
	Where results make sense

Topic: Hematoxylin

Related Topics

Histology

Mordant

Sucralose

Suedela

Curelom

Fentanyl

Patronus

Aspartame

Saccharin

Sugar substitute

Nanoshell

Jebel Marra

Ralph Izard

Sweetener

List of organic compounds

	Stainsfile - Hematoxylin staining - introduction
		Hematoxylin (C.I.) is a natural dye extracted by boiling the wood of the South American and West Indian logwood tree (Haematoxylon campechianum), and partly purified by recrystallization.
		Remember that the original hematoxylin was a crude extract of logwood, so it may contain hundreds of unknown substances besides hematoxylin, and some of them and their oxidized products may be responsible for some of the precipitates.
		The principles just outlined for hematoxylin staining also apply to eosin Y or the other red counterstains, except that the endpoint is not quite as sharp.
	stainsfile.info /StainsFile/stain/hematoxylin/hxintro.htm (1868 words)

	Stainsfile - Hematoxylin
		Hematoxylin is a natural dye which is extracted from the heartwood of the tree Haematoxylum campechianum, although histotechnologists are probably more familiar with the name as Hematoxylon campechianum.
		Although it is common practice to use hematoxylin, it is not itself the dye.
		The dye is usually used in conjunction with a mordant, the two commonest being aluminum (as ammonium or potassium alum), or iron (ferric chloride or iron alum).
	stainsfile.info /StainsFile/dyes/75290.htm (530 words)

	Our debt to the logwood tree: the history of hematoxylin Medical Laboratory Observer - Find Articles
		Often used to highlight nuclear detail, hematoxylin has been described as "the cornerstone for staining tissue sections" and as the "foundation upon which modern histopathological diagnosis is built." (1) It is a stain derived from a natural source.
		Hematoxylin is a natural dye derived from the Haematoxylum campechianum tree, (2) a medium-sized, evergreen, thorny tree with small, five-petal, yellow malodorous flowers.
		(6) Crude hematoxylin is extracted from chips of the heartwood using hot water or steam, and has been used as an astringent to treat diarrhea and dysentery, (4) as a pH indicator, and as dye for clothing or tissue.
	www.findarticles.com /p/articles/mi_m3230/is_5_38/ai_n16419252 (1122 words)

	Virtual Lab
		Hematoxylin, a basic dye, binds to acidic components of a tissue, which are thus said to be "basophilic." The color of the stained structures depends on the mordant used to make the hematoxylin dye bind to the molecules of the tissue.
		Hematoxylin, when combined with iron as a mordant, can be used alone to bring out structures not clearly delineated otherwise, including mitochondria, muscle fiber striations and the myelin sheaths of nerves.
		This section is stained with a combination of iron hematoxylin (stains chromatin and cytoplasmic ribosomes fl to brown) and aniline blue, which differentiates the connective tissue septa of this gland.
	casweb.cas.ou.edu /pbell/Histology/Outline/cell.html (1354 words)

	[No title]
		Hematoxylin is extracted from logwood, otherwise known as campeachy wood.
		Hematoxylin used for the Pap stain is typically one of the Gill’s hematoxylins (discussed in the HandE section) but Harris Hematoxylin is also used.
		Gill’s Hematoxylin uses ethylene glycol as the solvent for the hematoxylin in order to prevent the formation of surface precipitants and assist with diffusion and penetration.
	www.statlab.com /Protocol/CytologyStaining.htm (1112 words)

	Counterstains (Site not responding. Last check: 2007-08-08)
		This hematoxylin may be applied to tissues immuno-stained with alcohol-soluble enzyme reaction products, such as AEC, or for providing superb contrast with substrates such as DAB.
		Hematoxylin QS contains no mercury, is ready -to-use without filtration, and provides excellent color contrast with most commonly-used peroxidase and alkaline phosphatase substrates.
		This superior formulation of methyl green is suitable for use with a wide range of enzyme precipitates and is regarded as an ideal alternative to hematoxylin.
	www.vectorlabs.com /cstain/cstain.html (353 words)

	Identification of Cryptosporidium spp. oocysts in fecal smears stained with Heidenhain's iron hematoxylin Revista do ...
		The rather infrequent use of iron hematoxylin stains at present, in parasitological laboratories, could be attributed to some mostly unnecessary complexities frequently included in the prescriptions for the use of this essentially simple, inexpensive and reliable technique2.
		Preparations stained with iron hematoxylin, intended for the identification of trophozoites and cysts of various species of protozoa, must be processed while wet, and require the use of a mounting medium before examination under a high-power immersion objective.
		As further oxidising of hematein produces a substance that is not a stain, a too large amount of an oxidising agent or storage for long periods destroys the staining properties of hematoxylin solutions.
	www.findarticles.com /p/articles/mi_qa3855/is_200111/ai_n9014694 (691 words)

	Engscientific Blood Bank Page
		The hematoxylin stains the WBC nuclei to prevent misinterpretation as eosin-positive cells.
		In the new and old Kleihauer procedure, WBC’s are counterstained with hematoxylin to avoid misidentification as eosin-positive cells.
		The hematoxylin stains the nuclei of the WBC’s blue to prevent misinterpretation as eosin-positive cells.
	www.engscientific.com /blood.html (429 words)

	Hematoxylin and Eosin Staining Problems and Solutions (Site not responding. Last check: 2007-08-08)
		Solution: Small, clear crystals often seen throughout sections may be crystals of aluminum potassium sulfate which is used as a mordant in many hematoxylin solution was not mixed properly resulting in the aluminum potassium sulfate reconverting to its original crystalline form.
		This pigment is identified as hematein (the coloring agent of hematoxylin) which precipitates due to the absence of the mordant described above.
		Using a magnetic stirrer at high speed, dissolve 5 gm of hematoxylin in 75 ml of 70% ethanol.
	www.wv-hsta.org /Nanatomy/Step7.htm (434 words)

	Staining
		Mayor's hematoxylin is used because it eliminates the necessity for differentiation and bluing of the section.
		Because of the simplicity of the technique, it is possible to teach others to use it within a shorter time as well as a definite reduction in time performance of the stain itself.
		Hematoxylin, 5% in 95% alcohol (may be kept as a stock solution).....
	mammary.nih.gov /atlas/histology/jaxworkshop/syllabus/stains.htm (1953 words)

	GTX73341-Aqueous Hematoxylin-GeneTex Inc.
		This hematoxylin provides a clean, strong and permanent nuclear stain in the formalin-fixed, paraffin-embedded tissue sections, as well as in frozen tissue sections.
		Aqueous hematoxylin was designed to overcome one of the major obstacles in immunocytochemistry: the use of alcohol-based hematoxylins which destroy many of the most useful chromogens used for both immunoperoxidase and immunoalkaline phosphatase assays.
		Biømeda¿s hematoxylin provides a clean, strong, superior nuclear stain in only one minute which is 8-10 times faster than other water-based hematoxylins such as Mayer¿s formulation.
	www.genetex.com /ccp27644-aqueous-hematoxylin-m10-gtx73341.htm (303 words)

	Hematox
		Used to rapidly ripen the working hematoxylin staining solution for immediate use when no fully ripened stock solution is available.
		Hematoxylin Bluing: Blue the preparation by washing for 5 minutes in saturated lithium carbonate solution.
		Harris Hematoxylin and Eosin is much more predictable and should be used by inexperienced workers.
	science.peru.edu /gregarina/html/heidhem.html (704 words)

	MFA - CAMEO - Material Description
		Hematoxylin oxidizes to form the reddish-brown metallic crystals of hematein that are insoluble in water.
		Using a variety of mordants, the extract can be prepared as a lake ranging in color from red to brown to navy (chrome), violet (alum) to fl (iron).
		Hematoxylin is also used as a histological stain in the preparation of organic tissues for microscopic examination.
	mfa.org /_cameo/frontend/material_description.asp?name=hematoxylin&... (171 words)

	BBC Biochemical : Products
		The concentration of blueing reagent is formulated to produce the necessary basophilic change of hematoxylin and minimize loss of adherence of tissue sections to glass slides.
		Gill's Hematoxylin II BBC presents Gill's Hematoxylins for nuclear staining of the highest precision and clarity.
		This hematoxylin is designed for specific nuclear staining of the highest precision and clarity using veterinary tissues.
	www.bbcus.com /products.html?pc=27&pid=92 (584 words)

	Hematoxylin-Eosin (Site not responding. Last check: 2007-08-08)
		Hematoxylin and eosin staining is one of the most common method used in histology and anatomy.
		Basophilic nuclei, bacteria, calcium, and so on are stained "blue" with hematoxylin.
		Hematoxylin and eosin staining is often used to detect inflammation or to determine the integrity of a tissue.
	pharmaness.com /hematoxylin-eosin.html (58 words)

	[No title]
		Hematoxylin is a natural dye that is extracted from the heartwood of the logwood tree Haemotoxylon.
		Hematoxylin also stains myelin weakly but the effects is not noticeable if it is combined with eosin stain.
		Since hematoxylin turns blue in alkaline environment, ammonia water or other alkaline solutions such as lithium carbonate are often used for this purpose.
	moon.ouhsc.edu /kfung/JTY1/NeuroHelp/ZNEWBS12.htm (2656 words)

	Richard-Allan Scientific : RAS Signature Series Products
		Hematoxylin 2 is designed to produce staining intensity similar to Harris Hematoxylin.
		Differentiation is not necessary; however, Clarifier™ 2 should be used to eliminate any background staining caused by the use of excessive water bath adhesive.
		This rapid stain is recommended for frozen sections, requires no filtration prior to use and will not form crystals upon standing or exposure to cold weather.
	www.rallansci.com /signature/series.aspx?id=40 (85 words)

	Histological Stains
		When using hematoxylin, basophilic structures in the tissue appear blue (or purple or brown; this varies according to the stain that is being used).
		A substance that is stained by an acid dye is referred to as acidophilic; it carries basic groups which bind the acid dye.
		A staining sequence involving iron hematoxylin, acid fuchsin, and light green.
	medinfo.ufl.edu /~dental/denhisto/stains.html (1221 words)

	Staining Procedures For Plastic Embedded Tissue
		The mixture is ripe immediately and can be used as long as a film with a metallic luster forms on its surface in a Coplin jar.
		Stain in chromium hematoxylin at 60ºC for 1-2 hours.
		Dissolve the hematoxylin in the alcohol, and the alum in the water by the aid of heat.
	www.ebsciences.com /staining/hematox.htm (614 words)

	Glossary (Site not responding. Last check: 2007-08-08)
		Hematoxylin stains the nucleus, rough endoplasmic reticulum (faintly), and mucopolysaccharides in the extracellular space (a faint bubbly purple color).
		Papanicolaou: The stain named after the cytopathologist who optimized a modified hematoxylin and eosin stain to provide detail needed for cytologic diagnosis.
		The Papanicolaou stain is used to evaluate cervical smears and other cytology specimens, such as fine needle aspirations of cells from the breast.
	mammary.nih.gov /reviews/tumorigenesis/Fischer001/slides/glosry.htm (482 words)

	Prepared Staining Solutions (via CobWeb/3.1 planetlab2.tamu.edu) (Site not responding. Last check: 2007-08-08)
		Gill's Hematoxylin is a specially formulated solution for use as a biological stain for the nuclei and nuclear chromatin of normal and abnormal cells whether in whole or sliced cells.
		At the discretion of the user, the Gill's Hematoxylin No. 2 and No. 3 may be diluted with ethylene glycol (25% is recommended) to produce a weaker stain solution.
		For use with hematoxylin and metanil yellow for the determination of carbohydrates and mucosubstances.
	www.emsdiasum.com.cob-web.org:8888 /microscopy/products/histology/staining_solutions.aspx (346 words)

	Haematoxylin - Wikipedia, the free encyclopedia
		Haematoxylin, hematoxylin, Natural Black 1, or C.I. is extracted from the wood of the logwood tree.
		When oxidised it forms haematein, a compound with rich blue-purple colour, and is used, together with a suitable mordant (most commonly Fe(III) or Al(III) salts), to stain cell nuclei prior to examination under a microscope.
		Before these became established as alternatives to hematoxylin, it returned to the market, albeit at a higher price and resumed its place in histopathology.
	en.wikipedia.org /wiki/Hematoxylin (249 words)

	IMMAGINI (Site not responding. Last check: 2007-08-08)
		The nuclear column (the cells that constitute the body of the microfilaria) is loosely packed, the nuclei can be visualized individually and do not extend to the tip of the tail.
		Giemsa stain, which does not demonstrate the sheath of this sheathed species (hematoxylin stain will stain the sheath lightly).
		The microfilaria is unsheathed, has a nearly straight body attitude, the tail is typically coiled into a “shepherd’s crook”, and terminal nuclei extend as a single row to the end of the tail
	www.chirurgiatoracica.org /immagini6.htm (484 words)

	Procedures - Histology
		Alternatively the section may be stained in alum hematoxylin for 1-2 minutes, blued in Scott's solution, without any application of acid alcohol then stained in a saturated solution of Tratrazine in ethylene glycol monoethyl ether (Cellosolve), 15-30 seconds.
		As this is a routine staining method care should be taken to master the procedure as batches of Hematoxylin and Eosin vary widely, and it requires constant care to secure uniform results.
		Stain in alum hematoxylin for 5 to 15 minutes according to the strength of the hematoxylin solution..
	whitewolf.newcastle.edu.au /techinfo/proc_hist.html (5079 words)

	Stainsfile - Iron hematoxylin - Weigert (via CobWeb/3.1 planetlab2.tamu.edu) (Site not responding. Last check: 2007-08-08)
		The 1904 formula is the solution usually meant when Weigert's iron hematoxylin is specified as an acid resistant nuclear stain.
		The alcoholic hematoxylin solution was originally made by diluting a stock solution of 10% hematoxylin in 95% ethanol.
		Both methods were originally intended to be used without a counterstain for demonstrating chromatin and other structures usually stained by iron hematoxylin.
	www.stainsfile.info.cob-web.org:8888 /StainsFile/stain/hematoxylin/iron/weigert.htm (214 words)

	Biological Stains
		Dissolve the hematoxylin in alcohol, the alum in the water with the aid of heat, and mix the two solutions together.
		Dissolve the Hematoxylin in a little distilled water with the aid of heat.
		Dissolve the hematoxylin in a little water, dissolve the Potassium alum in about 650 mL of distilled water, then mix and make up to 700 mL.
	whitewolf.newcastle.edu.au /techinfo/bio_stains.html (1215 words)

	ROUTINE MAYER'S HEMATOXYLIN AND EOSIN STAIN
		The biggest objection to Mayer's hematoxylin as used in the past, has been that stained slides often fade after I to 3 years.
		If sections are Zenker-fixed, remove the mercuric chloride crystals with iodine and clear with sodium thiosulphate (hypo) (seepage 41).
		Both ferric chloride and iodine serve as mordants, but they also have an oxidizing function that assists in converting hematoxylin to hematein.
	tvmouse.compmed.ucdavis.edu /jaxworkshop/syllabus/stains.htm (2021 words)

	Quantitative Amplification of Genomic DNA from Histological Tissue Sections after Staining with Nuclear Dyes and Laser ...
		lower after hematoxylin staining or when no stain was used.
		Tissue was sampled by manual gross dissection from histological slides (A and B) or by LCM (C and D).
		from hematoxylin by oxidation by sodium iodate) and aluminum ion
	jmd.amjpathol.org /cgi/content/full/3/1/22 (2798 words)

	[No title]
		It is a special stain for the nucleus.
		Hematoxylin, of one formulation or another, is used as a nuclear stain and Eosin is used, either alone or in combination with other plasma stains, as the counterstain.
		The stain theory is based on the attraction of oppositely charged tissue and dye molecules.
	www.statlab.com /Protocol/HistologyStaining.htm (1008 words)

	Engscientific Cytology Page
		(Gill, 1974) Gill Hematoxylin is a high contrast stain and a progressive hematoxylin which enchances the use of counterstains.
		A histologic stain composed of ferric salts which ripen and oxidize hematoxylin rapidly, eliminating artificial or natural ripening.
		II until cytoplasm is free of hematoxylin (check microscopically).
	www.engscientific.com /cyto.html (2911 words)

Try your search on: Qwika (all wikis)