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Topic: Heterodimer


  
 Analysis of heterodimer formation by Xklp3A/B, a newly cloned kinesin-II from Xenopus laevis   (Site not responding. Last check: 2007-10-08)
Figure 4 Heterodimer formation is 'triggered' by the C-terminal part of the stalk.
Figure 5 Heterodimer formation is controlled by a cooperative interaction between coiled-coil regions II and III.
Figure 7 Xklp3A/B heterodimer formation appears to be based on a cooperative mechanism between the C-terminal part of coiled-coil region II and the entire coiled-coil region III.
www.nature.com /emboj/journal/v20/n13/fig_tab/7593835ft.html   (1061 words)

  
 Molecular basis of cooperative DNA bending and oriented heterodimer binding in the NFAT1---Fos-Jun---ARRE2 complex -- ...
DNA bending and the orientation of heterodimer binding.
We have previously shown that the orientation of heterodimer binding affects DNA bending by Fos and Jun (7, 9).
leucine in the leucine zippers on the orientation of heterodimer
www.pnas.org /cgi/content/full/95/14/7915   (5161 words)

  
 The histone acetylase PCAF is a nuclear receptor coactivator -- Blanco et al. 12 (11): 1638 -- Genes and Development   (Site not responding. Last check: 2007-10-08)
One microgram of heterodimer bound to RARE-conjugated beads was incubated with indicated concentrations of 9-cis RA and tested for recruitment of PCAF and p300 (A) from nuclear extracts as in Fig.
Binding of PCAF and GST-SMRT to the heterodimer was detected by anti-M2 antibody and anti-SMRT antibody, respectively.
the heterodimer in the absence of rPCAF, in a ligand-dependent
www.genesdev.org /cgi/content/full/12/11/1638   (6620 words)

  
 W0151: Structural Studies on Full-length Human Replication Protein A Heterodimer   (Site not responding. Last check: 2007-10-08)
It exists in two forms as heterotrimer with three subunits of 70, 32 and 14 kDa and heterodimer of two subunits 32 and 14 kDa.
The heterodimer is known to separate from the heterotrimer upon apoptotic phosphorylation.
The function of human RPA heterodimer is still not well understood due to the lack of a complete structure.
www.hwi.buffalo.edu /ACA/ACA01/abstracts/text/W0151.html   (276 words)

  
 Formation of heterodimers of human-immunodeficiency-virus-type-1 reverse transcriptase by recombination of separately ...
Human-immunodeficiency-virus-type-1 reverse transcriptase exists in virions as a heterodimer of a M(r) 66,000 subunit and its C-terminally truncated form of M(r) 51,000, but, when expressed as a recombinant M(r) 66,000 protein, a mixture of heterodimers and homodimers results which co-purify by most conventional techniques.
The recombined heterodimer showed similar kinetic properties and RNase H activity to the standard heterodimer and a specific activity significantly higher than the original homodimer of the M(r) 66,000 protein.
Heterodimers having greater asymmetry have also been prepared by recombining Mr 66,000 subunits containing single-point or deletion mutations, with wild-type M(r) 51,000 subunits, and the resulting heterodimers analysed.
www.aegis.com /aidsline/1992/sep/M9290043.html   (410 words)

  
 Analysis of heterodimer formation by Xklp3A/B, a newly cloned kinesin-II from Xenopus laevis -- De Marco et al. 20 ...   (Site not responding. Last check: 2007-10-08)
Xklp3A/B heterodimer formation appears to be based on a cooperative mechanism between the C-terminal part of coiled-coil region II and the entire coiled-coil region III.
Heterodimer formation is ‘triggered’ by the C-terminal part of the stalk.
Heterodimer formation is controlled by a cooperative interaction between coiled-coil regions II and III.
embojournal.npgjournals.com /cgi/content/full/20/13/3370   (5327 words)

  
 Analysis of heterodimer formation by Xklp3A/B, a newly cloned kinesin-II from Xenopus laevis
Heterodimer formation is controlled by a cooperative interaction between coiled-coil regions II and III.
As in Figure 4B and C heterodimer formation was monitored by immunoprecipitation with an
In this case slightly shorter stretches are still showing some heterodimer formation.
www.nature.com /emboj/journal/v20/n13/fig_tab/7593835f5.html   (202 words)

  
 Programmed Self-Sorting of Coiled Coils with Leucine and Hexafluoroleucine Cores   (Site not responding. Last check: 2007-10-08)
Unambiguous stepwise synthesis of the heterodimer HF confirms that the disulfide bond-forming chemistry is reversible and under thermodynamic control, and that there are no kinetic barriers to the formation of the disulfide-bonded heterodimer HF.
The mixed heterodimer is estimated to be less than 3% of all H and F containing peptides at equilibrium.
The thermodynamic consequence of the relative stability of the fluorinated peptide assembly FF and the instability of HF is to shift the equilibrium away from the heterodimer to the homodimers.
pubs.acs.org /cgi-bin/jcen?jacsat/123/i47/html/ja016767o.html   (1698 words)

  
 Biosynthesis and Posttranslational Regulation of Human IL-12 -- Carra et al. 164 (9): 4752 -- The Journal of Immunology
in the heterodimer is indistinguishable from the free ß-chain
P5-0.1-derived intracellular CLp67 (a) and secreted SNp74 (b), and monocyte-derived secreted free ß-chain (c) and SNp74 (d) bands were cut out from the gel, reduced, alkylated, pepsin digested, and run on TLC with electrophoresis in the first dimension and ascending chromatography in the second dimension.
); the parallel decrease of the intracellular heterodimer
www.jimmunol.org /cgi/content/full/164/9/4752   (5540 words)

  
 Entomology - Alexander S. Raikhel   (Site not responding. Last check: 2007-10-08)
The functional ecdysteroid receptor is a heterodimer of two nuclear receptors—the ecdysone receptor (EcR) and the retinoid X receptor homolog Ultraspiracle (USP).
The mosquito EcR-USP heterodimer is capable of binding to various EcREs to modulate ecdysone regulation of target genes.
The binding of the EcR/USP heterodimer to the regulatory region of the Vg gene is required for its activation.
www.entomology.ucr.edu /people/raikhel2.html   (1821 words)

  
 [No title]
It is identical in format to the IL-12 heterodimer ELISA described in Basic Protocol 2; however, a capture (coating) antibody is used that recognizes the IL-12 p40 subunit, whether heterodimer-associated or free, instead of the IL-12 heterodimer-specific coating antibody used in Basic Protocol 2.
This Alternate Protocol is not well-suited for measurement of IL-12 heterodimer, as it is known that the p40 subunit of IL-12 is generally produced in excess of the p35 subunit, both in vitro and in vivo.
This represents the approximate fraction of the heterodimer that is p40 protein.
web.tiscali.it /fguidi/measurement_of_il-12.htm   (6149 words)

  
 Macromolecular Structure and Dynamics - NMR Structural Investigations of the Breast Cancer Susceptibility Protein, BRCA1   (Site not responding. Last check: 2007-10-08)
The size of the resulting heterodimeric complex is ~26 kD, which presents certain challenges for NMR structure determination due primarily to problems associated with spectral overlap and signal sensitivity due to unfavorable relaxation properties of larger molecules.
Since BRCA1/BARD1 is a heterodimer, we are able to reduce the spectral overlap problem significantly through the use of heterodimers in which only one protein component is isotopically labeled.
Thus, each half of the heterodimer may be studied independently, affording substantial spectral simplification.
www.emsl.pnl.gov /docs/msd/annual_report1999/1573b-3e.html   (815 words)

  
 OhioLINK ETD: Jacques, Pamela
The heterodimer (p66/p51) is the biologically relevant and the enzymatically active form of RT which initiates minus strand DNA synthesis from the HIV-1 replication primer, human tRNA Lys,3.
These p51 deletion mutants were then reconstituted with wild type HIV-1 p66 in an effort to determine a functional role of p51 in the context of the biologically significant heterodimer.
Each selectively-mutated heterodimer was assayed for retention of DNA- and RNA-dependent DNA polymerase activities, RNase H activity, and template-primer and tRNA Lys,3 primer binding.
www.ohiolink.edu /etd/view.cgi?case1057841989   (350 words)

  
 Excitonic Heterodimer Formation in an HIV-1 Oligonucleotide Labeled with a Donor-Acceptor Pair Used for Fluorescence ...
Excitonic Heterodimer Formation in an HIV-1 Oligonucleotide Labeled with a Donor-Acceptor Pair Used for Fluorescence Resonance Energy Transfer -- Bernacchi et al.
Interaction between the Fl and TMR dyes in the heterodimer results in splitting of the excited state.
For H-type heterodimer, only excitation to the exciton state of highest energy is allowed.
www.biophysj.org /cgi/content/full/84/1/643   (5962 words)

  
 MIT Department of Biology: Frank Solomon   (Site not responding. Last check: 2007-10-08)
The properties of this mutant constitute the first structure-function analysis of the heterodimer itself, and provide a tool for studying the mechanisms by which cells manage tubulin polypeptides.
Promoting formation of the heterodimer: To identify genes that may affect heterodimer formation or stability, we screened for mutations in genes other than those encoding tubulin but that behave as does tub1-724.
It can not be the primary pathway for heterodimer formation, since cells deleted for these genes even in pairwise combinations are viable, but they may represent a salvage pathway promoting reassociation of dissociated heterodimers.
www.mit.edu /~biology/facultyareas/facresearch/solomon.shtml   (1021 words)

  
 Figures   (Site not responding. Last check: 2007-10-08)
Structures of C2B8 (rituximab) homodimer and C2B8/p5E8 heterodimer (rituximab/anti-CD23 or IDEC 152).
This figure depicts the chemical linkage between the homodimerand heterodimer constructs of MAb rituximab and IDEC 152.
It is clear that both the heterodimer and the homodimer have enhanced apoptotic activity.
www.medscape.com /content/2002/00/43/38/433846/433846_fig.html   (233 words)

  
 Structural and functional homology between the RNAPI subunits A14/A43 and the archaeal RNAP subunits E/F -- Meka et al. ...
The E subunit (shown in blue) is an elongated molecule that folds into two domains: a ß-sheet wrapped around a helix (K2) forms the N-terminal domain (at the bottom of the molecule) while the S1 motif in the C-terminal half of the protein folds into an antiparallel ß-barrel with an OB-fold topology.
A weak band, running at a position similar to that of A14/A43–RNA complex, is present also in lane 1 (free probe) and is likely to be due to the formation of secondary structure in the RNA probe.
Both the full-length A14/A43 and the E/F heterodimer bind the RNA probe (as clearly indicated by the formation of a strong band corresponding to the complex and confirmed by the depletion of the free probe), while the formation of the complex was not detected in the presence of the deletion mutants.
nar.oxfordjournals.org /cgi/content/full/31/15/4391   (6060 words)

  
 The vitamin D receptor binds in a transcriptionally inactive form and without a defined polarity on a retinoic acid ...
Mobilities of the receptor heterodimers are indicated by arrows.
in the mobility of the receptor heterodimers (lanes 6 and 11).
Mader, S., Chen, J.-Y., Chen, Z., White, J., Chambon, P., Gronemeyer, H. (1993) The patterns of binding of RAR, RXR and TR homo- and heterodimers to direct repeats are dictated by the binding specificities of the DNA binding domains.
www.fasebj.org /cgi/content/full/13/9/1073   (5376 words)

  
 Ku heterodimer binds to both ends of the Werner protein and functional interaction occurs at the Werner N-terminus -- ...
Ku heterodimer binds to both ends of the Werner protein and functional interaction occurs at the Werner N-terminus -- Karmakar et al.
Ku heterodimer binds to both ends of the Werner protein and functional interaction occurs at the Werner N-terminus
Ku, a heterodimer of full-length Ku70 paired with a fragment
nar.oxfordjournals.org /cgi/content/full/30/16/3583   (5031 words)

  
 The Ku heterodimer is essential for protection of the telomere against nucleolytic and recombinational activity.   (Site not responding. Last check: 2007-10-08)
The Ku heterodimer is essential for protection of the telomere against nucleolytic and recombinational activity.
Binding of the Ku heterodimer to double-stranded DNA breaks has been implicated both in the initiation of DNA replication and in illegitimate recombination through the recruitment of a DNA-dependent protein kinase.
We show here that the yeast homologues of the two subunits of the Ku heterodimer are intrinsically involved in maintaining the integrity of telomere structure.
www.yeastgenome.org /community/meetings/yeast98/abshtml/353.html   (162 words)

  
 A Permissive Retinoid X Receptor/Thyroid Hormone Receptor Heterodimer Allows Stimulation of Prolactin Gene ...
Arrows, mobilities of the heterodimer and the complexes containing the heterodimer and the coactivator (CoA).
(D) Association of the RXR/TR heterodimer with wild-type His-tagged TIF-2 or with TIF-2 with mutations in the second (M2) or third (M3) LXXLL motif (450 ng).
An increase of SMRT binding to the heterodimer in the presence
mcb.asm.org /cgi/content/full/24/2/502   (7941 words)

  
 Characterization of the p68/p58 heterodimer of human immunodeficiency virus type 2 reverse transcriptase.
Characterization of the p68/p58 heterodimer of human immunodeficiency virus type 2 reverse transcriptase.
Recently we demonstrated that the p58 subunit of p68/p58 HIV-2 reverse transcriptase (RT) heterodimer, produced by processing of p68/p68 homodimer with recombinant HIV-2 protease, terminates at Met484 [Fan, N., et al.
The p68/p58 HIV-2 RT heterodimer, produced by specific cleavage using HIV-2 protease, should be useful for inhibition and biophysical studies aimed at discovering and designing drugs directed toward HIV-2.
www.aegis.com /aidsline/1996/sep/M9690697.html   (473 words)

  
 The X-ray Crystal Structure of the NF-kappa B p50{middle dot}p65 Heterodimer Bound to the Interferon beta -kappa B Site ...   (Site not responding. Last check: 2007-10-08)
B p50·p65 heterodimer binds to the major groove of the
B heterodimer containing the murine p50 and p65 subunit Rel homology regions was co-crystallized with a target
heterodimer with a root mean square (rms) deviation of 0.46 Å,
www.jbc.org /cgi/content/full/277/27/24694   (3865 words)

  
 Thyrostimulin, a heterodimer of two new human glycoprotein hormone subunits, activates the thyroid-stimulating hormone ...
Thyrostimulin, a heterodimer of two new human glycoprotein hormone subunits, activates the thyroid-stimulating hormone receptor -- Nakabayashi et al.
Thyrostimulin, a heterodimer of two new human glycoprotein hormone subunits, activates the thyroid-stimulating hormone receptor
Purification of the A2/B5 heterodimer and characterization of its glycoprotein nature.
www.jci.org /cgi/content/full/109/11/1445   (4753 words)

  
 cis-Active Ras G2-like Sequence Implicated in the Heterotropic Activation of the Deoxyadenosine Kinase of Lactobacillus ...
measured was that from the activated dAK subunit of the heterodimer.
Heterotropic activation is not due to the ligand-induced heterodimer formation.
the same as in the native heterodimer (data not shown).
www.jbc.org /cgi/content/full/272/11/6890   (5686 words)

  
 Threading of a glycosylated protein loop through a protein hole: Implications for combination of human chorionic ...
The heterodimer was separated from the free subunits by electrophoresis and the proteins transferred to nitrocellulose (A) or streptavidin-coated nitrocellulose (B).
In addition, the heterodimer was dissociated completely by urea treatment, an indication that no intersubunit disulfide had formed during subunit combination.
Four hours later, when subunit combination was assumed to be complete, excess VP was removed by electrophoresis on a 15% SDS-polyacrylamide gel and proteins were electroeluted from a strip of gel containing the heterodimer and free subunits.
www.proteinscience.org /cgi/content/full/10/2/226   (5139 words)

  
 The Pip2p-Oaf1p heterodimer mediates oleic acid-induced peroxisome proliferation in the yeast Saccharomyces cerevisiae.   (Site not responding. Last check: 2007-10-08)
The Pip2p-Oaf1p heterodimer mediates oleic acid-induced peroxisome proliferation in the yeast Saccharomyces cerevisiae.
This induction is mediated by an upstream activating sequence called ORE (oleate response element) to which binds a heterodimer of the Pip2p and Oaf1p zinc cluster transcription factors.
To elucidate which of the components of the heterodimer receives the oleate signal, we tested LexA -fusions of both proteins for their ability to trans-activate a lexAop-lacZ reporter gene.
www.yeastgenome.org /community/meetings/yeast98/abshtml/610.html   (228 words)

  
 Function of Tubulin Binding Proteins in Vivo -- Fleming et al. 156 (1): 69 -- Genetics
in the presence of cofactor C to release heterodimer.
and the level of heterodimer is correspondingly increased.
-tubulin destabilizes the heterodimer and favors its dissociation.
www.genetics.org /cgi/content/full/156/1/69   (5690 words)

  
 Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15 -- Fribourg ...   (Site not responding. Last check: 2007-10-08)
Herold, A., Klymenko, T. and Izaurralde, E. (2001) NXF1/p15 heterodimers are essential for mRNA nuclear export in Drosophila.
Izaurralde, E. (2002) A novel family of nuclear transport receptors mediates the export of messenger RNA to the cytoplasm.
Strasser, K., Bassler, J. and Hurt, E. (2000) Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export.
emboreports.npgjournals.com /cgi/content/full/4/7/699   (2734 words)

  
 rBAT-b0,+AT heterodimer is the main apical reabsorption system for cystine in the kidney -- Fernández et al. 283 ...
AT are expressed solely as heterodimers in brush-border membranes (where they are localized).
AT heterodimer is present in human and mouse brush-border membranes.
AT heterodimers: implications for cystine transport and reabsorption in the kidney.
ajprenal.physiology.org /cgi/content/full/283/3/F540   (5740 words)

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