
	Where results make sense

Topic: Flow immunophenotyping

Related Topics

David Manning

Sabre Airline Reservations System

Ratan Tata

In the News (Sat 2 Jun 12)

EXECUTIVE POWER

Iran

ANALYSIS

Pakistan

Family compact

	Encyclopedia: Flow immunophenotyping (Site not responding. Last check: 2007-11-06)
		Flow immunophenotyping is a method used to analyze blood, bone marrow or other tissues in order to determine the expression level of certain proteins on the component cells.
		Cell suspensions are mixed with a cocktail of fluorescently-labeled antibodies and then analyzed, one cell at a time, using flow cytometry.
		Briefly, a laser light is used to probe the individual cells in order to calculate their size (forward scatter), internal complexity (side scatter) and the presence of the antigen-bound antibodies used in the cocktail.
	www.nationmaster.com /encyclopedia/Flow-immunophenotyping (148 words)

	Hematopathology: Immunophenotyping Lymphomas (Site not responding. Last check: 2007-11-06)
		The technique is called "immunophenotyping" for 2 reasons: 1) It is dependent on the activity of antibodies, which are immunological substances 2) It is used chiefly to identify lymphoid and hematopoietic cells, which are part of the immune system.
		Immunophenotyping is used clinically chiefly to diagnose and categorize lymphomas and leukemias.
		Immunophenotyping is helpful because in many instances different kinds of benign and malignant lymphoid cells resemble each other in routinely stained tissue sections and smears.
	pleiad.umdnj.edu /hemepath/immuno/immuno.html (1069 words)

	Consensus Leukemia Immunophenotyping
		The scope of flow cytometric immunophenotyping is to provide an objective and reproducible method for the diagnosis and therapy control of hematological malignancies, based on the immunological identification and characterization of clonally expanded abnormal precursor cells of the various hematopoietic lineages.
		Immunophenotyping also contributes to the further characterization of AML subtypes, especially the M0 and M7 subtype, while the FAB-definition of M1 to M6 is still based mainly on morphology and cytochemistry.
		The major goals of immunophenotyping in mature lymphoid neoplasias currently are, therefore, the assignment of abnormal cells to the B or T cell lineage, their maturational analysis, and the characterization of specific phenotypes which might be helpful for the subclassification of disease.
	www.biochem.mpg.de /valet/eurocel2.html (11306 words)

	__________________________ (Site not responding. Last check: 2007-11-06)
		Flow cytometry is usually used for routine immunophenotyping because of its automated nature.
		Immunophenotyping was performed in 20 of the cases with both flow cytometry and APAAP method and only the results of these were compared.
		Immunophenotyping was needed for the diagnosis of the remaining three cases.
	www.istanbul.edu.tr /istanbultip/mecmua/medmecmua/med-99-1/03.htm (1536 words)

	ASCO - General Information - Onsite Details (Site not responding. Last check: 2007-11-06)
		Flow cytometry measures physical and/or chemical characteristics of individual cells as the cells pass singly, in a fluid stream, by one or more optical or electronic sensors.
		The clinical utility of flow cytometry is in establishing the immunophenotype of hematolymphoid neoplasms and in evaluating the ploidy and S-phase fraction of solid tumors.
		Immunophenotyping is denied as not medically necessary in patient with lymphocytosis for purposes of establishing a diagnosis of chronic lymphocytic leukemia.
	www.asco.org /ac/1,1003,_12-002179-00_18-0018759,00.asp (1547 words)

	An Atlas with Flow Cytometry Utilized in AML Today
		Flow cytometric techniques are now routinely utilized in clinical hematology.
		Multiparameter flow cytometry measurements allow for the differential analysis of normal and abnormal cells within a peripheral blood or bone-marrow specimen.
		The major advantage offered by flow cytometry is that subsets of cells within a normal peripheral blood or bone marrow hematopoietic cell population can be identified by staining with antibodies that are conjugated with fluorescent dyes (fluorochromes) and recognize membrane and intracellular proteins (antigens).
	www.meds.com /leukemia/flow/flow0.html (541 words)

	Guidelines for the Performance of CD4+ T-Cell Determinations in Persons with Human Immunodeficiency Virus Infection
		Immunophenotyping relies on detecting specific antigenic determinants on the surface of WBCs by antigen-specific monoclonal antibodies that have been labeled with a fluorescent dye or fluorochrome, such as phycoerythrin (PE) or fluorescein isothiocyanate (FITC).
		Flow cytometric immunophenotyping is a highly complex and relatively new technology.
		One method is to flush the flow cytometer fluidics with a 10% bleach solution for 5-10 minutes at the end of the day, then flush with water or saline for at least 10 minutes to remove excess bleach, which is corrosive.
	www.cdc.gov /mmwr/preview/mmwrhtml/00019952.htm (5439 words)

	About Flow Cytometry (Site not responding. Last check: 2007-11-06)
		Basically, a "generic" flow cytometer is similar to a hematology cell counter, using a 488 nm argon ion laser as an excitation light source and being equipped with additional detectors for fluorescence signal measurement.
		Flow cytometric procedures start with procurement of cells or tissues, proceed through staining and washing, continue through acquisition of flow data on the cytometer, and culminate in data analysis and the reporting of results.
		Flow cytometric analysis of cellular DNA content in the clinical laboratory has been used to characterize solid tumors, in addition to lymphoreticular and hematopoietic neoplasms ( 9).
	flowsite.hitchcock.org /AboutFlow/main.html (4017 words)

	Applied Technology Products, Inc (Site not responding. Last check: 2007-11-06)
		Immunophenotyping depends upon the ability to remove the neoplastic cells from the body, and the presence or absence of neoplastic cell antigens is determined by a direct analysis of the cells themselves.
		Flow cytometric analysis of antibody conjugates will be required before and after their addition to the blood samples.
		The new procedure proposed here is based upon a flow cytometric analysis of antibodies in a panel that do not become bound to the cells in the sample and may be called reverse immunophenotyping.
	www.appliedtechnologyproducts.com /IMAGRAD1995.htm (2163 words)

	The Body: 1997 Revised Guidelines forPerforming CD4+ T-Cell Determinationsin Persons Infected withHuman ...
		Immunophenotyping refers to the detection of antigenic determinants (which are unique to particular cell types) on the surface of WBCs using antigen-specific monoclonal antibodies that have been labeled with a fluorescent dye or fluorochrome (e.g., phycoerythrin [PE] or fluorescein isothiocyanate [FITC]).
		The fluorochrome-labeled cells are analyzed by using a flow cytometer, which categorizes individual cells according to size, granularity, fluorochrome, and intensity of fluorescence.
		Flow cytometric analyses of T-cell subsets using three- and four-color approaches (in contrast to the two-color approach addressed in previous reports [35,36]), flow cytometric analyses for measuring both the proportion and the absolute numbers of CD4+ T-lymphocytes, and other methods for deriving an absolute CD4+ T-cell count in a blood sample are now commercially available.
	www.thebody.com /cdc/cd4/cd4-1.html (861 words)

	MEDIC - CME Online - Flow Cytometry (Site not responding. Last check: 2007-11-06)
		Flow cytometry is the quantitative multiparameter measurement of chemical or physical characteristics of cells in suspension.
		Immunophenotyping is the most useful in differentiating AML from ALL and has not been particularly useful in subclassifying AML or for prognostic purposes.
		Therefore, immunophenotypic evidence of T-cell malignancies rests on abnormal/aberrant antigen expression and or the presence of a DNA abnormality.
	medic.med.uth.tmc.edu /edprog/00000014.htm (3180 words)

	IMMUNOPHENOTYPING OF ACUTE LEUKEMIA AT KING FAISAL SPECIALIST HOSPITAL AND RESEARCH CENTRE:15(2)
		We conclude that immunophenotyping by flow cytometry is a useful and reliable method for classification of acute leukemia, especially when interpreted in the light of morphology and cytochemistry.
		Eleven bone marrows submitted for immunophenotyping with the clinical diagnosis of chronic lymphocytic leukemia and bone marrow involvement by lymphoma, as well as 13 bone marrows submitted showing no clonal expansion, with the diagnosis of chronic granulocytic leukemia in questionable transformation which turned out to be in chronic phase morphologically, were excluded from the study.
		Flow cytometry in the diagnosis of acute leukemia.
	www.kfshrc.edu.sa /annals/152/94109ar.html (1599 words)

	Amazon.com: Books: Flow Cytometry Principles for Clinical Laboratory Practice: Quality Assurance for Quantitative ... (Site not responding. Last check: 2007-11-06)
		Flow Cytometry Principles for Clinical Laboratory Practice is an invaluable benchtop reference and introduction for first-time users of flow cytometry in clinical diagnostic laboratories.
		CD4+ lymphocyte determination by immunophenotyping is the standard method for evaluating the status of HIV infection, and the single most frequent application of flow cytometry in a diagnostic setting.
		Flow cytometry is a powerful technique for obtaining multiparameter data on a wide variety of particles or cells.
	www.amazon.com /exec/obidos/tg/detail/-/0471021768?v=glance (613 words)

	British Journal of Biomedical Science: Immunophenotyping
		Flow cytometry is one of the most operator-dependent semi-automated procedures used in the clinical laboratory.
		Immunophenotyping in HIV infection is described in Chapter 11, which is particularly well presented and includes a succinct synopsis of the evolution of knowledge about HIV.
		The final chapter on combined immunophenotyping and molecular phenotyping discusses the potential of what, for most laboratories, may be a future project, and provides an incentive to overcome the problems with implementation, provision of suitable reagents and reproducibility.
	www.findarticles.com /p/articles/mi_qa3874/is_200101/ai_n8939637 (624 words)

	Flow Cytometry
		The technology of flow cytometry and the discovery of a method to produce monoclonal antibodies have made possible the clinical use of flow cytometry for the identification of cell populations.
		The flow cytometer is able to rapidly screen large numbers of cells far beyond the capacity of traditional pathological or cytological methods.
		Subpopulations of lymphocytes are identified and quantitated by the flow cytometer by utilizing monoclonal antibodies to various cell surface antigens.
	www.pathlabsofark.com /flowcytometry.html (353 words)

	1997 Revised Guidelines for Performing CD4+ T-Cell Determinations in Persons Infected with Human Immunodeficiency Virus ...
		Note: Software on the flow cytometer must be capable of using the information obtained from these monoclonal antibody combinations to correctly identify lymphocytes and to extrapolate that information to determine the percentage of lymphocytes that are CD4+ and CD8+ T-cells (63).
		Analyze this material and adjust the electronic compensation circuits on the flow cytometer to place the fluorescent populations in their respective fluorescence quadrants with no overlap into the double-positive quadrant ( Figure_1).
		Use of flow cytometry to enumerate lymphocyte populations in HIV disease.
	www.cdc.gov /mmwr/preview/mmwrhtml/00045580.htm (8676 words)

	The Body: 1997 Revised Guidelines forPerforming CD4+ T-Cell Determinationsin Persons Infected withHuman ...
		Use one tube containing K3EDTA when a) hematology and flow cytometric immunophenotyping will be performed in the same laboratory on the same specimen or b) a single measurement is performed on the flow cytometer that results in an absolute number.
		The effect of cool temperatures (i.e., 39 F [4 C]) on immunophenotyping results is not clear ( 52, 57).
		Analyze this material and adjust the electronic compensation circuits on the flow cytometer to place the fluorescent populations in their respective fluorescence quadrants with no overlap into the double-positive quadrant (Figure 1).
	www.thebody.com /cdc/cd4/cd4-2.html (5138 words)

	Diagnostic utility of flow cytometric immunophenotyping in myelodysplastic syndrome -- Stetler-Stevenson et al. 98 (4): ...
		Immunophenotypic myeloid abnormalities were considered to be present only when 2 or more abnormalities were detected.
		by flow cytometry and clonal karyotypic abnormalities by
		Flow cytometric immunophenotypic abnormalities in a single lineage were detected in bone marrow aspirates from 4 of 15 aplastic
	www.bloodjournal.org /cgi/content/full/98/4/979 (5079 words)

	Acute Leukemia Profile, Flow Cytometry (Site not responding. Last check: 2007-11-06)
		Multiparameter analysis using four-color immunophenotyping techniques is a rapid and specific method of assigning lineage in acute leukemia.
		When the morphology and immunophenotype suggest a diagnosis of acute promyelocytic leukemia (APL), confirmation by testing for the retinoic acid receptor gene rearrangement by cytogenetic or molecular methods is recommended.
		Immunophenotyping and cytogenetic analysis are increasingly being used to supplement the traditional methods (morphology and cytochemistry) of classifying acute leukemias and to provide prognostic information.
	www.labcorp.com /datasets/labcorp/html/chapter/mono/dp000100.htm (493 words)

	Flow cytometric immunophenotyping of serous effusions and peritoneal washings: comparison with immunocytochemistry and ...
		The role of conventional cytology, immunocytochemistry, and flow cytometric DNA ploidy in evaluation of body cavity fluids: a prospective study of 52 patients.
		Multiparameter flow cytometric DNA analysis of effusions: a prospective study of 36 cases compared with routine cytology and immunohistochemistry.
		Flow cytometric identification of cancer cells in effusions with Ca1 monoclonal antibody.
	jcp.bmjjournals.com /cgi/content/full/53/7/513 (2776 words)

	AFCG Lymphocyte Immunophenotyping Standard (Site not responding. Last check: 2007-11-06)
		The aim of lymphocyte immunophenotyping is to enumerate and identify specific sets or subsets of lymphocytes.
		Specimens with pronounced leucopaenia may have insufficient cells for flow cytometric analysis, thus requiring a larger volume of sample or a buffy coat preparation.
		Assessment of specimen viability is desirable however because of biohazard concerns, it is recommended that all samples be appropriately fixed prior to analysis on the flow cytometer.
	jcsmr.anu.edu.au /facslab/AFCG/Standards/lymphimm.html (2257 words)

	Clinically Useful Information Provided by the Flow Cytometric Immunophenotyping of Hematological Malignancies: Current ...
		Immunophenotyping in the diagnosis of chronic lymphoproliferative disorders.
		Immunophenotyping investigation of minimal residual disease is a useful approach for predicting relapse in acute myeloid leukemia patients.
		Immunophenotyping of acute leukemia by flow cytometric analysis: use of CD45 and right-angle light scatter to gate on leukemic blasts in three-color analysis.
	www.clinchem.org /cgi/content/full/45/10/1708 (7430 words)

	BIOMED-1 concerted action report: flow cytometric immunophenotyping of precursor B-ALL with standardized ...
		The next question to be adressed concerns the sensitivity levels of the flow cytometry immunophenotypic approach for the identification of MRD in precursor-B-ALL described herein and the lowest number of events to be acquired for the accurate measurement of the residual leukemic cells.
		Significance of aberrant immunophenotypes in childhood acute lymphoid leukemia.
		Flow cytometric analysis of normal B cell differentiation: a frame of reference for the detection of minimal residual disease in precursor-B-ALL.
	www.nature.com /leu/journal/v15/n8/full/2402150a.html (5551 words)

	Clinical Flow Cytometry Case #53
		Because of his cervical lymphadenopathy, splenomegaly and the atypical lymphocytes in the periphery, a flow cytometric immunophenotyping study and a subsequent molecular study were performed on the peripheral blood.
		Becasue of the patient's clinical presentation, atypical lymphocytosis, a negative monospot test and the flow cytometric immunophenotyping results, a polymerase chain reaction assay for the T-cell receptor gamma gene rearrangement was performed.
		A repeat flow cytometric immunophenotyping on the peripheral blood three weeks later showed complete disappearance of the CD3+CD8dim+ T cell population and the patient's clinical signs and symptoms of acute infectious mononucleosis resolved.
	www.flowcases.org /casebuilder/index.cfm?fuseaction=displayCase2&caseID=53 (625 words)

	LAH Publications 400-499 (Site not responding. Last check: 2007-11-06)
		However, the multiparameter flow cytometry methodology used here reveals changes in a substantially larger number of surface markers, some of which are restricted to fine subsets of PBMC, such as naive or memory T cell subsets.
		However, multicolor flow cytometry comes with a new and sometimes difficult set of technical problems that must be overcome by users to derive meaningful results.
		The histories of monoclonal antibodies and FACS (flow cytometry instruments) are as closely intertwined as their uses today in biology and medicine.
	herzenberg.stanford.edu /Publications/400-499.htm (12996 words)

Try your search on: Qwika (all wikis)