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Topic: Glucose oxidase

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	Glucose oxidase (Site not responding. Last check: 2007-11-06)
		Since the rate-limiting process in the oxidation of glucose is the electron-transfer between the substrate and the enzyme redox-center, the amperometric response of the electrode is dependant on the glucose concentration.
		Photostimulation of dinitrospiropyran-modified glucose oxidase in the presence of DNP-antibody.
		Enatioselective bioelectrocatalyzed oxidation of glucose oxidase at chiral-monolayer-electrodes.
	chem.ch.huji.ac.il /~eugeniik/glucose_oxidase.htm (3439 words)

	HAO1 - hydroxyacid oxidase (glycolate oxidase) 1
		An electrochemical response of glucose and lactate biosensors which were prepared by coating a platinum electrode with a thin film composed of concanavalin A and mannose-labeled glucose oxidase (GOx) or lactate oxidase (LOx) was evaluated in the presence of ferrocene derivatives as electron mediator.
		Vascular immunotargeting of the H(2)O(2)-generating enzyme glucose oxidase (GOX) to the pulmonary endothelium causes an acute oxidative lung injury in mice.(1) In the present study we compared the pulmonary thrombosis and leukocyte transmigration caused by GOX targeting to the endothelial antigens platelet-endothelial cell adhesion molecule (PECAM) and thrombomodulin (TM).
		HAO1 is a homologue of Hao1 (hydroxyacid oxidase 1, liver) from Mus musculus.
	www.ihop-net.org /UniPub/iHOP/gg/99847.html (945 words)

	Guidance Glucose Document
		DEFINITION: Portable blood glucose devices are intended for use in hospitals, at point of care, in physicians' offices, and over-the-counter as in vitro diagnostic tests for quantitative and semi-quantitative measurement of glucose by glucose oxidase, dehydrogenase, or hexokinase based methodologies.
		Glucose measurements are used in the diagnosis and treatment of carbohydrate metabolism disorders including diabetes mellitus, neonatal hypoglycemia, and idiopathic hypoglycemia, and of pancreatic islet cell tumors.
		These monitors quantify glucose amperometrically by measuring the current that is produced when glucose oxidase catalyzes the oxidation of glucose to gluconic acid or when glucose dehydrogenase catalyzes the oxidation of glucose to gluconolactone.
	www.fda.gov /cdrh/ode/gluc.html (4663 words)

	Diabetes Information - Glucose Meters and Diabetes Management
		The test strips are coated with chemicals (glucose oxidase, dehydrogenase, or hexokinase) that combine with glucose in blood.
		Glucose levels in plasma (one of the components of blood) are generally 10-15% higher than glucose measurements in whole blood (and even more after eating).
		Third-party or "generic glucose reagent strips" are test strips developed as a less expensive option than the strips that the manufacturer intended the meter to be used with.
	www.fda.gov /diabetes/glucose.html (5800 words)

	Cereal Chem 1998 \| Glucose Oxidase Effects on Gluten and Water Solubles (1).
		The objective of this study was to determine whether the H(2)O(2) produced by glucose oxidase affected the gluten proteins or the water-soluble fraction of flour.
		Glucose oxidase had no effect on gluten protein as measured by protein solubility or the relative viscosity of soluble protein (solubilized using 1.5% w/v SDS).
		Glucose oxidase appeared to have the same oxidizing action independent of whether the water-soluble fraction was boiled or not.
	www.aaccnet.org /cerealchemistry/abstracts/1998/1007-04R.asp (252 words)

	Glucose oxidase (Site not responding. Last check: 2007-11-06)
		Glucose oxidase catalyzes the oxidation of (beta)-D-glucose toD-gluconic acid and hydrogen peroxide.
		Although specific for (beta)-D-glucose, glucose oxidase can be used to measure total glucose, because as a result of the consumption of (beta)-glucose, (alpha)-glucose from the equilibrium is converted to the (beta)-form by mutarotation.
		As many glucose assays measure the peroxide produced by the glucose oxidase reaction, it is important that the enzyme used for these assays has a low catalase content in order to avoid destruction of the peroxide and an underestimation ofthe glucose in the assay.
	www.serva.de /products/knowledge/061333.shtml (178 words)

	Glucose oxidase and catalase in the food industry
		Glucose oxidase is a highly specific enzyme (for D-glucose, but see Chapter 8), from the fungi Aspergillus niger and Penicillium, which catalyses the oxidation of
		Glucose oxidase and catalase may be used together when net hydrogen peroxide production is to be avoided.
		A major application of the glucose oxidase/catalase system is in the removal of glucose from egg-white before drying for use in the baking industry.
	www.lsbu.ac.uk /biology/enztech/god.html (235 words)

	Structural and Kinetic Properties of Nonglycosylated Recombinant Penicillium amagasakiense Glucose Oxidase Expressed in ... (Site not responding. Last check: 2007-11-06)
		Mediation of glycosylated and partially-deglycosylated glucose oxidase of Aspergillus niger by a ferrocene-derivatised detergent.
		Reconstitution of flavin-adenine dinucleotide in the apoenzyme of glucose oxidase.
		Expression of the Aspergillus niger glucose oxidase gene in A.
	aem.asm.org /cgi/content/full/64/4/1405 (4242 words)

	Advance in glucose biosensors
		The most common strategies for glucose detection can be partitioned into the following groups: those employing glucose oxidase; those using a dehydrogenase enzyme or those relying on an inorganic catalyst for oxidation of glucose or fluorescence due to the combination of fluorescein and glucose.
		Glucose acid Glucose Oxidase (Re) O2 Another glucose biosensor prepared by the deposition of Iridium and glucose oxidase on glassy carbon transducer was reported by Rodriguez et al (7).
		Glucose oxidases and the mediator vinylferrocene were immobilized within the porous, rigid and organically modified silica network in the composite material.
	www2.hawaii.edu /~wqin/chemistry/papers/glucose.htm (2216 words)

	[No title] (Site not responding. Last check: 2007-11-06)
		Glucose oxidase was rendered inactive by removal of the FAD cofactor.
		To prevent glucose oxidation, which prevents the use of the enzyme as a reversible sensor, we removed the FAD cofactor which is required for the reaction.
		In the presence and absence of glucose the multi-exponential analysis (Table 1) indicates that the decay is dominated by a sub-nanosecond component whose contribution is increased by glucose.
	www.wipo.int /cgi-pct/guest/getbykey5?KEY=01/18237.010315&ELEMENT_SET=DECL (9217 words)

	Merck Vet. Edition - Blood Glucose
		A rapid screen can be performed with a glucose oxidase strip (several types are available) and a drop of either whole blood, serum, or plasma.
		Glucose meters use reflectance photometry to measure the degree of chemical reaction in the reagent strip.
		Many glucose meters are sensitive to variation in technique, and reliability is affected by factors such as improper placement of blood on the reagent pad, timing steps, and sample wipe off.
	www.merckvetmanual.com /mvm/htm/bc/150203.htm (347 words)

	Glucose Oxidase
		Glucose oxidase catalyzes the oxidation of glucose to yield D-glucono-d-lactone and hydrogen peroxide.
		The hydrogen peroxide is quantitated via the peroxidase reaction where oxygen is transferred from hydrogen peroxide to a chromogenic acceptor producing a quinoneimine dye.
		The intensity of the dye color is directly proportional to the concentration of glucose in the serum sample.
	www.catacheminc.com /index_files/page0031.htm (146 words)

	Glucose Meter Linearity
		Glucose is the major energy source for the human body and is derived primarily from the dietary carbohydrates.
		Blood glucose determination is central to the diagnosis and management disorders of carbohydrate metabolism such as diabetes mellitus or hypoglycemia.
		The whole blood glucose test method employs a dry reagent technology based on the glucose oxidase method and is specific for D-glucose.
	path.upmc.edu /showcase/posters/glucline.html (961 words)

	Diabetes - Glucose Oxidase
		Glucose Oxidase: A much used and much loved enzyme in biosensors Introduction to glucose oxidase.
		Glucose oxidase (test enzyme) solution prepared in 0.1 M phosphate buffer, pH 7.0 to yield a final concentration of 0.15 U/ml.
		Occurrence: Glucose oxidase is found in the filamentous fungi, such as, Aspergillus niger and Penicillium notatum.
	www.diabetes-hq.com /GlucoseOxidase (423 words)

	Factors Affecting Glucose Oxidase Activity
		Various factors which affect glucose oxidase activity are demonstrated.
		Glucose oxidase reacts specifically with glucose, only slightly with 2-deoxyglucose, and not with maltose.
		Glucose oxidase is more active at 22°C than at 4°C. Copyright © 2001 Division of Chemical Education, Inc., American Chemical Society.
	jchemed.chem.wisc.edu /JCESoft/CCA/CCA5/MAIN/2BIOCHEM/BIOCHEM2/GLUOXID/MENU.HTM (75 words)

	Urine Glucose Oxidase Test
		Diabetes mellitus (sugar diabetes) is a condition that causes problems in controlling the amount of sugar (glucose) in the blood.
		This lack of insulin results in a low absorption of glucose, both by the body's cells, which need it for energy, and by the liver, which stores it.
		Another result is an abnormally high level of glucose in the blood, which is then excreted in the urine.
	www.wboc.com /global/story.asp?s=1228808 (250 words)

	Invitrogen - Molecular Probes - Figure 10.68 - Detection of glucose oxidase using the Amplex Red Glucose/Glucose ...
		Reactions containing 50 µM Amplex Red reagent, 1 U/mL HRP, 50 mM glucose and the indicated amount of glucose oxidase in 50 mM sodium phosphate buffer, pH 7.4, were incubated for 30 minutes at room temperature.
		Fluorescence was measured with a fluorescence microplate reader using excitation at 530 ± 12.5 nm and fluorescence detection at 590 ± 17.5 nm.
		Background fluorescence (19 units) determined for a no–glucose oxidase control reaction was subtracted from each value.
	probes.invitrogen.com /handbook/figures/0048.html (124 words)

	Glucose oxidase_Mechanism (Site not responding. Last check: 2007-11-06)
		Early investigations into the detailed reaction mechanism of glucox were influenced by the chemical oxidation of glucose by inorganic oxidising agent such as potassium permanganate, sodium hypoiodite and bromine water.
		Although various mechanism have been proposed for the reaction of glucox with glucose, in the author's opinion the one presented below is compatible with the bulk of experimental observation.
		This has been demonstrated by calculating that at an infinite concentration of glucose and a finite concentration of oxygen, an appreciable amount of enzyme still remains in the oxidised form.
	www-biol.paisley.ac.uk /marco/enzyme_electrode/chapter3/chapter3_page7.htm (432 words)

	Immunotargeting of glucose oxidase to endothelium in vivo causes oxidative vascular injury in the lungs -- ...
		Immunotargeting of glucose oxidase to endothelium in vivo causes oxidative vascular injury in the lungs -- Christofidou-Solomidou et al.
		Immunotargeting of glucose oxidase to endothelium in vivo causes oxidative vascular injury in the lungs
		In vivo administration of glucose oxidase conjugated with monoclonal antibodies to angiotensin-converting enzyme.
	ajplung.physiology.org /cgi/content/full/278/4/L794 (5788 words)

	Assay Procedure Glucose Oxidase
		Glucose Oxidase [GO] catalyses the oxidation of Glucose to Gluconic Acid.
		Allow glucose to mutarotate for one hour before using same.
		It should be noted that the Glucose Oxidase assay procedure described above involves the oxygenation of the chromogen solution, o-dianisidine.
	www.faizyme.com /assagluo.htm (422 words)

	Glucose oxidase_Introduction (Site not responding. Last check: 2007-11-06)
		Glucose Oxidase: A much used and much loved enzyme in biosensors
		Glucose oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose and oxygen from beverages and foodstuffs.
		Furthermore, Glucose oxidase-producing moulds such as Aspergillus and Penicillum Species are used for the biological production of gluconic acid.
	www-biol.paisley.ac.uk /marco/enzyme_electrode/chapter3/chapter3_page1.htm (234 words)

	Worthington Glucose Oxidase
		Since its discovery as an "antibiotic" (shown subsequently to be due to peroxide formation) there has been an interest in glucose oxidase, chiefly because of its utility in glucose estimation.
		The purified enzyme is free of these traces and is recommended for analytical use in the presence of di- or polysaccharides.
		Swoboda, B., and Massey, V.: On the Reaction of the Glucose Oxidase from Aspergillus niger with Bisulfite, J Biol Chem 241, 3409, 1966
	www.worthington-biochem.com /GOP (2405 words)

	Diabetes Monitor - meters & management
		hen people with diabetes can control their blood sugar (glucose), they are more likely to stay healthy.
		system is intended for occasional use and to discover trends in glucose levels during the day.
		Walford S et al: Self-monitoring of blood glucose — improvement of diabetic control: Lancet 1: 7320735, 1978.
	www.diabetesmonitor.com /b07.htm (5807 words)

	Potentiometric measurement of glucose concentration with an immobilized glucose oxidase/catalase electrode -- Wingard ...
		Potentiometric measurement of glucose concentration with an immobilized glucose oxidase/catalase electrode -- Wingard et al.
		Potentiometric measurement of glucose concentration with an immobilized glucose oxidase/catalase electrode
		continuous in vivo monitoring of the glucose concentration in body fluids.
	care.diabetesjournals.org /cgi/content/abstract/5/3/199 (194 words)

	Glucose Oxidase Substrates (Site not responding. Last check: 2007-11-06)
		The substrate kits offered for use with the glucose oxidase system provide maximum sensitivity and optimal staining for glucose oxidase.
		The glucose oxidase substrate kits are based on the oxidation of glucose and the reduction of tetrazolium salts to colored, insoluble formazans.
		Each kit is designed to provide approximately 300 ml of working substrate solution which is usually sufficient to stain 1000-3000 tissue sections, and is stable for one year when stored at 4 C. Glucose Oxidase Substrate Kit I (SK-3100)
	www.vectorlabs.com /Substrates/substGO.html (103 words)

	JCE 1999 (76) 1711 [Dec] An Alternative Procedure for the Glucose Oxidase Assay of Glucose as Applied to the Lactase ...
		JCE 1999 (76) 1711 [Dec] An Alternative Procedure for the Glucose Oxidase Assay of Glucose as Applied to the Lactase Activity Assay
		The glucose oxidase assay of glucose has been modified to eliminate the use of micropipets.
		An Alternative Procedure for the Glucose Oxidase Assay of Glucose as Applied to the Lactase Activity Assay J.
	jchemed.chem.wisc.edu /Journal/Issues/1999/Dec/abs1711.html (136 words)

	Immobilized glucose oxidase used in the continuous-flow determination of serum glucose -- Leon et al. 22 (7): 1017 -- ...
		Immobilized glucose oxidase used in the continuous-flow determination of serum glucose -- Leon et al.
		Immobilized glucose oxidase used in the continuous-flow determination of serum glucose
		system and the free glucose oxidase method used in the AutoAnalyzer.
	www.clinchem.org /cgi/content/abstract/22/7/1017 (180 words)

	New Glucose Biosensor Based on Glucose Oxidase-immobilized Gelatin Film Coated Electrodes -- Sungur et al. 18 (4): 265 ...
		New Glucose Biosensor Based on Glucose Oxidase-immobilized Gelatin Film Coated Electrodes -- Sungur et al.
		oxidase enzyme electrode have been studied and results were
		Key Words: glucose biosensor •; amperometric biosensor •; immobilized glucose oxidase • gelatin • cross-linking • chromium III acetate
	jba.sagepub.com /cgi/content/short/18/4/265 (182 words)

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