| | ARS | Publication request: The Fluidity of Chinese Hamster Ovary Cell and Bull Sperm Membranes after Cholesterol ... |
 | | CHO cell membrane fluidity did not change when temperature was reduced, but adding cholesterol to the cells induced membranes to become more rigid. |
 | | In conclusion, cholesterol treatment resulted in detectable membrane fluidity changes in CHO cells and increased survival of bull sperm after cooling to 5°C and after cryopreservation. |
 | | Cells were treated with 0, 1.5 or 5.0mg of cholesterol-loaded cyclodextrin (CLC), stained with N-((4-(6-phenyl-1,3,5-hexatrienyl) phenyl) propyl) trimethylammonium p-toluenesulfonate (TMAP-DPH) to evaluate membrane fluidity and with propidium iodide to evaluate cell viability, prior to analysis by flow cytometry at 23°C, 5°C and after cryopreservation. |
| www.ars.usda.gov /research/publications/Publications.htm?seq_no_115=182868 (486 words) |