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Topic: IC50

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	Innovadyne > Applications > IC50's > Nanodrop IC50 (Site not responding. Last check: 2007-10-21)
		Innovadyne’s technology is well suited to the performance of the dilution plates and the screens due to the hybrid syringe/solenoid aspirate and dispense architecture.
		IC50 screens can be run at low volumes using the solenoid-based, non-contact dispense component.
		Using the Nanodrop II it is possible to perform the required DMSO dilutions and follow the dilution protocol with screens utilizing the second plate position.
	www.innovadyne.com /confapp_ND_IC50.html (208 words)

	amfAR Clinical Research
		Ten of 14 patients had mutant virus at codon 215 detected by differential hybridization; AZT IC50 ratio significantly correlated with the proportion of mutant virus at codon 215 (r=0.68, p=0.0078).
		Nine of 10 patients had mutant virus detected at codon 70; a significant correlation between the proportion of mutant virus at codon 70 and AZT IC50 ratio was not seen.
		Overall, ddI IC50 was weakly correlated with the proportion of 215 mutant virus detected (r=0.61, p=0.02).
	www.amfar.org /cgi-bin/iowa/programs/researchc/record.html?record=60 (479 words)

	[No title]
		For example, the ATA procedure was used to determine the ammonia nitrogen IC50 between 7000 and 8000 mg/L for an anaerobic digester with a 25 or 40 day SRT, and ammonia nitrogen IC50 between 8000 and 9000 mg/L for an anaerobic digester with a 15-day SRT (Bhattacharya and Parkin, 1989).
		The detergent IC50 was 0.45% and the disinfectant IC50 was 0.25% (Nagel et al., 1999).
		The IC50 concentration for the mesophilic biomass was 0.7% as shown in Figure 13.
	www.eng.mu.edu /fileBroker.php/2477/Thermophilic_Anaerobic_Digestion_with_Ultrafiltration_for_Dairy_Manure.doc (12249 words)

	PMPA: New Resistance Data
		To the Q151M complex, although the mean increase in IC50 (reduced susceptibility or resistance) was 20 fold, a few viruses had between 4-10 fold or intermediate resistance.
		A mean 31 fold increased IC50 (reduced sensitivity) was displayed to AZT by viruses with the T69S and the M184V mutation.
		To abacavir, there was a 7.3 mean fold increase in IC50 to viruses with the L65R and the IC50 increased further to a mean 13 fold increase in IC50 when the M184V was present with the K65R.
	www.natap.org /2000/4thspain/rpt08spa_pmpa_new_062100.htm (1503 words)

	5-HT3A S225
		When this quintuple mutation was combined with hY228S and hV237I, there was a further decrement of 2-fold of the IC50 value of (+)-Tubocurarine relative to the quintuple mutant.
		This produced a 20-fold increase in IC50 relative to that observed for the WT m5-HT When this triplet mutation was also combined with mQ222Y and mK224R there was a 54-fold decrease in the IC50 of (+)-Tuocurarine relative to the mouse wild type.
		When this quintuple mutation was combined with mS233Y and mI242V, there was a further increment of 3-fold of the IC50 value of the (+)-Tubocurarine relative to the quintuple mutant.
	www.gwumc.edu /pharm/5-HT3/A_S225.htm (221 words)

	Meloxicam by drdoc on-line
		The IC50 represent the concentration required to produce 50% Inhibition of COX activity.
		Of note, the lower the IC50 Value for COX1 - the less the drug concentration required to inhibit COX1 and this is seen for example., with Piroxicam - where you have a high COX2 to COX1 ratio - suggesting a very low selectivity.
		The IC50 Studies are all in vitro studies and the ratios can vary according to the types of cell used and whether the tests are done instantaneously or on incubation and thus several different values for the same compound may be quoted depending of the biological system on which the tests were done.
	www.arthritis.co.za /melox.htm (1670 words)

	[No title]
		Concentrations of examined agents that induced decrease in 50% in cell survival (IC50), determined under the exactly same experimental conditions, are given in Table3.
		IC50 values have very good correlation with lipophylicity of studied compounds.
		IC50 - concentration of agent that induces a 50 % decrease in cell survival.
	www.chem.bg.ac.yu /~ijuranic/jscs02.htm (1778 words)

	Proanthocyanidines
		In the iron-promoted (Fenton-driven) model, procyanidines had a remarkable, dose-dependent antilipoperoxidant activity (IC50 = 2.5 mumol/l), more than one order of magnitude greater than that of the monomeric unit catechin (IC50 = 50 mumol/l), activity which is due, at least in part, to their metal-chelating properties.
		The HO degrees entrapping capacity of these compounds was further confirmed by UV studies and by electron spin resonance (ESR) spectroscopy, using DMPO as spin trapper: procyanidines markedly reduced, in a dose-dependent fashion, the signal intensity of the DMPO-OH radical spin adduct (100% inhibition at 40 mumol/l).
		In addition procyanidines non-competitively inhibit the activities of the proteolytic enzymes collagenase (IC50 = 38 mumol/l) and elastase (IC50 = 4.24 mumol/l) and of the glycosidases hyaluronidase and beta-glucuronidase (IC50 = 80 mumol/l and 1.1 mumol/l), involved in the turnover of the main structural components of the extravascular matrix collagen, elastin and hyaluronic acid.
	www.lef.org /prod_hp/abstracts/php-ab090.html (1466 words)

	Isoflavones - Vitacost (Site not responding. Last check: 2007-10-21)
		Genistein is a potent inhibitor of the growth of each cell line (IC50 values from 6.5 to 12.0 micrograms/ml), whereas biochanin A and daidzein are weaker growth inhibitors (IC50 values from 20 to 34 micrograms/ml).
		The presence of the estrogen receptor is not required for the isoflavones to inhibit tumor cell growth (MDA-468 vs MCF-7 cells).
		Growth inhibition by genistein was cytostatic and reversible at IC50 concentrations.
	www.vitacost.com /science/medstudies.cfm?litid=19 (3337 words)

	Synthesis and antiparasitic and antitumor activity of 2, 4-diamino-6-(arylmethyl)-5,6,7,8-tetrahydroquinazoline ...
		The three most potent inhibitors of P. carinii DHFR were the 2,5-dimethoxybenzyl (5j), 3, 4-dimethoxybenzyl (5k), and 3,4,5-trimethoxybenzyl (5l) analogues, with IC50 values of 0.057, 0.10, and 0.091 microM, respectively.
		Four compounds (5a,h,k,l) were tested against T. gondii tachyzoites in culture and were found to have a potency (IC50 = 0.1-0.5 microM) similar to that of pyrimethamine (IC50 = 0.69 microM), a standard clinical agent for the treatment of cerebral toxoplasmosis in AIDS patients.
		Among the 25 cell lines in the National Cancer Institute panel for which data were confirmed in two independent experiments, the IC50 for at least two of these compounds was <10 microM against 17 cell lines (68%) and in the 0.
	www.aegis.com /aidsline/1999/jun/A9960497.html (780 words)

	Assay for determining the IC50 of a drug! - Cell Biology
		I am confused that most of the researchers to investigate the IC50 of a particular drug will consider to use "serum free medium".
		After obtaining the IC50 concentration, they did not mention whether they were using serum free medium to perform other assay, such as Cell cycle assay...! I am concerning the issue that when to use serum free medium is suitable?
		But I did not use a cell cycle assay, I tested wether the receptor was activated, by measuring second messenger.
	www.protocol-online.org /archive/posts/3791.html (215 words)

	IC50 pss anaysis problem
		I guess it may be casued some Envirument Variable whichs is not set properly or maybe I need a OS fix for IC50 to run properly on RH80.
		However, I found out that spectreRF is not supported under ic50.
		IC50 can be installed under redhat7.2,redhat7.3&redhat8.0,but redhat9.0 can not be supported!
	www.edaboard.com /viewtopic.php?p=259982 (376 words)

	The Body: CNS Reservoirs
		This study proposes that clinicians consider using a ratio of the antiretrovirals cerebrospinal fluid (CSF) concentration to its inhibitory concentration for 50% of viruses (IC50).
		The authors examined the CSF concentrations (CSF conc) of all FDA-licensed ARVs and compared that to the IC50 ranges for each of those drugs, as determined by the Virologic Phenosense Assay (CSF conc/IC50).
		Based on these findings, the authors advocated the use of ARVs with high CSF conc/IC50 values and urged clinicians to consider phenotyping to determine the IC50 of HIV in the CSF.
	www.thebody.com /confs/retro2001/boyle5.html (608 words)

	HIV-1 ANTI-VIRAL DRUG STUDY: DDC IC50 VALUE (Site not responding. Last check: 2007-10-21)
		The invitro drug susceptibility measures the extent that a drug inhibits HIV p24 antigen production by PBMC acutely infected with viral isolates.
		HIV p24 antigen is quantified 50% inhibitory concentration (IC50) of drug is determined by calculating the concentration of drug necessary to produce a 50% reduction of standardized inoculum of the patient's HIV-1 isolate.
		"IC50 results of 5.0 mcM or greater denote resistance."
	www.mgh.org /lab/CATALOG/TESTS/2904.HTM (89 words)

	Design and models for estimating antagonist potency (pA2, Kd and IC50) following the detection of antagonism observed ... (Site not responding. Last check: 2007-10-21)
		Design and models for estimating antagonist potency (pA2, Kd and IC50) following the detection of antagonism observed in the presence of intrinsic activity.The anomalous MCCG response was also observed following treatment of the cells with MCCG in the absence of added agonist.
		With their use, the data acquired in both assays support the designation of MCCG as a competitive antagonist of the hmGlu2 receptor and provide similar pA2 estimates between assays.
		We derived an alternate design and models that permit estimation of the pA2 (pAx), Kd and IC50 for antagonists which produce a concentration related effect when applied by themselves.
	www.pdg.cnb.uam.es /UniPub/iHOP/gp/1862277.html (325 words)

	Measure the concentration-response and IC50 of a drug on different cardiac ion channels
		The Patch Clamp concentration-response relationship (C-R curve) is the best quantitative measurement of drug block of ion channels - it is considered the Gold Standard assay.
		The IC50 derived from the C-R curve may be used to determine safety margins and therapeutic doses.
		The ICH S7b guidelines specify that the hERG IC50 assay be conducted for all drugs under consideration for an IND.
	www.chantest.com /ic50.html (338 words)

	873140 Exhibits Potent Antiviral Activity Against a Broad Panel of HIV-1 Envelopes From Treatment Naive and Experienced ...
		Median IC50 for R5-tropic (n=338) samples was 6.81 nM (range: 0.89-33.19).
		The CCR5-using components of R5X4-tropic (n=64) samples were susceptible with a median IC50 of 3.61 nM (0.67-14.72).
		Susceptibility (IC50 (nM)) of HIV envelopes in plasma (N=402) to 873140 was evaluated by the ViroLogic PhenoSense HIV Entry assay _ --This assay uses an engineered cell line that expresses CD4 and only CCR5
	www.natap.org /2005/ias/ias_36.htm (707 words)

	IC50's - Innovadyne
		IC50 assays (assays to determine the concentration of a drug-like compound resulting in a 50% reduction in activity of a disease target) are a commonly used method for assessing drug efficacy in pharmaceutical screening regimens.
		Typically, these assays are performed via serial dilutions of DMSO compound libraries to achieve dilutions of 2x10
		Subsequent to the DMSO serial dilution, assays are performed with each dilution to ascertain the IC50 of the compound of interest.
	www.innovadyne.com /apps_IC50.html (236 words)

	IC50
		IC50 assays are a commonly used method for assessing drug efficacy in pharmaceutical screening regimens.
		Typically, these assays are performed via serial dilutions of DMSO compound libraries to achieve dilutions of 2x107 in 100% DMSO.
		Related Terms: DMSO serial dilution, IC50 serial dilution, serial dilution
	www.innovadyne.com /_IC50.html (258 words)

	165_Lipoxygenases Inhibitors
		This compound is a selective inhibitor of 12-lipoxygenase(2) (IC50 = 0.12 µM for platelet type 12-LOX).
		C19H15NO4 is a general lipoxygenase inhibitor is more potent for 12-lipoxygenase (IC50 = 63 nM) than 5-lipoxygenase (IC50 = 1.89 µM) or 15-lipoxygenase (IC50 = 3.33 µM).
		This compound selectively inhibits lipoxygenases [5-lipoxygenase (IC50 = 0.2 µM), 12-lipoxygenase (IC50 = 30 µM), 15-lipoxygenase (IC50 = 0.3 µM)] compared to cyclooxygenase (4, 5, 6) (IC50 = 100 µM.).
	www.oxfordbiomed.com /lox165inhib.html (246 words)

	Clinical Study: 01-I-0004, An Assessment of the Relationship Between Antiretroviral Drug Genotype/Phenotype (IC50) and ...
		Clinical Study: 01-I-0004, An Assessment of the Relationship Between Antiretroviral Drug Genotype/Phenotype (IC50) and Antiretroviral Activity in HIV-Infected, Drug-Experienced Patients with Suboptimal Suppression of Plasma Viral Load
		An Assessment of the Relationship Between Antiretroviral Drug Genotype/Phenotype (IC50) and Antiretroviral Activity in HIV-Infected, Drug-Experienced Patients with Suboptimal Suppression of Plasma Viral Load
		The purpose of this study is to determine how laboratory tests called genotyping and phenotyping assess the effectiveness of antiretroviral drugs used to treat HIV infection.
	clinicalstudies.info.nih.gov /detail/A_2001-I-0004.html (1267 words)

	Research (Site not responding. Last check: 2007-10-21)
		The COX-1 and COX-2 activities were determined based upon the PGE2 levels generated during the incubations of human platelets with tested compounds and/or vehicle by the Amersham ELA assay[132].
		The IC50 of TNJ, Aspirin, Indomethacin, and Celebrex on COX-1 are 5 %, 4.55 mmol/L, 0.01 mmol/L, and 1.4 mmol/L, respectively, and that for COX-2 are 3.8 %, 595 mmol/L, 0.4 mmol/L, and 0.47 mmol/L respectively.
		The data was converted into a ratio of IC50 COX-2/COX-1.
	www.noni-4u.com /research.htm (7903 words)

	Ligand specificity of proteases and Kinases: an applicationto IC50 prediction on a large scale (Site not responding. Last check: 2007-10-21)
		Using thousands of activity data in ProLINT, we have developed a unified model to predict inhibition coefficient (IC50) for a choice of ligand and protein.
		Each of the protease and kinase included in the database has been assigned a consensus ligand fragment signature, based on the available information about its interaction with different ligands.
		Results of predictions are promising and compared well with existing QSAR models for specific proteins.
	www.iscb.org /ismb2003/posters/saraiATbse.kyutech.ac.jp_274.html (225 words)

	TAG: Position Paper on atazanavir (BMS-232623), brand name Reyataz®
		The inhibitory quotient is a model for understanding and evaluating drug pharmacology, including both pharmacokinetics and the potential development of resistance.
		IQ is the ratio between the minimum drug concentration (Cmin, the "trough") and the 50% or 90% inhibitory concentration (IC50 or IC90).
		Inhibitory quotient of protease inhibitors using a standardized determination of IC50.
	www.aidsinfonyc.org /tag/tx/atazanavir.html (4357 words)

	ASCO - Abstracts - PubMed Abstract View (Site not responding. Last check: 2007-10-21)
		(-)-Rolipram (IC50 = 0.22 +/- 0.08 microM) was 2.5-fold more potent than (+)-rolipram (IC50 = 0.58 +/- 0.05 microM) in inhibiting membrane-bound PDE IV.
		Solubilization of PDE IV with deoxycholate (0.5%) and NaCl (100 mM) increased rolipram stereospecificity [IC50 (-)-rolipram = 0.020 +/- 0.002 microM; IC50 (+)-rolipram = 0.33 +/- 0.07 microM].
		Vanadate-glutathione complex (V-GSH) stimulated membrane-bound PDE IV activity and increased the potency of (-)-rolipram (IC50 = 0.014 +/- 0.006 microM) but not (+)-rolipram (IC50 = 0.32 +/- 0.07 microM).
	www.asco.org /ac/1,1003,_12-002867-00_18-008387267,00.asp?SearchNames= (387 words)

	ASCO - Browse by Meeting - Effect of C-erbB-2 and Topoisomerase II-Alpha on Chemosensitivity of Native Ovarian ... (Site not responding. Last check: 2007-10-21)
		Population-based cumulative dose-response curves for both IC50 and IC90 were calculated for each drug, the area under the curves (AUC) were used for quantitave comparison of chemosensitivity with high values indicating high sensitivity.
		Regarding CDDP (IC50,IC90) and PCT (IC50) the c-erbB-2+ were more chemosensitive than the c-erbB-2- tumors.
		TopoII-alpha+ tumors tended to be more chemosensitive towards ANT, CDDP (IC50) and PCT (IC50,IC90) than the topoII-alpha- tumors.
	www.asco.org /ac/1,1003,_12-002702-00_18-0010-00_19-002474,00.asp (847 words)

	AEGiS-14IAC: Tipranavir/ritonavir: derivation of doses that achieve effective plasma concentrations above the IC50 for ...
		AEGiS-14IAC: Tipranavir/ritonavir: derivation of doses that achieve effective plasma concentrations above the IC50 for multiple PI-resistant HIV-1 viruses.
		Tipranavir/ritonavir: derivation of doses that achieve effective plasma concentrations above the IC50 for multiple PI-resistant HIV-1 viruses.
		The mean IC50 increase for this virus panel was: TPV 2-fold, indinavir 44-fold, nelfinavir 45-fold, saquinavir 46-fold, and ritonavir 87-fold (Larder, AIDS, 2000;14(13):1943-8).
	www.aegis.com /conferences/iac/2002/TuPeB4437.html (421 words)

	NDST2 - N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (Site not responding. Last check: 2007-10-21)
		Reverse transcription polymerase chain reaction analysis revealed more restricted tissue expression of hNDST3 than hNDST1 and NDST2, and high levels in brain, liver, and kidney.
		[(19)F]FP-Gluc-TOCA showed no affinity to hsst1 and hsst3, moderate affinity to hsst4 (IC(50): 437+/-84 n M) and hsst5 (IC(50): 123+/-8.8 n M) and very high affinity to hsst2 (IC(50): 2.8+/-0.4 n M).
		Appropriate N-terminus modification can result in somatostatin (SRIF) octapeptide analogs that are both more potent and more selective in vitro for the human SRIF receptor type 2 (hsst2).
	www.ihop-net.org /UniPub/iHOP/gg/93905.html (724 words)

	N-[2-(1-cyclohexenyl)ethyl]-N'-[2-(5-bromopyridyl)]-thiourea and ...
		HI-346 and HI-445 effectively inhibited recombinant RT with better IC50 values than other anti-HIV agents tested.
		The ranking order of efficacy in cell-free RT inhibition assays was: HI-346 (IC50 = 0.4 microM) > HI-445 (IC50 = 0.5 microM) > trovirdine (IC50 = 0.8 microM) > MKC-442 (IC5 = 0.8 microM) = delavirdine (IC50 = 1.5 microM) > nevirapine (IC50 = 23 microM).
		In accord with this data, both compounds inhibited the replication of the drug-sensitive HIV-1 strain HTLV(IIIB) with better IC50 values than other anti-HIV
	www.aegis.com /aidsline/2000/jan/A0010887.html (350 words)

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