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Topic: Hybridomas

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	Monoclonal antibody production using hybridomas
		Hybridomas will be subcloned to ensure that cell lines are monoclonal.
		The Antibody Core Facility will provide between 10 and 20 hybridoma cell lines, which are shown positive to the stated peptide or protein by ELISA screening.
		The successful generation of hybridomas, secreting monoclonal antibodies with specific performance traits, is based solely on probability.
	www.utsouthwestern.edu /utsw/cda/dept218066/files/219913.html (752 words)

	Altweb: Alternatives to Monoclonal Antibody Production (Proceedings)
		Therefore the question is not whether hybridomas can be grown in vitro, but rather, whether they can be grown in culture at densities and for sufficient periods of time to yield the needed quantity and concentration of antibody for its desired purpose.
		Growth of hybridomas in cell culture is subject to microbial contamination and mechanical failure is possible when producing antibody in a HFB.
		(1980) Monoclonal Antibodies Hybridomas: A new dimension in biological analyses.
	altweb.jhsph.edu /meetings/mab/lipman.htm (3554 words)

	Hybridomas for lung cancer marker and monoclonal antibodies thereof - Patent 6117981
		Hybridomas secreting monoclonal antibodies specific for an epitope found in the amino acids of LCGA associated with non-small cell lung carcinoma protein have been found.
		The monoclonal antibodies produced by these hybridomas can be used in in vivo and in vitro clinical diagnosis of non-small cell lung carcinoma and ovarian carcinoma and as target selective carriers for various anti-tumor agents and radioimaging agents.
		In another aspect, the present invention is a hybridoma cell line which produces a monoclonal antibody which binds to non-small cell lung carcinoma cell antigen LCGA and which has the epitope binding specificity of an antibody produced by the hybridoma cell line 3C2-E7-F10 (ATCC NO. HB-12566).
	www.freepatentsonline.com /6117981.html (6945 words)

	Identification of antigens derived from Cryptococcus neoformans that stimulate T cell responses. (Site not responding. Last check: 2007-10-19)
		Hybridomas were screened for reactivity with crude cryptococcal protein preparations by a bioassay which measured IL-2 production in the presence of antigen-presenting cells.
		Following immunization of mice with live and glass bead- disrupted C. neoformans, 48 and 65 hybridomas were generated, of which 26 and 28, respectively, reacted with C. neoformans.
		Thus, using T cell hybridoma technology, we have purified a protein from C. neoformans that stimulates a T cell response.
	gateway.nlm.nih.gov /MeetingAbstracts/102195667.html (410 words)

	Method of producing hybridomas (EP0508472A2)
		Hybridomas producing a monoclonal antibody of the IgG class are produced by cultivating a hybridoma producing a monoclonal antibody belonging to the IgM class in the presence of an antigen reactive with the IgM antibody, and in the presence of thymocytes.
		This hybridoma production method makes it easy to obtain monoclonal antibodies of the IgG class to a glycolipid or the like to which monoclonal antibodies of the IgG class are difficult to obtain.
		This invention relates to a method of producing hybridomas which comprises converting (class-switching) a hybridoma capable of producing a monoclonal antibody belonging to the IgM class to a hybridoma capable of producing a monoclonal antibody belonging to the IgG class.
	www.delphion.com /details?pn=EP00508472A2 (352 words)

	Monoclonal antibody specific for a mammary tumor cell surface antigen - Patent 5032521
		Hybridomas are provided which secrete an IgG1 or IgG2 monoclonal antibody which binds to an epitope on an antigen, which occurs in the plasma membrane of MCF-7 cells and has a molecular weight of 22 kD.
		Monoclonal antibody from one hybridoma culture, designated 15A8, is reactive with a mammary tumor cell surface antigen and is useful for diagnosing mammary tumors and, as a part of an immunotoxin (e.g., conjugated with a toxin such as gelonin), is potentially useful therapeutically as an agent against mammary tumors.
		The invention entails hybridomas and cultures thereof, antibodies made by the hybridomas and cultures of the invention, and methods of making and using the hybridomas, hybridoma cultures, and antibodies of the invention.
	www.freepatentsonline.com /5032521.html (3642 words)

	Proceedings of the Production of Monoclonal Antibodies Workshop
		In addition to murine hybridomas, one may produce mixed species, or "heterohybridomas." Heterohybridomas are created by fusing splenocytes obtained from a species other than a mouse with a murine plasmacytoma.
		Mice syngeneic to the hybridoma, or, alternatively, immunocompromised stocks or strains, are inoculated intraperitoneally with a priming agent, typically pristane, 7-21 days prior to intraperitoneal inoculation of hybridoma cells.
		Our reasoning is: most hybridomas will grow and produce a suitable quality and quantity of antibody in vitro; one cannot predict whether a specific hybridoma will fail to produce antibody in vitro; technology is available to most laboratories for this purpose; and in vivo production may lead to pain or distress.
	altweb.jhsph.edu /topics/mabs/ardf/jackson.htm (6896 words)

	[No title]
		Hybridomas (maximum 15 per fusion) judged to be secreting specific MAbs will be cloned, and the pre-clone supernatants then provided to the customer for their evaluation.
		For service 2 the customer is encouraged to supply each hybridoma under simple code, and the customer must be prepared to supply (also under code) 50-200 ug of specific antigen relevant for each hybridoma (also under code).
		Should a hybridoma prove refractory to secreting MAb within the normal concentration range; should CMI be unable to rectify the problem; should the customer be unable to provide reagents or information to correct the problem, then CMI retains the right to terminate the contract at no charge to the customer.
	www.cmi.rcip.com /Services1.doc (1988 words)

	HYBRIDOMA CORE - Services
		Sub-Cloning and Cryopreservation: Hybridomas assaying positive for desired antibody secretion are grown in successively larger wells and then subcloned in the presence of thymocyte feeder cells. After growth of subclones the supernatants are rescreened. Selected subclones are then expanded and cryopreserved in liquid nitrogen.
		Human-Human Hybridomas: These have been successfully produced in this facility using methods described by Rauch (8). These methods, which use the human B lymphoblastoid line GM4672 as the fusion partner, as well as other methods, will be available on an ongoing basis in this core facility.
		Rabbit Hybridomas: The Hybridoma Facility will be acquiring the rabbit plastocytoma cell line 240E1. Fusions of splenocytes from immunized rabbits with this cell line have successfully generated stable hybridomas secreting monoclonal rabbit antibodies (9).
	www.med.umich.edu /mdrtc/textonly/hybridomaCore/services.htm (388 words)

	P-252 (Site not responding. Last check: 2007-10-19)
		Furthermore, the hybridomas produced utilizing the NSO myeloma cell line of the invention have greater resistance to death.
		The hybridomas produced by this process are more resistant to cell death than B cells producing hybridomas alone.
		In addition, these hybridomas secrete monoclonal antibodies which are high affinity, and therefore bind more effectively to foreign antigens than do the low-affinity monoclonal antibodies produced by B cells, as well as to autoantibody-producing cells.
	www.aecom.yu.edu /home/biotechnology/p252.htm (150 words)

	Hybridoma Shared Research Facility - Mount Sinai School of Medicine
		The Hybridoma Center provides a complete hybridoma development program designed to produce hybridoma cell lines that secrete monoclonal antibodies specific to your antigen.
		If the serum titers are deemed acceptable by both the Hybridoma Center and the client, animals will then receive a final boost three to four days prior to a fusion intravenously (IV) via the tail.
		Screening: Hybridoma cultures are screened to identify colonies secreting monoclonal antibodies specific to the target antigen.
	www.mssm.edu /research/resources/hybridoma (526 words)

	The Johns Hopkins Center for AIDS Research
		Generation of new hybridomas secreting monoclonal antibodies against novel antigens or epitopes.
		In addition to hybridomas provided to the Core by CFAR investigators for antibody production, the very large hybridoma library already established by the Core director will be available to CFAR investigators.
		CFAR investigators can have new hybridomas generated by the CORE by submitting a variety of immunogens including plasmid DNA.
	www.hopkinsmedicine.org /aidsresearch/monoclonalabcore.asp (253 words)

	Ultrastructural and Immunologic Characteristics of Mouse x Cattle Xenogeneic Hybridomas Originating from Bovine ...
		hybridomas were frozen in 95% bovine fetal calf serum and 5%
		The hybridomas were grown in RPMI 1640 medium,
		Cytoplasm of BLV5B8 hybridoma cells is mostly occupied by RER.
	www.vetpathology.org /cgi/content/full/40/4/460 (1763 words)

	The Wistar Institute :: Anti-cancer Antibodies Specific to Tumor-associated Antigens Expressed on All Epithelial ...
		Wistar scientists have isolated numerous antibodies secreted by hybridomas derived from the immunization of mice with live tumor cells.
		Hybridomas with restricted reactivities were selected, subcloned and the secreted antibodies have been tested on a variety of tumor and normal cells.
		Antibody CO56-45 was secreted by hybridomas derived from mice immunized with a human rectal carcinoma cell line.
	www.wistar.org /tech_transfer/reagents/anti2b.html (657 words)

	Downmodulation of the Inflammatory Response to Bacterial Infection by {gamma}{delta} T Cells Cytotoxic for Activated ...
		The proportion of hybridomas with increased FSC and SSC after culture with PECs is shown in the gated population.
		Hybridomas were labeled with CFSE and cultured for 24 h in the presence of media alone, unsorted low density PECs (43% PECs), or the sorted PEC populations (R1 and R2).
		hybridomas declined to 8 and 4% in the presence of 48 and 58%
	www.jem.org /cgi/content/full/191/12/2145 (6776 words)

	MDRTC Hybridoma Core
		The fusion partner used for murine hybridomas is the P3X63-Ag8.653 cell line developed by Kearney et al (3).
		Hybridomas are plated in a highly enriched selection medium and macroscopic colonies appear within seven to 10 days. In approximately two weeks the medium is acidified and ready for screening by the investigator’s laboratory.
		This procedure may be introduced in the Hybridoma Core on an experimental basis in the future: please inquire regarding the status of the technology.
	www.med.umich.edu /MDRTC/cores/hybridomaCore/services.htm (442 words)

	Hybridomas Congresses. - Microbiology - What's Been Published (Site not responding. Last check: 2007-10-19)
		Hybridomas and cellular immortality / edited by Baldwin H. Tom and James P. Allison.
		Lymphocyte hybridomas : Second Workshop on Functional Properties of Tumors of T and B Lymphocytes / sponsored by the National Cancer Institute (NIH), April 3-5, 1978, Bethesda, Maryland, U.S.A. ; edited by F. Melchers, M. Potter, N. Warner.
		In vitro immunization in hybridoma technology : proceedings of the International Symposium on In Vitro Immunization in Hybridoma Technology, Tylösand, Sweden, September 7-8, 1987 / edited by Carl A.K. Borrebaeck.
	www.pitbossannie.com /rps-qr-hybridomas-congresses.html (123 words)

	Hybridomas. - Microbiology - What's Been Published
		Monoclonal antibodies : hybridomas, a new dimension in biological analyses / edited by Roger H. Kennett and Thomas J. McKearn and Kathleen B. Bechtol.
		Hybridoma technology in the biosciences and medicine / edited by Timothy A. Springer.
		Electromanipulation in hybridoma technology : a laboratory manual / edited by Carl A.K. Borrebaeck and Inger Hagen.
	www.pitbossannie.com /rps-qr-hybridomas.html (176 words)

	T Cell Response to Preproinsulin I and II in the Nonobese Diabetic Mouse -- Halbout et al. 169 (5): 2436 -- The Journal ...
		The stimulation index is indicated for each T cell hybridoma screened as the ratio between IL-2 production in the presence of islet cells vs that in the absence of islet cells.
		Hybridomas responding with a stimulation index of 3 were considered islet cell specific.
		A and B, T cell hybridomas that gave a positive response in the presence of peptide pools 1, 2, 3, or 4 tested in the first screening were reevaluated against each individual peptide contained in the pool.
	www.jimmunol.org /cgi/content/full/169/5/2436 (5214 words)

	Generation of Hybridomas
		Transfer hybridomas from positive wells to 24 well plates in HT medium.
		Hybridomas in the 24 well plates are accurately counted and then 60 hybridomas are suspended in 15 ml HT medium plus 5 ml thymocytes and transfer 200 ml/well in the 96-well plates.
		All hybridomas must be cloned at least 2 times.
	www.ibms.sinica.edu.tw /~sroff/protocols/hybridoma.htm (411 words)

	Hitendra S. Jethwa Dissertation Abstract (Site not responding. Last check: 2007-10-19)
		A total of 20 hybridomas were generated by fusing splenocytes from 5 unimmunized SCG mice.
		Tissue culture supernatant from all 20 hybridomas bound to MPO by enzyme-linked immunosorbent assay (ELISA).
		Upon purification of the immunoglobulin fraction of the supernatants under high-salt conditions, the MPO binding capacity of the “dual specific” supernatants was abrogate whereas that of the 13 supernatants that bound to MPO only remained.
	www.pathology.unc.edu /graduate/jethwa-dissertation-abstract.htm (416 words)

	Identification of immunodominant T cell epitopes of human glutamic acid decarboxylase 65 by using HLA-DR(alpha ...
		T cell hybridomas were "washed" by aspiration of culture medium and resuspension in fresh HAT medium.
		For each peptide, the sequence and the number of responding hybridomas is shown.
		All 14 hybridomas respond to p551-565 and p566-580.
	www.pnas.org /cgi/content/full/94/15/8082 (4310 words)

	Methods for producing and culturing rabbit-mouse hybridomas and monoclonal antibodies secreted by rabbit-mouse ...
		[0004] Rabbit-mouse hybridomas that secrete rabbit monoclonal antibodies (mAb) may be produced by fusing rabbit immunocytes, such as splenocytes, with mouse myeloma cells.
		In certain embodiments, the method comprises culturing the hybridoma in a medium comprising IL-6 or a functional equivalent thereof.
		In certain embodiments, the method further comprises transferring the at least one stable rabbit-mouse hybridoma to a growth medium lacking IL-6 or a functional equivalent thereof.
	www.freshpatents.com /Methods-for-producing-and-culturing-rabbit-mouse-hybridomas-and-monoclonal-antibodies-secreted-by-rabbit-mouse-hybridomas-dt20051229ptan20050287644.php (1172 words)

	Identification of an I-Ed-Restricted T-Cell Epitope of Escherichia coli Outer Membrane Protein F -- Williams and Bigley ... (Site not responding. Last check: 2007-10-19)
		Hybridomas were unable to recognize antigen presented by transfected L cells expressing I-A
		The boundaries of the minimal sequence required for stimulation of hybridoma cells, peptide 295-314, were sequentially reduced in size by one amino acid from the amino and carboxy termini, and these smaller peptides were tested for the ability to stimulate IL-2 secretion.
		The minimal sequence recognized encompassed the core amino acid sequence FEVGATYYF (residues 295 to 303); however, a minimum of three additional amino acids is required at either the amino or carboxy end of the core sequence.
	iai.asm.org /cgi/content/full/72/7/3907 (4757 words)

	Antibody & Cell Culture, GRASP Digestive Diseases Center
		Hybridoma development by the core has three phases which take 4-6 months to complete.
		Once hybridoma colonies reach ~300 cells, the plates are assayed by ELISA to determine which colonies are secreting antibodies to the immunogen.
		Hybridoma cloning (5-10 weeks): When the assessment by the investigators is complete, the selected hybridomas are thawed from cryogenic storage and placed into culture.
	www.tufts.edu /med/GRASP/cellculture-custom.html (650 words)

	Supplemental Data for: (Site not responding. Last check: 2007-10-19)
		Hybridoma DNA digested with Stu1 reveals a 6.9 kb band in the NS-1 DNA.
		The IgM-secreting hybridoma, clone M, and genomic wt DNA hybridize with a 15 kb band.
		EcoR1-digested DNA from IgG1-secreting hybridomas (1-13), and an IgM secreting hybridoma derived from DP-T/HC/LC B cells, CTR is a IgG1-secreting wt/HC/LC hybridoma, TC-1 is a 129 derived ES cell line, and NS-1 is the myeloma fusion partner.
	www.immunity.com /cgi/content/full/16/4/607/DC1 (628 words)

	Loyola Univ. Health Univ. Health Sys- Microbiology & Immunology: Knight Lab Protocals - Rabbit Hybridomas - Method ...
		Another way to avoid or at least to minimize this problem, is to incubate the spleen cells for 24-48 h on plastic dishes to remove adherent cells before fusion.
		If still adherent cells grow and interfere with the growth of the hybridomas it is necessary to transfer the hybridomas into another well as soon as possible.
		The hybridomas are cloned by limited dilution in 48-well microtitre plates.
	www.meddean.luc.edu /lumen/DeptWebs/MICROBIO/knight/PROTOC/HYBRIDOM.HTM (967 words)

	(WO/1996/030030) DENDRITIC-LIKE CELL/TUMOR CELL HYBRIDS AND HYBRIDOMAS FOR INDUCING AN ANTI-TUMOR RESPONSE (Site not responding. Last check: 2007-10-19)
		The hybrids and hybridomas are generated by the fusion of tumor cells with dendritic-like cells.
		Dendritic-like cell/tumor cell hybridomas and pluralities of hybrids can be directly infused for active immunization of cancer patients against their residual tumor cells.
		The hybridomas and hybrids can also be used for the in vitro activation of autologous immune cells before their reinfusion into the patient for passive immunization against the tumor cells.
	www.wipo.int /pctdb/en/wo.jsp?WO=1996/30030 (523 words)

	Alibris: Hybridomas
		In 1984 Cesar Milstein and Georges Kohler were awarded the Nobel Prize for their discovery of "hybridoma technology," a method leading to the production of unlimited quantities of biological reagents of "exquisite specificity" known as monoclonal antibodies.
		Lymphocyte Hybridomas: Second Workshop on Functional Properties of Tumors of T and B Lymphocytes
		Includes all of the information required to produce monoclonal antibodies in the laboratory and to prepare them for use in a multitude of given applications.
	www.alibris.com /search/books/subject/Hybridomas (353 words)

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