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Topic: Haemolysis

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	[No title]
		Haemolysis is the release of intracellular components from erythrocytes, thrombocytes and leukocytes into the extracellular fluid, i.e.
		Haemolysis is visible as a red colouration of the plasma or serum after centrifugation of the sample.
		Even if in vitro haemolysis occurs more frequent than in vivo, the latter is of greater clinical importance because of its pathological origin, and because the parameters influenced by haemolysis are relevant for diagnosis, follow-up and therapeutic monitoring of the diseases.
	www.ifcc.org /ejifcc/vol13no4/130401002eng.htm (1903 words)

	Naphthalene (PIM 363)
		Haemolysis is more likely to occur in individuals with a hereditary deficiency of glucose-6-phosphate dehydrogenase, sickle cell anaemia and sickle cell trait.
		Acute intravascular haemolysis is the most characteristic sign, particularly in persons with red cell glucose-6-phosphate dehydrogenase deficiency; it is accompanied by anaemia, leucocytosis, fever, haemoglobinuria, jaundice, renal insufficiency and sometimes disturbances in liver function.
		Haemolysis mostly occurs in individuals with a hereditary deficiency of glucose-6-phosphate dehydrogenase due to instability of erythrocyte glutathion.
	www.inchem.org /documents/pims/chemical/pim363.htm (4171 words)

	Haemolysis Artefact on blood Tests (Site not responding. Last check: 2007-10-22)
		Haemolysis, simply defined, is the process that leads to lysis (or rupture) of the RBC wall, causing the RBC to disintegrate and release its internal contents into the plasma, or fluid part of the blood.
		The degree or severity of haemolysis is reflected in the intensity of the red colour of the plasma.
		The effects of haemolysis on patients test results is somewhat test dependent and varies greatly with the degree of haemolysis in the specimen.
	www.gpl.net.au /Haemo.htm (660 words)

	Haemolysis following implantation of duct occlusion coils -- Uzun et al. 81 (2): 160 -- Heart
		Haemolysis was detected in five of 137 procedures following implantation of Cook detachable duct coils.
		Haemolysis is an important complication after duct coil implantation.
		Haemolysis occurred in five children (two girls, three boys) with arterial ducts and otherwise normal hearts after coil implantation.
	www.heartjnl.com /cgi/content/full/81/2/160 (1317 words)

	Haemolysis and Lipaemia (Site not responding. Last check: 2007-10-22)
		Haemolysis is a condition that occurs when haemoglobin is released from red blood cells when the cell membranes are damaged.
		The fact that haemolysis has occurred may not be apparent until the separation of the serum or plasma has taken place.
		Haemolysis will affect certain biochemical values when a colorimetric photometric method is used to determine these values.
	www.goldenvetlab.co.za /haemolysis_and_lipaemia.htm (502 words)

	Single Radial Haemolysis, SRH
		Single radial haemolysis (SRH) is routinely used for the detection of rubella-specific IgG.
		The size of the zone of haemolysis around a well containing test serum is compared to that of the 15 IU/ml control.
		Hazy zones of haemolysis may also occur when acute phase sera are tested due to the presence of low avidity antibodies.
	virology-online.com /general/Test6.htm (526 words)

	[No title]
		Haemolysis may be intravascular and extravascular or a combination of both.
		Following substance-induced haemolysis, haemosiderin deposition can be accelerated to such levels that the accumulated pigment exceeds the normal amount of deposits in the spleen and may affect other organs as well.
		During prolonged haemolysis, an increase in haemosiderin accumulation is expected to be seen in the bone marrow.
	ecb.jrc.it /classlab/0703a11_NL_haemolytic_anaemia_finalreport.doc (5991 words)

	Effects of various arterial infusion solutions on red blood cells in the newborn -- Jackson and Derleth 83 (2): 130 -- ...
		The mean haemolysis and standard error for each solution, based on the total number of samples used, are statistically compared by a two sample t test.
		Percentage haemolysis is less for the NS group (p < 0.001) than for all for the other groups, apart from the isotonic sodium acetate group (p = 0.466).
		Haemolysis was significantly higher with 0.25% NS than with all the other solutions tested (all p < 0.004; table 3).
	fn.bmjjournals.com /cgi/content/full/83/2/F130 (3331 words)

	Appendices 1-18 to: Report on the Health Effects of Selected Pesticide Coformulants
		Haemolysis and haemoglobinuria are common observations in animals treated orally with EGBE for acute duration (ATSDR 1998).
		Male rats receiving a single dose of 500 mg/kg by gavage exhibited haemolysis of erythrocytes accompanied by a drastic increase in the concentration of free haemoglobin in the plasma and haemoglobinuria was observed in all rats.
		In male rats given 250 mg/kg by gavage, haemolysis was noted, characterised by an increase in mean cell volume and haematocrit, followed by a decline in haemoglobin concentration and RBC count.
	www.mst.dk /udgiv/publications/2003/87-7614-055-5/html/kap81_eng.htm (6651 words)

	Entrez-PubMed (Site not responding. Last check: 2007-10-22)
		Haemolysis in haemodialysis patients: evidence for impaired defence mechanisms against oxidative stress.
		This led to haemolysis in 24 patients, while nine were unaffected.
		There was no correlation between the enzyme levels and the degree of haemolysis.
	www.geocities.com /mboaz8/Tali.htm (317 words)

	Nationwide Laboratories - Test Interpretation page 1
		Haemolysis, lipaemia and icterus in a serum or plasma sample may lead to false elevations (or reductions) in the measured concentrations of biochemical analytes.
		The presence of haemolysis or lipaemia is indicated on the report and affected analytes are flagged.
		Usually there is a false elevation in measured concentration, the exceptions include glucose (reduced due to haemolysis), calcium (reduced due to gross haemolysis) and creatinine (occasionally reduced due to haemolysis).
	www.nwlabs.co.uk /testinterp1.htm (597 words)

	Effects of ageing and increased haemolysis on the levels of dolichol in rat spleen. (Site not responding. Last check: 2007-10-22)
		Effects of ageing and increased haemolysis on the levels of dolichol in rat spleen.
		The effects of haemolysis on spleen dolichol were tested by the administration of phenylhydrazine.
		It is concluded that the levels of spleen dolichol may decrease in the absence of any known enzymatic degradative pathway if the spleen and its resident phagocytes are forced to cope with a higher number of red blood cells to be cleared.
	www.arclab.org /medlineupdates/abstract_11738151.html (274 words)

	North Western Laboratories Newsletter
		Haemolysis can be defined as damage to, or destruction of, red blood cells resulting in leakage of haemoglobin and red discolouration of the serum/plasma.
		There are many causes of haemolysis, it can occur as a pathological event, as in the case of immune mediated haemolytic anaemias, however, the majority of haemolysis occurs either during or after blood collection.
		As previously mentioned, haemolysis imparts a red colour to serum/plasma, the intensity of which is proportional to the degree of haemoglobin released, and this will interfere with photometric methods of biochemical analysis.
	www.nwlabs.co.uk /newsletter61.htm (1780 words)

	g6pd : significance : favism
		Haemolysis in breast-fed babies whose mothers have ingested fava beans have also been reported.
		Although all persons with favism are glucose-6-phosphate dehydrogenase deficient, not every glucose-6-phosphate dehydrogenase deficient subject develops haemolysis after ingestion of fava beans.
		This standing was illustrated in a study done on 102 patients with no previous history of drug induced haemolysis who have been consuming fava beans on a regular basis without developing any ill effects up to the point of the haemolytic episode.
	meltingpot.fortunecity.com /nepal/671/g6pdfavism.html (417 words)

	Comparison of p25 presequence peptide and melittin. Red blood cell haemolysis and band 3 aggregation. (Site not responding. Last check: 2007-10-22)
		Red blood cell haemolysis and band 3 aggregation.Aggregation cannot be reversed by high ionic strength; this serves to differentiate these peptides from other positively charged species such as polylysine that aggregate band 3 at low ionic strength.
		The present study extends the results of leakage experiments on model-membrane systems to the haemolysis of human erythrocytes, which both peptides are shown to accomplish in a manner sensitive to membrane potential.
		The 25 residue presequence (p25) for subunit IV of yeast cytochrome oxidase had previously been shown to possess structural and behavioural characteristics in common with the bee venom polypeptide, melittin.
	www.pdg.cnb.uam.es /UniPub/iHOP/gp/6081770.html (164 words)

	Appendices 1-18 to: Report on the Health Effects of Selected Pesticide Coformulants
		The possibility that certain human subpopulations, including the aged and those predisposed to haemolytic disorders, might be at an increased risk from exposure to EGBE has been investigated using blood from the elderly, from patients with sickle cell disease, and from patients with spherocytosis.
		These effects, which generally occur at higher exposure levels than those causing haemolysis, are secondary to the haematotoxicity of EGBE and a result of a compensatory response to the haemolysis.
		Data indicate that humans are less sensitive to the haemolytic toxicity of EGBE than are rats as no or very slight haemolytic effects were observed in the poisoning cases after acute oral ingestion of large doses.
	www.mst.dk /udgiv/publications/2003/87-7614-055-5/html/kap84_eng.htm (1621 words)

	Acta Cardiologica - Abstract, vol.58 - nr. 5/1 - 2003 (Site not responding. Last check: 2007-10-22)
		Laboratory tests for evidence of haemolysis namely, haemoglobin (Hb), haematocrit (Hct), plasma total bilirubin, indirect bilirubin and haptoglobin levels, corrected reticulocyte percent and serum lactic dehydrogenase activity (SLDH) were performed before and after the PTX treatment.
		Additionally, there were significant decreases in SLDH, total and indirect bilirubin levels, and corrected reticulocyte percent in patients receiving PTX as compared with their respective control values (p < 0.01, for all).
		PTX treatment caused a significant improvement, to different extents, in signs of haemolysis in 60% of the patients.
	www.actacardiologica.be /abstracts/abs5805200301.htm (254 words)

	Plasma vitamins A and E and hydrogen peroxide-induced in vitro erythrocyte haemolysis in common marmosets (Callithrix ...
		Plasma vitamins A and E and hydrogen peroxide-induced in vitro erythrocyte haemolysis in common marmosets (Callithrix jacchus) -- Ghebremeskel et al.
		Plasma total lipid, cholesterol, all-trans retinol (vitamin A) and alpha-tocopherol (vitamin E) concentrations, and the susceptibility of erythrocytes to hydrogen peroxide-induced haemolysis in vitro were investigated in healthy laboratory-bred common marmosets (Callithrix jacchus).
		The susceptibility of the erythrocytes of the marmosets to hydrogen peroxide-induced haemolysis was high, both in absolute value and relative to the controls.
	veterinaryrecord.bvapublications.com /cgi/content/abstract/126/17/429 (172 words)

	AcademicDB - Complement Practical
		From the graph(s), it can be seen that the amount of haemolysis occurring in each of the three tubes does vary.
		Tube 2 shows more haemolysis than in tube 1 as it occurred in the first three wells, but the amount of haemolysis steadily decreased with dilution until by the fourth, there was none.
		Tube 3 showed the greatest amount of haemolysis as complete haemolysis occurred in three of the twelve wells and partial haemolysis in a further three wells.
	www.academicdb.com /complement_practical_4372 (366 words)

	Massive acute haemolysis in neonates with glucose-6-phosphate dehydrogenase deficiency -- Dhillon et al. 88 (6): 534 -- ...
		Massive acute haemolysis in neonates with glucose-6-phosphate dehydrogenase deficiency -- Dhillon et al.
		Massive acute haemolysis in neonates with glucose-6-phosphate dehydrogenase deficiency
		Contribution of haemolysis to jaundice in Sephardic Jewish glucose-6-phosphate dehydrogenase deficient neonates.
	fn.bmjjournals.com /cgi/content/full/88/6/F534 (1768 words)

	Streptococci, Streptococcus Pyogenes Infection
		This is due to the release of streptolysin O and S. a-haemolytic streptococcus are surrounded by a narrower zone of haemolysis.
		This classification of streptococci based on haemolysis is not entirely reliable, because there is considerable strain variation e.g.
		Streptococci are readily cultured from blood agar plates where a or B haemolysis may be seen.
	virology-online.com /Bacteria/Streptococci.htm (668 words)

	Indian Journal of Medical Research: Report of unusual clinical appearance in bacteraemia with non-haemolytic M-type 58 ...
		Results: In the first case, of a 75 year-old man with leukaemia and a retropharyngeal abscess, the isolate was consistently non-haemolytic, probably due to a failure to produce streptolysin S as has been described before in a small number of reports involving various M-types.
		In the first patient there was a very narrow zone of greening around the colonies but no haemolysis in either aerobic or anaerobic cultures; failure to lyse red biood cells around the colonies in any part of the culture plate was confirmed by low power light microscopy.
		However, zones of haemolysis were smaller where the colonies were more widely separated and, in the most thinly spread parts of the plate, the colonies showed small haloes of greening but no haemolysis (confirmed by microscopy), resembling those of the first patient.
	www.findarticles.com /p/articles/mi_qa3867/is_200405/ai_n9451247 (1440 words)

	[No title]
		Extracted saponin from the seed was used for erythrocyte fragility tests (haemolysis) while buffered saline extract of the seed meal was used for toxicity test with 3-week old broiler chicks.
		The samples were mixed thoroughly with heparin and were viewed under the microscope for haemolysis as in the case of human blood.
		In this investigation, there was no observed haemolysis in the blood of all the chicks (in the bioassay test) with a microscope and this corroborates the ascertion of Trease and Evans (1983).
	www.cipav.org.co /lrrd/lrrd15/12/ukpa1512.htm (2286 words)

	[No title] (Site not responding. Last check: 2007-10-22)
		Haemolysis associated with continuous venovenous renal replacement circuits
		High pressures and clots within the circuit have been identified as factors increasing the likelihood of haemolysis.
		PFHb was measured during CVVHD to determine if circuit life, maximum circuit pressure or the clotting of the haemofilter was associated with evidence of haemolysis.
	www.aaic.net.au /TOC.asp?View=2&A=3004 (128 words)

	5.2 Haemolytic Transfusion Reactions (Site not responding. Last check: 2007-10-22)
		Acute intravascular haemolysis may cause an acute cardiovascular collapse with hypotension, disseminated intravascular coagulation and acute renal failure.
		The important differentiating point is the absence of haemolysis in the septic patients.
		Look for evidence of haemolysis: inspection of the urine if the patient has an IDC is the best method.
	www.qldanaesthesia.com /TransfnBook/Trans5_2.htm (826 words)

	Haemolysis
		Haemolysis may be caused by bacterial haemolysins, by antibodies that cause complement-dependent lysis, by placing red cells in a hyptonic solution, or by defects in the red cell membrane.
		"Haemolysis" is generally used as a noun (singular) -- approximately 100.00% of the time.
		"Haemolysis" is used about 15 times out of a sample of 100 million words spoken or written in English.
	www.websters-online-dictionary.org /definition/english/Ha/Haemolysis.html (386 words)

	WCH Clinical Toxinology Resources
		However, clinically significant haemolysis is not common after bites by most species.
		If haemolysis occurs at all, it is usually only minor, insufficient to cause either significant anaemia or secondary renal failure.
		There is true haemolysis, but it is not caused by direct venom damage to the red cells.
	www.toxinology.com /fusebox.cfm?staticaction=snakes/ns-clinic101.htm (305 words)

	[No title]
		It should be noted that the standard requirements for 28 day studies does not include a number of testing parameters that may give valuable information on the haemolytic anaemia, e.g., it is not required to test for urinalysis, unconjugated or even total bilirubin, or red cell morphology.
		This effect is seen after a delay of a few days after the start of the haemolysis (B) and may persist for several days after haemolysis has terminated.
		In case of extravascular haemolysis, haemosiderin accumulation is expected to be seen in the bone marrow.
	ecb.jrc.it /classlab/0703a6r3_NL_haemolytic_anaemia.doc (3569 words)

	British Journal of Biomedical Science: Bimodal role for divalent cations in the mechanism of EDTA cytolysis
		The roles of both Ca 2+ and Mg+on haemolysis were studied further at a fixed EDTA concentration (10 mmol/L).
		Calcium cations produced a dose-dependent increase in EDTA-induced haemolysis, up to a maximal level of 5 mmol/L. Thereafter, it produced a progressive reduction in haemolysis, in a dose-dependent manner, gradually approaching that of the control buffer level (Figure 1).
		A practical application of this study would include the common observation of haemolysis, as shown by dark brown discolouration of plasma, when high levels of EDTA are used either as anticoagulant or enzyme inhibitor in blood samples.
	www.findarticles.com /p/articles/mi_qa3874/is_200001/ai_n8888841 (1251 words)

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