
	Where results make sense

Topic: Hoechst stain

Related Topics

Hoechst Uclaf

In the News (Thu 16 Feb 12)

EXECUTIVE POWER

Iran

ANALYSIS

Pakistan

Family compact

	Reference.com/Encyclopedia/Staining
		Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes.
		Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.
		Gram staining uses crystal violet to stain cell walls, iodine as a mordant, and a fuchsin or safranin counterstain to mark all bacteria.
	www.reference.com /browse/wiki/Staining_%28biology%29 (2085 words)

	Invitrogen - Molecular Probes - Section 8.6 - Nuclear and Chromosome Counterstaining and Nissl Stains
		The Hoechst 33342 dye is commonly used in combination with labeling by 5-bromo-2'-deoxyuridine (BrdU, B23151) to distinguish the compact chromatin of apoptotic nuclei, to identify replicating cells and to sort cells based on their DNA content (Section 15.4, Section 15.5).
		Hoechst 33342 is commonly used to distinguish the compact chromatin of apoptotic nuclei, in combination with BrdU labeling to identify replicating cells and to sort cells based on DNA content (Section 15.5).
		Staining with the SYTO 11 dye was used to follow the movement of cells during development in whole-mount zebrafish embryos.
	probes.invitrogen.com /handbook/sections/0806.html (3974 words)

	Hoechst stain - Wikipedia, the free encyclopedia
		The Hoechst stains are part of a family of fluorescent stains for labelling DNA in fluorescence microscopy.
		The Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI.
		Because the Hoechst stains bind to DNA, they can disrupt DNA replication during cell division.
	en.wikipedia.org /wiki/Hoechst_stain (163 words)

	UD Biological Sciences - Multiphoton Applications
		Simultaneous NLO acquisition of the UV dye Hoechst 33342 for DNA (blue), and confocal imaging of FlAsH to monitor gene correction (Green) and Texas Red (red) to visualize chimera in living cultured cells.
		Simultaneous multiphoton imaging of whole mouse lenses stained with DAPI and Alexa 488 Phalloidin using 810nm excitation.
		A multi-celled moouse embryo loaded with the vital DNA stain Hoechst 33342 to label nuclei were imaged under continuous laser illumination (730nm excitation) over a 10 minute period (32 slices per z-stack).
	www.udel.edu /bio/research/facilities/microscopy/equipment/multiapps.html (529 words)

	Protocols (Site not responding. Last check: 2007-10-15)
		The Hoechst purification was established for murine HSC on normal C57Bl/6 bone marrow (NBM).
		Both of these are found to stain a small proportion of the total bone marrow, but stain the SP cells brightly and distinctly, and thus allow separate confirmation of the identification of the SP region.
		The Hoechst dye is excited with the UV laser at 350 nm and its fluorescence measured with a 450 BP 20 and a 675 EFLP optical filter (Omega Optical, Brattleboro VT).
	www.bcm.edu /genetherapy/goodell/page2.htm (1001 words)

	Invitrogen - Molecular Probes - Section 8.1 - Nucleic Acid Stains
		Hoechst dyes are used in many cellular applications, including cell-cycle and apoptosis studies (Section 15.4, Section 15.5) and they are common nuclear counterstains (Section 8.6).
		The Hoechst 33258 and Hoechst 33342 dyes are available as solids (H1398, H1399), as guaranteed high-purity solids (FluoroPure Grade; H21491, H21492) and, for ease of handling, as 10 mg/mL aqueous solutions (H3569, H3570).
		Staining by the Nissl stains is completely eliminated by pretreatment of tissue specimens with RNase; however, these dyes are not specific stains for RNA in solutions.
	probes.invitrogen.com /handbook/sections/0801.html (7159 words)

	biostatus - faq's
		For imaging and nucleated cell tracing on flow cytometry we see staining within 30 seconds but for cell cycle flow analyses we would take the staining out to 10-15 min at 37 deg to ensure that equilibrium has been attained.
		Hoechst 33342 (or the less lipophilic form Hoechst 33258) is a useful agent for the in vitro DNA labelling of live or fixed cells.
		The spectral properties of the Hoechst dyes are: UV-excitable, with broad emission in the visible range from violet extending to red.
	www.biostatus.co.uk /faqs.html (2055 words)

	FLICA Assay
		Cells were washed, then Hoechst stain was added and the cells were incubated for 5 minutes.
		Nuclear staining by Hoechst stain on the right (photo B) was revealed using a UV-filter (excitation at 365 nm, emission at 480 nm).
		The cell in the bottom left of photo B (which is not visible in photo A) does not have a brightly stained nucleus, therefore it is neither apoptotic nor necrotic.
	www.gentaur.com /flica_assay.htm (349 words)

	Respiratory Research \| Full text \| Apoptosis and the activity of ceramide, Bax and Bcl-2 in the lungs of neonatal rats ...
		Hoechst (10 mg/ml) was diluted to 1 μg/ml, in which slides were incubated for 5 minutes, and then washed in phosphate buffered saline (PBS) for 45 minutes.
		TUNEL staining was used to determine the level of apoptosis in experimental and control rats and to generate an apoptotic index, which was 0.65 for the lungs of hyperoxia-exposed rats at 3 days.
		Hoechst staining, on the other hand, is utilized to document apoptosis by visualizing chromatin condensation and nuclear shrinkage followed by the formation of apoptotic bodies.
	respiratory-research.com /content/7/1/100 (5885 words)

	Cytotoxicity Assays
		When stained, the live cells show up as green coloured cells, whereas the cells with cytotoxicity and those with compromised cell membrane functions show red colouration of the nuclei.
		The cytoplasm of cells with intact plasma membrane are stained green, and apoptotic (late stages) as well as necrotic cells are stained red.
		The cells in early stages of apoptosis with intact plasma membrane show the typical apoptotic nuclear morphology stained blue with Hoechst dye, while the cytoplasm are stained green.
	cometassay.com /index_files/CytotoxicityAssays.htm (507 words)

	Rengarajan, Mol Vis 2002; 8:416-421.
		This study examined four fluorophores (ethidium bromide, Hoechst 33258, PicoGreen, and SYBR Green I), each with characteristics that may be helpful or a hindrance in different situations.
		In contrast, Hoechst 33258 is non-intercalating, apparently binding to the minor groove of DNA with a marked preference for AT sequences [1].
		Hoechst 33258 stock solution (Molecular Probes Inc., Eugene, OR; catalog number H3569) was diluted 1:400 in TNE buffer to give a concentration of 0.025 mg/ml.
	www.molvis.org /molvis/v8/a49 (1992 words)

	mycoplasma testing (Site not responding. Last check: 2007-10-15)
		This procedure is based on the observation of a fluorescent staining pattern of DNA in the cytoplasm of indicator cells (VERO) that are known to be mycoplasma negative.
		The proper staining pattern and color indicates the presence of mycoplasma contaminating the surface of the cells that would have occurred from the sample.
		That is, very "clean" preparations that stain negative are almost always accurate, whereas preparations with cell debris or lysed cells can contribute to artifact background staining and could lead to a positive reading.
	www.unc.edu /depts/tcf/mycoplasma_testing.htm (419 words)

	Fluorescence Microscopy
		The staining pattern of AO (green fluorescence for DNA, red fluorescence for RNA) is a function of the dye concentration, which has to be adjusted in such a way that AO bound to DNA is a monomeric intercalation form, whereas AO bound to RNA is a complex of dye polymers and the RNA.
		The absorption of AO is in between 440 and 480 nm (blue), the emission is in between 520 nm (green for DNA) and 650 nm (orange for RNA).
		Hoechst 33342 (bisbenzimide H 33342) is a specific stain for AT-rich regions of double-stranded DNA like DAPI and also the fluorescence properties are similar to DAPI.
	www.micro-scope.de /fluoro.html (1016 words)

	[No title]
		The blue filter you are using is fine for the Hoechst blue emission; however the red emission is far weaker and the bandpass you are using may be too narrow; I normally would have a long pass (620LP generally).
		When I set up voltages for SP (on a MoFlo), because Hoechst staining is very bright, the population of cells is often off-scale so I have to bring the voltages down to be able to view it on the display graph.
		Hoechst at 450/20 BP and PI at 670/40 or LP, fine tune with PMT voltages and keep in linear for both parameters.
	www.cyto.purdue.edu /MD-parts/6a53ac21a35d77d37abd7de9bc943cadc909b3c2.doc (1339 words)

	UNIT 4: DNA ISOLATION AND CHARACTERIZATION
		Hoechst 33258 is therefore extremely useful for estimating the DNA content of crude cellular extracts, from which RNA has not been removed.
		Silver staining is rapid and approximately 100x as sensitive as ethidium bromide staining which makes it ideal for detecting weak bands after PCR amplification.
		Once the gel is stained with ethidium bromide the band of interest can be obtained by exposing the gel briefly to a hand held UV light and quickly cutting out the band of interest with a razor blade or scalpel.
	www.med.unc.edu /wrkunits/3ctrpgm/pmbb/mbt/DNA-ISOL.htm (15436 words)

	[No title] (Site not responding. Last check: 2007-10-15)
		Hoechst 33258 is a bis-benzimidazole fluorescent compound with activity against schistosomes and other protozoan parasites.
		QM-stained preparations show more stable fluorescence than preparations stained with Q, therefore, the mustard functionality is important for the speificity of staining.
		The report that QM stains chromosomes more durably than Q begs the question why Q is active at all.
	weisblumlab.pharmacology.wisc.edu /306.htm (1477 words)

	BioMed Central \| Full text \| Effects of chemokines on proliferation and apoptosis of human mesangial cells
		Apoptosis was studied with several assays: Flow cytometric cell cycle analysis was performed using propidium iodide staining as described previously [17].
		Staining with Hoechst visualizes cells with fragmented chromatin.
		In immunohistochemistry we found a clear staining pattern for SLC/CCL21 on podocytes and CCR7 on MC during nephrogenesis and in adult kidney.
	www.biomedcentral.com /1471-2369/5/8 (4329 words)

	COLORANTES SIN … MODIFICADO (Site not responding. Last check: 2007-10-15)
		Multiple stain is a replacement for the former Parangon Multiple Stain (PMS).
		The stain can be directly applied to frozen section, epoxy or JB-4TM embeddeed sections and utilized as a general cytoplasmic and nuclear stain.
		A nonspecific fluorescent stain for detecting and identifying fungi and algae in tissue.
	www.prolab.cl /productos/produc_anat./colorantes_pc.html (399 words)

	DNA quantification using Picogreen
		dsDNA Quantitation Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures.
		Hoechst (bisbenzimide) dyes are sensitive fluorescent nucleic acid stains that circumvent many of these problems.
		The Hoechst 33258 - based assay is somewhat selective for dsDNA, does not show significant fluorescence enhancement in the presence of proteins and allows the detection and quantitation of DNA concentrations as low as 10 ng/mL DNA.
	www.topac.com /picogreen.html (1268 words)

	BioForum > help chosing a fluorescent DNA stain
		Hoechst gives a much cleaner more crisp stain than the DAPI in the mounting media.
		Dec 1 2006, 05:13 AM Info I find on Hoechst is usually with xylene and alcohol washes before a 5 minute stain in Hoechst.
		Hoechst staining — added a 1:100 dilution (diluted in PBS) of Hoechst (10mg/ml) to the appropriate slides.
	www.protocol-online.org /forums/lofiversion/index.php?t22523.html (595 words)

	BIAF - DNA staining method
		These fluorescent stains are used to primarily label double-stranded DNA and they bind to A-T regions of the DNA helix.
		Hoechst 33342 has the advantage of being cell membrane permeant and can, therefore, stain nuclei in living cells as well as fixed cells.
		Hoechst 33258 (MW 624.0), Hoechst 33342 (MW 615.9).
	www.biaf.uwa.edu.au /biaf/DNAstains.htm (283 words)

	Epstein-Barr Virus BZLF1 Protein Binds to Mitotic Chromosomes -- Adamson 79 (12): 7899 -- The Journal of Virology
		NIH 3T3 cells were transfected with a Z expression plasmid and stained with Hoechst stain (A) and an anti-Z antibody (B).
		D98-HE/R1 cells were transfected with a Z expression plasmid and stained with Hoechst stain (C) and an anti-Z antibody (D).
		The DNA was stained with Hoechst stain (A, C).
	jvi.asm.org /cgi/content/full/79/12/7899 (3374 words)

	Frank's Science Pages
		Frequency histogram of DNA content (in cytometric measurements equivalent to DNA stain fluorescence) - analysis of DNA frequency histograms reveals the proportion of the algal population within the cell cycle phases G1 (non dividing cells), S (DNA replication), and G2+M (Mitosis and cell division).
		The new DNA staining technique allows for the first time to measure both DNA and chlorophyll fluorescence simultaneously on an argon laser based bench top cytometer with blue light excitation.
		With bench top machines (that could be taken at sea), equipped only with blue light excitation (488 nm), DNA with the red fluorescent dyes propidium iodide or ethidium bromide involved methanol extraction of chlorophyll prior to DNA analysis as chlorophyll exhibits red fluorescence as well.
	www.jochemnet.de /dnaback.html (748 words)

	handprint : synthetic organic pigments
		Developed and patented by Hoechst in 1960, the benzimidazolones were first used as watercolor pigments in the late 1970's.
		Naphthols are nontoxic, often extremely saturated, and in watercolors are semitransparent and strongly staining pigments.
		All the perylenes are nontoxic, mid valued, transparent and strongly staining pigments with very good to excellent lightfastness in watercolors (many are also used as automotive colors).
	www.handprint.com /HP/WCL/pigmt1d.html (4251 words)

	Translocation of the Zinc Finger Protein Basonuclin from the Mouse Germ Cell Nucleus to the Midpiece of the ...
		A, C, and E are basonuclin stains; B, D, and F are basonuclin stains superimposed on Hoechst stains.
		Note in cells near the basement membrane, the area defined by basonuclin stain follows closely that of the DNA stain (arrow); but in spermatids near the lumen, whose chromatin condensation is apparent, the area of basonuclin stain is larger than that of the DNA stain (arrowhead).
		The region framed in A is enlarged and shown as DNA stain (Hoechst 33258) superimposed on basonuclin stain (B).
	www.biolreprod.org /cgi/content/full/59/2/388 (4276 words)

	4.5 Hoechst 33258 direct DNA stain for Mycoplasma
		Cell cultures are stained with Hoechst 33258, a fluorescent stain, which binds specifically to DNA.
		The Hoechst stain also detects bacterial and fungal contamination, although these are usually obvious from the visible turbidity of the culture medium of infected cells.
		Since the Hoechst stain will stain all DNA, the nucleus of all cells should also stain brightly.
	www.who.int /vaccines/en/poliolab/webhelp/Chapter_04/4_5_Hoechst_33258_direct_DNA_stain_for_Mycoplasma.htm (528 words)

	Direct Identification and Enrichment of Retinal Stem Cells/Progenitors by Hoechst Dye Efflux Assay -- Bhattacharya et ...
		Freshly isolated E18 retinal cells were stained with Hoechst 33342 dye in the presence or absence of verapamil.
		The Hoechst dye staining and emission patterns of retinal cells revealed that the efflux of Hoechst dye by SP cells took place in the absence (A) and in the presence (B) of verapamil.
		SP and NSP cells, isolated from neurospheres by Hoechst dye efflux assay (D), were subjected to RT-PCR analysis to detect transcripts corresponding to progenitor- (nestin), neuron- (Map2), glia- (GFAP), and retina- (opsin and syntaxin 1) specific cell markers (E).
	www.iovs.org /cgi/content/full/44/6/2764 (5145 words)

	Tissue Culture Facility (Site not responding. Last check: 2007-10-15)
		This procedure is based on the observation of a fluorescent staining pattern of DNA in the cytoplasm of indicator cells (VERO) that are known to be mycoplasma negative.
		The proper staining pattern and color indicates the presence of mycoplasma contaminating the surface of the cells that would have occurred from the sample.
		That is, very "clean" preparations that stain negative are almost always accurate, whereas preparations with cell debris or lysed cells can contribute to artifact background staining and could lead to a positive reading.
	www.unclineberger.org /tcf/mycoplasma.html (431 words)

	Preparation and Staining of Mammal Cells to View Sister Chromatids
		It is a lengthy protocol but it produces slides of mammalian chromosomes that are easily observed under a good high power microscope lens and worth the effort for those wanting to observe mammalian chromosomes.
		Slides may be stained with Giemsa stain (3% Giemsa in phosphate buffer for 10 min.) to see chromosomes.
		Differential staining with fluorescence -Hoechst (bisbenzamide) and Giemsa for observing sister chromatid exchanges may be done instead.
	www.accessexcellence.org /AE/AEC/AEF/1994/geach_cell.html (512 words)

Try your search on: Qwika (all wikis)