| |
| | Cancer Immunity 5:3 (2005) |
 | | Immune effector function analyses with the Lewis Y antigen system utilised the humanised anti-Lewis Y mAb hu3S193 (35) and IgG1 class-matched control huA33 (36) antibodies produced by the Biological Production Facility (Ludwig Institute for Cancer Research, Melbourne, Australia). |
| | Anti-CD3-FITC/CD4-RPE, anti-CD3-FITC/CD8-RPE, anti-CD19-FITC, anti-CD20-RPE, anti-CD14-RPE, anti-CD15-FITC, mouse IgG1-FITC/IgG1-RPE negative control, mouse IgM-FITC negative control, and mouse IgG2a-RPE negative control were purchased from DAKO (Glostrup, Denmark). |
| | Antibody staining was detected using a secondary biotinylated antibody (LSAB Kit; Dako, Glostrup, Denmark or Silenus, Melbourne, Australia) followed by incubation with horse radish peroxidase conjugated alpha-biotin (LSAB Kit; Dako, Glostrup, Denmark or Vectastain ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA) and visualized by immersing in chromogen substrate AEC (Sigma-Aldrich, St. Louis, MO, USA). |
| www.cancerimmunity.org /v5p3/041221.htm (6756 words) |
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