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Topic: Holoenzyme

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	Nat' Academies Press, (NAS Colloquium) Links Between Recombination and Replication: Vital Roles of Recombination (2002)
		The bacteriophage T4 DNA polymerase holoenzyme is derived from the polymerase (gp43), the clamp (gp45), and the clamp-loader complex (gp44/62) (1, 2).
		Analogous proteins are found in both the Escherichia coli holoenzyme, consisting of the DNA polymerase III, the β clamp, and the clamp-loading γ complex, and in the eukaryotic holoenzyme, consisting of DNA polymerase δ, the proliferating cell nuclear antigen (PCNA) clamp, and the clamp-loading RF-C complex (3–7).
		The final state of the holoenzyme was observed by mixing 2 µM of the gp45H-gp44/62-DNA complex formed in the presence of 2 mM ATP with 2 µM of gp43 in complex buffer.
	www.nap.edu /books/030907424X/html/197.html (8244 words)

	MCM Proteins Are Associated with RNA Polymerase II Holoenzyme -- Yankulov et al. 19 (9): 6154 -- Molecular and Cellular ...
		The holoenzyme fraction from a GST-TFIIS affinity column (0.325 M NaCl eluate) was fractionated on a Sepharose CL-2B column.
		Copurification of Pol II holoenzyme and MCMs by cation-exchange chromatography, sucrose gradient sedimentation, and TFIIS affinity chromatography.
		of the holoenzyme by anti-MCM3 or to incomplete elution from the
	mcb.asm.org /cgi/content/full/19/9/6154 (7519 words)

	DNA polymerase III holoenzyme - Wikipedia, the free encyclopedia
		DNA polymerase III holoenzyme is the primary enzyme complex involved in prokaryotic DNA replication.
		Being the primary holoenzyme involved in replication activity, the DNA Pol III holoenzyme also has proofreading capabilities that correct replication mistakes by means of exonuclease activity working 3'->5'.
		DNA Pol III is a component of the replisome, which is located at the replication fork.
	en.wikipedia.org /wiki/DNA_polymerase_III_holoenzyme (247 words)

	Amino-terminal sequences of sigma N (sigma 54) inhibit RNA polymerase isomerization -- Cannon et al. 13 (3): 357 -- ...
		The interaction of holoenzymes with melted DNA in stable complexes was examined by use of S1 nuclease.
		Holoenzyme complexes lacking Region I are transcriptionally active on heteroduplex DNA without activator.
		in holoenzyme that is needed to unmask a holoenzyme single-strand
	www.genesdev.org /cgi/content/full/13/3/357 (5356 words)

	Structural Basis of Transcription Initiation: RNA Polymerase Holoenzyme at 4 A Resolution -- Murakami et al. 296 ...
		Holoenzyme was reconstituted by mixing a 1.2-fold molar excess of
		Holoenzyme solution (15 mg/ml) was mixed with the same volume of crystallization solution containing 0.1 M Hepes-NaOH, pH 8.0, 3 M sodium formate, and crystal seeds.
		segment in the holoenzyme crystals is indicated above the bar, with rectangles indicating the three structured domains, and lines representing the flexible linkers.
	www.sciencemag.org /cgi/content/full/296/5571/1280/DC1 (1367 words)

	Escherichia coli RNA polymerase core and holoenzyme structures (Site not responding. Last check: 2007-10-14)
		The thumbs and ledge of the holoenzyme are held apart by the wedge-shaped density, which, in the third dimension, is placed above the rest of the surrounding density.
		NTD dimer in the holoenzyme (Figure 5) is supported by two further lines of evidence.
		The integrity of the purified polymerases was determined by native gel assays (Gallegos and Buck, 1999).
	www.nature.com /emboj/journal/v19/n24/full/7593496a.html (6463 words)

	Reconstitution of the holoenzyme form of Escherichia coli porphobilinogen deaminase from apoenzyme with porphobilinogen ...
		Reconstitution of the holoenzyme form of Escherichia coli porphobilinogen deaminase from apoenzyme with porphobilinogen and preuroporphyrinogen: a study using circular dichroism spectroscopy.
		Reconstitution of the holoenzyme form of Escherichia coli porphobilinogen deaminase from apoenzyme with porphobilinogen and preuroporphyrinogen: a study using circular dichroism spectroscopy.The results showed clearly that the cofactor was generated much more rapidly from preuroporphyrinogen than from PBG.
		The CD spectrum of the holoenzyme was found to be similar at both pH 5.1 and 7.4, suggesting that the crystal structure, determined at pH 5.1, is likely to be similar at physiological pH values.
	www.pdg.cnb.uam.es /UniPub/iHOP/gp/1091779.html (340 words)

	Histone Acetyltransferase and Protein Kinase Activities Copurify with a Putative Xenopus RNA Polymerase I Holoenzyme ...
		The Pol III holoenzyme is self-sufficient for transcription of
		Pol I holoenzymes as it is in Xenopus.
		The RNA polymerase II holoenzyme and its implications for gene regulation.
	mcb.asm.org /cgi/content/full/19/1/796 (7599 words)

	Interaction of Drosophila DNA polymerase alpha holoenzyme with synthetic template-primers containing mismatched primer ...
		Interaction of Drosophila DNA polymerase alpha holoenzyme with synthetic template-primers containing mismatched primer bases or propanodeoxyguanosine adducts at various positions in template and primer regions.
		Interaction of Drosophila DNA polymerase alpha holoenzyme with synthetic template-primers containing mismatched primer bases or propanodeoxyguanosine adducts at various positions in template and primer regions.Similar results were obtained when PdG was placed at various positions in the primer region.
		We studied recognition and binding of synthetic template-primers by Drosophila DNA polymerase alpha (pol alpha) holoenzyme.
	www.pdg.cnb.uam.es /UniPub/iHOP/gp/70097.html (281 words)

	Conformational Flexibility in sigma 70 Region 2 during Transcription Initiation -- Anthony and Burgess 277 (48): 46433 ...
		Holoenzymes containing the indicated disulfide bond were purified by Ni-NTA chromatography followed by immunoaffinity chromatography using a monoclonal antibody NT73 to the C terminus of the
		holoenzymes was due to the presence of the disulfide bond in
		The activity of the purified mutant holoenzymes was measured by multiple-round transcription using a supercoiled lacUV5 promoter as described under "Experimental Procedures." The results are the averages of three different experiments.
	www.jbc.org /cgi/content/full/277/48/46433 (5542 words)

	The DnaX-binding Subunits delta ' and psi Are Bound to gamma and Not tau in the DNA Polymerase III Holoenzyme -- Glover ...
		The DnaX complex subassembly of the DNA polymerase III holoenzyme is comprised of the DnaX proteins
		Holoenzyme protein subunit standards were subjected to SDS-PAGE and Western blot analysis as described under "Experimental Procedures." A-D represent a single lane containing 10 pmol of each holoenzyme protein subunit blotted with a single monoclonal antibody directed against DnaX complex subunits (anti-
		of the cross-linked holoenzyme lanes (3 and 4) with cross-linked
	www.jbc.org /cgi/content/full/275/5/3017 (2560 words)

	Binding of bisubstrate analog promotes large structural changes in the unregulated catalytic trimer of aspartate ...
		In contrast, the PALA-liganded holoenzyme has been thought to represent the R state structure (5, 9).
		The similarity of the liganded conformations indicates that PALA binding is sufficient to promote the closed hinge in the holoenzyme.
		Studies of the holoenzyme by UV absorption spectroscopy revealed distinct structural alterations caused by the binding of
	www.pnas.org /cgi/content/full/97/10/5077 (3470 words)

	BIOL414/614 at UMBC - Lyudmila_Bard_JE2a (Site not responding. Last check: 2007-10-14)
		In the second system, the holoenzyme was isolated from !GAL11P strain of yeast and the activator lacks an activating domain but has instead a portion of Gal4 dimerization region that binds to a mutant holoenzyme !GAL11P.
		When holoenzyme concentration is doubled, transcription levels with and without activator are higher but the degree of activation is lower.
		When holoenzyme concentration is 4 times higher than the original one, the basal level of transcription is the same as level of activated transcription.
	www.umbc.edu /bioclass/biol414/wiki/edit.php?page=Lyudmila_Bard_JE2a (850 words)

	Escherichia coli RNA polymerase core and holoenzyme structures (Site not responding. Last check: 2007-10-14)
		One of the density matching rotations is shown in (vi).
		(v) and (vi) are displayed with transparent 'wedge' and 'protrusion' density, necessary to prevent this region of the holoenzyme obscuring the placement of
		flexible flap was bent back by some 90° in the holoenzyme when compared with the core subunits, upon the binding of the
	www.nature.com /emboj/journal/v19/n24/fig_tab/7593496f6.html (335 words)

	Transcription Initiation-Defective Forms of sigma 54 That Differ in Ability To Function with a Heteroduplex DNA ...
		inhibitory effect of region I on the holoenzyme (10, 26, 30).
		change in the holoenzyme and responsiveness to the activator (10).
		Holoenzymes formed with L37P or L333P were capable of efficient activator-independent transcription from the heteroduplex
	jb.asm.org /cgi/content/full/182/22/6503 (3346 words)

	RNA Polymerase II Holoenzyme Modifications Accompany Transcription Reprogramming in Herpes Simplex Virus Type ...
		HSV-1 infection alters the composition of the RNAP II holoenzyme.
		RNAP II holoenzymes from infected cells are transcriptionally inactive.
		In this study, we report that the RNAP II holoenzyme is depleted of TFIIE and is transcriptionally inactive after HSV-1 infection.
	jvi.asm.org /cgi/content/full/75/20/9872 (8757 words)

	Interaction of the Bacillus subtilis RNase P with the 30S ribosomal subunit -- BARRERA and PAN 10 (3): 482 -- RNA
		The holoenzyme dimer and monomer are well separated, but the dissociation of the dimer during gel electrophoresis generates a smear between the dimer and the monomer.
		Because 30S binding does not affect the catalytic activity of the RNase P holoenzyme on this pre-tRNA substrate, it was certain that all pre-tRNA substrates had been cleaved before the sample was loaded onto the native gel.
		For each position, the amount of radioactivity in the absence of 30S subunit divided by the amount of radioactivity in the presence of 30S subunit is shown as closed squares.
	www.rnajournal.org /cgi/content/full/10/3/482 (5625 words)

	In vitro roles of invariant helix-turn-helix motif residue R383 in {{sigma}}54 ({{sigma}}N) -- Wigneshweraraj et al. 29 ...
		Native gel holoenzyme assembly assays were used to detect complexes forming between core RNAP and R383K and R383A, respectively.
		holoenzyme function than one with a T at –13 (33).
		R383K holoenzyme transcribed from the glnHp2-13T and glnHp2-13T
	nar.oxfordjournals.org /cgi/content/full/29/5/1163 (6736 words)

	Ionic interactions between PRNA and P protein in Bacillus subtilis RNase P characterized using a magnetocapture-based ...
		is similar to that of the holoenzyme in the absence of substrate
		Characterization of the ionic interaction of the holoenzyme complex at high ionic strength.
		Chamberlain, J.R., Lee, Y., Lane, W.S., and Engelke, D.R. Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP.
	www.rnajournal.org /cgi/content/full/10/10/1595 (7249 words)

	Creating a dynamic picture of the sliding clamp during T4 DNA polymerase holoenzyme assembly by using fluorescence ...
		We had previously used the V163C mutant to develop a complex kinetic mechanism of holoenzyme assembly (23).
		Presteady-state fluorescence values were measured for each state of the holoenzyme assembly following Scheme 1.
		The final state of the holoenzyme was observed by mixing 2 µM of the gp45
	www.pnas.org /cgi/content/full/98/15/8368 (5040 words)

	Intermediates in formation and activity of the RNA polymerase II preinitiation complex: holoenzyme recruitment and a ...
		Intermediates in formation and activity of the RNA polymerase II preinitiation complex: holoenzyme recruitment and a postrecruitment role for the TATA box and TFIIB -- Ranish et al.
		Although the exact composition of the holoenzymes are variable, a number of observations support the holoenzyme recruitment
		In the presence of Gal4-AH, the D-A-promoter complex is required for the stable recruitment of the Srb/Mediator holoenzyme.
	www.genesdev.org /cgi/content/full/13/1/49 (8637 words)

	Association of Herpes Simplex Virus Type 1 ICP8 and ICP27 Proteins with Cellular RNA Polymerase II Holoenzyme -- Zhou ...
		Role of nucleic acids in association of HSV proteins with the Pol II holoenzyme.
		II holoenzyme (79), we demonstrated that the HSV
		Purification and characterization of RNA polymerase II holoenzyme from Schizosaccharomyces pombe.
	jvi.asm.org /cgi/content/full/76/12/5893 (8364 words)

	PKA holoenzyme is functionally coupled to CFTR by AKAPs -- Huang et al. 278 (2): 417 -- AJP - Cell Physiology
		PKA holoenzyme is functionally coupled to CFTR by AKAPs -- Huang et al.
		PKA holoenzyme in isolated membrane patches is bound to AKAPs.
		Because the principal hallmark of PKA holoenzyme is
	ajpcell.physiology.org /cgi/content/full/278/2/C417 (4280 words)

	The Base Substitution and Frameshift Fidelity of Escherichia coli DNA Polymerase III Holoenzyme in Vitro -- Pham et al. ...
		We have investigated the in vitro fidelity of Escherichia coli DNA polymerase III holoenzyme from a wild-type and a proofreading-impaired
		Data are normalized per unit of pol III holoenzyme.
		Fidelity of pol III Holoenzyme during in Vitro Gap-filling DNA Synthesis-- To measure the fidelity of the HE we used a forward mutational assay using the N-terminal region of the lacI gene as mutational
	www.jbc.org /cgi/content/full/273/36/23575 (6774 words)

	RNA Polymerase Holoenzyme Architecture
		The holoenzyme is composed of the catalytic core (subunits
		The molecular weight of the holoenzyme is 480 kDa.
		The enzyme is shown bound to a fragment of DNA that contains most of the sequence-specific elements required for transcription initiation.
	www.bio.cmu.edu /Courses/03231/ProtStruc/1L9Z.htm (185 words)

	The N-terminal region of DNA polymerase {delta} catalytic subunit is necessary for holoenzyme function -- Schumacher et ...
		The N-terminal region of DNA polymerase {delta} catalytic subunit is necessary for holoenzyme function -- Schumacher et al.
		The RF-C-dependent holoenzyme assay was carried out as described in Materials and Methods.
		is active in a RF-C-dependent holoenzyme assay in the presence of E.coli SSB.
	nar.oxfordjournals.org /cgi/content/full/28/2/620 (2958 words)

	holoenzyme - OneLook Dictionary Search
		Tip: Click on the first link on a line below to go directly to a page where "holoenzyme" is defined.
		Holoenzyme : Drug Discovery and Development [home, info]
		Phrases that include holoenzyme: dna polymerase iii holoenzyme, poliii holoenzyme
	www.onelook.com /?w=holoenzyme (144 words)

	Welcome to the School of Biological Sciences (Site not responding. Last check: 2007-10-14)
		Shoolingin-Jordan, P.M., Warren, M.J. and Awan, S.J. (1996) Discovery that the assembly of the dipyrromethane cofactor of porphobilinogen deaminase holoenzyme proceeds initially by the reaction of preuroporphyrinogen with the apoenzyme.
		Awan, S.J., Siligardi, G., Shoolingin-Jordan, P.M. and Warren, M.J. Reconstitution of the holoenzyme form of Escherichia coli porphobilinogen deaminase from apoenzyme with porphobilinogen and preuroporphyrinogen: A study using circular dichroism spectroscopy.
		Shoolingin-Jordan, P.M., Warren, M.J. and Awan, S.J. (1997) Dipyrromethane cofactor assembly of porphobilinogen deaminase: Formation of apoenzyme and preparation of holoenzyme.
	www.sbs.soton.ac.uk /staff/pmsj/pmsj.htm (2624 words)

	A Domain of RecC Required for Assembly of the Regulatory RecD Subunit Into the Escherichia coli RecBCD Holoenzyme -- ...
		A Domain of RecC Required for Assembly of the Regulatory RecD Subunit Into the Escherichia coli RecBCD Holoenzyme -- Amundsen et al.
		holoenzyme, free RecC, and a small amount of RecBC (lanes 4–5).
		a conformational change or be released from the holoenzyme at
	www.genetics.org /cgi/content/full/161/2/483 (5006 words)

	Genetic Analysis of the Role of Pol II Holoenzyme Components in Repression by the Cyc8-Tup1 Corepressor in Yeast -- Lee ...
		Genetic Analysis of the Role of Pol II Holoenzyme Components in Repression by the Cyc8-Tup1 Corepressor in Yeast -- Lee et al.
		Genetic Analysis of the Role of Pol II Holoenzyme Components in Repression by the Cyc8-Tup1 Corepressor in Yeast
		, 1995 A kinase-cyclin pair in the RNA polymerase II holoenzyme.
	www.genetics.org /cgi/content/full/155/4/1535 (4749 words)

	The flagellar anti-sigma factor FlgM actively dissociates Salmonella typhimurium sigma 28 RNA polymerase holoenzyme -- ...
		The flagellar anti-sigma factor FlgM actively dissociates Salmonella typhimurium sigma 28 RNA polymerase holoenzyme -- Chadsey et al.
		FlgM is believed to prevent RNA polymerase (RNAP) holoenzyme
		holoenzyme destabilization, enhances the sensitivity of the cell
	www.genesdev.org /cgi/content/abstract/12/19/3123 (409 words)

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