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Effect of GMCSF of the pharmacokinetics of indinavir in HIV-infected patients. Effect of GMCSF of the pharmacokinetics of indinavir in HIV-infected patients. GMCSF increases the number of white blood cells and stimulates both granulocyte and macrophage function. Coadministration of subcutaneous GMCSF did not change IDV pharmacokinetics and routine dose adjustment of IDV is not necessary. gateway.nlm.nih.gov /robot_pages/MeetingAbstracts/102187975.html   (267 words)

David Golde   (Site not responding. Last check: 2007-10-11) This tiny amount of GMCSF took ten years to isolate, and one rule of protein chemistry is that if you get two proteins on a gel, the one you're interested in will be the smaller one. The GMCSF molecule binds to its specific receptor, setting up a signal which regulates the expression of new genes in the target cell. Experimentally we gave GMCSF and surprisingly we found that the white blood cellcount began to rise; it rose from a level of zero up to many thousands, an as soon as the neutrophil count began to rise, the patient's fever went away and blood pressure returned to normal. www.accessexcellence.org /AB/CC/goldepres.html   (2776 words)

A 59 year old man comes to the ER because of fever, shaking GMCSF: (granulocyte-macrophage colony-stimulating factor) is indicated in bome marrow insufficiency caused by anti neoplasm therapy. GMCSF elicits these functions by activating a receptor consisting of a ligand-specific -chain and a -chain, which is common for GMCSF, IL-3 and IL-5. Using a yeast two-hybrid system, we identified the -chain of the GMCSF receptor as putative interaction partner of IB kinase, one of the central signaling kinases activating the transcription factor NF-B. Using endogenous protein levels of endothelial cell extracts, we could verify the interaction by co-immunoprecipitation experiments. www.aippg.net /forum/viewtopic.php?t=45930   (555 words)

Gourlay, et al., Endotoxin Tolerance Is Reversed By Granulocyte Macrophage Colony-Stimulating Factor (GMCSF)   (Site not responding. Last check: 2007-10-11) GMCSF is known to prime the naive macrophage response to LPS stimulation leading to an excessive response which may be deleterious. To test this we studied the effect of GMCSF in endotoxin tolerant macrophages as an in vitro model of immunoparalysis. GMCSF significantly reverses the inhibition of both p38 and ERK1/2 activation in tolerant cells. www.aast.org /01abstracts/01absPoster_130.html   (346 words)

CpG DNA: A potent signal for growth, activation, and maturation of human dendritic cells -- Hartmann et al. 96 (16): ... Dendritic precursor cells were incubated for 48 h in the presence of GMCSF (800 units/ml) and the CpG phosphorothioate oligonucleotide (2 µg/ml) as indicated. Remaining cells were incubated with GMCSF (800 units/ml) and the CpG oligonucleotide 2006 (2 µg/ml) as indicated. DCs were incubated with GMCSF (800 units/ml) or the CpG oligonucleotide 2006 (2 µg/ml) as indicated. www.pnas.org /cgi/content/full/96/16/9305   (5161 words)

Untitled Document Since 1997 GMCSF has been regularly used as Hepatitis B adjuvant in Dialysis population at this centre. GMCSF was administered as adjuvant at the dose of 150 mcg a day prior to Hep B Vaccine. However, in two patients, despite 5 doses of Hep B vaccine and GMCSF adjuvant, protective levels of anti Hbs titres were not achieved. www.ijnephrol.com /julsep2001/page133.htm   (764 words)

Tertiary Structure   (Site not responding. Last check: 2007-10-11) Unlike IL-4, there is a fairly large patch of hydrophobic residues on one side of the protein surface. It is interesting to note that most of the Prolines in the GMCSF structure are all located on one side of the molecule surface, mostly in the loop regions and thus are exposed to some degree, except one is in one of the helices and buried. It is clear from the above table that not all the "conserved" Leucines are located in helix because the various helices of the different cytokines have different lengths. www.cryst.bbk.ac.uk /PPS2/projects/kingman/pps_proj/TS.html   (452 words)

GMCSF activates NF-{kappa}B via direct interaction of the GMCSF receptor with I{kappa}B kinase {beta} -- Ebner et al. ... GMCSF activates NF-{kappa}B via direct interaction of the GMCSF receptor with I{kappa}B kinase {beta} -- Ebner et al. HeLa cells expressing the fluorescent fusion proteins were treated with GMCSF and subjected to FRET microscopy as described in "Materials and methods." Specific bleaching of the acceptor fluorophore (EYFP) resulted in a significant increase of the donor fluorescence (ECFP) exactly at the sites of colocalization. B inhibition or GMCSF withdrawal on the proliferation of TF-1 cells. www.bloodjournal.org /cgi/content/full/102/1/192   (5304 words)

Leukemia Insights Newsletter   (Site not responding. Last check: 2007-10-11) Using GMCSF, the normal stem cells might be stimulated to proliferate and would have a growth advantage in the control of hematopoiesis. This pilot trial suggested that the addition of GMCSF to the treatment with IFN-A in CML patients who are sensitive to IFN-A alone but fail to achieve a major cytogenetic response may be beneficial in some patients and should be further investigated. The duration of therapy with GMCSF will be a minimum of 6 months (2 cytogenetic evaluations at 3 month intervals) to be evaluable for response and no more than 1 year in those not showing improvement in cytogenetic response. www3.mdanderson.org /leukemia/insight/letter43.html   (3785 words)

ARS | Publication request: Efficient Transient Expression of Human Gm-Csf Protein in Nicotiana Benthamiana Using Potato ... Infected plants accumulated GMCSF at up to 2% of total soluble protein, and this protein showed native protein activity in a cell proliferation bioassay. GMCSF has important medical applications, including treatment of bone marrow transplant recipients. Production of GMCSF in plants may offer a safe and economical alternative means of producing pharmaceutical proteins while providing increased income opportunities for farmers. www.ars.usda.gov /research/publications/publications.htm?seq_no_115=164407&pf=1   (402 words)

INTRODUCTION Reovirus is a double stranded RNA virus(sherry7) that causes many diseases in mice including lyphoblastic ...   (Site not responding. Last check: 2007-10-11) The cytokine, granulocyte-macrophage colony stimulating factor (GMCSF) plays an important role in the immune response to pathogens. Granulocyte-macrophage colony-stimulating factor (GMCSF) is a cytokine that stimulates the growth and differentiation of granulocytes, monocytes, erythrocytes, and megakaryocytes (2). C in 5% CO After given incubation periods, the tubes were centrifuged for 2 minutes at 1100 x g; the supernatant was retained and the pellet discarded. www.mtsu.edu /~scientia/journals/vol4/issue2/gmcsfscientia.htm   (1437 words)

Clinical Trial Process   (Site not responding. Last check: 2007-10-11) The patient, then for the next several days at home, would inject for themselves additional doses of Leukine locally where the vaccine was administered. In this setting, it is used to increase the immune response to the vaccine]. The purpose of the GMCSF is to help the immune system to respond in that area and to try and stimulate the immune system to get in a more strong fashion against the tumor. www.healthtalk.com /lymphoma/programs/050903/page04.cfm   (1314 words)

Melanoma vaccine shows promise Responses by the immune system's killer or cytotoxic t-cells were observed in the lymph nodes draining the vaccine site in 80 percent of patients on the GMCSF arm, and in the peripheral blood of 42 percent of the GMCSF patients. However, t-cell responses were observed in the lymph nodes of only 13 percent of the patients vaccinated with dendritic cells and 11 percent of those patients' peripheral blood. Slingluff and the team of U.Va. scientists have kept the GMCSF arm of the melanoma vaccine trial open, and have offered booster vaccines to some patients with evidence of tumor regression or stable disease. www.medicalnewstoday.com /medicalnews.php?newsid=4589   (617 words)

Delayed Neutrophil Apoptosis in Bovine Subclinical Mastitis -- Boutet et al. 87 (12): 4104 -- Journal of Dairy Science Cells were then stimulated with GMCSF for 20 min before protein extraction was performed and STAT5 DNA-binding activity analyzed by electrophoretic mobility shift assay. Cells from mastitis cows were cultured in the presence or absence of GMCSF (open: untreated; hatched: treated). Enhanced survival of lung granulocytes in an animal model of asthma: Evidence for a role of GMCSF activated STAT5 signalling pathway. www.dairy-science.org /cgi/content/full/87/12/4104   (4850 words)

MEDICINE In terms of absolute numbers of patients treated, the most frequent current use of GCSF and GMCSF is to promote the regeneration of granulocytic and monocytic cells in cancer patients who have received prior chemotherapy (62, 63). Worthy of further exploration is the use locally of GMCSF to promote wound and chronic ulcer healing. The powerful ability of GMCSF to stimulate dendritic cell function (66) and thus to improve the effectiveness of vaccines involving weak antigens (67, 68) needs to be properly exploited. www.leadershipmedica.com /0600/INGLESE/6meteng7.htm   (703 words)

Peroxisome Proliferator-activated Receptor {gamma} Ligands Regulate Myeloperoxidase Expression in Macrophages by an ... The expression level in the absence of RS is designated 0, with -fold induction or repression because of RS treatment indicated. In GMCSF macrophages, MPO immunostaining was abundant in the in MCSF and the dominance of corepressors in GMCSF macrophages. www.jbc.org /cgi/content/full/279/9/8300   (7509 words)

BIOVEX SUBMITS IND FOR CLINICAL TRIAL OF ONCOVEX GM-CSF IN MALIGNANT MELANOMA It also induces tumour cells to secrete GMCSF, a powerful immune stimulator, potentially enhancing the destruction of both injected and metastatic tumour deposits. “We believe the combination of oncolytic activity with the effects of GMCSF on the immune system could be potentially useful in treating a number of solid tumours. BioVex was selected by the American Association for Cancer Research (AACR) to present details on its recent OncoVEX GMCSF Phase I/II clinical trial in a press briefing held on April 19 during the Association's 96 th Annual Meeting in Anaheim, CA. www.biovex.com /04_05_05.html   (593 words)

GCSF GCSF and GMCSF are types of growth factors that stimulate the bone marrow (where blood cells are made) to make the different types of blood cells. Growth factors are special proteins which are produced naturally in the body. GMCSF may cause fever, chills and fluid retention. home.earthlink.net /~ct66/text/GCSF.html   (672 words)

Effects of GMCSF and HIV-1 infection on the expression of CD11a on monocyte-derived macrophages. Effects of GMCSF and HIV-1 infection on the expression of CD11a on monocyte-derived macrophages. Percent positive cells and equivalent molecules of fluorescence (EMF) as determined by calibration beads, were quantitated by flow cytometry. CONCLUSION: Both GMCSF and HIV-1 appear to upregulate surface expression of CD11a on Mphi. www.aegis.com /aidsline/1994/dec/M94C4194.html   (441 words)

Untitled Document Prospective randomised trial of gmcsf as adjuvant for hepatitis B vaccination in CRF GmCSF is an important adjuvant for increasing seroconversion (SC) rate following hepatitis B vaccination (HBVa) in CRF. In this prospective randomised trial, 60 patients of CRF (negative for HBsAg and anti-HBs) were planned to be included; 20 without GmCSF (Gr-A), 20 with GmCSF with first dose of HBVa (Gr-B) and 20 with first and second dose of HBVa (Gr-C). www.ijnephrol.com /julsep2001/page102.htm   (716 words)

BIOVEX'S ONCOVEX GM-CSF CLINICAL TRIAL DATA TO BE FEATURED BY AMERICAN ASSOCIATION FOR CANCER RESEARCH AT 96th ANNUAL ... It is the only product in clinical development that combines an oncolytic effect while inducing tumor cells to secrete GMCSF, a powerful immune stimulator, potentially enhancing the destruction of metastatic tumor deposits. OncoVEX GMCSF has completed a Phase I/II study in several solid tumours including breast cancer, head and neck cancer, gastro-intestinal cancer and melanoma. Results from the administration of OncoVEX GMCSF in either single or multiple doses have shown the product to be well tolerated. www.biovex.com /19_04_05.html   (559 words)

Biovex begins dosing patients in phase II clinical trials of Oncovexgmcsf in malignant melanoma and in head and neck ...   (Site not responding. Last check: 2007-10-11) The end point of the trial is to assess the safety of OncoVEX GMCSF combined with chemoradiotherapy, and the potential of OncoVEX as neoadjuvant therapy in the treatment of head and neck cancer. OncoVEX GMCSF is an oncolytic virus that selectively kills tumor cells. OncoVEX GMCSF has completed a Phase I/II study in several solid tumors including breast cancer, head and neck cancer, gastro-intestinal cancer and melanoma. www.pipelinereview.com /joomla/content/view/887/104   (826 words)

Table 4   (Site not responding. Last check: 2007-10-11) This array accommodated 4 different factors (MCSF, IL-3, GMCSF, and GCSF) each at 2 different concentrations (see above). 8 experimental runs were carried out according to the combination of factors in the array, for example, in experimental run 1 the MTT assay was carried out after mixing the cells with 100 U/ml MCSF, 10 ng/ml IL-3, 10 ng/ml GMCSF, and 10 ng/ml GCSF. The interaction between MCSF and the other three factors (IL-3, GMCSF and GCSF) was studied in this array. www.issoonline.com /content/1/1/7/table/T4   (81 words)

Cancer Vaccine - GMCSF gene modified tumor vaccine shows promise in advanced... GMCSF gene modified tumor vaccine shows promise in advanced lung cancer A GMCSF gene modified tumor vaccine has shown promise for the treatment of advanced stage non-small cell lung cancer. According to a study from the United States, "Treatment for advanced stage non-small cell lung cancer is limited. www.newsrx.com /newsletters/Drug-Week/2003-11-21/11212003333757W.html   (324 words)

Granulocyte-Macrophage Colony-stimulating Factor Gene Transcription Is Directly Repressed by the Vitamin D3 Receptor. ... the GMCSF element as an apparent monomer in vitro. GMCSF is a glycoprotein that signals through a cell surface receptor of the hematopoietin receptor family. The primary role of GMCSF is to activate mature granulocytes www.jbc.org /cgi/content/full/273/17/10338   (7680 words)

DAVID: Database for Annotation, Visualization, and Integrated Discovery (Laboratory of Immunopathogenesis and ... , GM-CSF induced human myelopoiesis is regulated by transforming growth factor-beta isoforms, GMCSF is induced in bronchial epithelial cells by the MAP kinase signaling system in response to air pollutants, GM-CSF plays a critical role in lung homeostasis by regulating levels of MCSF, MCP-1 and IL-8. , Incubation of eosinophils with GMCSF leads to reduced expression of IL-5R alpha that is sustained for up to 5 days; in contrast, eosinophil IL-3R alpha expression is increased by GMCSF, whereas eosinophil GMCSF receptor alpha is down-regulated by GMCSF. , GMCSF was highly expressed in dendritic cells with a bicistronic retroviral vector. david.abcc.ncifcrf.gov /geneReportFull.jsp?rowids=,2871241   (1214 words)

AUUUA Sequences Direct mRNA Deadenylation Uncoupled from Decay during Xenopus Early Development -- Voeltz and Steitz 18 ... The mammalian GMCSF ARE directs mRNA deadenylation during Xenopus early development. The amount of GMCSF transcript remaining divided by the amount of coinjected control remaining for each time point was plotted as a function of time. The amount of GMCSF transcript remaining divided by the amount of coinjected control Xmyc-M2 remaining was plotted as a function of time. mcb.asm.org /cgi/content/full/18/12/7537   (6447 words)

Diphtheria toxin-murine granulocyte-macrophage colony-stimulating factor-induced hepatotoxicity is mediated by Kupffer ...   (Site not responding. Last check: 2007-10-11) GMCSF, a fusion toxin composed of the NH -terminal region In toxin-sensitive cells, DT GMCSF binds to and is internalized Tumor cells were treated for 72 hours with the indicated doses of fusion toxins and cytotoxicity was assessed by measuring the incorporation of radiolabeled thymidine by viable cells during the last 18 hours of culture. mct.aacrjournals.org /cgi/content/full/3/12/1681   (4793 words)

Transfer of Granulocyte-Macrophage Colony-stimulating Factor Gene to Rat Lung Induces Eosinophilia, Monocytosis, and ... Granulocyte-macrophage colony-stimulating factor (GMCSF) is a pleiotropic cytokine whose expression is increased in numerous Increased amounts of GMCSF are also found in BAL of patients with It is noteworthy that the fibrotic response we observed www.jci.org /cgi/content/full/97/4/1102   (5473 words)

Development of mouse-specific contraceptive vaccines: infertility in mice immunized with peptide and polyepitope ... SP56, GMCSF and PRL, and only 40% of mice in each of these groups Individual or groups of peptides were either keyhole limpet hemocyanin (KLH) conjugates or fused to a 6XHis tag (peptide ZP1). SP56 (S + G + P), GMCSF (S + G + P) and PRL (S + G + P) indicate response to SP56, GMCSF and PRL respectively from mice immunized with mixed peptides SP56, GMCSF and PRL. www.reproduction-online.org /cgi/content/full/128/4/395   (5603 words)

human GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) antibody: purified antibody Source: E. coli expressed amino acids ala 18 - glu144 of mature human GMCSF (accession # NM_000758). Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 14,478. Carrier-Free Recombinant Human GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) www.ebioscience.com /ebioscience/specs/antibody_14/14-8339.htm   (377 words)

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