorometer and the Model 10 Analog Fluorometer and also includes a reference filter (P/N 10-300).
www.turnerdesigns.com /t2/doc/appnotes/s_0025.html   (317 words)

  
 North Temperate Lakes Long Term Ecological Research   (Site not responding. Last check: 2007-11-03)
These are polycarbonate track-etched 0.4 um membrane filters used to filter out solids in order to measure various dissolved chemistries
These are pre-weighed polycarbonate track-etched 0.4 um membrane filters used to measure total particulate matter.
Note that State Lab of Hygeine chlorophyll samples have been taken in the past but are currently not taken due to poor comparison with the DNR spectrophotometer results.
limnosun.limnology.wisc.edu /sl_filters.shtml   (332 words)

  
 Molecular Fluorescence
The instrument used in this experiment uses glass filters (rather than some type of expensive grating or prism monochromator) to isolate the appropriate excitation and fluorescence wavelengths.
Excitation Wavelength Selection: A narrow-band 360-nm filter with a bandpass of 40 nm.
The instrument asks for the number of points for the calibration curve; use the up arrow to increase the number to 5.
www.chem.uky.edu /Courses/che226/labs/molecular_fluorescence.html   (771 words)

  
 FLUORAT®-02-series Analyzers
-02, is a filter fluorometer, chemiluminometer and photometer implemented together in the same optoelectronic scheme, whereas the FLUORAT
-02-2M version is, in addition, a time-resolving fluorometer and can also be used as a fluorometric detector in any HPLC system, relevant measuring cells being interchangeable.
The instrument is basically a fluorometer, however, it can be also operated as a photometer, chemiluminometer, or phosphorimeter.
www.spectradyntech.com /products/fluorat.htm   (542 words)

  
 ARS | Publication request: Design of a Portable Fluorometer Capable of Time-Resolved Luminescence Measurements   (Site not responding. Last check: 2007-11-03)
Citation: Chen, G. Design of a portable fluorometer capable of time-resolved luminescence measurements.
Technical Abstract: A rugged, filter-based fluorometer was developed for time-resolved luminescence (TRL) measurements in the field.
Several accessories were also built to support versatile measurement modes in liquid and solid phases, and in evanescent field.
www.ars.usda.gov /research/publications/publications.htm?SEQ_NO_115=163937   (222 words)

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