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	Reference.com/Encyclopedia/Giemsa stain
		Giemsa stain, named after Gustav Giemsa, an early malariologist, is used for the histopathological diagnosis of malaria and other parasites.
		Giemsa stain is used in Giemsa banding, commonly called G-banding, to stain chromosomes and often used to create a karyotype.
		Giemsa stain is a classical blood film stain for peripheral blood smears and bone marrow specimens.
	www.reference.com /browse/wiki/Giemsa (304 words)

	NationMaster - Encyclopedia: Giemsa (Site not responding. Last check: )
		Giemsa staining produced heavy bands in the centromeric and pericentromeric regions of chromosome II and the X chromosome, as well as one band on the centromere of chromosome III, and a stained block outside the centromeric region.
		The Giemsa stained blocks in the pericentromeric region of chromosomes II, III and X disappeared after C-banding, demonstrating that they are not formed from constitutive heterochromatin.
		The Y chromosome, that appeared totally heterochromatic with Giemsa staining, presented an intensely stained band in the centromeric region after C-banding and was sub-metacentric (Figure 2).
	www.nationmaster.com /encyclopedia/Giemsa (288 words)

	Plastic Embedding from Energy Beam Sciences
		This Giemsa stain works well for a variety of tissues.
		Stain in Giemsa working solution at room temperature for 1.5 hours.
		Rinse in dilute acetic acid (4 drops in 100 ml.
	www.ebsciences.com /histology/giemsag.htm (91 words)

	In silico chromosome staining: Reconstruction of Giemsa bands from the whole human genome sequence -- Niimura and ...
		In silico chromosome staining: Reconstruction of Giemsa bands from the whole human genome sequence -- Niimura and Gojobori, 10.1073/pnas.022437999 -- Proceedings of the National Academy of Sciences
		In silico chromosome staining: Reconstruction of Giemsa bands from the whole human genome sequence
		Giemsa staining has been used for identifying individual human chromosomes.
	www.pnas.org /cgi/content/abstract/022437999v1 (414 words)

	Giemsa banding of diploid perennial teosinte and its hybrids with maize (Site not responding. Last check: )
		Giemsa banding of diploid perennial teosinte and its hybrids with maize
		It was consistently found that the bands corresponded in shape, number and size with the knobs appearing on the pachytene chromosomes stained with conventional techniques.
		Therefore, it is conceivable that Giemsa banding has indeed great potential in both the study of variations of plant karyotypes and genetic research in general.
	www.maizegdb.org /mnl/57/23ting.html (620 words)

	Giemsa_stain - The Wordbook Encyclopedia
		Erythrocytes stain pink, platelets show a light pale pink, lymphocyte cytoplasm stains sky blue, monocyte cytoplasm stains pale blue, and leukocyte nuclear chromatin stains magenta.
		Giemsa stain is also used to visualize chromosomes.
		A thin film of the specimen on a microscope slide is fixed in absolute methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide.
	www.thewordbook.com /Giemsa_stain (285 words)

	Giemsa stain Summary
		The Giemsa stain is one of the larger group of Romanowsky stains.
		Giemsa stain is used for the histopathological diagnosis of Malaria and other parasites.
		Giemsa stain is a mixture of methylene blue and eosin.
	www.bookrags.com /Giemsa_stain (0 words)

	In silico chromosome staining: Reconstruction of Giemsa bands from the whole human genome sequence -- Niimura and ...
		Giemsa bands are shown in fl (Top) and in silico bands are shown in blue (Middle).
		Giemsa and in silico bands for all chromosomes.
		Giemsa bands obtained from Francke (14) are shown in fl ideograms.
	www.pnas.org /cgi/content/full/022437999v1 (0 words)

	Giemsa Stain Recipe
		Dissolve 3.8g of Giemsa powder into 250ml of methanol
		Giemsa stain is used on blood and bone marrow.
		Erythrocytes stain pink, platelets a very light pink, leukocyte nuclear chromatin stains magenta, monocyte cytoplasm stains pale blue and lymphocyte cytoplasm stains sky blue.
	www.thelabrat.com /protocols/GiemsaStain.shtml (104 words)

	Evaluation of the polymerase chain reaction analysis for diagnosis of falciparum malaria in Delhi, India Nandwani S, ...
		PCR analysis for P. falciparum from venous blood and at the same time Giemsa staining of thick and thin blood smears was done.
		Giemsa staining was time consuming, laborious and may give poor results in cases with low parasitaemia.
		PCR analysis can be used to supplement the conventional Giemsa staining for reliable diagnosis of falciparum malaria especially in cases with low parasitaemia.
	www.ijmm.org /article.asp?issn=0255-0857;year=2005;volume=23;issue=3;spage=176;epage=178;aulast=Nandwani;type=0 (298 words)

	DPDx Diagnostic Procedures for Blood Specimens - Staining
		Kept tightly stoppered and free of moisture, stock Giemsa stain is stable at room temperature indefinitely (stock stain improves with age).
		Note: As alternates to this 45-60 minutes in 2.5% Giemsa stain, the smears could be stained for shorter times in more concentrated stains.
		One alternate is 10 minutes in 10% Giemsa; the shorter stains yield faster results, but use more stain and might be of less predictable quality.
	www.dpd.cdc.gov /dpdx/HTML/Frames/DiagnosticProcedures/body_dp_bloodstaining.htm (560 words)

	The Scientist : chromosome staining
		The chromosomal bands observed upon Giemsa staining are thought to correspond generally to regions that are GC-poor (Giemsa-dark, G bands) and GC-rich (Giemsa-light, R bands).
		In the January 22 Proceedings of the National Academy of Sciences, Niimura and Gojobori describe a computational method to explore the association between the Giemsa banding pattern and local GC content (Proc Natl Acad Sci USA 2002, 99:797-802).
		Niimura Y and Gojobori T: In silico chromosome staining: Reconstruction of Giemsa bands from the whole human genome sequence.
	www.the-scientist.com /article/display/20160 (179 words)

	Bioline International Official Site (site up-dated regularly)
		Background: Modified Giemsa staining has been favoured by many researchers because it is easy to perform but, like many other stains, demonstration of the bacteria depends on its morphology.
		In 2 cases immunostain could not demonstrate the bacteria but they were identified with modified Giemsa stain while in 5 cases the bacteria were identified by immunostain but not with modified Giemsa stain.
		The positive predictive value of modified Giemsa stain was 93% CI 75 -98.8%) while the negative predictive value was 74% (CI 48.6 - 89.9).
	www.bioline.org.br /abstract?id=hs02039&lang=en (319 words)

	Chapter 8
		Blood cells arrested in metaphase were stained with Giemsa and photographed with a microscope.
		Giemsa is a stain that stains chromosomal DNA.
		Giemsa stains A-T rich areas of a chromosome more intensely than G-C rich areas of a chromosome.
	pages.cabrini.edu /ddunbar/BIO263/chapter_9.htm (1214 words)

	Conventional Giemsa Stain 2 (Site not responding. Last check: )
		Conventional staining techniques are used to uniformly stain chromosomes and leave the centromeres constricted, thus enabling the measurement of chromosome length, centromeric position, and arm ratio.
		Prior to 1960, when Moorehead and Nowell described the use of Giemsa in their chromosome preparations, conventional cytologic stains such as acetoorcein, acetocarmine, gentian violet, hematoxylin, Leishman's, Wright's, and Feulgen stains were used to stain chromosomes.
		Giemsa stain is now the most popular stain for chromosome analysis (Gustashaw, 1991).
	homepage.mac.com /wildlifeweb/cyto/text/GiemsaStain2.html (183 words)

	Cien anos del colorante de Giemsa. (Site not responding. Last check: )
		Research work associated with the development of Giemsa stain is revived, with emphasis on the methylene blue polychromes.
		A short biographical sketch of Dr. Berthold Gustav Carl Giemsa is presented, along with the composition of his original formulation and its mec.
		Applications of Giemsa stain reported in the last 32 years are tabulated.
	www.infodoctor.org /cgi-bin/abstracts.pl?uid=12696395 (150 words)

	Validez de las tinciones de Giemsa y Lendrum en frotis conjuntivales para la identification de Chlamydia trachomatis. (Site not responding. Last check: )
		With the objective of finding reliable, valid, and economic diagnostic tests to identify Chlamydia trachomatis in conjunctival smears, the sensitivity, specificity, and positive and negative predictive values of Lendrum and Giemsa stains were evaluated using direct immunofluorescence as the gold standard.
		The sensitivity of the Giemsa stain was a bit higher than that of the Lendrum stain (28% and 22%, respectively), and the specificity was similar (82% and 85%, respectively).
		The Lendrum and Giemsa stains are not adequate tests for the diagnosis of ocular chlamydiosis.
	www.infodoctor.org /cgi-bin/abstracts.pl?uid=7518685 (267 words)

	Chlamydia, Lab Diagnostic Testing, The Charles T. Campbell Eye Microbiology Lab, UPMC \| University of Pittsburgh ...
		The giemsa stain is used to examine conjunctival specimens for cytology and microorganisms.
		Although the giemsa stain is a powerful tool, it rarely is offered because of the lack of experienced personnel.
		Giemsa stain of Chlamydia inclusion bodies (purple "caps" on epithelial cell).
	eyemicrobiology.upmc.com /Chlamydia.htm (533 words)

	Cytology, Lab Diagnostic Testing, The Charles T. Campbell Eye Microbiology Lab, UPMC \| University of Pittsburgh Medical ...
		Gram stain is used to examine for bacteria, and the giemsa stain is used to determine cytology.
		The giemsa stain is also excellent for detecting bacteria, fungus, chlamydia, and acanthamoeba.
		Many laboratories are uncomfortable with the giemsa stain because of limited experience.
	eyemicrobiology.upmc.com /Cytology.htm (479 words)

	Navy Medical Department Guide to Malaria Prevention and Control: Appendix 2: Mosquito Vectors and Identification
		If using the one slide method, prevent exposure of the thick smear to methanol or methanol fumes by carefully dipping or dabbing the slide, and gently blowing the fumes away from the thick smear area.
		Giemsa stain is available in the military supply system, and this staining method is presented.
		Preparation of Giemsa staining solution is done with buffered water and Giemsa concentrate.
	www.brooksidepress.org /Products/OperationalMedicine/DATA/operationalmed/Manuals/malaria/App3.html (1395 words)

	A field guide for THE DIAGNOSIS, TREATMENT AND PREVENTION OF AFRICAN ANIMAL TRYPANOSOMOSIS
		Giemsa staining, after fixation by methanol, gives the best result, but it takes more time than more recent fast stains.
		Buffered water should not be kept for long periods before use and if at all it should be stored in a dark bottle, to avoid the growth of algae (which may even be the cause of pseudo-parasites in stained smears).
		Mix 3.8 g of Giemsa powder with 250 ml of pure glycerol and 250 ml of pure methanol.
	www.fao.org /DOCREP/006/X0413E/X0413E07.htm (2028 words)

	New Methods for Karyotyping (Site not responding. Last check: )
		Different dyes that affect different areas of the chomosomes are used for a range of identification purposes.
		One common dye used is Giemsa; That process is known as G-banding (see the G-banded chromosomes in the image to the left).
		This dye is effective because it markedly stains the bands on a chromosome; Each chromosome can then be identified by its banding pattern, but the resuls is similar overall gray values for each chromosome.
	www.biology.arizona.edu /human_bio/current/new_karyotyping/new_karyotyping.html (322 words)

	Cucurbit Genetics Cooperative - CGC Report No. 8:13-14 (1985)
		However, the genes have not been assigned to any of the chromosomes, which are not distinguishable by normal staining procedures.
		Modern staining techniques like Giemsa C-banding has facilitated the identification of individual chromosomes in many plant and animal species (5).
		Giemsa C-banded mitotic chromosomes and idiogram of C.
	cuke.hort.ncsu.edu /cgc/cgc08/cgc8-6.html (415 words)

	The Science Advisory Board - Protocols, Product Reviews, Member Forum, and Science News
		Stain in a mixture of 1ml of Giemsa stock plus 2mls of pH6.4 buffer and 47mls of distilled water - 40 to120 minutes.
		Stain in a coplin jar in a mixture of 1mls of Giemsa stock plus 45mls distilled water in a water bath at 56oC - 20 mins to 1 hour (maximum).
		Mix 7.36g Giemsa powder in 500mls glycerol which is heated to 50oC in a water bath.
	www.scienceboard.net /resources/protocols.asp?action=article&protocol_id=311&criteria= (379 words)

	4.2.2.2 (Site not responding. Last check: )
		In the case of Giemsa, the staining solution is often poured below the slide (rather than on the slide) to avoid stain precipitates [Photograph: Marcel Hommel]
		Giemsa is a mixture of eosin and methylene blue.
		Stock solutions of Giemsa stain must always be diluted by mixing an appropriate amount of stain with distilled neutral or slightly alkaline water; buffered saline is preferred because it provides a cleaner background and better preservation of parasite morphology.
	www.impact-malaria.com /FR/EPS/Formations_et_cours_internationaux/Formation_de_la_Liverpool_School_LSTMH/cours_liverpool/Unit_4/4_2_2_2.html (294 words)

	In silico chromosome staining: Reconstruction of Giemsa bands from the whole human genome sequence -- Niimura and ... (Site not responding. Last check: )
		In silico chromosome staining: Reconstruction of Giemsa bands from the whole human genome sequence -- Niimura and Gojobori 99 (2): 797 -- Proceedings of the National Academy of Sciences
		Giemsa staining has been used for identifying individual human
		Giemsa bands computationally from the whole human genome sequence.
	intl.pnas.org /cgi/content/abstract/99/2/797 (440 words)

	[No title] (Site not responding. Last check: )
		Giemsa stain is prepared with water buffered to pH 7.2 and Giemsa concentrate.
		Giemsa powder requires too much work to dissolve properly.
		Prepare Giemsa by mixing: 1 part Giemsa concentrate with 9 parts of buffered water (pH 7.2).
	www.pitt.edu /~super1/lecture/lec10251/029.htm (291 words)

	Histology methods - Giemsa for blood cells and parasites (Site not responding. Last check: )
		Stain in a mixture of 1ml of Giemsa stock plus 2mls of pH6.4 buffer and 47mls of distilled water - 40 to120 minutes.
		Place in a Coplin jar containing Giemsa stain diluted 1:10 with pH 6.8 buffer for 20 minutes.
		Mix 7.36g Giemsa powder in 500mls glycerol which is heated to 50
	www.bristol.ac.uk /vetpath/cpl/giemsa.html (358 words)

	Definition of Giemsa stain - Merriam-Webster Online Dictionary
		Learn more about "Giemsa stain" and related topics at Britannica.com
		Find more about "Giemsa stain" instantly with Live Search
		See a map of "Giemsa stain" in the Visual Thesaurus
	www.webster.com /dictionary/Giemsa+stain (61 words)

	BioMed Central \| Full text \| Atypical Herpes simplex keratitis (HSK) presenting as a perforated corneal ulcer with a ...
		Gram, Giemsa stained smears and the potassium hydroxide preparation of the scraping did not reveal any organisms.
		However, Giemsa stained smear showed the presence of multinucleated giant cells, with characteristic molding of nuclei (Fig.
		This case was diagnosed initially as bacterial keratitis considering the rapid progression of the symptoms and a history of contact lens wear.
	www.biomedcentral.com /1471-2415/1/1 (1449 words)

	Figures and Tables (Site not responding. Last check: )
		Time of infection: 160 days (Lennert's Giemsa, X 200).
		Time of infection: 160 days (Lennert's Giemsa, X 100).
		Time of infection: 90 days (Lennert's Giemsa, X 100).
	www.scielo.br /img/fbpe/mioc/v93s1/html/212.html (151 words)

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