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Topic: HPLC

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	A Guide to HPLC
		HPLC is a popular method of analysis because it is easy to learn and use and is not limited by the volatility or stability of the sample compound.
		history section illustrates the HPLC's evolution from the 1970's to the 1990's.
		Modern HPLC has many applications including separation, identification, purification, and quantification of various compounds.
	kerouac.pharm.uky.edu /ASRG/HPLC/hplcmytry.html (138 words)

	HPLC - High Performance Liquid Chromatography Primer (Site not responding. Last check: 2007-10-16)
		The name “HPLC” originally referred to the fact that high pressure was needed to generate the flow required for liquid chromatography in packed columns.
		High Performance Liquid Chromatography (HPLC) is now one of the most powerful tools in analytical chemistry, with the ability to separate, identify and quantitate the compounds that are present in any sample that can be dissolved in a liquid.
		HPLC can be applied to just about any sample, such as pharmaceuticals, food, nutraceuticals, cosmetics, environmental matrices, forensic samples, and industrial chemicals.
	www.waters.com /watersdivision/ContentD.asp?watersit=JDRS-5LTGBH (1013 words)

	High performance liquid chromatography - Wikipedia, the free encyclopedia
		HPLC is used to separate components of a mixture by using a variety of chemical interactions between the substance being analyzed (analyte) and the chromatography column.
		In isocratic HPLC the analyte is forced through a column of the stationary phase (usually a tube packed with small round particles with a certain surface chemistry) by pumping a liquid (mobile phase) at high pressure through the column.
		The sample to be analyzed is introduced in a small volume to the stream of mobile phase and is retarded by specific chemical or physical interactions with the stationary phase as it traverses the length of the column.
	en.wikipedia.org /wiki/HPLC (2223 words)

	HP 1050 HPLC Agilent chromatograph
		HPLC is a separating technology used to separate very similar molecules from a mixture and then quantify them accurately.
		HPLC (High Performance Liquid Chromatography) is a mode of chromatography wherein the stationary phase is used in form of fine particles packed in a cylindrical column made up of inert material like stainless steel.
		Hence the HPLC columns are densely packed with the adsorbent to achieve significant degree of resolution.
	www.gmi-inc.com /AnyLab/HPLC.htm (2144 words)

	HPLC Application Notes \| ESA Biosciences, Inc. (Site not responding. Last check: 2007-10-16)
		HPLC is one of the most popular techniques for the separations, analysis and characterization of non-chromophoric, water soluble PEG's.
		HPLC with electrochemical detection (EC) provides superior sensitivity for most of the phenols of interest with the exception of the dinitrophenols which are measured by UV absorbance.
		This isocratic HPLC method using fluorescence detection at 553 nm permits the measurement of MDA in tissue.
	www.esainc.com /applications/application_notes.htm (3123 words)

	HPLC portal
		HPLC portal - a categorized directory of links, news, education, reference, training, HPLC columns and equipment
		Used and new equipment, HPLC columns and supplies
		HPLC glossaries, periodic tables, physico-chemical properties, problem solutions
	hplcweb.com (51 words)

	HPLC
		More sophistocated HPLC's may involve more than one pump to generate mobile phases mixtures, robotic arms capable of injecting perhaps 100 samples unattended (autosamplers), small ovens to control column temperature, mass spectral detectors capable of identifying components as they exit the column, and complete computer contral for automation.
		The ability to choose among a wide array of stationary phase allows one to apply HPLC to an enormous variety of complex mixtures from blood serum to hot sauce.
		HPLC is one of the most widely applied analytical separation techniques.
	faculty.kutztown.edu /betts/html/HPLC.htm (720 words)

	HPLC Autosampler (Site not responding. Last check: 2007-10-16)
		The HT300L HPLC autosampler is equipped with an unprecedented number of benefits.
		The tray of the autosampler may be configured in the right or left position, with either a 10-position fixed tray (HT300L-10) or with a 110-position movable tray (HT300L) for vials of 2 and 2.5 ml.
		HPLC large volume autosampler with 40 position tray for 10ml vials.
	www.overbrookscientific.com /template/3_17_12.html (322 words)

	Upchurch Scientific - HPLC Introduction
		High Performance Liquid Chromatography (HPLC) is an analytical process utilizing special instruments designed to separate, quantify and analyze components of a chemical mixture.
		Samples of interest are introduced to a solvent flow path; carried through a column packed with specialized materials for component separation; and component data is obtained through the combination of a detection mechanism coupled with a data recording system.
		The basic components of an HPLC system include a solvent reservoir, pump, injector, analytical column, detector, recorder and waste reservoir.
	www.upchurch.com /TechInfo/hplcIntro.asp (561 words)

	Operation of Waters HPLC
		HPLC can be used for analysis of organic or polymeric samples, so long as the samples are dissolvable in the eluent used for the delivery.
		The sample solution should be prepared with the solvent same as the eluent used in the elution.
		Too high pressure of the HPLC indicates a blocked guard column.
	bridge.ecn.purdue.edu /~changhe/Envhyd/handbook/HPLC_WATERS.html (800 words)

	Wiley::HPLC for Pharmaceutical Scientists
		HPLC for Pharmaceutical Scientists is an excellent book for both novice and experienced pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry.
		It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry.
		In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation.
	www.wiley.com /WileyCDA/WileyTitle/productCd-0471681628.html (224 words)

	Princeton Chromatography HPLC Columns and Accessories
		The 60Å, 400 m/g spherical silica used for the stationary phase support was selected to give sufficient capacity for complex mixtures analyzed in the gradient elution mode.
		PrincetonSPHER-PFP HPLC columns are useful for differentiating Halogen bearing compounds from their non-Halogened analogs.
		PrincetonSPHER-Fluorooctyl HPLC columns are useful for differentiating Halogen bearing compounds from their non-Halogened analogs.
	www.pci-hplc.com /Pages/HPLCColumns.htm (934 words)

	HPLC - Prominence HPLC - Shimadzu North America
		HPLC systems are currently used in a wide variety of fields.
		The world's first HPLC control by a web browser is available with the CBM-20A.
		Connection of HPLCs and PC workstations via the Ethernet enhances flexibility of instrument installation to efficiently use laboratory space, and provides a flexible analytical network environment by controlling access to the configured HPLC systems from client computers.
	www.ssi.shimadzu.com /products/product.cfm?product=prominence (532 words)

	HPLC Reagents
		Pre- and post-column derivation/detection reagents for HPLC analysis of peptides and proteins.
		Pierce HPLC solvents such as trifluoroacetic acid (TFA), formic acid and heptafluorobutyric acid (HFBA) are purified and tested to the highest standards, then packaged in amber glass and teflon containers to ensure quality and consistency of performance.
		HPLC standards are an indispensible tool for analytical HPLC applications.
	www.piercenet.com /Products/Browse.cfm?fldID=030109 (117 words)

	AMD (Site not responding. Last check: 2007-10-16)
		He is the co-author of Troubleshooting HPLC Systems, which has become a standard reference work in this area.
		While dr. Dolan continues to be involved in the design of HPLC method development software, his primary role as General Manager of BASi Northwest Laboratory (Bioanalytical Systems, Inc.) provides him with a unique "hands-on" perspective on the challenges of chromatography in pharmaceutical and biotech applications.
		He is a member of the Editorial Advisory Board of LC-GC and also teaches the Practical HPLC Method Development course for the American Chemical Society.
	www.hplc.hu /amd.htm (133 words)

	W.M. Keck Facility at Yale - Peptide HPLC
		Automated peptide mapping by C-18 reverse-phase HPLC is available on an HP1090 system that has been optimized for high sensitivity HPLC.
		Digests are loaded in 50 µl of a suitable solvent, (which should not contain detergent) and should contain from 5 to 100 pmol protein.
		Protein digests that contain from 5 to 100 picomoles in a volume of 50 µl may be fractionated by C-18 reverse-phase HPLC and automatically collected with peak detection into 100-150 individual Eppendorf tubes.
	info.med.yale.edu /wmkeck/prochem/hplc.htm (168 words)

	Sonntek HPLC Instruments
		Well plates are economic in use, available in a variety of materials and sizes, can be sealed, and fit in a growing number of instruments such as the 96 well SPE manifolds.
		But, well plates were not designed for HPLC autosampling and HPLC autosamplers were not prepared for today's variety of well plates.
		You are operating HPLC systems and you need to increase sample throughput, you must comply with GMP/GLP directives, and you want a substantial cost reduction.
	www.sonntek.com /hplc/autosamplers.html (648 words)

	Chromachem(R) Evaporative Light Scattering Detector (ELSD) for HPLC \| New from ESA Inc (Site not responding. Last check: 2007-10-16)
		Detection from Evaporative Light Scattering is based on the universal ability of particles to cause photon scattering when they traverse the path of a polychromatic beam of light.
		The liquid effluent from an HPLC is first nebulized and the resultant aerosol mist, containing the analyte particles, is directed through a light beam.
		A signal is generated that is proportional to the mass present, and independent of the presence or absence of chromophores, fluorophores or electroactive groups.
	www.esainc.com /products/HPLC/Optical_detectors/esa_ChromaChem.html (402 words)

	Surveyor Plus HPLC System-Thermo Scientific
		The Surveyor Plus family of HPLC instruments is specifically optimized for the most demanding HPLC and LC/MS applications.
		Method development using the Surveyor Plus HPLC system is easily optimized because of its multiple injection modes, which include full loop, partial loop, and no waste.
		HPLC Analysis of Protease Inhibitors Used in the Treatment of HIV
	www.thermo.com /com/cda/product/detail/1,,10121464,00.html (328 words)

	Reversed Phase Chromatography HPLC columns - Amberchrom Profile (Site not responding. Last check: 2007-10-16)
		AMBERCHROM™ Profile™ Reversed Phase Chromatography HPLC columns are an extension of our existing AMBERCHROM™ XT line offering 10, 20, and 30 µm particle sizes with higher pressure capabilities, improved resolution and the same exceptional stability to caustic CIP procedures.
		AMBERCHROM™ Profile™ HPLC columns are targeted for peptide, protein, and oligonucleotide purification and the methods development needs of the biopharmaceutical industry.
		AMBERCHROM™ Profile™ HPLC columns provide customers with an easy, ready to use format for their resin evaluation and scale up requirements in purifying hydrophobic and polar peptides, small proteins and oligonucleotides.
	www.rohmhaas.com /ionexchange/Pharmaceuticals/amberchrom_profile.htm (357 words)

	HPLC-GC
		The innovative KONIK-TECH patented TOTAD interface marries in synergy the separation and fractionation potential of LC and HPLC, to the separation, selective detection and quantitation of HRGC, opening new dimensions...
		The HPLC column and solvents used can contribute totally or partially to the sample clean up depending on the complexity of the matrix.
		Both HPLC and LC methods using reversed phase (organic phases and polar solvents) as well as direct phase methods (columns with silica, alumina and the like and apolar solvents) are equally suitable to the System.
	www.konik-group.com /hplcgc.htm (1089 words)

	HPLC
		For example, if the partitioning partition coefficient for A is 0.1 and the concentration of stationary sites is 10 equiv/L, then divide the partition coefficient by 10 equiv/L to get the competitive partition coefficient, P(A)=0.01.
		For a faster and more complete version of this program try: Programmed HPLC Simulator.
		Introduction to HPLC Simulator discusses the theory and applications of the program.
	www.colby.edu /chemistry/NMR/hplc.html (162 words)

	Welcome to HPLC Institute
		HPLC Analysis and Purification of Proteins and Peptides
		The need to isolate proteins and peptides in sufficient quantities to identify activity and determine structure, as well as to analyze for purity, has placed heavy demands on laboratory personnel to become proficient in the use of this technique.
		Those just beginning in HPLC will gain a solid understanding of the basics, and will obtain a much greater grasp of how to use the technology to operate successfully with a minimum of downtime.
	www.hplcinstitute.com /hplc_schedule/proteins_schedule.php (395 words)

	HPLC Systems - Used Surplus Equipment
		HP 1050 HPLC System with UV/Vis Detector, including: HP 79852A quaternary pump; HP 79856A solvent tray with column heater; HP 79855A autosampler(21 positions); HP 7...
		We are looking for a complete HPLC system of a renowned company for our Pharmaceutical Factory.
		This HPLC system was recently retired from a working lab.
	www.labx.com /v2/newad.cfm?CatID=3 (794 words)

	HPLC
		► An HPLC instrument has lots of parts, and each one does a special job.
		Solvent Bottle The HPLC uses a liquid to push the sample.
		Detector Once we separate the molecules, we have to figure out which ones came through the column at what time.
	www.chemistry.ohio-state.edu /~pmazzer/HPLC.htm (1010 words)

	DONtest HPLC Mycotoxin Testing System - Mycotoxin Products - VICAM
		Deoxynivalenol (DON) is also known as vomitoxin because of its impact on livestock through interference with animal growth and acceptance of feed.
		In addition to its speed, sensitivity and accuracy, DONtest HPLC is easy to use.
		VICAM’s undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness for a particular purpose.
	www.vicam.com /products/dontest-hplc.html (458 words)

	HPLC - High Performance Liquid Chromatography from PerkinElmer - USA (Site not responding. Last check: 2007-10-16)
		PerkinElmer offers a full range of HPLC solutions that meet even the most demanding requirements.
		With over 30 years of LC experience going into every one of our products - you can count on outstanding performance and reliability â€“ backed by our renowned global support network for total piece-of-mind.
		Automate your HPLC method development and robustness testing requirements.
	las.perkinelmer.com /Catalog/default.htm?CategoryID=HPLC (111 words)

	JM Science: HPLC instruments HPLC equipment Hitachi HPLC Columns Asahipak Shodex Cosmosil CapcellPak HPLC columns >> ...
		These new HPLC columns are based on a highly cross-linked, porous, monolithic methacrylate polymer that delivers ultra-fast separation of macromolecules with high resolution and binding capacity.
		Preparative CIM (Convective Interaction Media) HPLC columns are designed for very fast preparative separations and purification of biomolecules from complex mixtures as well as for various biotransformations.
		CIM HPLC columns are available in different chemistries and may be used in the high performance anion-exchange, cation-exchange, hydrophobic interaction and affinity chromatography modes.
	www.jmscience.com /hplc-columns.php (1128 words)

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