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Topic: Giemsa stain

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	Richard-Allan Scientific : Hematology
		Giemsa stain is an excellent classical blood film stain used on peripheral blood smears and bone marrow.
		Erythrocytes stain pink with platelets exhibiting a very light, pale hue.
		Leukocyte nuclear chromatin stains magenta-purple, monocyte cytoplasm stains the classical âground glassâ blue and lymphocyte cytoplasm stains clear, sky blue.
	www.rallansci.com /hematology/hematology.aspx?id=83 (48 words)

	Giemsa Stain Recipe
		Dissolve 3.8g of Giemsa powder into 250ml of methanol
		Giemsa stain is used on blood and bone marrow.
		Erythrocytes stain pink, platelets a very light pink, leukocyte nuclear chromatin stains magenta, monocyte cytoplasm stains pale blue and lymphocyte cytoplasm stains sky blue.
	www.thelabrat.com /protocols/GiemsaStain.shtml (0 words)

	Giemsa stain Summary
		Giemsa stain is used for the histopathological diagnosis of Malaria and other parasites.
		Giemsa stain is a classical blood film stain for peripheral blood smears and bone marrow specimens.
		Giemsa stain is a mixture of methylene blue and eosin.
	www.bookrags.com /Giemsa_stain (423 words)

	NationMaster - Encyclopedia: Giemsa (Site not responding. Last check: )
		Giemsa staining produced heavy bands in the centromeric and pericentromeric regions of chromosome II and the X chromosome, as well as one band on the centromere of chromosome III, and a stained block outside the centromeric region.
		The Giemsa stained blocks in the pericentromeric region of chromosomes II, III and X disappeared after C-banding, demonstrating that they are not formed from constitutive heterochromatin.
		The Y chromosome, that appeared totally heterochromatic with Giemsa staining, presented an intensely stained band in the centromeric region after C-banding and was sub-metacentric (Figure 2).
	www.nationmaster.com /encyclopedia/Giemsa (307 words)

	Reference.com/Encyclopedia/Giemsa stain
		Giemsa stain is a classical blood film stain for peripheral blood smears and bone marrow specimens.
		Erythrocytes stain pink, platelets show a light pale pink, lymphocyte cytoplasm stains sky blue, monocyte cytoplasm stains pale blue, and leukocyte nuclear chromatin stains magenta.
		Giemsa stain is also used to visualize chromosomes.
	www.reference.com /browse/wiki/Giemsa_stain (250 words)

	giemsa - Definition and Meaning of giemsa
		Giemsa stain is used to differentiate nuclear and/or cytoplasmic morphology of...
		Giemsa stain (gms stn) (chemistry) A stain for hemopoietic tissue and...
		Giemsa stain is used for the histopathological diagnosis of Malaria and...
	www.wordiq.com /reference/giemsa (390 words)

	CyberTec - Product Lines (Site not responding. Last check: )
		Staining according to Pappenheim enables to carry out blood and bone-marrow differential cell counting.
		May-Grünwald stains acidophilic cells and the neutrophilic granulations of leukocytes, whereas Giemsa stains the cytoplasm of monocytes and lymphocytes, as well as nuclear chromatin.
		Use of Armand's stain enables simultaneous discolouration of all bacteria, except mycobacteria, and the colouration of the background of the preparation.
	www.mrlcybertec.com /products/reactifs.html (226 words)

	Reference.com/Encyclopedia/Wright's stain
		Wright's stain is a technique in histology that is used to make the differences between cells visible under light microscopy.
		Wright's stain is also used in cytogenetics to stain chromosomes on slides for visualization and diagnosis of sydromes and disease.
		There are related stains known as the buffered Wright stain, the Wright-Giemsa stain, and the buffered Wright-Giemsa stain, and specific instructions depend on the solutions being used, which may include Eosin Y, Azure B, and Methylene Blue (some commercial preparations combine solutions to simplify staining).
	www.reference.com /browse/wiki/Wright%27s_stain (224 words)

	Giemsa_stain - The Wordbook Encyclopedia
		It can be used for histopathological diagnosis of malaria and some other spirochete and protozoan blood parasites.
		Giemsa stain is a mixture of methylene blue and eosin.
		A thin film of the specimen on a microscope slide is fixed in absolute methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide.
	www.thewordbook.com /Giemsa_stain (285 words)

	Bioline International Official Site (site up-dated regularly)
		Background: Modified Giemsa staining has been favoured by many researchers because it is easy to perform but, like many other stains, demonstration of the bacteria depends on its morphology.
		In 2 cases immunostain could not demonstrate the bacteria but they were identified with modified Giemsa stain while in 5 cases the bacteria were identified by immunostain but not with modified Giemsa stain.
		The positive predictive value of modified Giemsa stain was 93% CI 75 -98.8%) while the negative predictive value was 74% (CI 48.6 - 89.9).
	www.bioline.org.br /abstract?id=hs02039&lang=en (319 words)

	DPDx Diagnostic Procedures for Other Specimens - Sputum, induced sputum, & bronchoalveolar lavage (BAL)
		Kept tightly stoppered and free of moisture, stock Giemsa stain is stable at room temperature indefinitely (stock stain improves with age).
		Note: As alternates to this 45-60 minutes in 2.5% Giemsa stain, the smears could be stained for shorter times in more concentrated stains; one alternate is 10 minutes in 10% Giemsa; the shorter stains yield faster results, but use more stain and might be of less predictable quality.
		To ensure that proper staining results have been achieved, a positive smear (malaria) should be included with each new batch of working Giemsa stain.
	www.dpd.cdc.gov /dpdx/HTML/Frames/DiagnosticProcedures/body_dp_otherspec_BAL.htm (809 words)

	4.2.2.2 (Site not responding. Last check: )
		Giemsa is a mixture of eosin and methylene blue.
		Stock solutions of Giemsa stain must always be diluted by mixing an appropriate amount of stain with distilled neutral or slightly alkaline water; buffered saline is preferred because it provides a cleaner background and better preservation of parasite morphology.
		The staining solution must be freshly prepared by mixing 5 ml of stock solution with 100 ml of buffered water.
	www.impact-malaria.com /FR/EPS/Formations_et_cours_internationaux/Formation_de_la_Liverpool_School_LSTMH/cours_liverpool/Unit_4/4_2_2_2.html (294 words)

	Bioline International Official Site (site up-dated regularly)
		Modified Giemsa stain was used on subsequent levels of the same blocks by the method described by Gray et al (1996).
		Both immonohistochemical stain and Giemsa stain were positive in 27 cases and negative in 14 cases.
		The modified Giemsa stain provided distinctive shape and uniform staining of the bacteria making their identification easy and this was reflected in a high positive predictive value.
	www.bioline.org.br /request?hs02039 (1737 words)

	Plastic Embedding from Energy Beam Sciences
		This Giemsa stain works well for a variety of tissues.
		Stain in Giemsa working solution at room temperature for 1.5 hours.
		Gerrits, P. and Smid, L., "Staining Procedures for Tissues Embedded in 2-Hydroxyethyl Methacrylate", Heraeus Kulzer.
	www.ebsciences.com /histology/giemsag.htm (0 words)

	Diagnostic Pathology \| Full text \| Mast cell density in gastric biopsies of pediatric age group and its relation to ...
		demonstrated the presence of mast cells by immunohistochemical staining with anti human tryptase antibody in gastric biopsies and concluded that density of mast cells was 2 to 3 times greater in H. pylori infected gastric mucosa than in negative normal stomach [4].
		The specimens were assessed for infection and inflammation and stained with anti-human mast cell tryptase to count mucosal mast cells.
		Although we did not performed immunohistochemical stains for mast cell detection, we propose that Giemsa stain is a reliable and easy staining method available in all laboratories and also has good interobserver correlation.
	www.diagnosticpathology.org /content/2/1/14 (2317 words)

	Chlamydia, Lab Diagnostic Testing, The Charles T. Campbell Eye Microbiology Lab, UPMC \| University of Pittsburgh ...
		The giemsa stain is used to examine conjunctival specimens for cytology and microorganisms.
		Although the giemsa stain is a powerful tool, it rarely is offered because of the lack of experienced personnel.
		Giemsa stain of Chlamydia inclusion bodies (purple "caps" on epithelial cell).
	eyemicrobiology.upmc.com /Chlamydia.htm (533 words)

	atlas
		In a smear of brain tissue, many bradyzoites of Toxoplasma gondii are seen within the cyst (Giemsa stain, 1000x).
		Section of a mouse spleen infected with Toxoplasma gondii tachyzoites multiplying in the cells of the spleen.
		A section of mouse lymph node infected with Toxoplasma gondii tachyzoites multiplying in the cells of lymph node(C)(Giemsa stain, 1000x).
	www.atlas.or.kr /atlas/alphabet_view.php?my_codeName=Toxoplasma%20gondii (86 words)

	Giemsa Staining Kits Procedures for histological staining
		Stain sections in Working May Grunwald stain** for 6 minutes.
		Staining time for the demonstration of Helicobacter Pylori can be reduced to 5 minutes in Working Giemsa Stain**.
		Definition of surrounding tissue may not be as intense using May Grunwald and increased staining times, but target organisms will stain a dark blue to violet.
	www.imebinc.com /IMEB/stainkits/procedures/giemsa.html (271 words)

	GIEMSA STAIN KIT by IMEB INC., your pathology, histology, cytology and dermatology new and refurbished lab equipment ...
		GIEMSA STAIN KIT by IMEB INC., your pathology, histology, cytology and dermatology new and refurbished lab equipment suupliers.
		Giemsa stain kit is used to provide Nuclear and cytoplasm staining in paraffin sections and frozen sections.
		The result is blue to purple nuclei, orange to pink cytoplasm and for H.Pylori; purple staining of the organisms
	www.imebinc.com /Item/K7168.htm (92 words)

	Dako - Giemsa Stain Kit, Artisan™
		The Giemsa stain is a polychromatic stain based on a modified Wohlbach's technique.
		This stain is not intended for staining of bone marrow cores, bone marrow smears, or peripheral blood smears.
		Cell types are stained as follows: mast-cell granules and basophils - purple, eosinophils - bright pink, lymphocytes - blue.
	www.dako.ie /index/prod_search/prod_products.htm?productareaid=17&baseprodidver=A224449005 (130 words)

	Giemsa stain - Genetics Terms and Definitions by Health Dictionary
		Giemsa stain - Genetics Terms and Definitions by Health Dictionary
		Information and definitions of the terms been taken from various reliable government publications and we have done our best to verify their accuracy.
		If you feel any of the definitions are incorrect or needs to be updated please contact us and we will look into it.
	www.sciencedictionary.org /genetics-term-details/Giemsa-stain (67 words)

	Staining Procedures For Plastic Embedded Tissue
		Stain in Giemsa working solution -- 2 hours to overnight.
		Some of the chemicals used for the staining procedures given in this section may be hazardous if misused.
		Note: In order to prevent sections from loosening from the slides during staining, all sections should be heat-fixed (60ºC to 100ºC) to the slides for a minimum of 2-5 minutes prior to staining, preferably at the time the sections are mounted on the slides.
	www.ebsciences.com /staining/giemsa.htm (0 words)

	Definition of Giemsa stain - Merriam-Webster Online Dictionary
		: a stain consisting of eosin and a blue dye and used chiefly in the differential staining of blood films -- called also Giemsa, Giemsa's stain
		Learn more about "Giemsa stain" and related topics at Britannica.com
		Find more about "Giemsa stain" instantly with Live Search
	www.merriam-webster.com /dictionary/Giemsa+stain (53 words)

	48900 Giemsa Stain Modified Solution Standard Fluka, for the staining (of cellular blood components and blood parasites)
		Standard Fluka, for the staining (of cellular blood components and blood parasites)
		Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams and Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.
		When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration.
	www.sigmaaldrich.com /ProductLookup.html?ProdNo=48900&Brand=FLUKA (0 words)

	G5637 Giemsa stain certified by the Biological Stain Commission
		Certified for Giemsa stain for blood films and for the Wolbach's Giemsa method for paraffin sections.
		Stains and Dyes > Certified Stains and Dyes
		Stains and Dyes, A to Z > G-H-I
	www.sigmaaldrich.com /catalog/search/ProductDetail/SIGMA/G5637?CHEMBUYERSGUIDE (0 words)

	Definition of Giemsa stain - Merriam-Webster Online Dictionary
		: a stain consisting of eosin and a blue dye and used chiefly in the differential staining of blood films —called also Giemsa Giemsa's stain
		Learn more about "Giemsa stain" and related topics at Britannica.com
		Find more about "Giemsa stain" instantly with Live Search
	www.webster.com /dictionary/Giemsa+stain (0 words)

	[No title]
		Plasmodium malariae schizonts and gametocytes in peripheral blood, giemsa stain
		Plasmodium vivax trophozoite, schizont and gametocyte in peripheral blood, giemsa stain
		Plasmodium vivax schizont and gametocyte in peripheral blood, giemsa stain
	www.iranhealers.com /attlas/angal.htm (454 words)

	Re: Giemsa Stain (Site not responding. Last check: )
		Try using a 0.5% acetic acid solution to differentiate the Giemsa, instead of the colophonium.
		Or, if you really want to slow down the differentiation, try about 2-4 drops (from an eye dropper) of the acetic acid in a coplin jar of d.
		They > used Giemsa stain on paraffin slides according to the method of McNamara as modified by Putt.
	www.histosearch.com /histonet/Apr99A/Re.GiemsaStain.html (332 words)

	[No title]
		Product Identification Synonyms: Giemsa stain certified; Giemsa's Stain CAS No.: 51811-82-6 Molecular Weight: Not applicable to mixtures.
		Composition/Information on Ingredients Ingredient CAS No Percent Hazardous --------------------------------------- ------------ ------- --------- Giemsa Stain 51811-82-6 90 - 100% Yes 3.
		Disposal Considerations Whatever cannot be saved for recovery or recycling should be managed in an appropriate and approved waste disposal facility.
	www.martellabs.com /msds/01504.txt (1103 words)

	RE: [Histonet] fixative for giemsa stain? (Site not responding. Last check: )
		Although the cells will fix as they stain, with the alcohol in the stain, the right thing to do is to fix in alcohol beforehand.
		My and most others experience is with blood and marrow, but I guess a Giemsa is a Giemsa whatsoever it is staining.
		Dear All I need to do a giemsa stain on some primary muscle cells.
	www.histosearch.com /histonet/Jul04/RE.Histonetfixativeforgie.html (165 words)

	Medical Chemical Corporation
		A solution of giemsa stain and glycerin in methanol.
		MSDS for Giemsa Stain, page 1 of 3
		MSDS for Giemsa Stain, page 2 of 3
	www.med-chem.com /msds/591A.html (630 words)

	Emerson Animal Hospital - Life Cycle
		Life cycle and photomicrographs by Dr. Harold Ray Emerson from original research done in the Department of Veterinary Pathology, College of Veterinary Medicine, Texas AandM University, College Station, Texas, Thesis, 1969.
		A serial study of blood films obtained from infected deer and stained with Giemsa stain or immune conjugate resulted in the differentiation of various developmental stages of Babesia odocoilei.
		The nuclear and cytoplasmic elements of the organisms were also differentiated.
	www.emersonanimalhospital.com /524120.html (384 words)

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