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Topic: Immunophenotyping

In the News (Tue 22 Dec 09)

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	Hematopathology: Immunophenotyping Lymphomas (Site not responding. Last check: 2007-10-13)
		The technique is called "immunophenotyping" for 2 reasons: 1) It is dependent on the activity of antibodies, which are immunological substances 2) It is used chiefly to identify lymphoid and hematopoietic cells, which are part of the immune system.
		Immunophenotyping is used clinically chiefly to diagnose and categorize lymphomas and leukemias.
		Immunophenotyping is helpful because in many instances different kinds of benign and malignant lymphoid cells resemble each other in routinely stained tissue sections and smears.
	pleiad.umdnj.edu /hemepath/immuno/immuno.html (1060 words)

	Consensus Leukemia Immunophenotyping
		The scope of flow cytometric immunophenotyping is to provide an objective and reproducible method for the diagnosis and therapy control of hematological malignancies, based on the immunological identification and characterization of clonally expanded abnormal precursor cells of the various hematopoietic lineages.
		Immunophenotyping also contributes to the further characterization of AML subtypes, especially the M0 and M7 subtype, while the FAB-definition of M1 to M6 is still based mainly on morphology and cytochemistry.
		The major goals of immunophenotyping in mature lymphoid neoplasias currently are, therefore, the assignment of abnormal cells to the B or T cell lineage, their maturational analysis, and the characterization of specific phenotypes which might be helpful for the subclassification of disease.
	www.biochem.mpg.de /valet/eurocel2.html (11271 words)

	Immunophenotyping of Bronchoalveolar Lavage Lymphocytes -- Harbeck 5 (3): 271 -- Clinical and Vaccine Immunology
		Immunophenotyping of Bronchoalveolar Lavage Lymphocytes -- Harbeck 5 (3): 271 -- Clinical and Vaccine Immunology
		absence of uniformity in lymphocyte immunophenotyping of BAL fluids
		Immunophenotyping of lymphocyte subsets in bronchoalveolar lavage fluid.
	cdli.asm.org /cgi/content/full/5/3/271 (4838 words)

	IMMUNOPHENOTYPING OF ACUTE LEUKEMIA AT KING FAISAL SPECIALIST HOSPITAL AND RESEARCH CENTRE:15(2)
		We conclude that immunophenotyping by flow cytometry is a useful and reliable method for classification of acute leukemia, especially when interpreted in the light of morphology and cytochemistry.
		Eleven bone marrows submitted for immunophenotyping with the clinical diagnosis of chronic lymphocytic leukemia and bone marrow involvement by lymphoma, as well as 13 bone marrows submitted showing no clonal expansion, with the diagnosis of chronic granulocytic leukemia in questionable transformation which turned out to be in chronic phase morphologically, were excluded from the study.
		Bowman GP, Neame PB, Soambonsrup P. The contribution of cytochemistry and immunophenotyping to the reproducibility of the FAB classification in acute leukemia.
	www.kfshrc.edu.sa /annals/152/94109ar.html (1599 words)

	Clinically Useful Information Provided by the Flow Cytometric Immunophenotyping of Hematological Malignancies: Current ...
		Immunophenotyping in the diagnosis of chronic lymphoproliferative disorders.
		Immunophenotyping investigation of minimal residual disease is a useful approach for predicting relapse in acute myeloid leukemia patients.
		Coordinate expression of T-cell antigens on acute myelogenous leukemia and of myeloid antigens on T-acute lymphoblastic leukemia.
	www.clinchem.org /cgi/content/full/45/10/1708 (6829 words)

	Acute Leukemia Profile, Flow Cytometry (Site not responding. Last check: 2007-10-13)
		Multiparameter analysis using four-color immunophenotyping techniques is a rapid and specific method of assigning lineage in acute leukemia.
		When the morphology and immunophenotype suggest a diagnosis of acute promyelocytic leukemia (APL), confirmation by testing for the retinoic acid receptor gene rearrangement by cytogenetic or molecular methods is recommended.
		Immunophenotyping and cytogenetic analysis are increasingly being used to supplement the traditional methods (morphology and cytochemistry) of classifying acute leukemias and to provide prognostic information.
	www.labcorp.com /datasets/labcorp/html/chapter/mono/nf10005430.htm (467 words)

	1997 Revised Guidelines for Performing CD4+ T-Cell Determinations...
		Immunophenotyping refers to the detection of antigenic determinants (which are unique to particular cell types) on the surface of WBCs using antigen-specific monoclonal antibodies that have been labeled with a fluorescent dye or fluorochrome (e.g., phycoerythrin {PE} or fluorescein isothiocyanate {FITC}).
		The fluorochrome-labeled cells are analyzed by using a flow cytometer, which categorizes individual cells according to size, granularity, fluorochrome, and intensity of fluorescence.
		To ensure accurate results for specimens from HIV-infected persons, laboratories must validate their hematology instrument's ability to give the same result at time 0 and at the maximum time claimed by the manufacturer when using specimens from both persons infected with HIV and those not infected.
	wonder.cdc.gov /wonder/PrevGuid/m0045580/m0045580.asp (8626 words)

	Performance of CD4+ T-Cell Determinations in Persons with HIV Inf
		Immunophenotyping relies on detecting specific antigenic determinants on the surface of WBCs by antigen-specific monoclonal antibodies that have been labeled with a fluorescent dye or fluorochrome, such as phycoerythrin (PE) or fluorescein isothiocyanate (FITC).
		For this reason, the immunophenotyping results should be reviewed carefully to ensure that T-cell levels are similar to those determined previously with the full recommended panel.
		Three test components contribute to intra- and interlaboratory variability when lymphocyte populations are measured by immunophenotyping: a) whole blood immunophenotyping preparation and analysis; b) total WBC count; and c) leukocyte differential.
	wonder.cdc.gov /wonder/prevguid/m0019952/m0019952.asp (5475 words)

	Diagnostic utility of flow cytometric immunophenotyping in myelodysplastic syndrome -- Elghetany ; et al. 99 (1): 391 ...
		Diagnostic utility of flow cytometric immunophenotyping in myelodysplastic syndrome.
		Diagnostic flow cytometric immunophenotyping in myelodysplastic syndrome: the US-Canadian consensus recommendations on the immunophenotypic analysis of hematological neoplasia by flow cytometry apply
		U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures.
	www.bloodjournal.org /cgi/content/full/99/1/391 (1369 words)

	Correction for erythroid cell contamination in microassay for immunophenotyping of neonatal lymphocytes -- de Vries et ...
		Immunophenotyping of blood lymphocyte subpopulations in neonates and young infants is hampered by the limited amount of blood
		Immunophenotyping of blood lymphocyte subpopulations is an important tool in the diagnosis and follow up of children with
		Immunophenotyping of blood lymphocytes in childhood: Reference values for lymphocyte subpopulations.
	fn.bmjjournals.com /cgi/content/full/80/3/F226 (1579 words)

	Diagnostic utility of flow cytometric immunophenotyping in MDS (Site not responding. Last check: 2007-10-13)
		Flow cytometric immunophenotyping is an accurate and highly sensitive method for detection of quantitative and qualitative abnormalities in hematopoietic cells.
		The immunophenotypic abnormalities associated with MDS were defined, and the diagnostic utility of flow cytometry was compared, with morphologic and cytogenetic evaluations in 20 difficult cases.
		Although morphology and cytogenetics were adequate for diagnosis in most cases, flow cytometry could detect immunophenotypic abnormalities in cases when combined morphology and cytogenetics were nondiagnostic.
	home.earthlink.net /~cfelten/diagnostic_utility_of_flow_cytom.htm (230 words)

	Immunophenotyping
		Immunophenotyping is the analysis of heterogeneous populations of cells for the purpose of characterizing the presence and proportions of the various populations of interest.
		LSC-based immunophenotyping is a well-developed application in clinical research settings, well-suited to paucicellular specimens such as FNAs and pediatric blood.
		Immunophenotyping on the iGeneration allows samples to be analyzed with higher throughput in a multi-well plate.
	www.compucyte.com /immunophenotyping.htm (415 words)

	Table of contents for Library of Congress control number 2001004704 (Site not responding. Last check: 2007-10-13)
		Immunophenotypic markers in the diagnosis of non-Hodgkin's lymphomas Andr s Matolcsy Lineage of non-Hodgkin's lymphomas B cell non-Hodgkin's lymphomas T/NK cell non-Hodgkin's lymphomas 4.
		Clinical application of immunophenotyping in immunodeficiencies Katalin P l¢czi Congenital (primary) immunodeficiencies Cellular and combined immunodeficiency disease Predominantly antibody deficiency diseases Phagocytic cell disorders Human immunodeficiency virus infection and acquired immunodeficiency syndro 5.
		Immunophenotyping in hematopoietic stem cell transplantation Katalin P l¢czi Use of CD34 for identification of hematopoietic progenitors Reconstitution of hematopoiesis posttransplant and normal hematopoiesis dur ng ontogeny Effect of GVHD on immunological reconstitution Multi-tissue potential of hematopoietic stem cell Index
	www.loc.gov /catdir/toc/ecip02/2001004704.html (158 words)

	News - Fine-Needle Cytology With Immunophenotyping Accurate in Diagnosing Lymphomas (Site not responding. Last check: 2007-10-13)
		By Kurt Ullman, RN Fine-needle aspiration cytology (FNAC) appears to be an accurate method of diagnosing lymphomas when corroborated by immunophenotyping, according to a recent study.
		A total of 103 patients with lymphadenopathy and accessible lymph nodes seen by Ola Landgren, MD, and colleagues at the Karolinska Hospital and Institutet in Stockholm, Sweden, underwent both diagnostic procedures.
		Immunophenotyping was performed on both FNAC and histopathological specimens.
	docguide.com /news/content.nsf/news/8525697700573E1885256E2A0038EE88 (511 words)

	About Flow Cytometry (Site not responding. Last check: 2007-10-13)
		Basically, a "generic" flow cytometer is similar to a hematology cell counter, using a 488 nm argon ion laser as an excitation light source and being equipped with additional detectors for fluorescence signal measurement.
		Dot plots are appropriate when the number of cells available for analysis is not limiting, and, where the fluorescence intensity associated with cells that are positive for the markers in question is bright enough so that there is no overlap with the cells that are negative for each marker.
		Data reporting in immunophenotyping of hemo-lymphatic neoplasia is shifting away from reporting percentages of positive cells toward one where patterns of reactivity are examined, with results being reported in qualitative (ie, positive, negative) and staining intensity (ie, weak, strong) terms.
	flowsite.hitchcock.org /AboutFlow/main.html (3959 words)

	Immunophenotyping Congenital Leukemia (Site not responding. Last check: 2007-10-13)
		Historically, a large proportion of congenital leukemias have been thought to be of the myeloid lineage, in contrast to pediatric leukemias in general which are primarily lymphoid.
		The precise proportions of the lineage assignments may be distorted by the inclusion of cases of transient myeloproliferative disorders (TMD) as congenital leukemia.
		The immunophenotyping data available to date suggest that congenital leukemias are phenotypically heterogeneous lacking any common distinguishing markers.
	www4.umdnj.edu /cytoweb/congen.htm (151 words)

	Immunophenotyping of Acute Leukemias Using a Quartz Crystal Microbalance and Monoclonal Antibody-Coated Magnetic ...
		Immunophenotyping, which utilizes panels of lineage-associated monoclonal antibodies to recognize clusters of differentiation (CD) antigens expressed on various leukocytes, plays a key role in the clinical diagnosis of acute leukemias.
		The response characteristics of the developed QCM system for immunophenotyping various lineage-defined leukocytes were investigated in detail.
		Moreover, the applicable feasibility of the QCM immunophenotyping method was validated through assessing a number of clinical specimens, which phenotypes are in acceptable agreement with those obtained by the immunoenzyme assay clinically used for this purpose.
	pubs.acs.org /cgi-bin/abstract.cgi/ancham/2006/78/i08/abs/ac051286z.html (280 words)

	Immunophenotyping - Wikipedia, the free encyclopedia
		Immunophenotyping is a technique notably used in the diagnosis of leukemia.
		It involves the labelling of white blood cells with antibodies directed against surface proteins on their membrane.
		Immunophenotyping Lymphomas, University of Medicine and Dentistry of New Jersey
	en.wikipedia.org /wiki/Immunophenotyping (137 words)

	A MULTIPARAMETRIC WAY TO IMMUNOPHENOTYPING
		A classic practical example of this procedure is seen in the opportunity it affords of simultaneously obtaining data for distribution of two or more membrane antigens in cell populations of varying sizes, distinguishable therefore on the basis of their physical characteristics.
		There is no doubt that a multiparameter approach to immunophenotyping has been made possible in recent years by a series of steps ahead in instrumentation and reagent technology.
		It is still particularly interesting to note that what made this approach necessary were, so to speak, a series of "cultural" factors that have modified the application of flow cytometry to laboratory diagnostics.
	www.cyto.purdue.edu /flowcyt/research/cytotech/amfc/data/page9.htm (3361 words)

	Table of Contents for Immunophenotyping
		Clinical Applications of Flow Cytometric Immunophenotyping in Acute Lymphoblastic Leukemia (J. DiGiuseppe & M. Borowitz).
		Use of Multiparameter Flow Cytometry and Immunophenotyping for the Diagnosis and Classification of Acute Myeloid Leukemia (G. Stelzer & L Goodpasture).
		Immunophenotyping in HIV Infection (J. Nicholson & F. Mandy).
	www.omega.com /bobi/Table_of_Contents.asp?id=CH-5615 (121 words)

	Immunophenotyping of Leukemias Using a Cluster of Differentiation Antibody Microarray -- Belov et al. 61 (11): 4483 -- ...
		Immunophenotyping of Leukemias Using a Cluster of Differentiation Antibody Microarray -- Belov et al.
		Immunophenotyping of Leukemias Using a Cluster of Differentiation Antibody Microarray
		Immunophenotypes of normal peripheral blood leukocytes and CLL cells.
	cancerres.aacrjournals.org /cgi/content/full/61/11/4483 (4501 words)

	Negative Control Checks Fluorescence Limit
		Daily use of this standard in immunophenotyping assays can show that the flow cytometer is capable of detecting particles with lower fluorescence than unstained target cells.
		Discrimination of dimly fluorescent target cells from the negative control cells proves that the instrument is sensitive enough to perform the immunophenotyping assay.
		The Negative Control is prepared from chicken erythrocytes that have been processed to remove leukocytes.
	www.biosure.com /rpages/osmiumB.html (264 words)

	Events Details - Immunophenotyping Meeting
		This well established annual flow cytometry meeting on immunophenotyping is ideal for people in the field who like to remain updated on the recent advances in immunophenotyping and flow cytometry in general.
		The aim of the meeting is to discuss the main aspects of the use of flow cytometry in the diagnosis of leukaemia and lymphoma.
		Topics of current interest such as Zap 70 as a prognostic test in CLL, strategies for immunophenotyping of unusual specimens to diagnose haematological malignancies, paediatric acute leukaemias and multiparametric / multicolour immunophenotyping by flow, will be covered.
	www.rms.org.uk /cgi-bin/events_details.cgi?id=imm19973475 (289 words)

	IngentaConnect Flow cytometric immunophenotyping of mature lymphatic neoplasias ... (Site not responding. Last check: 2007-10-13)
		Discrimination of normal and malignant cellular immunophenotypes is the most critical step in data analysis, especially if multi-color analysis is performed on highly heterogenous cell suspensions.
		The identification of physiological and malignant cells was achieved by predefining population boundaries, based on the expectations of the population’s location in two-dimensional dot plots.
		The prospective application of these predefined region boundaries in 52 blood and bone marrow samples enabled identification of lymphoma cells with regard to their presence and immunophenotype, based on the correlation of markers as defined in multiple tubes.
	www.ingentaconnect.com /content/ios/acp/1999/00000019/00000002/acp097 (266 words)

	Evaluation of stabilized whole blood control materials for lymphocyte immunophenotyping.
		Evaluation of stabilized whole blood control materials for lymphocyte immunophenotyping.
		We evaluated stabilized whole blood control materials for their performance as a routine control material for immunophenotyping using flow cytometry.
		These materials serve as the method control, controlling for both the binding of monoclonal antibodies to the cells and the efficiency of the red-blood-cell lysing reagent.
	www.aegis.com /aidsline/2000/apr/A0040369.html (367 words)

	Immunophenotyping Reference Ranges (Site not responding. Last check: 2007-10-13)
		Immunophenotyping, as many other clinical assays, is interpreted only in the context of reference values obtained from healthy control individuals.
		While the use of these reference values, or ranges, has been commonplace in the clinical flow cytometry laboratory for well over a decade, there has been little consensus in standardizing how these values should be obtained, analyzed, or expressed.
		Additionally, examples are given of previously published reference ranges for a variety of specimens often submitted for immunophenotyping.
	www4.umdnj.edu /cytoweb/ranges.htm (122 words)

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