| | Title of Invention: Method for production of petros elinic acid and OMEGA12 hexadecanoic acid in transgenic plants |
 | | To determine the position of the double bond in the new peak of hexadecanoic acid and confirm the identity of the putative methyl petroselinate peak, monounsaturated fatty acid methyl esters of the total lipids from transgenic calli were purified by argentation TLC (Morris, L. J., Wharry, D. and Hammond, E. Chromatog. |
 | | To demonstrate that petroselinic acid and cis-4-hexadecanoic acid could be reproducibly detected in transgenic callus of tobacco, leaf discs of rooted, transformed plants were placed on media described lacking antibiotics. |
 | | It is also possible that the amino acid and corresponding nucleotide sequence of the.omega.12 acyl-ACP desaturase may be used to engineer a wide range of acyl-ACP desaturases that have the ability to catalyze the insertion of double bonds at a variety of positions in the carbon chain of fatty acids. |
| www.nal.usda.gov /bic/Biotech_Patents/1995patents/05430134.html (7148 words) |