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Topic: Fluorescein

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Fluorescein angiography

Fluorescein angiogramic angiography

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	Fluorescein - Wikipedia, the free encyclopedia
		Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, and in forensics and serology to detect latent blood stains.
		Fluorescein angiography is used to diagnose and categorize age-related macular degeneration, diabetic retinopathy, inflammatory intraocular conditions, and intraocular tumors.
		Absorption and Emission Spectra of Fluorescein in Ethanol
	en.wikipedia.org /wiki/Fluorescein (466 words)

	Fluorescein angiography - Wikipedia, the free encyclopedia
		Fluorescein angiography, or fluorescent angiography, is a technique for examining the circulation of the retina.
		It involves injection of sodium fluorescein into the systemic circulation, and then an angiogram is obtained by photographing the fluorescence emitted after illumination of the retina with blue light at a wavelength of 490 nanometers.
		Fluorescein enters the ocular circulation from the internal carotid artery via the ophthalmic artery.
	en.wikipedia.org /wiki/Fluorescein_angiography (428 words)

	Dark Reader - Fluorescein
		Fluorescein (ex = 492 nm, em = 525 nm) is one of the most widely used fluorophors and is employed as a detection agent in numerous assays.
		The Dark Reader is at least twice as sensitive as a UV device for the detection of fluorescein and up to 16-fold when the dye is in a test-tube or in a gel apparatus.
		A simplified summary of the basic protocols for the detection of biotinylated proteins by either (A) fluorescein fluorescence or (B) luminol luminescence is shown in the Figure.
	www.clarechemical.com /fluorescein.htm (321 words)

	FITC conjugation of Antibodies
		Fluorescein (mistakenly abbreviated by its commonly-used reactive isothiocyanate form, FITC) is currently the most commonly-used fluorescent dye for FACS analysis.
		Fluorescein is typically excited by the 488 nm line of an argon laser, and emission is collected at 530 nm.
		The reactive fluorescein molecule, fluorescein isothiocyanate, is unstable.
	www.drmr.com /abcon/FITC.html (769 words)

	Fluorescein Angiogram
		Fluorescein angiography is a test which allows the blood vessels in the back of the eye to be photographed as a fluorescent dye is injected into the bloodstream via your hand or arm.
		Although commonly referred to as fluorescein, the dye used in angiography is fluorescein sodium, the sodium salt of fluorescein.
		Fluorescein angiography is most frequently used for evaluation of patients with diabetic retinopathy, occlusive diseases such as retinal vein and arterial occlusions, and evaluation for wet macular degeneration.
	www.mdsupport.org /library/angio.html (1020 words)

	Trace Organic Analysis - Detection of Fluorescein, EPA
		The concentration of fluorescein was calculable based on the known volume and concentration of internal standard added to the sample and the known volume/weight of the sample being analyzed.
		Results for fluorescein are reported as ppt-levels in the 10-mL extractant of the pads or ppt in the water when determined directly from a portion of the water sample.
		Fluorescein was found to have an absorption maximum near 488 nm in DI water.
	www.epa.gov /esd/chemistry/org-anal/reports/lif488/lif488.htm (3284 words)

	PetPlace.com - Article: Fluorescein Stain (Site not responding. Last check: 2007-09-06)
		Fluorescein is an orange stain that is applied to the cornea to reveal corneal lesions.
		Fluorescein staining is also used to determine if the duct that allows passage of tears from the corner of the eye to the nose is open and working.
		Fluorescein staining of the cornea identifies abrasions, scratches, ulcerations and lacerations present in the cornea on the surface of the eye.
	www.petplace.com /netscape/nsArtShow.asp?artID=4125 (535 words)

	Fluorescein and indocyanine green angiography (Site not responding. Last check: 2007-09-06)
		Fluorescein and indocyanine green angiography are diagnostic tests which use special cameras to photograph the structures in the back of the eye.
		Fluorescein is a yellow dye which glows in visible light.
		Fluorescein angiography is universally employed throughout the world as a diagnostic test.
	www.vrmny.com /angiography.htm (720 words)

	Fluorescein Angiography
		Fluorescein Angiography is used in the diagnosis and treatment of a wide range of ocular disorders.
		Medical necessity of Fluorescein Angiography is established as an adjunct to the diagnosis of choroiretinal vascular abnormalities especially relating to choroid neovascularization, non infective vasculitis, age related macular degeneration.
		Fluorescein Angiography is appropriate as a baseline study for patients who are projected to be on long term hydroxychloroquine sulfate (Plaquenil).
	www.cignamedicare.com /partb/lmrp/id/cms_fu/97-014.htm (710 words)

	Fluorescein As A Field Worthy Latent Bloodstain Detection System
		Fluorescein was considered to be the reagent with the greatest potential in the detection of latent bloodstains in the California Criminalistics Institute (CCI) Maucieri and Monk study in 1991.
		By attaining the fore mentioned goals, it is hoped that the fluorescein technique will be appreciated as a truly field worthy and practical procedure remaining within the scope and skill levels of crime scene technicians and investigators, hence resulting in an improvement in documentation.
		In previous literature, fluorescein (oxidized color state) was reduced to fluorescin (reduced colorless state) via heating the fluorescein (1.0 gram) in 10% NaOH (100ml) with 10.0 grams zinc powder.
	www.arcanaforensics.com /fluorescein.html (3222 words)

	Fluorescein Angiography: definition, about the test, potential risks - Kellogg Eye Center
		Fluorescein angiography is a diagnostic procedure which uses a special camera to take a series of photographs of the retina, or light sensitive tissue in the back of the eye.
		Fluorescein angiography is often done to follow the course of a disease such as diabetes, a disease that can cause the blood vessels of the retina to leak blood or fluid.
		Without the help of fluorescein angiography, your ophthalmologist would not know exactly where a leak is to guide the pinpoint accuracy of laser treatment.
	www.kellogg.umich.edu /patientcare/conditions/fluorescein.angiography.html (469 words)

	HGSA Medical Policy: M-36B - Fluorescein Angiography (Site not responding. Last check: 2007-09-06)
		Following the intravascular administration of a contrast solution of sodium fluorescein, a blue light is used to excite the fluorescein which is useful in detecting leaking capillaries (subretinal neovascularization).
		The frequency of the fluorescein angiography is dependent on the extent of the disease progression and the treatment performed (i.e., photocoagulation).
		Fluorescein angiography may be performed as often as every 8-12 weeks to assist in management of the retinopathy.
	www.hgsa.com /professionals/policy/m36b.html (1140 words)

	SAFC Supply Solutions - Proligo® Reagents - Fluorescein Phosphoramidite
		Fluorescein is used as a non-radioactive label with a high extinction coefficient and an excellent fluorescence quantum yield.
		Fluorescein, which can be excited by argon-ion lasers at 488nm, combines good water solubility with a low aggregation tendency.
		Protecting groups on the fluorescein moiety are removed under standard conditions of cleavage and deprotection with concentrated ammonia.
	www.sigmaaldrich.com /SAFC/Supply_Solutions_Proligo/bOur_Offerb/Fluorescein_Phosphoramidite.html (200 words)

	Fluorescein Angiography (Site not responding. Last check: 2007-09-06)
		Fluorescein angiography, a clinical test to look at blood circulation inside the back of the eye, aids in the diagnosis of retinal conditions associated with diabetes, age-related macular degeneration, and other eye abnormalities.
		Fluorescein, a harmless orange-red dye, is injected into a vein in the arm.
		There is little risk in having fluorescein angiography, though some people may have mild allergic reactions to the dye.
	www.bouldereyesurgeons.com /retina7.htm (264 words)

	Frequently Asked Questions - New York Eye & Ear Infirmary
		Fluorescein angiography is photographic record of fluorescein dye (food coloring) passing through the blood vessels of the film or retina of the eye.
		The dye fluorescein is water soluble and has the property to fluoresce, that is to emit a specific color of light when stimulated.
		The fluorescein dye in the blood vessels allows them to be easily seen in the photographs, so the vessels can be identified and evaluated.
	www.nyee.edu /faqlist.html?tablename=faq&key=20 (794 words)

	Fluorescein Angiography - WSAVA 2002
		Fluorescein angiography is a dynamic method to explore the fundus vasculature and tissues.
		The fluorescent dye used is sodium fluorescein injected intra venously.
		The intravenous injection of fluorescein is done just after having changed the color camera by the fl and white one.
	www.vin.com /proceedings/Proceedings.plx?CID=WSAVA2002&PID=2650 (1060 words)

	Fluorescein Angiography - FA (Site not responding. Last check: 2007-09-06)
		Fluorescein angiography (fluorescein - the type of dye that is used; angiogram - a study of the blood vessels) is an extremely valuable test that provides information about the circulatory system of the back of the eye not attainable with a routine examination.
		The test is performed by injecting a special dye called fluorescein into a vein in the arm.
		A camera equipped with special filters that highlight the dye is used to photograph the path of the fluorescein as it circulates though the eye.
	www.theretinasource.com /tests/fluorescein_angiography.htm (158 words)

	OPS - Fluorescein Angiography (Site not responding. Last check: 2007-09-06)
		Fluorescein sodium is a highly fluorescent chemical compound synthesized from the petroleum derivatives resorcinol and phthalic anhydride.
		These fluorescent properties have made fluorescein useful in a variety of industrial, scientific and medical applications since the dye was first synthesized by Von Baeyer in 1871.
		Fluorescein is well tolerated by most patients, but angiography is an invasive procedure with an associated risk of complication or adverse reaction.
	www.opsweb.org /Op-Photo/Angio/FA/FA2.htm (490 words)

	Invitrogen - Molecular Probes - Section 1.5 - Fluorescein, Oregon Green and Rhodamine Green Dyes
		Furthermore, fluorescein's relatively high photobleaching rate limits the sensitivity that can be obtained, a significant disadvantage for applications requiring ultrasensitive detection, such as DNA sequencing (Section 8.2), fluorescence in situ hybridization (Section 8.5) and localization of low-abundance receptors.
		Fluorescein is frequently used as a hapten on a primary detection reagent that can be either amplified or converted into a longer-wavelength or electron-dense signal with the appropriate secondary detection reagent.
		Because of the near match of their absorption maxima on proteins (~498 nm and ~512 nm) to the strong 488 nm and 514 nm spectral lines of the argon-ion laser, the Oregon Green 488 and Oregon Green 514 fluorophores are important dyes for both confocal laser-scanning microscopy and flow cytometry applications.
	probes.invitrogen.com /handbook/sections/0105.html (3719 words)

	:: Fluorescein Angiography :: (Site not responding. Last check: 2007-09-06)
		Damage to the lining un-derneath the retina or the appearance of abnormal new blood vessels growing beneath the retina may also be revealed.
		The precise location of these abnormali-ties may be determined by a careful interpretation of the fluorescein angiogram by your eye doctor.
		Without the help of fluorescein angiog-raphy, your eye doctor would not be able to thoroughly diagnose these and other abnormalities.
	www.specialisteyecentre.com /eye/fluorescein.html (396 words)

	Ophthalmic Diagnostic Photography UIHC Ophthalmology and Visual Sciences
		A contrast medium called Sodium Fluorescein is injected into a vein in the arm.
		A fluorescein angiogram of a patient with ocular complications due to diabetes (diabetic retinopathy) reveals vascular irregularities when compared with the normal angiogram.
		Fluorescein angiography is a test which helps in the differentiation of retinal disease and is used to determine if laser treatment of the retina is warranted.
	webeye.ophth.uiowa.edu /dept/SERVICE/PHOTO/plfluor.htm (229 words)

	Surface Uptake and Intercalation of Fluorescein Anions into Zn-Al-Hydrotalcite. Photophysical Characterization of ...
		The xanthene dye fluorescein was immobilized, via anion-exchange reactions, in the interlayer region and/or on the surface of a synthetic Zn-Al hydrotalcite, prepared by the thermal hydrolysis of urea.
		Intercalation of fluoresceinate anions occurs with a first-order phase transition from the ClO
		Computer models based on the structure of the host, the composition and interlayer distance of the intercalation compound, and the van der Waals dimensions of the guest showed that fluorescein anions are incorporated in the interlayer region as a bilayer of species, with the main axis parallel to the layer plane.
	pubs.acs.org /cgi-bin/abstract.cgi/langd5/2000/16/i26/abs/la001096d.html (380 words)

	Fluorescein isothiocyanate (FITC) Antibody and Reagent Products Catalog Number 11
		11-0498 Fluorescein isothiocyanate (FITC) anti-human CD49b (Integrin alpha 2) - eBioY418
		11-5711 Fluorescein isothiocyanate (FITC) anti-mouse gamma delta TCR (gd-TCR, gdTCR) - GL3 (GL-3)
		11-7581 Fluorescein isothiocyanate (FITC) anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker) - BZ332
	www.ebioscience.com /ebioscience/sitemap/antibody_11.htm (1858 words)

	The Saccharomyces cerevisiae Weak-Acid-Inducible ABC Transporter Pdr12 Transports Fluorescein and Preservative Anions ...
		The Saccharomyces cerevisiae Weak-Acid-Inducible ABC Transporter Pdr12 Transports Fluorescein and Preservative Anions from the Cytosol by an Energy-Dependent Mechanism -- Holyoak et al.
		The inhibition of glucose-induced Pdr12-catalyzed extrusion of fluorescein by increasing concentrations (0.9 and 1.8 mM) of
		Rates were calculated from the slope of the linear region of plots showing glucose-induced fluorescein efflux in the presence of increasing concentrations of preservatives.
	jb.asm.org /cgi/content/full/181/15/4644 (5786 words)

	Fluorescein antibody hrp conjugate (ab6656) datasheet (Site not responding. Last check: 2007-09-06)
		If Fluorescein antibody (ab6656) does not perform as described on this datasheet, notify us within 90 days of delivery using this form, so that we can help you or offer you a replacement or a refund.
		Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation.
		Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm.
	www.abcam.com /index.html?datasheet=6656&CFID=5311104&CFTOKEN=75220004 (387 words)

	The presumptive reagent fluorescein for detection of dilute bloodstains and subsequent STR typing of recovered DNA. ... (Site not responding. Last check: 2007-09-06)
		The presumptive reagent fluorescein for detection of dilute bloodstains and subsequent STR typing of recovered DNA.
		However, background staining for fluorescein was observed on some substrates within 30 s to 1 min, and no background staining was observed for luminol.
		The sensitivity of detection of fluorescein was comparable to that of luminol in this study.
	www.astm.org /jforensicsci/PAGES/3381.htm (248 words)

	MedlinePlus Medical Encyclopedia: Fluorescein eye stain
		This is a test that uses orange dye (fluorescein) and a blue light to detect foreign bodies in the eye.
		A piece of blotting paper containing fluorescein dye will be touched to the surface of your eye.
		If the fluorescein touches the skin surface, there may be a slight, brief, discoloration.
	www.nlm.nih.gov /medlineplus/ency/article/003845.htm (591 words)

	Nikon MicroscopyU: Fluorescence Microscopy - Filter Combinations (YFP HYQ) - HeLa Cell Histones with Fluorescein
		The visible light absorption maximum of fluorescein is 490 nanometers and the emission maximum occurs at 525 nanometers, values that fall within the useful range of the Nikon YFP HYQ filter combination.
		The primary antibody, produced from purified nuclear fractions of HeLa cells, binds specifically to an antigen determinant that is present on all five histone proteins (H1, H2A, H2B, H3, and H4).
		Note the strong green fluorescence emission exhibited by both the interphase nuclei and those in various stages of mitosis, while no signal is observed from the blue and red fluorophores (Alexa Fluor 350 and MitoTracker Red) targeting actin and mitochondria, respectively.
	www.microscopyu.com /articles/fluorescence/filtercubes/yfp/yfphyq/stains/yfpfitchistonehelacells.html (187 words)

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