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| | A Dynamin-Like Protein Associates with the Golgi Apparatus |
 | | Because similar membrane-budding events occur at the Golgi apparatus and multiple dynamin isoforms exist, we have studied the distribution of dynamins in mammalian cells. |
 | | Upon enriching for Golgi membranes, as determined by the increased immunoreactivity for alpha-mannosidase II, beta-COP, rab 6, and the 105 kD form of the polymeric IgA receptor (IgA rec, lower band), there is a concomitant increase in dynamin immunoreactivity, with the greatest enrichment in the stacked Golgi fraction. |
 | | Golgi fractions (Golgi) were isolated from liver using two different established methods, and consisted of either a stacked Golgi fraction (Prep I), or a mixture of Golgi heavy and Golgi light membrane fractions (Prep II; see Materials and Methods). |
| mayoresearch.mayo.edu /mayo/research/mcniven_lab/gogli_dynamin.cfm (965 words) |
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