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| | HannerWebster |
 | | Freezing remains the most reliable and versatile method of preserving tissue for long-term storage (Engstrom, et al.,1999), while ultracold storage of biological tissues preserves a broad suite of biochemical characteristics (Cook, et al., 1999), although protein and lipid changes can occur during frozen storage (Florian, 1990). |
 | | The objective is to achieve a controlled rate of freezing, as opposed to the common practice of flash freezing specimens in liquid Nitrogen. |
 | | Then, simply place the Freezing container loaded with cryovials of specimens into the cold cryoshipper, close the lid on the cryoshipper and let the specimens equilibrate for at least three hours. |
| www.ou.edu /journals/dis/DIS84/Tec10%20Hanner/Hanner.htm (534 words) |
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