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	HEPES - OpenWetWare
		HEPES contains tertiary amines, which are reactive under certain conditions.
		Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide.
		HEPES is essentially insoluble until it is neutralized.
	openwetware.org /wiki/HEPES (0 words)

	NationMaster - Encyclopedia: Hepes (Site not responding. Last check: )
		Hepes (4-2-hydroxyethyl-1-piperazineethanesulfonic acid) is an organic chemical buffer which is widely used in cell culture to maintain physiological pH.
		Fears that Hepes may serve as a nutrient source for aerobic bacteria have been shown to be unfounded.
		The actual IVF media are not stabilized by HEPES because it may cause ionic changes in embryos and the chances of exposure to atmosphere is less.
	www.nationmaster.com /encyclopedia/Hepes (240 words)

	Paracellular Ion Channel at the Tight Junction -- Tang and Goodenough 84 (3): 1660 -- Biophysical Journal
		, 10 mM glucose, and 10 mM HEPES
		mM glucose, and 10 mM HEPES pH 7.4.
		150 mM NaCl, 10 mM glucose, and 10 mM HEPES pH 7.5; the basal
	www.biophysj.org /cgi/content/full/84/3/1660 (6032 words)

	Science Fair Projects - Hepes
		Hepes (4-2-hydroxyethyl-1-piperazineethanesulfonic acid) is an organic chemical buffer which is widely used in cell culture to maintain physiological pH.
		Hepes is recommended for the protection of frozen solutions of enzymes from freezing-induced pH.
		Fears that Hepes may serve as a nutrient source for aerobic bacteria have been shown to be unfounded.
	www.all-science-fair-projects.com /science_fair_projects_encyclopedia/Hepes (217 words)

	Chromium(VI) Reduction by Ascorbate: Role of Reactive Intermediates in DNA Damage In Vitro
		In HEPES buffer, Cr(V) had a g value of 1.980 and H=1.173 G (where H is the line width measured between the maximum and minimum of the first derivative spectral peak); and in Tris-HCl buffer a much weaker Cr(V) signal was detected at g=1.978.
		HEPES buffer did not react with chromium on this time scale as evidenced by the identical EPR spectra for the same reaction of Cr(VI) with ascorbate carried out in phosphate, dimethylarsenate (cacodylate) and HEPES buffers (10).
		The higher binding of Cr to calf thymus DNA in HEPES vs Tris-HCl buffer is proportional to the increased levels of Cr(V) in HEPES observed by EPR.
	www.ehponline.org /members/1994/Suppl-3/21-25stearns/stearns-full.html (3962 words)

	AppliChem - BioChemica - Chemica - Synthesis - Service (Site not responding. Last check: )
		HEPES zählt zu den Puffern mit den besten Eigenschaften für biologische Untersuchungen (1,3,4).
		In der Zellkultur wird HEPES als Ersatz für den Bicarbonat-Puffer (dann häufig in einer Konzentration von 25 mM) oder als zusätzlicher Puffer zum Bicarbonat (dann häufig in einer Konzentration von 10 - 15 mM) verwendet.
		Einschränkungen in der Verwendbarkeit erfährt HEPES dadurch, daß er nicht eingesetzt werden kann, wenn eine Proteinbestimmung nach Folin durchgeführt werden soll.
	www.applichem.de /catalog/product_details/3261/2706652mynxbcvlaks427453501/HEPESPufferqualitt.htm (274 words)

	Hepes (Site not responding. Last check: )
		HEPES (4-2-hydroxyethyl-1-piperazineethanesulfonic acid) is an organic chemical buffer that is widely used in cell culture to maintain physiological pH.
		It has a pKa of 7.55 at 20 Â°C. HEPES is recommended for the protection of frozen solutions of enzymes from freezing-induced pH.
		For best repeatability of results it is then strongly advised to keep any HEPES containing solution in darkness as much as possible.
	www.guideofpills.com /Hepes.html (267 words)

	HEPES (Solution) - Biocompare Buyer's Guide
		Home » Protein Biochemistry »...(2) » HEPES » HEPES (Solution)
		Click here for comprehensive HEPES (Solution) product listings.
		Quantity: 10X100 ML HEPES, 1.0 M, in saline (0.9%), sterile
	www.biocompare.com /matrix/102/HEPES-(Solution).html (93 words)

	IVF Mail - Using HEPES buffered culture medium in human IVF
		The gamete handling meda (bicarbonate bufferd) are added with HEPES may be due to absorb the shock of pH disturbance due to its prolong exposure to
		We try to minimize the amount of HEPES media that is transferred with our sperm or oocytes; again due to pH alteration.
		If you use oil overlay you should be able to manipulate the zygotes and embryos successfully without the use of HEPES if you only have them out of the incubator for a short period of time.
	www.ivf.net /content/index.php?page=out&id=873 (454 words)

	IRK1 Inward Rectifier K+ Channels Exhibit No Intrinsic Rectification -- Guo and Lu 120 (4): 539 -- The Journal of ...
		The fraction of unblocked currents in the presence of three representative concentrations of HEPES (A), HEP (B), or piperazine (C) is plotted against membrane voltage.
		HEPES is not the sole source of contaminating blockers in commonly
		HEP in the HEPES used to buffer extracellular pH (Figs.
	www.jgp.org /cgi/content/full/120/4/539 (5095 words)

	MEDLINE_1966-1995 - Resultado página 1
		Simultaneous measurement of pH and membrane potential in rat dorsal vagal motoneurons during normoxia and hypoxia: a comparison in bicarbonate and HEPES buffers.
		In bicarbonate ACSF and in 25 mM HEPES ACSF, there was a significant linear relationship between prehypoxic pHi and the direction and amplitude of the hypoxia-induced membrane potential change (either an hyperpolarization or a depolarization).
		Various hypotheses that could give rise to a relationship between changes in membrane potential and pH are discussed; arguments are presented in favor of the concept that modulation of ion channels by either pHi or pHe, or both, is responsible for the observed correlations.
	www.bireme.br /cgi-bin/wxislind.exe/iah/online/?IsisScript=iah/iah.xis&nextAction=lnk&base=MEDLINE_1966-1995&exprSearch=8747226&indexSearch=UI&lang=p (349 words)

	Sarcolemmal mechanisms for pHi recovery from alkalosis in the guinea-pig ventricular myocyte -- Leem and Vaughan-Jones ...
		Similarly, the DIDS-insensitive influx was equivalent to that estimated in Hepes buffer.
		In this case the alkali load was induced by switching from CO to Hepes buffer.
		We conclude that the sarcolemmal acid-loading mechanisms active in Hepes or CO buffer are Cl dependent.
	jp.physoc.org /cgi/content/full/509/2/487 (4817 words)

	LLC-PK1 Cells Stably Expressing the Human Norepinephrine Transporter: A Functional Model of Carrier-Mediated ...
		LLC-NET cells were incubated for 20 min with HEPES buffer in the presence or absence of DMI (100 nM), mazindol (300 nM), 0 mM Na, or reserpine (reserp; 100 nM).
		Preloaded LLC-NET cells were incubated with gramicidin in the absence or presence of EIPA in a physiological HEPES buffer.
		Preloaded LLC-NET cells were incubated with proprionate (25 mM) in the absence or presence of rilminedine with or without idazoxan in a physiological HEPES buffer.
	jpet.aspetjournals.org /cgi/content/full/291/2/456 (4581 words)

	HEPES - OpenWetWare
		HEPES contains tertiary amines, which are reactive under certain conditions.
		Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide.
		HEPES is essentially insoluble until it is neutralized.
	www.openwetware.org /wiki/HEPES (85 words)

	hepes dating ►► Meet Local Women RIGHT NOW! Over 20 Million Members (Site not responding. Last check: )
		hepes dating is celebrated and abored on this website and throughout the known world.
		hepes dating is activley studies at this website.
		If you need assistance with identifying or locating the copyright owner of a work, please contact the owner of the page from which you linked to or found this statement.
	www.dote.adutchat.net /hepesdating.html (291 words)

	Infotrieve Online
		The critical role of Hepes in SIN-1 cytotoxicity, peroxynitrite versus hydrogen peroxide.
		The cytotoxicity of the superoxide anion radical- and nitric oxide-releasing compound SIN-1 to L929 cells was studied in Krebs-Henseleit buffer.
		SIN-1 cytotoxicity was significantly higher in the presence of Hepes than in the absence of Hepes.
	www4.infotrieve.com /newmedline/detail.asp?NameID=9559863&Session=&searchQuery=Rauen&count=267 (215 words)

	British Journal of Pharmacology - Characterization of the anandamide induced depolarization of guinea-pig isolated ...
		The temperature of HEPES buffer was maintained at 37°C by passing solutions through a heat exchanger proximal to the nerve.
		HEPES buffer containing drug solutions were connected via a six channel tap to the perfusion stream.
		In calcium free HEPES buffer the depolarization to anandamide was not significantly different to the response obtained in calcium-containing HEPES buffer (P>0.05).
	www.nature.com /bjp/journal/v137/n1/full/0704840a.html (6494 words)

	[No title]
		Regarding the HEPES addition, I meant that in the past I had been using DMEM, with the addition of FBS, L-glutamine, and Penn-Strep to grow the 3T3cells.
		But as HEPES changes the pH, this could affect transduction itself by interfering with binding of viral particles to the cell membrane.
		I frequently resuspend retrovirus in HEPES buffer, so this should not matter unless the pH is way off.
	www.protocol-online.org /forums/index.php?showtopic=20605&mode=threaded&pid=70937 (641 words)

	Proton-Mediated Feedback Inhibition of Presynaptic Calcium Channels at the Cone Photoreceptor Synapse -- Vessey et al. ...
		Both HEPES and Tris blocked the depolarizing response to 700 nm stimuli and increased the amplitude of the hyperpolarizing response to 660 nm stimuli.
		HEPES (E) and Tris (F) increased the mean response to 660 nm stimuli and inverted the response to 700 nm stimuli.
		HEPES caused an even greater attenuation of the kainate-induced reduction of the Ca signal.
	www.jneurosci.org /cgi/content/full/25/16/4108 (7490 words)

	Spartanburg SC \| GoUpstate.com \| Spartanburg Herald-Journal
		HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is an organic chemical buffering agent that is widely used in cell culture to maintain physiological pH.
		HEPES is recommended for the protection of frozen solutions of enzymes from freezing-induced pH changes.
		For best repeatability of results it is then strongly advised to keep any HEPES containing solution in darkness as much as possible.
	www.goupstate.com /apps/pbcs.dll/section?category=NEWS&template=wiki&text=HEPES (153 words)

	Water Science and Technology 38:7 (1998) 107-115 - Lara L. Zawaideh and Tian C. Zhang - The effects of pH and addition ...
		The feasibility of using zero-valent iron (Fe) powder to remediate nitrate-contaminated water was studied using bench-scale batch reactors.
		Operational parameters, such as Fe dosage (w/v), initial concentration of nitrate-nitrogen, pH, and the use of an organic buffer (HEPES) were studied, specifically focusing on the effects of pH and the addition of HEPES on nitrate transformation using zero-valent iron powder.
		Nitrate-nitrogen was removed by 94% when 0.01M of HEPES was added to a non-shaking batch reactor containing 20 mg/l nitrate-nitrogen and 4% (w/v) of Fe Shaking was proved to be more efficient than no shaking.
	www.iwaponline.com /wst/03807/wst038070107.htm (289 words)

	Polyaromatic compounds and method for their production - Patent 6150491
		An aliquot of enzyme solution, prepared by dissolving horseradish peroxidase (Type II) in N-[2-hydroxyethyl]piperazine-N'[2-ethanesulfonic acid] (HEPES) buffer at a known concentration, is added to the monomer solution such that the final enzyme concentration is 0.1 mg/ml.
		Reaction mixtures were prepared by first dissolving the monomer in HEPES buffer (1 mM to 100 mM, pH 7.5) or a mixture of ethanol and buffer.
		For example, the pH of a 0.1 M solution of 4-HPPA in 0.1 M HEPES buffer (pH 7.5) was 4.3, which is close to the pK.sub.a (about 4.5) of the carboxy group in the monomer.
	www.freepatentsonline.com /6150491.html (3717 words)

	Pore Block Versus Intrinsic Gating in the Mechanism of Inward Rectification in Strongly Rectifying IRK1 Channels -- Guo ...
		I-V curves with HEPES relative to that with phosphate are plotted
		The voltage protocol was as in Fig 1.
		(B) Ratios of the I-V curves with and without HEPES shown in A. The curves superimposed on the data are fits of the equation in Fig 4.
	www.jgp.org /cgi/content/full/116/4/561 (3599 words)

	Estimation of Prostate-Specific Antigen (PSA) Extraction Efficiency from Forensic Samples Using the Seratecâ PSA ...
		The evaluation of the extractions from the stains extracted in 1X HEPES buffer indicated that the dilution that was closest to the 4ng PSA/ml internal standard was the stain prepared with a 1:100 semen dilution.
		It was noted that positive results were not obtained at lower concentrations when using the HEPES buffer despite the three-fold higher estimate of PSA extraction efficiency over extractions using sterile water at the 4ng PSA/ml equivalence level.
		Comparison of the stain and swab extracts with the liquid semen sensitivity results shows that it can be estimated that one percent or less of the PSA applied to the swabs and stains was able to be extracted.
	www.fbi.gov /hq/lab/fsc/backissu/april2003/gartside.htm (1601 words)

	Hydrogen Peroxide Formation by Reaction of Peroxynitrite with HEPES and Related Tertiary Amines. IMPLICATIONS FOR A ...
		HEPES was comparable to the rate of H
		We (14) and others (28) have found that HEPES and SOD influence the decomposition of SIN-1.
		Effect of HEPES and SOD on the SIN-1-induced oxidation of DHR123 and deoxyribose.
	www.jbc.org /cgi/content/full/273/21/12716 (4982 words)

	MEDLINE_1996-2006 - Resultado página 1
		Effects of bicarbonate versus HEPES buffering on measured properties of neurons in the salamander retina.
		Organic pH buffers such as HEPES have become increasingly popular in recent years because for many purposes they offer a convenient and reliable alternative to the more traditional bicarbonate/CO2.
		In this paper, however, we report that important functional properties of rods, bipolar cells, and horizontal cells in the salamander, Ambystoma tigrinum, are sensitive to the choice of buffer and, in the case of horizontal cells, that sensitivity is acute.
	www.bireme.br /cgi-bin/wxislind.exe/iah/online/?IsisScript=iah/iah.xis&nextAction=lnk&base=MEDLINE_1996-2006&exprSearch=9605528&indexSearch=UI&lang=p (272 words)

	The Science Advisory Board
		The reason for adding HEPES is the need for additional buffering.
		what is the reason for adding HEPES buffer to the cell culture medium.
		I am using DMEM for culturing cells, and they seem to grow fine with or without HEPES.
	www.scienceboard.net /forum/post.asp?method=ReplyQuote&REPLY_ID=12096&TOPIC_ID=1177&FORUM_ID=26&CAT_ID=10&Forum_Title=+&Topic_Title=hepes+buffer&M= (381 words)

	hepes free acid (Site not responding. Last check: )
		Storage Temp Hepes Buffer 1M, Free Acid (liquid) SH30237.01 100 mL...
		Descriptions Preparation Note Prepared from HEPES free acid and cell culture...
		We also offer a huge range of organic and inorganic intermediates and we stock consumables for most spectroscopic techniques including NMR, UV, IR and mass spectrometry.
	www.chemindustry.net /chemnames/H/hepes_free_acid.html (276 words)

	[No title]
		HEPES (a commonly used buffer) has a molecular weight of 238.3.
		How many grams of HEPES do you need to weigh out to have one mole?
		Selected Answers: do not look at these until you have tried to answer the questions.
	carbon.cudenver.edu /~bstith/solutionquestions.doc (1233 words)

	info: HEPES (Site not responding. Last check: )
		TOAC Spin Labels in the Backbone of Alamethicin: EPR Studies in Lipid Membranes (RedNova)By Marsh, Derek; Jost, Micha; Peggion, Cristina; Toniolo, Claudio ABSTRACT Alamethicin is a 19-amino-acid residue hydrophobic peptide that produces voltage-dependent ion channels in membranes.
		Hepes Buffered Saline: Available with or without Phenol RedAthenaESÂ provides superior serum-free media cell culture systems for the production of mammalian...
		Used for rinsing cell monolayers prior to cell dissociation; Can be used for washing harvested...
	www.napoli-pizza.net /HEPES.html (296 words)

	The Role of Metabolism, Cytoplasmic Ca[IMAGE], and pH-regulating Exchangers in Glucose-induced Rise of Cytoplasmic pH ...
		In HEPES buffer, glucose concentrations above 7 mM caused an initial rise followed by a secondary decrease and an eventual return to about initial values.
		In HEPES buffer, the increase in release was delayed and displayed a monophasic pattern.
		We therefore cannot support the previous conclusion that the alkalinizing effect of glucose is caused by a stimulation of the Na exchanger(16).
	www.jbc.org /cgi/content/full/270/14/7915 (5487 words)

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