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Topic: GFP

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	GFP BUNNY
		Alba is a participant in the "GFP Bunny" transgenic artwork; so is anyone who comes in contact with her, and anyone who gives any consideration to the project.
		"GFP Bunny" highlights the fact that transgenic animals are regular creatures that are as much part of social life as any other life form, and thus are deserving of as much love and care as any other animal [16].
		"GFP Bunny" does not propose any new form of genetic experimentation, which is the same as saying: the technologies of microinjection and green fluorescent protein are established well-known tools in the field of molecular biology.
	www.ekac.org /gfpbunny.html (8829 words)

	GFP Symposium backround information
		GFP applications abound in such areas as cell and developmental biology, neurobiology, and cytology where the time and location of specific gene activation can be determined by fluorescence microscopy, confocal microscopy, and image intensification.
		GFP is also being developed for use in high throughput cell-based diagnostic tests for drugs, food additives, herbicides, pesticides, carcinogens, mutagens, teratogens, and other potentially hazardous chemicals.
		The brilliant fluorescence of GFP and the ability to manipulate and express the GFP gene in foreign cells and organisms make this protein an ideal tool for laboratory instruction in biotechnology at all educational levels from grade school through postgraduate.
	www.rci.rutgers.edu /~meton/SymDetails.html (709 words)

	Green Fluorescent Protein - GFP
		Now GFP is found in laboratories all over the world where it is used in every conceivable plant and animal.
		This is done by joining the GFP gene to the gene of the protein of interest so that when the protein is made it will have GFP hanging off it (see Figure below).
		Since GFP fluoresces one can shine light at the cell and wait for the distinctive green fluorescence associated with GFP to appear.
	www.conncoll.edu /ccacad/zimmer/GFP-ww/GFP-1.htm (215 words)

	GFP Fusions
		This is a technique in which the GFP fluorescence in a defined region of the dendrite is 'bleached' by repeated exposure to high intensity laser light.
		GFP was fused to the C-terminus of the mGlu1
		GFP itself is expressed in all cellular compartments.
	www.bris.ac.uk /synaptic/research/projects/GFP.html (780 words)

	GFP tags for the Synapse
		GFP is a very stable molecule and the chromophore is well protected and in a very insulated local environment in the middle of the cylinder.
		Nevertheless, both N- and C- terminal fusions are fluorescent consistent with the N- and C-terminus of GFP being flexible enough not to disrupt the general structure of the molecule.
		In this case, GFP is added the N- instead of C-terminus because RAB-3 is post-translationally modified with the addition of a geranylgeranyl hydrophobic moiety to C-terminal cysteines which act as its anchor to the synaptic vesicle membrane.
	thalamus.wustl.edu /nonetlab/ResearchF/GFPtags.html (525 words)

	GFP (Site not responding. Last check: 2007-10-26)
		GFP is a 27 kDa protein from the pacific jellyfish Aequorea victoria.
		GFP is normally in a complex with aequorin, a protein that emits blue light when it binds to calcium.
		GFP has the remarkable property of encoding its fluorophore in its primary structure, so it becomes fluorescent when translated in any cell type.
	www.gurdon.cam.ac.uk /~pineslab/Projects/gfp.htm (273 words)

	GFP Structure (Site not responding. Last check: 2007-10-26)
		GFP is very resistant to denaturation, requiring treatment with 6 M guanidinium hydrochloride at 90° centigrade or a pH smaller than four or greater than twelve [6].
		This is due to the fact that both termini of GFP appear rather flexible on the surface of the beta-can, so that GFP's structure is not significantly distorted or destroyed by the fused protein.
		GFP has a single tryptophan, Trp 57 (blue), which is located 13 to 15 Å away from the chromophore (red) [7].
	www.cryst.bbk.ac.uk /PPS2/projects/jonda/structur.htm (920 words)

	GFP\| Guaranteed Funeral Deposits of Canada
		GFP Guaranteed Funeral Plan is a group insurance product offered by a select group of Funeral Homes that are members of Guaranteed Funeral Deposits of Canada Fraternal.
		With GFP Guaranteed Funeral Plan, when you pay for your funeral in a lump sum, you qualify for a discount based on your age at the time of purchase.
		When you purchase a GFP plan, you are eligible to enroll in the World Wide Travel Plan™.
	www.gfd.org /Visitors/Funding/gfp.aspx (240 words)

	Cytosolic expression of GFP and its derivatives in the yeast Saccharomyces cerevisiae:
		GFP is a 238 amino acid protein isolated from the fluorescent jellyfish, Aequoria victoria.
		GFP is an extremely attractive tool for today's life scientist, mainly because the protein matures without the need for any additional substrates or co-factors.
		GFP has allowed the real time study of cellular events in live, intact cells and organisms, allowing a better understanding of biological mechanisms in physiological models.
	www.varianinc.com /nav/applications/apps/app07&cid=IPMPMJOMFQ (982 words)

	TechOnline \| Transparent Generic Framing Procedure (GFP)
		GFP standardization began in the ANSI-accredited T1X1 subcommittee, which chose to work with the ITU-T for the final version of the standard that has been published by the ITU-T. The transparent version of GFP has been optimized for transparently carrying block-coded client signals with a minimum of latency.
		The payload area of the GFP frame is scrambled with a self-synchronous scrambler to address both the physical properties of the transport medium and the desire for robustness in public networks.
		Far-end client-specific performance reporting allows both ends of the GFP link to see the status of both directions of the GFP link, which is valuable when one of the ends is in an unmanned office or when the link crosses carrier domains.
	www.techonline.com /showArticle.jhtml?articleID=192200484 (3804 words)

	GFP Chromophore (Site not responding. Last check: 2007-10-26)
		GFP is unique among fluorescent proteins in that its fluorophore is not a seperately synthesized prostethic group but composed of modified amino acid residues within the polypeptide chain.
		Denatured GFP is not fluorescent and its absorbtion spectrum is significantly different from native GFP [1], which implies that noncovalent interactions of the chromophore with its local environment have a great influence on the spectral characteristics and that fluorescence is mediated by amino acids close to the chromophore in the tertiary structure of GFP.
		GFP is the first known example of a Förster cycle within the core of a protein [5,6,7]:
	www.cryst.bbk.ac.uk /PPS2/projects/jonda/chromoph.htm (1190 words)

	GFP as a Reporter
		GFP was the first of many flourescent proteins to be used as a reporter protein.
		GFP has become so popular that mutant alleles have been created that produce different colors of light.
		Highlight the chromophore portion of GFP which is composed of three modified amino acids.
	www.bio.davidson.edu /courses/genomics/method/GFP.html (346 words)

	CommsDesign - Handling GFP Implementation Tradeoffs in Metro Designs
		Multiple GFP engine architectures are possible: the GFP protocol may be contained wholly in a framer device, it may be contained wholly in the link-layer device, it may be completely integrated into a monolithic device, or it may be distributed between a link-layer device and a framer device.
		This mapping process forms a transparent L2 connection since the client data stream recreated at the egress side by the egress GFP engine is equivalent to the data stream presented to the ingress GFP engine with the exception of client protocol idle characters created or deleted for rate adaptation.
		The lowest-level GFP functions such as idle insertion and removal, receive synchronization and delineation, payload scrambling, and GFP frame FCS insertion/checking are present in the framer device.
	www.commsdesign.com /design_corner/showArticle.jhtml?articleID=17602987 (2834 words)

	AAMC: GFP : About GFP Membership
		A medical school may have a total of four representatives in the GFP; members are appointed by the dean of each LCME accredited medical school.
		In addition, GFP members receive the AAMC's weekly legislative and regulatory newsletter, Washington Highlights and the monthly membership letter, the Reporter.
		GFP members are able to submit their information to this database through a web-based interface.
	www.aamc.org /members/gfp/about.htm (703 words)

	GFP and Family: Illuminating High Throughput Drug Screening
		Interestingly, GFP and its family share a common protein structure and retain the auto-fluorescent chromophore.
		One unit of GFP fluorescence is equivalent to a single gene being expressed.
		The low cytotoxicity of GFP also lends it to be applied in live cell microscopy and to the monitoring of growth cultures in the fermentation industry.
	www.frost.com /prod/servlet/market-insight-top.pag?docid=60748148 (753 words)

	The Fluorophore of Green Fluorescent Protein (GFP)
		GFP is a fluorescent protein isolated from coelenterates, such as the Pacific jellyfish, Aequoria victoria, or from the sea pansy, Renilla reniformis.
		The gene for GFP has been isolated and has become a useful tool for making chimeric proteins of GFP linked to other proteins where it functions as a fluorescent protein tag.
		GFP tolerates N- and C-terminal fusion to a broad variety of proteins.
	dwb.unl.edu /Teacher/NSF/C08/C08Links/pps99.cryst.bbk.ac.uk/projects/gmocz/gfp.htm (1734 words)

	BioMed Central \| Full text \| Visualization of C. eleganstransgenic arrays by GFP
		GFP has also been targeted to specific subcellular structures by fusing GFP to various proteins.
		Based upon lacZ and GFP reporter gene constructs, lin-3 is expressed in the anchor cell at the time of vulval induction [14,21-23], and in the 1° vulval lineage after vulval induction [24].
		After a 30-minute heat-shock at 33°C, transformants were found to express nuclear GFP and have intense foci of subnuclear fluorescence, presumably corresponding to the DNA of the extrachromosomal arrays.
	www.biomedcentral.com /1471-2156/7/36 (4840 words)

	GE Healthcare Life Sciences - Green Fluorescent Protein (GFP)
		GFP Licenses: a broad portfolio of licenses to the complete range of "red-shifted" and "folding" mutations from Amersham Biosciences, Invitrogen IP Holdings Inc. and BioImage A/S, available from a single source.
		GFP is used in a very broad range of application areas and impacts on all parts of cell biology from the single cell to the whole animal
		GFP comprise a family of evolutionarily related proteins found in animals such as jellyfish and corals that belong to the phylum Cnidaria (stinging aquatic invertebrates).
	www4.amershambiosciences.com /APTRIX/upp00919.nsf/Content/drugscr_applications~drugscr_applic_technol~drugscr_gfp (854 words)

	The Molecular Structure of Green Fluorescent Protein
		The protein is in the shape of a cylinder, comprising 11 strands of -sheet with an -helix inside and short helical segments on the ends of the cylinder.
		GFP can function as a protein tag, as it tolerates N- and C-terminal fusion to a broad variety of proteins many of which have been shown to retain native function.
		The GFP from the sea pansy, Renilla reniformis, which exhibits a single major excitation peak at 498 nm, apparently utilizes an identical core fluorophore to that of A.
	www-bioc.rice.edu /Bioch/Phillips/Papers/gfpbio.html (4825 words)

	GFP
		GFP owes its fluorescence to the “autocatalytic cyclization of the polypeptide backbone between residues Ser
		GFP has a very particular barrel structure composed of 11 beta sheets, capped on one end by three helices and by one on the other.
		These are the characteristics of GFP which glows green when excited at certain wavelengths.
	www.soe.ucsc.edu /~gjjospin/GFP/GFP.htm (353 words)

	Generic Framing Procedure (GFP) Evaluation
		A Full System Hardware Evaluation version of the GFP core allows you to do everything you can do with the Fully Licensed IP core, including configure, place and route, simulate, estimate timing and program a Xilinx FPGA device.
		The GFP Core will appear in the right hand panel of the CORE Generator GUI.
		Please refer to Release Notes and Known Issues for GFP for the latest version of the Release notes for this core.
	www.xilinx.com /ipcenter/ipevaluation/gfp_evaluation.htm (532 words)

	What is GFP?
		This property makes GFP incredibly valuable for a wide range of applications in biomedical and genetic research, where it serves as a marker for genetic activity.
		The gene for the GFP can be transferred to bacteria, and colonies of bacteria can be used to grow supplies of the gene.
		There is a need for new GFP's in order to have better properties, such as new colors, brighter fluorescence, improved resistance to photobleaching, better compatibility for a wider range of experiments, and more, and some scientists are using NightSea equipment for exactly this purpose.
	www.nightsea.com /whatisgfp.htm (416 words)

	Circular permutant gfp insertion folding reporters patent invention
		[0006] In contrast to earlier described GFP folding reporter systems, in which a test protein is expressed as a C-terminal fusion with GFP in its native topology, the invention provides for test protein insertion within a circularly permuted fluorescent protein structure, specifically, between the native N- and C-termini of the fluorescent protein structure.
		C-terminal GFP folding reporters are unable to discriminate between artifact (i.e., a truncated protein) and a full length protein.
		For example, GFPcp9/8_FR/SF means that the GFP fragment in front of the guest insert protein is derived from folding reporter GFP, and the designation SF means the fragment of GFP after the guest insert protein is derived from superfolder GFP.
	www.freshpatents.com /Circular-permutant-gfp-insertion-folding-reporters-dt20061109ptan20060252063.php (4268 words)

	Generic Frame Protocol
		The Generic Frame Protocol (GFP), jointly developed at SRI International and Stanford University, provides a set of functions that support a generic interface to underlying frame representation systems (FRSs).
		GFP is based upon a generic model of frame representation systems (with frames, classes, slots, etc.) and consists of a set of access functions (e.g., get a frame by its name, change a slot's value in a frame).
		While GFP necessarily imposes some common requirements on the organization of knowledge (e.g., frames, slots, and values) and semantics of some assertions (e.g., instances and subclass relationship, and inherited slot values, slot constraints), it allows for some variety in the behavior of underlying FRSs.
	www.ai.sri.com /~gfp (389 words)

	BioMed Central \| Full text \| Imaging plant cell death: GFP-Nit1 aggregation marks an early step of wound and herbicide ...
		In the course of a screen for useful GFP fusion proteins [24], we observed that a GFP-Nitrilase 1 fusion protein exhibited a change of aggregation state in response to wounding.
		The N6 marker possesses a low background level of cytoplasmic GFP localization that might mask the release of nuclear contents and also may be partially immobilized from the bulk nucleoplasm by an association with DNA (this marker illuminates chromosomes during mitosis, [26]).
		Exclusion of cytosolic GFP from the interior of the lobes might be expected if this were true, since the space between the inner and outer nuclear envelopes, the peri-nuclear space, is contiguous with the lumen of the ER and thus isolated from the cytoplasm.
	www.biomedcentral.com /1471-2229/5/4 (6301 words)

	GFP Fusions (Site not responding. Last check: 2007-10-26)
		We are interested in the dynamics of glutamate receptor trafficking and have thus used GFP to directly visualise a number of these receptors.
		This is a technique in which the GFP fluorescence in a defined region of the dendrite is 'bleached' by repeated exposure to high intensity laser light.
		GFP was fused to the C-terminus of the mGlu1
	www.bristol.ac.uk /Depts/Synaptic/research/projects/GFP.html (780 words)

	davis lab > projects > genomic GFP fusions
		The advent of green fluorescent protein (GFP) as a means to tag and visualize proteins of interest in living cells has begun a revolution in cell and developmental biology.
		One of the most important aspects of the Wee-P GFP fusion proteins is that the expression is controlled by native regulatory sequences so the GFP-fusions can be expressed in the correct spatial and temporal patterns, as well as at the correct levels.
		Of the fusion proteins with essential genes that we have isolated to date, the vast majority are homozygous viable P-element insertions indicating that normal gene function is retained.
	www.ucsf.edu /davislab/projects/genomic_gfp.htm (418 words)

	InfinID Technologies : GFP for Learning Center
		GFP is a subset of PIPC (Property in Possession of Contractor) and consists of government property that has been made available to the contractor in order to perform a contract.
		When GFP items first arrive in the facility, an electronic submission of the GFP items must be sent to the UID Registry.
		Since all GFPs must be sent to the UID Registry, they all must have the basic information necessary for a UID, such as an enterprise identifier and serial number.
	www.infinidtech.com /?page=lc_gfp (361 words)

	DevSE-GFP
		At low magnifications GFP is difficult to detect, but as the magnification is increased, GFP becomes visible.
		The dried edge of the cotyledon in the bottom row might be mistaken for GFP under blue light, but when compared side by side with GFP expressing tissue under the microscope, a difference can be distinguished.
		For example, in the image on the right, a few faint spots of GFP are present, but the camera does not pick up the GFP fluorescence.
	www.oardc.ohio-state.edu /plantranslab/gfp_DevSB.htm (157 words)

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