
	Where results make sense

Topic: Immunofluorescence

Related Topics

Viral plaque

Cell culture

VDRL

PVDF

PCR

Microbiology

Syphilis

Treponema pallidum

In the News (Mon 6 Jul 09)

Northern Trust

Marvin Harrison

Peter Chernin

Gary Locke

Match Play

Penelope Cruz

Mickey Rourke

AR Rahman

Danny Boyle

Hugh Jackman

	MedlinePlus Medical Encyclopedia: Immunofluorescence (Site not responding. Last check: 2007-11-05)
		Immunofluorescence is a laboratory technique to identify specific antibodies or antigens.
		This test is used to detect and roughly measure the amount of antibodies in the blood.
		Immunofluorescence is often used to detect the presence and amount of antinuclear antibody (ANA).
	www.nlm.nih.gov /medlineplus/ency/article/003521.htm (717 words)

	Dang, Mol Vis 2004; 10:323-327.
		Results: Punctate immunofluorescence, indicating the presence of gap junctions, was observed in the inner and outer plexiform layers of both wild type and cone-less and rod-less retinas.
		Immunofluorescence analysis of connexin 36 in fixed retinas using a connexin 36 specific antibody showed that the expression of the protein is greatly increased in the OPL of cone-only retina in comparison to the control and that it is marginally lower in the absence of cones.
		Densitometric analysis of the immunofluorescence, shown in Table 1, revealed the OPL of the cone-only retina to be 2050% of that of the wild type.
	www.molvis.org /molvis/v10/a41 (2900 words)

	Skin-Immunofluorescence
		Indirect immunofluorescence was performed on skin from 13 patients with epidermolysis bullosa (EB) (simplex, 6; junctional, 2; dystrophic, 5) to compare the expression of 3 basement membrane components, bullous pemphigoid (BP) antigen, cicatricial pemphigoid (CP) antigen, and epidermolysis bullosa acquisita (EBA) antigen, in clinically uninvolved tissue.
		Immunofluorescence microscopy of the skin has disclosed antibodies bound to epidermal cell nuclei in several connective tissue disorders.
		To establish the diagnostic potential of this phenomenon the results of immunofluorescence microscopy of biopsy specimens from 1651 subjects with various diseases and from 315 patients with systemic connective tissue disorders and related diseases were reviewed.
	www.thedoctorsdoctor.com /bodysites/skin_immunofluorescence.htm (3643 words)

	* Immunofluorescence - (Disease): Definition (Site not responding. Last check: 2007-11-05)
		Immunofluorescence is often used to detect and quantitate (ANA).
		IMMUNOFLUORESCENCE ASSAY (IFA) This method is sometimes used to confirm ELISA results instead of Western blotting.
		Immunofluorescence tests, where special viral markers react with human antibodies that have been tagged with a fluorescent chemical, are used to verify the disease...
	www.bestknows.com /disease/immunofluorescence.html (254 words)

	Biological tissue -- Facts, Info, and Encyclopedia article (Site not responding. Last check: 2007-11-05)
		The study of tissues is known as (The branch of biology that studies the microscopic structure of animal or plant tissues) histology, or, in connection with disease, (additional info and facts about histopathology) histopathology.
		The classical tools for studying the tissues are the wax block, the tissue stain, and the (additional info and facts about optical microscope) optical microscope, though developments in (Microscopy with the use of electron microscopes) electron microscopy, immunofluorescence, and frozen sections have all added to the sum of knowledge in the last couple of decades.
		With these tools, the classical appearances of the tissues can be examined in health and disease, enabling considerable refinement of clinical diagnosis and prognosis.
	www.absoluteastronomy.com /encyclopedia/b/bi/biological_tissue.htm (279 words)

	CDC - Immunofluorescence Assay for Serologic Diagnosis of SARS
		CDC - Immunofluorescence Assay for Serologic Diagnosis of SARS
		We conducted this study at the teaching hospital of the Chinese University of Hong Kong, Prince of Wales Hospital, where a major outbreak of SARS had occurred (9).
		The immunofluorescence test can also be used as a confirmatory assay for samples reactive to screening assays.
	www.cdc.gov /ncidod/EID/vol10no3/03-0493.htm (1845 words)

	Immunofluorescence Labeling Method (Site not responding. Last check: 2007-11-05)
		The purpose of immunofluorescence is to detect the location and relative abundance of any protein for which you have an antibody.
		This immunofluorescence micrograph shows the ER being labeled with a monoclonal antibody against the the chicken calcium ATPase.
		Using immunofluorescence, investigators can see when, where and how much of their favorite protein is expressed in any cell or tissue.
	occawlonline.pearsoned.com /bookbind/pubbooks/bc_mcampbell_genomics_1/medialib/method/IMF.html (460 words)

	Immunofluorescence (Site not responding. Last check: 2007-11-05)
		In direct immunofluorescence, a specific antibody (against the antigen or protein of interest, whose introduction into thehost animal generates host-derived antibodies) is labeled by chemically attaching a fluorochrome.
		The conjugate is spread on a slide containing the suspected presence of the particular antigen (typically an extract of cells cultured on a solid biomaterial substrate).
		The presence of the chemically attached fluorescent conjugate and the antigen is demonstrated when the fluorochrome is excited at its known excitation peak and the emission intensities are captured visually or by a camera.
	www.rci.rutgers.edu /~moghe/PVM99LEC3/tsld017.htm (252 words)

	Encyclopedia: Immunofluorescence (Site not responding. Last check: 2007-11-05)
		Immunofluorescently labelled tissue sections are studied using a fluorescence microscope or by confocal microscopy.
		Schematic of antibody binding to an antigen An antibody is a protein used by the immune system to identify and neutralize foreign objects like bacteria and viruses.
		Immunohistochemistry - In Situ Hybridization Immunofluorescence: Staining Protocols, Image Gallery, Discussion, News and Articles.
	www.nationmaster.com /encyclopedia/Immunofluorescence (216 words)

	Immunology/Immunofluorescence Protocols
		Overview: Immunofluorescence is a technique allowing the visualization of a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye such as fluorescein isothiocyanate (FITC).
		There are two major types of immunofluorescence staining methods: 1) direct immunofluorescence staining in which the primary antibody is labeled with fluorescence dye, and 2) indirect immunofluorescence staining in which a secondary antibody labeled with fluorochrome is used to recognize a primary antibody.
		Immunofluorescence stained samples are examined under a fluorescence microscope or confocal microscope.
	www.protocol-online.org /prot/Immunology/Immunofluorescence (398 words)

	Phycologia: Use of immunofluorescence microscopy to compare small nuclei in two populations of Caulerpa taxifolia ... (Site not responding. Last check: 2007-11-05)
		Using immunofluorescent microscopy, it was possible to recognize nuclei and chromosomes in dividing nuclei.
		For immunofluorescence labeling, cytoplasm was fixed for 15 min in 4% formaldehyde and 2% glutaraldehyde in phosphate buffer.
		Abundant nuclei were observed in cytoplasm from the stolons and fronds of C. taxifolia prepared by DAPI staining and by immunofluorescent labeling.
	www.findarticles.com /p/articles/mi_qa3850/is_199807/ai_n8783540 (1344 words)

	In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice -- Sharma and Shimeld 81 (9): 785 -- ...
		In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice -- Sharma and Shimeld 81 (9): 785 -- British Journal of Ophthalmology
		Direct immunofluorescence was used in vivo in a masked study to diagnose HSK in mice using a standard slit lamp with cobalt
		Immunofluorescence staining techniques are used routinely in vitro to diagnose infections especially viruses including HSV.
	bjo.bmjjournals.com /cgi/content/full/81/9/785 (2149 words)

	Biopsy coupled to quantitative immunofluorescence: a new method to study the human vascular endothelium -- Colombo et ...
		Quantitative immunofluorescence and immunoblot analysis of protein expression in cultured endothelial cells.
		Quantitative immunofluorescence and immunoblot analysis of cellular proteins were compared in cultured endothelial cells (Fig.
		Immunofluorescence analysis of protein expression in cultured endothelial cells was performed in duplicate and measurements
	jap.physiology.org /cgi/content/full/92/3/1331 (3874 words)

	Immunofluorescence of the Cytoskeleton
		Some cellular structures may get dislodged by the borohydride although this method has been used successfully for microtubule immunofluorescence in neurons which tend to be fairly fragile.
		This colocalization of motors with microtubules is abolished by addition of ATP to the extraction buffer suggesting that the observed colocalization is artifactually generated by rigor binding of motors to microtubules during the extraction.)
		The optimal condition for the antibody is then used for double label immunofluorescence with microtubules.
	mitchison.med.harvard.edu /protocols/gen1.html (2144 words)

	Live-cell immunofluorescence for detection of human immunodeficiency virus type 1 (HIV-1)-related antigens in ...
		Live-cell indirect immunofluorescence was used to detect HIV-associated antigens in fresh uncultured peripheral blood mononuclear cells from 29 HIV-seropositive subjects.
		Conventional fixed-cell immunofluorescence and live-cell immunofluorescence were run in parallel on each sample using monoclonal antibodies directed against HIV gag and env gene products.
		Results strongly indicate that nondenaturing live-cell immunofluorescence is much more sensitive for detection of HIV-associated antigens and may be useful in the diagnosis of HIV infection as well as in the study of pathogenesis.
	www.aegis.com /aidsline/1990/mar/M9030347.html (376 words)

	Phosphorylation-induced Rearrangement of the Histone H3 NH2-terminal Domain during Mitotic Chromosome Condensation -- ...
		and the H3 Ab immunofluorescence was correspondingly weaker (Fig.
		SPM8-2 antibody immunofluorescence shows that the predominantly nuclear localization of spermine and spermidine in cells arrested in mitosis with nocodazole (A) is lost after 15 min of staurosporine treatment (B).
		Weak speckles of H3P Ab immunofluorescence were also observed in the nuclei of all interphase cells.
	www.jcb.org /cgi/content/full/145/2/225 (7071 words)

	Immunofluorescence Staining & Flow Cytometry of Cell Surface Antigens
		Immunofluorescence Staining and Flow Cytometry of Cell Surface Antigens
		Start by determining the best source of cells and their viability by carefully titrating reagents in preliminary experiments to find the best antibody combinations/concentrations and incubation conditions, and by including all the appropriate controls.
		Protocol A: Immunofluorescence Staining of Cell Suspensions of Murine Lymphoid Tissue
	www.ebioscience.com /ebioscience/appls/FCS.htm (1053 words)

	Comparison of a New Neuraminidase Detection Assay with an Enzyme Immunoassay, Immunofluorescence, and Culture for Rapid ...
		Comparison of a New Neuraminidase Detection Assay with an Enzyme Immunoassay, Immunofluorescence, and Culture for Rapid Detection of Influenza A and B Viruses in Nasal Wash Specimens -- Noyola et al.
		Comparison of a New Neuraminidase Detection Assay with an Enzyme Immunoassay, Immunofluorescence, and Culture for Rapid Detection of Influenza A and B Viruses in Nasal Wash Specimens
		Comparison of rapid immunofluorescence procedure with Testpack RSV and Directigen FLU-A for diagnosis of respiratory syncytial virus and influenza A virus.
	jcm.asm.org /cgi/content/full/38/3/1161 (3771 words)

	Immunofluorescence Labeling of Calcium Pump (Site not responding. Last check: 2007-11-05)
		Immunofluorescence was used to lable the chicken calcium ATPase.
		Photomicrographs of an embryonic chicken heart in bright field (left) and immunofluorescence (right) illumination.
		Since color film was used, the secondary antibody appears green instead of white in the photograph.
	occawlonline.pearsoned.com /bookbind/pubbooks/bc_mcampbell_genomics_1/medialib/method/IMF2.html (274 words)

	Antibody Testing for Chlamydia psittaci using a rapid ELISA-KIT (Site not responding. Last check: 2007-11-05)
		The Immunocomb (IC) ELISA test and immunofluorescence (IF) microscopy were compared on their ability to diagnose Chlamydia psittaci infection.
		The blood samples were collected by clipping the bird's toe nail and allowing drops of blood to saturate the pre-punched filter paper disks that are provided in the Immunocomb kit.
		The validity of sensitivity and specificity calculations must be evaluated in light of the fact that the IC and the IF techniques are testing different pathophysiologic parameters of infection which are not always present at the same time.
	www.vet.uga.edu /IVCVM/1998/bendheim2/bendheim2.htm (1044 words)

	Tyramide signal amplification method in multiple-label immunofluorescence confocal microscopy.
		The TSA method, when properly optimized to individual tissues and primary antibodies, is an important tool for immunofluorescence microscopy.
		Furthermore, the TSA method and enzyme pretreatment can be complementary to achieve a high detection sensitivity, particularly in formalin-fixed paraffin-embedded archival tissues.
		Using multiple-label immunofluorescence confocal microscopy to characterize the cellular localization of antigens, the TSA method can be critical for double labeling with unconjugated primary antibodies raised in the same host species.
	www.aegis.com /aidsline/2000/jan/A0010929.html (324 words)

	BD Biosciences : Pharmingen : Protocols : Cell Staining for Immunofluorescence Microscopy
		If it is desirable to examine the co-distribution of two different antigens in the same cell, a double immunofluorescence procedure may be used.
		Cells may be incubated simultaneously with two primary antibodies, provided they are monospecific and can be distinguished with secondary antibodies conjugated to different fluorochromes (or with primary antibodies directly conjugated to different fluorochromes).
		High-quality conjugated antibodies are essential for the avoidance of cross-reactivity between two different antibodies in double immunofluorescence protocols.
	www.bdbiosciences.com /pharmingen/protocols/Immunofluorescence_Microscopy.shtml (598 words)

	Importance of anticomplement immunofluorescence antibody titration for diagnosing varicella-zoster virus infection in ... (Site not responding. Last check: 2007-11-05)
		Importance of anticomplement immunofluorescence antibody titration for diagnosing varicella-zoster virus infection in Bell's palsy -- Shigeta et al.
		Importance of anticomplement immunofluorescence antibody titration for diagnosing varicella-zoster virus infection in Bell's palsy
		Anticomplement Immunofluorescence was used for antibody titration against
	jcp.bmjjournals.com /cgi/content/abstract/39/11/1254 (234 words)

	Diseases for which Immunodermatology tests are indicated
		Skin biopsy for direct immunofluorescence and serum for indirect immunofluorescence (basement membrane zone).
		Skin biopsy for direct immunofluorescence; for bullous lupus erythematosus, also serum for indirect immunofluorescence (basement membrane zone).
		Skin biopsy for direct immunofluorescence and serum for indirect immunofluorescence (cell surface).
	www.med.utah.edu /derm/immunoderm/disease.cfm (226 words)

	Immunofluorescence stimulated emission depletion microscopy - Nature Biotechnology (Site not responding. Last check: 2007-11-05)
		Immunofluorescence stimulated emission depletion microscopy - Nature Biotechnology
		We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit.
		We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation.
	www.nature.com /doifinder/10.1038/nbt897 (128 words)

	Immunofluorescence in group B streptococcal infection and idiopathic respiratory distress syndrome -- Pinnas et al. 63 ...
		Immunofluorescence in group B streptococcal infection and idiopathic respiratory distress syndrome -- Pinnas et al.
		Articles by Pinnas, J. Articles by Fenton, L. Immunofluorescence in group B streptococcal infection and idiopathic respiratory distress syndrome
		Immunofluorescence was performed on lung tissue obtained at necropsy from
	pediatrics.aappublications.org /cgi/content/abstract/63/4/557 (245 words)

	Advanced Biotechnologies Inc Online: Products: Immunofluorescence and ELISA Diagnostic Kits for Virus Detection, ...
		All ABI research use kits are supplied on cold packs.
		Minimum shelf life at time of shipment will be no less than 3 months.
		Immunofluorescence and ELISA kits are currently available in IgG format.
	www.abionline.com /products/diagnostic/diagnosticFAQ.htm (222 words)

Try your search on: Qwika (all wikis)