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Topic: Immunohistochemistry

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	Immunohistochemistry
		Immunohistochemistry is an important application of monoclonal as well as polyclonal antibodies to determine the tissue distribution of an antigen of interest in health and disease.
		Immunohistochemistry is widely used for diagnosis of cancers, because specific tumor antigens are expressed de novo or upregulated in certain cancers.
		Immunohistochemistry requires the availability of biopsies, these are processed into sections with a microtome, then the sections are incubated with an appropriate antibody.
	www.antibody.com /IH.html (365 words)

	IMMUNOHISTOCHEMISTRY IN CNS
		Immunohistochemistry is an amalgamation of immunology and histology.
		The vast progress in the field of immunohistochemistry along with the knowledge of cell and molecular biology allows the exploration of the molecular phenotypes of the developing CNS tumors.
		The future of immunohistochemistry is aimed at not only the diagnosis and prognostication of the tumors but also being able to comment upon the probable response to various chemotherapeutic agents.
	www.thamburaj.com /immunohistochemistry.htm (1972 words)

	National Diagnostics - Articles - Advanced Histological Technique - Immunohistochemistry (Site not responding. Last check: 2007-11-05)
		Immunohistochemistry is generally carried out in sectioned tissue, which allows the antibodies free access to the interior of the cells.
		Immunohistochemistry can also be carried out on cells either in free solution or bound to membranes, or on monolayers of cultured cells.
		Intracellular Immunohistochemistry requires that the antibody to the target antigen be able to penetrate the cell membrane and whatever cell wall may be present before it can attach to the antigen.
	nationaldiagnostics.com /article_info.php/articles_id/108 (598 words)

	Immunohistochemistry Resources
		Techniques is a timely overview of antigen and nucleic acid retrieval methods currently in use or under investigation in the practice of immunohistochemistry...
		Immunohistochemistry in a variety of methods including immunogold;...
		Immunohistochemistry covers a well established series of techniques that, by...
	www.hoslink.com /immunohis.htm (569 words)

	Immunohistochemistry and Quantitative Analysis of Protein Expression Archives of Pathology & Laboratory Medicine - ...
		However, the inherent subjectivity of the assessment of an objective value (the in situ protein concentration) suggests that new technologies to measure the protein concentration may be required to achieve the accuracy required for companion diagnostics.
		Conclusions.-Although there is a long history of efforts to quantify immunohistochemistry, there has been a lack of broad acceptance because the resultant objective accuracy has not significantly improved outcome measures compared with the traditional, conventional analysis by eye.
		Immunohistochemistry has become a standard assay in surgical pathology, despite the fact that it often lacks reproducibility and standardization.
	www.findarticles.com /p/articles/mi_qa3725/is_200607/ai_n16598084 (748 words)

	BioMed Central \| Full text \| Combined immunohistochemistry of beta-catenin, cytokeratin 7, and cytokeratin 20 is useful ...
		The purpose of this study was to evaluate the clinical usefulness of immunohistochemistry of β-catenin, cytokeratin (CK) 7, and CK20 for the discriminating diagnosis of lung cancer.
		It has been reported that CK is distributed in a tissue-specific manner and that immunohistochemistry of certain CKs is useful for the determination of tissues of origin in several types of tumors [10].
		Furthermore, accumulation of β-catenin in the nucleus and/or cytoplasm was detected by immunohistochemistry in 80%–90% of colorectal cancer cases [8,15,16].
	www.biomedcentral.com /1471-2407/6/31 (3552 words)

	Immunohistochemistry: A Primer
		Immunohistochemistry (IHC) uses immunologic and histologic techniques to detect antigens in tissues. The antigen is recognized by a specific antibody (Fig.
		In summary, immunohistochemistry is a valuable technique for the diagnosis of infectious and neoplastic diseases of animals. It is sensitive, specific, economical and relatively easy to perform. Although not always considered the"gold standard", it can be as specific as bacterial and virus isolation, provided adequate controls are used.
		Immunohistochemistry : Detects antigens or sequences of antigens (4-10 amino acids) that are characteristic of an infectious agent or a type of cell.
	www.addl.purdue.edu /newsletters/2004/Fall/ihcprim.htm (438 words)

	Routine Syndecan-1 Immunohistochemistry Aids in the Diagnosis of Chronic Endometritis Archives of Pathology & ... (Site not responding. Last check: 2007-11-05)
		Immunohistochemistry stains for this antibody may facilitate diagnosis of chronic endometritis.
		Previously, we reported that the antibody to syndecan-1 was expressed on plasma cells and was a useful adjunct in the diagnosis of chronic endometritis.3 In order to test the feasibility of this conclusion, we prospectively performed immunohistochemistry for syndecan-1 on all endometrial curettage specimens with a non-neoplastic diagnosis and also attempted to obtain clinical follow-up.
		Our hypothesis was that syndecan-1 immunohistochemistry would increase the sensitivity of plasma cell detection, thus aiding in the diagnosis of chronic endometritis.
	www.findarticles.com /p/articles/mi_qa3725/is_200409/ai_n9441062 (709 words)

	The role of immunohistochemistry in Dermatopathology
		Immunohistochemistry has become an important tool in the diagnosis of the vast range of neoplastic and non-neoplastic diseases in dermatopathology.
		- Role of immunohistochemistry in the diagnosis of problematic adnexal and epidermal tumours.
		Role of immunohistochemistry in the diagnosis of problematic adnexal and epidermal tumou
	www.histopathology-india.net /Immuno.htm (1771 words)

	Using immunohistochemistry to study plant metabolism: the examples of its use in the localization of amino acids in ... (Site not responding. Last check: 2007-11-05)
		Using immunohistochemistry to study plant metabolism: the examples of its use in the localization of amino acids in plant tissues, and of phosphoenolpyruvate carboxykinase and its possible role in pH regulation -- Walker et al.
		Using immunohistochemistry to study plant metabolism: the examples of its use in the localization of amino acids in plant tissues, and of phosphoenolpyruvate carboxykinase and its possible role in pH regulation
		immunohistochemistry in which the antigen is known to be abundant.
	jxb.oxfordjournals.org /cgi/content/full/52/356/565 (6728 words)

	Immunohistochemistry - In Situ Hybridization (Site not responding. Last check: 2007-11-05)
		immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ).
		Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.
		This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis.
	www.immunoportal.com (1597 words)

	Immunohistochemistry - In Situ Hybridization Site Map
		Immunohistochemistry in the Differential Diagnosis of Schwannoma and Neurofibrom
		Immunohistochemistry vade mecum - An excellent piece of work by Dr. Paul W. Bishop You can search for tumours to find out their pattern of immunoreactivity or look for individual antibodies to check their specification.
		Co-Localization of Multiple Antigens and Specific DNA - Co-localization of proteins and nucleic acid sequences by in situ hybridization and immunohistochemistry is frequently difficult as the process necessary to detect the target structure of one technique may negatively affect the target of the other.
	www.immunoportal.com /immunohistochemistry.html (6339 words)

	DRAFT GUIDANCE FOR SUBMISSION OF IMMUNOHISTOCHEMISTRY APPLICATIONS TO THE FDA
		Immunohistochemistry tests systems (IHC’s) are in-vitro diagnostic devices consisting of polyclonal or monoclonal antibodies labeled with directions for use and performance claims, which may be packaged with ancillary reagents in kits.
		Immunohistochemistry devices (IHC) are subject to FDA regulation as in vitro diagnostic medical devices.
		Immunohistochemistry is a multistep diagnostic process that consists of specialized training in the selection of the appropriate reagents; tissue selection, fixation, and processing; preparation of the IHC slide; and interpretation of the staining results.
	www.fda.gov /cdrh/ode/immuno.html (11307 words)

	LifeSpan Biosciences - Immunohistochemistry Studies
		Immunohistochemistry is an important method for validating observations made by using nucleic acid based expression analysis, such as RT-PCR or gene-chip array technologies.
		A protein's normal expression profile can be understood by using immunohistochemistry to screen broad panels of normal tissues.
		Immunohistochemistry coupled with multiple full size cancer specimens of various stages is useful in studying these expression variations.
	www.lsbio.com /products/contracts (1493 words)

	Immunohistochemistry and Immunocytochemistry - Introduction to Antibodies
		Detection of antigens in tissues is known as immunohistochemistry, while detection in cultured cells is generally termed immunocytochemistry.
		For immunohistochemistry, the common options are fixed or unfixed cryostat (frozen) sections, fixed “wet” or vibritome sections, or fixed, paraffin-embedded sections.
		Because CN is soluble in alcohol and other organic solvents, the slides must not be dehydrated, exposed to alcoholic counterstains, or coverslipped with mounting media containing organic solvents.
	www.chemicon.com /resource/ANT101/a2D.asp (4686 words)

	The Society for Applied Immunohistochemistry
		Registration data is the property of The Society for Applied Immunohistochemistry, subject to and protected by the Electronic Communications Privacy Act.
		The Society for Applied Immunohistochemistry uses SSL (Secure Socket Layer) technology to encrypt data transmitted during the registration process.
		Although The Society for Applied Immunohistochemistry will use all reasonable efforts to safeguard the confidentiality of the Registration Data, transmissions made by means of the Internet cannot be made absolutely secure.
	www.appliedimmuno.org /privacyPolicy.html (577 words)

	Appendix - Introduction to Antibodies
		Antigen retrieval in immunohistochemistry under the influence of pH using monoclonal antibodies.
		Immunohistochemistry (IMM-yu-no-HIST-oh-KEM-eh-stree) — A technique that uses antibodies to probe specific antigens in fresh frozen or processed tissue.
		Immunoprecipitation (IMM-yu-no-PREE-sip-eh-tay-shun) — A technique that uses antibodies to bind antigens in solution to yield a precipitating complex, the components of which can then be isolated.
	www.chemicon.com /resource/ANT101/a3.asp (1427 words)

	Technical variations in prostatic immunohistochemistry: need for standardisation and stringent quality assurance in PSA ...
		Technical variations in prostatic immunohistochemistry: need for standardisation and stringent quality assurance in PSA and PSAP immunostaining -- Varma et al.
		Technical variations in prostatic immunohistochemistry: need for standardisation and stringent quality assurance in PSA and PSAP immunostaining
		The questionnaire was circulated to 394 laboratories, 323 of
	jcp.bmjjournals.com /cgi/content/full/57/7/687 (2067 words)

	Vector Labs
		Vector Laboratories has been at the forefront of developing novel, innovative labeling and detection reagents for over a quarter of a century.
		Immunohistochemistry has certainly evolved over the last 25 years to become an invaluable technique in many facets of biological science.
		A free poster is available featuring examples of single and multiple antigen labeling on tissue sections using immunohistochemistry.
	www.vectorlabs.com /products.asp?catID=35&locID=0 (337 words)

	Anatomic Pathology - Immunohistochemistry
		The immunohistochemistry lab at OHSU offers a menu of over 150 immunohistochemical tests for formalin-fixed, paraffin-embedded tissue (see searchable list).
		The majority of these studies are performed on Ventana automated immunostainers.
		The lab is also responsible for immunofluorescent staining of frozen tissue on medical and transplant renal cases.
	www.ohsu.edu /pathology/win/AnPathimmuno.htm (78 words)

	Nan Ping Wang MD, PhD is Director of Immunohistochemistry at Washington Pathology Consultants WPC at Swedish in Seattle (Site not responding. Last check: 2007-11-05)
		Nan Ping Wang MD, PhD is Director of Immunohistochemistry at Washington Pathology Consultants WPC at Swedish in Seattle
		Dr. Wang has special interests in immunohistochemistry and molecular biology; both are considered essential in the contemporary practice of pathology, particularly for the diagnosis and prognosis of tumors.
		With fellowship training in both areas, Dr. Wang serves as the Director of the Immunohistochemistry Laboratory, and oversees the daily laboratory operation to provide services to both Swedish Hospital and other clients in the region.
	washingtonpathology.com /pages/wang.htm (89 words)

	Reproductive Immunology, Immunohistochemistry: Ob/Gyn (Site not responding. Last check: 2007-11-05)
		Immunohistochemistry is a method used to localize a particular protein in a tissue.
		Label glass immunohistochemistry trays: Xylene (4), 100% EtOH (3), 70% EtOH, 50% EtOH, MetOH/ H202, DD H20, PBS, 1%Triton, 0.5% Triton, and Hematoxylin.
		Make sure there is enough liquid in each glass tray to cover the slides entirely (about 200 ml).
	info.med.yale.edu /obgyn/reproimmuno/projects/immunohistochemistry.html (948 words)

	Books for Pathologists - Immunohistochemistry
		Hayat: Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas (Vol 2)
		Hayat: Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas (Vol 3) : Molecular Genetics: Liver and Pancreatic Carcinomas
		This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method.
	www.pathologyoutlines.com /booksIHC.html (482 words)

	Immunohistochemistry (Site not responding. Last check: 2007-11-05)
		The NCI at Frederick (SAIC Frederick) Antibody Database for Mouse Antigens
		Immunohistochemistry at the NIEHS Laboratory of Experimental Pathology
		Special Techniques for Immunohistochemistry and In Situ Hybridization
	www.niaid.nih.gov /dir/services/animalcare/VetPathology/Immunohistochemistry.html (333 words)

	Histology Immunohistochemistry Service
		continues to provide routine histology and expert immunohistochemistry services to UMCCC investigators using a recharge system.
		The HIS currently employs 3 research associates, up from 2 the previous year.
		The HIS lab has established protocols for 241 antibodies with 91 routinely offered.
	www.pathology.med.umich.edu /giordano_lab/histology.htm (147 words)

	The Society for Applied Immunohistochemistry
		The Society for Applied Immunohistochemistry was originally established as the Immunohistopathology Study Group in October of 1991 by a group of pathologists and laboratory scientists working in the field of Immunohistochemistry and was incorporated in 1993.
		The Society for Applied Immunohistochemistry is pleased to announce the The First International Course on Applied Immunohistochemistry and Molecular Morphology to be held on January 23-26, 2007 on Duck Key, Florida, USA.
		The first full course devoted solely to issues in IHC and allied techniques has bee organized by Dr. Hadi Yaziji and promises to be an exciting, detailed and informative 4 days.
	www.appliedimmuno.org (284 words)

	Using LR White for Immunohistochemistry Technical Data Sheet
		STERNBERGER, L.A., HARDY, P.H. Jr., CUCULIS, J.J. and MEYER, H.G., The unlabelled antibody enzyme method of immunohistochemistry.
		Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in indentification of spirochetes.
		Using LR White for Immunohistochemistry is available online from the EMS Catalog.
	www.emsdiasum.com /microscopy/technical/datasheet/14380im.aspx (1017 words)

	Applied Immunohistochemistry & Molecular Morphology (Site not responding. Last check: 2007-11-05)
		This journal provides complete coverage of the diagnostic and prognostic applications of immunohistochemistry (IHC), its contributions to the understanding of the biology of tum">
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		This journal provides complete coverage of the diagnostic and prognostic applications of immunohistochemistry (IHC), its contributions to the understanding of the biology of tumors and other lesions, and the technical aspects...more.
	lww.com /productdetailresults/1,2265,1062-3345,00.html (185 words)

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