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Topic: Hydrogenase

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Nickel

Hydrometallurgy

	Characterization of a Cytosolic NiFe-Hydrogenase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1 -- ... (Site not responding. Last check: 2007-10-03)
		Hydrogenase of the hyperthermophile Pyrococcus furiosus is an elemental sulfur reductase or sulfhydrogenase: evidence for a sulfur-reducing hydrogenase ancestor.
		Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction.
		Biochemical and molecular characterization of the [NiFe] hydrogenase from the hyperthermophilic archaeon Thermococcus litoralis.
	jb.asm.org /cgi/content/full/185/5/1705 (3280 words)

	Pathways of H^sub 2^ toward the Active Site of [NiFe]-Hydrogenase Biophysical Journal - Find Articles
		Hydrogenases have received much attention due to their potential to replace expensive platinum in conventional fuel cells that catalyze the oxidation of hydrogen.
		Hydrogenases are natural catalysts that some organisms have and use in the reversible conversion of molecular hydrogen without the help of expensive metals such as platinum.
		There are various types of hydrogenases assisting the reversible oxidation of H^sub 2^, being mostly found in archaea and bacteria, but also in eucarya (hydrogenosomes of protozoa and chloroplasts of green algae) (7).
	www.findarticles.com /p/articles/mi_qa3938/is_200609/ai_n16731343 (1109 words)

	Membrane-bound hydrogenase and sulfur reductase of the hyperthermophilic and acidophilic archaeon Acidianus ambivalens ... (Site not responding. Last check: 2007-10-03)
		Membrane-bound hydrogenase and sulfur reductase of the hyperthermophilic and acidophilic archaeon Acidianus ambivalens -- Laska et al.
		The hydrogenase and SR subunits deduced from the genome sequence of Acidithiobacillus
		The membrane-bound hydrogenase, the SR and the soluble hydrogenase
	mic.sgmjournals.org /cgi/content/full/149/9/2357 (6464 words)

	Theoretical Calculations on Hydrogenase Kinetics: Explanation of the Lag Phase and the Enzyme Concentration Dependence ... (Site not responding. Last check: 2007-10-03)
		Hydrogenases are metalloenzymes that catalyze the reaction H
		Theoretical simulation of hydrogenase activity kinetic measurements of fully activated hydrogenase reaction using both the conventional and the autocatalytic triangular model.
		Theoretical simulation of hydrogenase activity kinetic measurements of partially activated hydrogenase reaction using both the nonautocatalytic and the autocatalytic triangular model.
	www.biophysj.org /cgi/content/full/89/3/1957 (3556 words)

	A multisubunit membrane-bound [NiFe] hydrogenase and an NADH-dependent Fe-only hydrogenase in the fermenting bacterium ... (Site not responding. Last check: 2007-10-03)
		Amino-terminal sequences of the 40 kDa and the 14 kDa subunits of Ech hydrogenase from Ta.
		not in the proximity of the hydrogenase genes.
		Characterization of the region encoding the CO-induced hydrogenase of Rhodospirillum rubrum.
	mic.sgmjournals.org /cgi/content/full/150/7/2451 (5331 words)

	Gas Transport inside hydrogenase CpI
		Hydrogenases constitute a family of enzymes found in certain microorganisms, which catalyzes the reversible oxidation of hydrogen gas.
		If harnessed properly, hydrogenase and/or hydrogenase-containing organisms could be used to supply affordable and renewable H2 to be used as an energy fuel, and thus solve the "supply" aspect of the future hydrogen economy.
		Swallowed by a protein: the fantastic voyages of O2 and H2 In order to understand how O2 is transported across hydrogenase to reaches the H-cluster, we generated an all-atom simulation model based on the PDB crystal structure of CpI hydrogenase (1FEH).
	www.ks.uiuc.edu /Research/hydrogenase (1355 words)

	Hydrogenases and Hydrogen Metabolism of Cyanobacteria -- Tamagnini et al. 66 (1): 1 -- Microbiology and Molecular ... (Site not responding. Last check: 2007-10-03)
		While the uptake hydrogenase is present in all nitrogen-fixing strains tested so far, the bidirectional enzyme is distributed among both nitrogen-fixing and non-nitrogen-fixing cyanobacteria (although it is not a universal cyanobacterial enzyme) (192).
		The molecular masses indicated for the uptake hydrogenase subunits are mean values calculated from the deduced amino acid sequences of Anabaena strain PCC 7120 (39), Nostoc strain PCC 73102 (150), and A.
		homologous to xisC and to the bidirectional hydrogenase genes.
	mmbr.asm.org /cgi/content/full/66/1/1 (7701 words)

	E.coli hydrogenase maturating endopeptidase
		A conformational change occurs after cleavage in which the consensus sequence bound nickel atom is brought to the interior of the large subunit and forms a metal center with an iron atom that is bound at the N-terminus of the large subunit.
		This conformational change allows for the hydrogenase small subunit to complex with the large subunit resulting in a mature and active hydrogenase (4).
		Theodoratou, E., Paschos, A., Magalon, A., Fritsche, E., Huber, R., and Bock, A. (2000) Nickel serves as a substrate recognition motif for the endopeptidase involved in hydrogenase maturation Eur.
	www.arches.uga.edu /~joscott/bcmb8010 (427 words)

	Chapter 5 - Hydrogen production
		Hydrogenase is thought to become active in order to excrete excess reducing power under specific conditions, such as anaerobic conditions.
		Hydrogenase is too oxygen-labile for sustainable hydrogen production: light-dependent hydrogen production ceases within a few to several tens of minutes since photosynthetically produced oxygen inhibits or inactivates hydrogenases.
		Hydrogenases have been purified and partially characterized in a few cyanobacteria and microalgae (9).
	www.fao.org /docrep/w7241e/w7241e0g.htm (2426 words)

	Structural studies of nickel-iron hydrogenase
		Albracht, S.P.J. The use of electronparamagneticresonance spectroscopy to establish the properties of nickel and the iron-sulphur cluster in hydrogenase from Chromatium vinosum.
		Cammack, R., Bagyinka, C. and Kovacs, K.L. a) Spectroscopic characterization of the nickel and iron-sulphur clusters of hydrogenase from the purple photosynthetic bacterium Thiocapsa roseopersicina.
		Roberts, L.M. and Lindahl, P.A. Analysis of oxidative titrations of Desulfovibrio gigas hydrogenase: implications for the catalytic mechanism.
	metallo.scripps.edu /PROMISE/NIFE_REF.html (2011 words)

	Engineering the Rhizobium leguminosarum bv. viciae Hydrogenase System for Expression in Free-Living Microaerobic Cells ... (Site not responding. Last check: 2007-10-03)
		operon of the UPM791 hydrogenase cluster is induced in microaerobiosis
		hydrogenase is induced in soybean bacteroids and in microaerobic
		Nucleotide sequence of the genetic loci encoding subunits of Bradyrhizobium japonicum uptake hydrogenase.
	aem.asm.org /cgi/content/full/68/5/2461 (4247 words)

	Krasna Lab (Site not responding. Last check: 2007-10-03)
		E.coli synthesizes three hydrogenase isoenzymes, each of which are composed of two large catalytic subunits and two small subunits required for electron transport to and from substrates.
		The approaches being used involve isolation of mutant strains of bacteria with altered hydrogenase activities, defining the mutation and cloning the genes which restore activity to the mutant strains.
		Chaudhari, A. and Krasna, A.I. Restoration of hydrogenase activity in hydrogenase-negative strains of Escherichia coli by cloned DNA fragments from Chromatium vinosum and Proteus vulgaris.
	convex.hhmi.columbia.edu /krasna/krasna_group.html (356 words)

	Hydrogenase - Wikipedia, the free encyclopedia
		Until 2004, hydrogenases were classified according to the metals thought to be at their active sites; three classes were recognized: iron-only (Fe-only), nickel-iron (NiFe), and "metal-free".
		The active site, which is believed to be the place where catalysis takes place, is also a metallocluster, and each metal is coordinated by carbon monoxide (CO) and cyanide (CN) ligands.
		Peters, J.W., Lanzilotta, W.N., Lemon, B.J. and Seefeldt, L.C. "X-ray crystal structure of the Fe-only hydrogenase (CpI) from Clostridium pasteurianum to 1.8 Å resolution".
	en.wikipedia.org /wiki/Hydrogenase (723 words)

	Energy Challenge [Detailed]: Biohydrogen Production
		A vast majority of organisms that contain hydrogenases have not been identified and probably cannot be cultured in the laboratory using current procedures.
		Studying hydrogenase function within the context of a network maintained by living cells is essential to understanding how this process is influenced by different pathways and environmental conditions.
		Tools for monitoring hydrogenase activity in vivo and integrating diverse sets of experiment data are needed to build in silico models of a biophotolytic organism under hydrogen producing conditions.
	genomicsgtl.energy.gov /benefits/biohydrogen.shtml (1989 words)

	Nickel-iron hydrogenase
		The Ni-Fe hydrogenases (EC 1.18.99.1) are heterodimeric proteins consisting of small (S) and large (L) subunits.
		The EPR studies of Ni-Fe hydrogenases have shown the existence of three paramagnetic states called NiA, NiB and NiC; other forms of the hydrogenase called NiR, NiSI and NiSU are EPRsilent (see above; note that the actual oxidation states of the Ni and Fe in all the various intermediates remain to be determined experimentally).
		Forms R, C and SI participate in the catalytic cycle of the hydrogenase, while A, SU and B are involved in the activation and inactivation of the enzyme [3]:
	metallo.scripps.edu /PROMISE/NIFE.html (1007 words)

	Hydrogen Production. Green Algae as a Source of Energy -- Melis and Happe 127 (3): 740 -- PLANT PHYSIOLOGY (Site not responding. Last check: 2007-10-03)
		The mature Fe hydrogenase polypeptide is depicted by a green linear rectangle.
		Meyer J, Gagnon J (1991) Primary structure of hydrogenase I from Clostridium pasterianum.
		Peters JW, Lanzilotta WN, Lemon BJ, Seefeldt LC (1998) X-ray crystal structure of the Fe-only hydrogenase (CpI) from Clostridium pasteurianum to 1.8 angstrom resolution.
	www.plantphysiol.org /cgi/content/full/127/3/740 (4703 words)

	Cyanobacterial-Type, Heteropentameric, NAD+-Reducing NiFe Hydrogenase in the Purple Sulfur Photosynthetic Bacterium ... (Site not responding. Last check: 2007-10-03)
		Structural genes coding for the pentameric NiFe hydrogenase are indicated by solid arrows, apparently Hox-related genes are indicated by cross-hatched arrows, and unrelated putative genes are indicated by grey arrows.
		Evidence against a common use of the diaphorase subunits by the bidirectional hydrogenase and by the respiratory complex I in cyanobacteria.
		Cloning and nucleotide sequences of the genes for the subunits of NAD-reducing hydrogenase of Alcaligenes eutrophus H16.
	aem.asm.org /cgi/content/full/70/2/722 (4595 words)

	International Hydrogenase Conference 2004 (Site not responding. Last check: 2007-10-03)
		The cyanide Ligands of [NiFe] Hydrogenases in E. coli: From HypE to the HypC x HypD Complex.
		Maturation and biodiversity of hydrogenases from legume endosymbiotic bacteria.
		The multistep maturation pathway of membrane-bound [NiFe] hydrogenase.
	www.hydrogenase.reading.ac.uk /programme.html (277 words)

	Selective release and function of one of the two FMN groups in the cytoplasmic NAD+-reducing [NiFe]-hydrogenase from ... (Site not responding. Last check: 2007-10-03)
		In the Desulfovibrio gigas enzyme, the two hydrogenase subunits are bound via a large hydrophobic contact surface [15].
		Coremans, J.M.C.C., Van der Zwaan, J.W. and Albracht, S.P.J. (1989) Redox behavior of nickel in hydrogenase from Methanobacterium thermoautotrophicum (strain Marburg).
		Axley, M.J., Keefe, R.G., Falk, M.C. and Harabin, A.L. (1995) FMN cofactor dissociation from the soluble hydrogenase of Alcaligenes eutrophus H16.
	content.febsjournal.org /cgi/content/full/271/4/801 (4050 words)

	Iron-Dependent Hydrogenases of Entamoeba histolytica and Giardia lamblia: Activity of the Recombinant Entamoebic Enzyme ... (Site not responding. Last check: 2007-10-03)
		Iron-Dependent Hydrogenases of Entamoeba histolytica and Giardia lamblia: Activity of the Recombinant Entamoebic Enzyme and Evidence for Lateral Gene Transfer -- Nixon et al.
		Iron hydrogenases and the evolution of anaerobic eukaryotes.
		Evidence for an [Fe]-type hydrogenase in the parasitic protozoan Trichomonas vaginalis.
	www.biolbull.org /cgi/content/full/204/1/1 (3724 words)

	The membrane-bound hydrogenase from P. furiosus accepts electrons from ferredoxin
		The membrane-bound hydrogenase responsible for this activity was partially purified and characterized [1, my PhD thesis].
		Even at room temperature the membrane-bound hydrogenase is quite active, and redox titrations are hampered by the diversion of reducing equivalents to proton reduction.
		Sequence comparisons between several hydrogenases have allowed the description of specific signatures that can be used to selectively distinguish these membrane-bound hydrogenases from each other and from the very similar NADH: ubiquinone oxidoreductases.
	www2.ufp.pt /~pedros/science/mh2ase.htm (661 words)

	Pfam 21.0 : HycI (Site not responding. Last check: 2007-10-03)
		In E. coli HypI the hydrogenase maturation protease is involved in processing of HypE the large subunit of hydrogenases 3, by cleavage of its C-terminal [1].
		At one step of this process, after nickel incorporation, each hydrogenase isoenzyme is processed by proteolytic cleavage at the C-terminal end by the corresponding hydrogenase maturation endopeptidase PUBMED:10727938.
		For example, Escherichia coli HycI is involved in processing of pre-HycE (the large subunit of hydrogenase 3) PUBMED:8125094, PUBMED:10795682; HybD is involved in processing of pre-HybC (the large subunit of hydrogenase 2) PUBMED:10331925; and HyaD is assumed to be involved in processing of the large subunit of hydrogenase 1.
	pfam.janelia.org /cgi-bin/getdesc?name=HycI (629 words)

	Helicobacter hepaticus Hydrogenase Mutants Are Deficient in Hydrogen-Supported Amino Acid Uptake and in Causing Liver ... (Site not responding. Last check: 2007-10-03)
		Helicobacter hepaticus Hydrogenase Mutants Are Deficient in Hydrogen-Supported Amino Acid Uptake and in Causing Liver Lesions in A/J Mice -- Mehta et al.
		Helicobacter hepaticus Hydrogenase Mutants Are Deficient in Hydrogen-Supported Amino Acid Uptake and in Causing Liver Lesions in A/J Mice
		hepaticus hydrogenase is a hydrogen uptake hydrogenase that
	iai.asm.org /cgi/content/full/73/9/5311 (5427 words)

	Iron Hydrogenases and the Evolution of Anaerobic Eukaryotes -- Horner et al. 17 (11): 1695 -- Molecular Biology and ... (Site not responding. Last check: 2007-10-03)
		Iron Hydrogenases and the Evolution of Anaerobic Eukaryotes -- Horner et al.
		and TvhydB and the dimeric hydrogenase of Desulfovibrio sp.,
		Isolation, characterization and n-terminal amino-acid-sequence of hydrogenase from the green-alga Chlamydomonas reinhardtii.
	mbe.oxfordjournals.org /cgi/content/full/17/11/1695 (6630 words)

	Direct Solar Biohydrogen - GCEP
		The aim of this project is to engineer an organism in which a hydrogenase enzyme accepts those electrons from ferredoxin in order to generate hydrogen.
		This oxygen-sensitive catalytic center is buried in the center of the hydrogenase.
		The hydrogenase must be expressed and activated in the organism, the ferredoxin must be over-expressed, and an uncoupler protein must be produced to aid in the flow of protons.
	gcep.stanford.edu /research/factsheets/direct_solar.html (847 words)

	Stanford Scientific Magazine (Site not responding. Last check: 2007-10-03)
		Certain types of bacteria contain hydrogenases, enzymes that generate hydrogen from electron-transport chains used for metabolic processes.
		Once oxygen-tolerant hydrogenase enzymes are discovered, they can be reintroduced into the organisms from which they came, or transferred into new organisms.
		In this design, temperature would be controlled by circulating fluid in the lower layer, captured sunlight and bacteria would produce H2 in the middle layer, and gaseous waste would be pulled into a vacuum chamber in the upper layer.
	www.stanford.edu /~dgermain/volume4-2/articles4-2/hydrogen.html (869 words)

	Characterization of Hydrogenase II from the Hyperthermophilic Archaeon Pyrococcus furiosus and Assessment of Its Role ... (Site not responding. Last check: 2007-10-03)
		Characterization of Hydrogenase II from the Hyperthermophilic Archaeon Pyrococcus furiosus and Assessment of Its Role in Sulfur Reduction -- Ma et al.
		In contrast to hydrogenase I, hydrogenase II catalyzed the H
		The amino acid sequences of the four subunits of hydrogenase II showed between 55 and 63% similarity to those of hydrogenase I. The two enzymes are present in the cytoplasm at approximately
	jb.asm.org /cgi/content/abstract/182/7/1864 (476 words)

	CyanoNews Vol. 14, Number 1: ISPP - Hydrogenase (Site not responding. Last check: 2007-10-03)
		The discovery of reversible hydrogenase genes in Synechocystis PCC 6803 [Appel and Schulz (1996) Biochem Biophys Acta 1298:141-147] was followed by a proposal that the diaphorase part of the enzyme could be shared both by respiratory complex I and hydrogenase [Appel and Schulz; Schmitz and Bothe (1996) Naturwiss 83:525].
		In my opinion, the involvement of hydrogenase (as a whole or via one/more of its components) in either photosynthetic and/or respiratory electron transport is still an open question, as well as the biological significance of such an involvement.
		The reversible hydrogenase could serve as one more in a growing list of redox proteins involved in electron flow around PSI in Synechocystis PCC 6803 under normal or stressed conditions (light, anaerobiosis, salt, temperature, etc.), a topic abundently illustrated during the meeting.
	www.vcu.edu /cyanonews/V14/ispp-h2.htm (325 words)

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