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Topic: In vivo staining


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In the News (Sat 20 Jul 19)

  
  Phylonix - Zebrafish Assays for Drug Screening - Online Literature
Using endogenous alkaline phosphatase staining and a whole-animal enzyme assay, we demonstrated that SU5416 and flavopiridol, compounds shown to have anti-angiogenic effects in mammals, inhibit blood vessel growth in zebrafish, and the bioassay is suitable for high throughput screening.
We also demonstrated that in vivo acridine orange staining can be used to visualize apoptotic events in embryos treated with brefeldin A, neomycin, or caspase inhibitors.
After in vivo staining, acridine orange can be extracted and quantitated using a fluorescence microplate reader, providing a screening system for agents that modulate apoptosis.
www.phylonix.com /OnlineLiterature.html   (471 words)

  
  Staining (biology) - Wikipedia, the free encyclopedia
Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes.
Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.
Gram staining uses crystal violet to stain cell walls, iodine as a mordant, and a fuchsin or safranin counterstain to mark all bacteria.
en.wikipedia.org /wiki/Staining_(biology)   (2098 words)

  
 [No title]
Stains and [[dyedyes]] are frequently used in [[biology]] and [[medicine]] to highlight structures in [[tissuebiological tissues]] for viewing, often with the aid of different [[microscopemicroscopes]].
Stains may be used to define and examine bulk tissues (highlighting, for example, [[muscle]] fibers or [[connective tissue]]), cell populations (classifying different [[blood cellblood cells]], for instance), or organelles within individual cells.
Biological staining is also used to mark cells in [[flow cytometry]], and to flag proteins or [[nucleic acidnucleic acids]] in [[gel electrophoresis]].
www.ihcworld.com /histowiki/doku.php?id=staining&do=export_raw   (2034 words)

  
 staining [HistoWiki]
This staining protocol is used frequently in histology to examine thin sections of tissue.
Although it will not stain healthy cells, it can be used to identify cells in the late stages of apoptosis; such cells have much more permeable membranes.
Acid fuchsin is anionic and is frequently used to stain cytoplasmic structures and fibres made predominantly from basic proteins, such as collagen, smooth muscle, or mitochondria.
www.ihcworld.com /histowiki/doku.php?id=staining   (1972 words)

  
 Murine in vivo CFSE Flow Kit Protocol   (Site not responding. Last check: 2007-10-15)
The in vivo Murine CFSE Flow Kit offers the unique opportunity to study the activation of alloreactive T cells and the in vivo effect of immunomodulatory therapies on alloreactive T and B cells.
Excess dye is washed away and stained cells are injected into a host mouse where they are induced to proliferate.
Staining with fluorescence labeled antibodies for cell surface molecules allows the examination of the proliferation of specific lymphocyte subsets.
www.renovarinc.com /PROTOCOLS/murine_invivo.htm   (560 words)

  
 Mesenchymal Stem Cells Acquire Characteristics of Cells in the Periodontal Ligament in vitro -- Kramer et al. 83 (1): ...
Osteocalcin staining of mesenchymal stem cells is shown in panel A (green) and panel B (brown).
Bone sialoprotein staining of mesenchymal stem cells is shown in panel A (green) and panel B (brown).
Osteopontin staining of mesenchymal stem cells is shown in panel A (green) and panel B (brown).
jdr.iadrjournals.org /cgi/content/full/83/1/27   (4179 words)

  
 In vivo two-photon calcium imaging of neuronal networks -- Stosiek et al. 100 (12): 7319 -- Proceedings of the National ...
The cells were stained in vivo, and the brain was then removed and sliced.
Squares (n = 10) represent cortices stained and imaged in vivo, and circles represent cortices imaged in vitro and stained either in vivo (n = 3, red symbols) or in vitro (n = 10, fl symbols).
The plot summarizes data obtained in seven in vivo (red squares) and nine in vitro (fl circles) experiments (left part of the graph) and in three in vitro (fl circles) experiments (right part of the graph).
www.pnas.org /cgi/content/full/100/12/7319   (4716 words)

  
 RedOrbit NEWS | Mechanism of bone formation with gene transfer of the cDNA encoding for the intracellular protein LMP-1
On the basis of its association with bone development and the results of suppression and overexpression experiments, LMP is considered to induce secretion of soluble factors that convey its osteoinductive activity and to be a critical regulator of osteoblast differentiation and maturation in vitro and in vivo3,14-16.
In vivo studies showed that most of the implanted buffy-coat cells expressing LMP-1 survived for less than a week in vivo, but there was indirect evidence of an influx of host cells that differentiated into bone- forming cells.
Finally, the in vivo experiments in human buffy-coat cells confirmed these observations in cells and an environment in which bone was actually forming to ensure that the observations were true in a physiologic bone-- formation setting.
www.redorbit.com /modules/news/tools.php?tool=print&id=3262   (3943 words)

  
 Murine in vivo CFSE Flow Kit Protocol   (Site not responding. Last check: 2007-10-15)
Staining of experimental cells: add 3 ┬Ál of control anti-IgG, anti-CD3, anti-CD4, Anti-CD8, or anti-CD19 antibody to each tube.
Detect PI staining using a 670nm long pass filter.
Analyze PE-antibody stained cells to examine phenotype (see Figures D-I).
www.renovarinc.com /PROTOCOLS/murine_invivo2.htm   (477 words)

  
 Fluorescence Properties and Staining Behavior of Monodansylpentane, a Structural Homologue of the Lysosomotropic Agent ...
In vivo staining of PaTu 8902 cells with MDC (a), MDH (b), MDOH (c), or MDA (d).
Staining of PaTu 8902 cells with LysoTracker and MDC (a,b) or with LysoTracker and MDH (c,d).
Biederbick A, Kern HF, Elsässer HP (1995) Monodansylcadaverine (MDC) is a specific in vivo marker for autophagic vacuoles.
www.jhc.org /cgi/content/full/49/2/177   (3397 words)

  
 VIVO - ABOUT US
VIVO is a unique retailer specializing in furnishings and accessories for the home, garden and office.
From the exclusive to the unusual, VIVO is the place to find an eclectic mix of fashion, elegance, and style.
Whether you are looking for new furniture or need to enhance your room with color, we offer trained designers to give you the ideas you have been waiting for.
www.vivohome.com /about.htm   (344 words)

  
 DNA - Facts, Information, and Encyclopedia Reference article
Unknotting is required for the replication of circular DNA as well as for various types of recombination in linear DNA.
A DNA segment with excess or insufficient helical twisting is referred to, respectively, as positively or negatively "supercoiled".
DNA in vivo is typically negatively supercoiled, which facilitates the unwinding of the double-helix required for RNA transcription.
www.startsurfing.com /encyclopedia/d/n/a/DNA_396c.html   (5249 words)

  
 In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice -- Sharma and Shimeld 81 (9): 785 -- ...
In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice -- Sharma and Shimeld 81 (9): 785 -- British Journal of Ophthalmology
Direct immunofluorescence was used in vivo in a masked study to diagnose HSK in mice using a standard slit lamp with cobalt
Immunofluorescence staining techniques are used routinely in vitro to diagnose infections especially viruses including HSV.
bjo.bmjjournals.com /cgi/content/full/81/9/785   (2174 words)

  
 Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures -- Meller et al. ...
Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures -- Meller et al.
Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures
Phorbol ester preferentially stimulates mouse fornical conjunctival and limbal epithelial cells to proliferate in vivo.
bjo.bmjjournals.com /cgi/content/full/86/4/463   (6119 words)

  
 DFU: Institut for Farmakologi: In vitro in vivo farmakologi   (Site not responding. Last check: 2007-10-15)
Forside > Institut for Farmakologi og Farmakoterapi > Forskning > In vitro in vivo farmakologi
In vivo imaging with luciferase luminiscence at cancer and chronic pain
The results of the in vivo animal studies point to application in the human situation.
www.dfuni.dk /index.php/In_vitro_in_vivo_farmakologi/2765/0   (404 words)

  
 Faculty Publications 94   (Site not responding. Last check: 2007-10-15)
Doane, M.G.: Abnormalities of the structure of the superficial lipid layer on the in vivo dry-eye tear film.
Ehinger, B., Zucker, C.L., Bruun, A., and Adolph, A.: In vivo staining of oligodendroglia in the rabbit retina.
Perng, G.-C., Dunkel, E.C., Geary, P.A., Slanina, S.M., Ghiasi, H., Kaiwar, R., Nesburn, A.B., and Wechsler, S.L.: The latency-associated transcript gene of herpes simplex virus type 1 (HSV-1) is required for efficient in vivo spontaneous reactivation of HSV-1 from latency.
www.schepens.harvard.edu /faculty_publications_94.htm   (4356 words)

  
 Migration of Lymphoid Cells from Vaginal Epithelium to Iliac Lymph Nodes in Relation to Vaginal Infection by Herpes ...
Staining of the stroma is limited to a few regions adjacent to the epithelium and is less bright (large arrow).
Migration of stained cells from superficial vaginal mucosa to iliac lymph nodes and axillary lymph nodes
The stained nucleus is similar in size and shape to the nuclei of small lymphocytes.
www.jimmunol.org /cgi/content/full/160/3/1173   (6384 words)

  
 3DM PuraMatrix Publications
PDGF-BB with nanofibers induced PDGFR-beta and Akt phosphorylation in cardiomyocytes in vivo.
Furthermore, the self-assembling peptide nanofiber microenvironments recruit progenitor cells that express endothelial markers, as determined by staining with isolectin and for the endothelial-specific protein platelet— endothelial cell adhesion molecule-1.
Isolation and expansion of self-renewing neural cells ex vivo are required for neural tissue repair in regenerative medicine.
www.puramatrix.com /pubs/index.htm   (6925 words)

  
 Biophysics - Wikipedia, the free encyclopedia
Traditional studies in biology are conducted using statistical ensemble experiments, typically using pico- to micro-molar concentrations of macromolecules.
Because the molecules that comprise living cells are so small, techniques such as PCR amplification, gel blotting, fluorescence labeling and in vivo staining are used so that experimental results are observable with an unaided eye or, at most, optical magnification.
Using these techniques, biologists attempt to elucidate the complex systems of interactions that give rise to the processes that make life possible.
en.wikipedia.org /wiki/Biophysics   (898 words)

  
 InvivoGen: Delivering Genes
Two types of test are usually used to detect the presence of mycoplasma: PCR and DNA fluorescent staining.
The DNA fluorescent staining method uses fluorochromes such as DAPI and Hoechst 33258 that bind specifically to DNA; mycoplasma infected cells are seen as fluorescing nuclei with extranuclear mycoplasmal DNA whereas uninfected cells show only fluorescing nuclei.
The recommended suppliers of DNA fluorescent staining kits are Sigma and ICN.
www.invivogen.com /faq.php?ID=3   (1240 words)

  
 HSulf-1 and HSulf-2 Are Potent Inhibitors of Myeloma Tumor Growth in Vivo -- Dai et al. 280 (48): 40066 -- Journal of ...
Bottom row, primary tumors were stained with Masson's trichrome stain, which colors collagen blue and nuclei and cytoplasm red.
in vivo is restricted to the tumor cell surface.
and staining is predominantly in the tumor stroma.
www.jbc.org /cgi/content/full/280/48/40066   (5816 words)

  
 Cancer Immunity 2:14 (2002)
Enriched telomerase-specific CD8 T cells have so far not been observed ex vivo, even though telomerase is expressed by many tumors (16).
Lack of tumor cell recognition may be due to inefficient antigen processing and presentation, or because of low TCR avidity.
In vitro specificity and efficacy of T cell recognition needs to be analyzed carefully to evaluate the potential for in vivo protective immunity and immunotherapy.
www.cancerimmunity.org /v2p14/021014.htm   (2062 words)

  
 HindIII Liposomes Suppress Delayed-Type Hypersensitivity Responses In Vivo and Induce Epidermal IL-10 In Vitro -- ...
HindIII Liposomes Suppress Delayed-Type Hypersensitivity Responses In Vivo and Induce Epidermal IL-10 In Vitro -- Nishigori et al.
HindIII Liposomes Suppress Delayed-Type Hypersensitivity Responses In Vivo and Induce Epidermal IL-10 In Vitro
staining as an additional marker of the in vivo activity of
www.jimmunol.org /cgi/content/full/161/6/2684   (4319 words)

  
 [No title]
Bukilica1, S. Andrejevic2, B. Bonaci-Nikolic2, M. Nikolic3 1Institute of Rheumatology, 2Institute of Allergology and Immunology, 3Institute of Dermatology, Belgrade, Yugoslavia Epidermal in vivo nuclear staining is occasionally noted in lupus band test (LBT), in patients with connective tissue diseases (CTD).
The exact clinical significance of this finding remains unelucidated, especially in the association with the positive LBT.
Between 1990-1999, speckled IgG staining in keratinocyte nuclei was observed in 31 LBT specimens.
www.kenes.com /autoim2002/Scientific/268.doc   (127 words)

  
 In vivo detection of amyloid plaques in a mouse model of Alzheimer's disease -- Skovronsky et al. 97 (13): 7609 -- ...
In vivo detection of amyloid plaques in a mouse model of Alzheimer's disease -- Skovronsky et al.
In vivo detection of amyloid plaques in a mouse model of Alzheimer's disease
After imaging for BSB staining, sections were stained with TF-S and reimaged (B and D).
www.pnas.org /cgi/content/full/97/13/7609   (4178 words)

  
 Upregulation of CD40 Expression on Endothelial Cells Infected with Human Cytomegalovirus -- Maisch et al. 76 (24): ...
Immunohistochemical staining of tissue sections from a patient with acute HCMV gastroenteritis was performed.
Viral IE antigen and CD40 were simultaneously detected by indirect immunoperoxidase staining and indirect alkaline phosphatase staining, respectively, as described in Materials and Methods.
Nuclear staining of HCMV-infected cells is brown, and cytoplasmic staining of CD40 antigen is red.
jvi.asm.org /cgi/content/full/76/24/12803   (5868 words)

  
 Visualization of Cell Death In Vivo during Murine Endotoxin-Induced Uveitis -- Yang et al. 44 (5): 1993 -- ...
TUNEL Staining on the Iris and Corneal Wholemounts
Cells labeled with annexin V and/or propidium iodide in wholemounts and apoptotic cells identified with TUNEL staining in the ocular tissues or wholemounts after endotoxin injection into the vitreous body.
Cell membranes were stained with annexin V and nuclei were stained with propidium iodide.
www.iovs.org /cgi/content/full/44/5/1993   (3635 words)

  
 WHFoods: Turmeric, ground
To avoid a lasting stain, quickly wash any area with which it has made contact with soap and water.
Curcumin is an in vivo inhibitor of angiogenesis.
Curcumin inhibits proliferation, induces apoptosis, and inhibits angiogenesis of LNCaP prostate cancer cells in vivo.
www.whfoods.com /genpage.php?tname=foodspice&dbid=78   (4613 words)

  
 ANDROGEN-INDEPENDENT KGF EXPRESSION   (Site not responding. Last check: 2007-10-15)
Immunohistochemical staining revealed KGF to be present in the stroma throughout the prostate, regardless of the functional region, and staining for KGF remained high through 21 days post- castration.
Furthermore, no significant change in either stromal staining or message for KGF was observed in newborn rat prostates ten days after castration, suggesting a similar regulatory mechanism for KGF in the adult and immature prostate.
These observations suggest the presence of KGF protein is not related to functional differences in the prostate epithelium, and that expression of KGF in vivo is not greatly influenced by androgen.
journals.endocrinology.org /joe/156/joe1560115.htm   (323 words)

  
 Delayed Wound Healing in Keratin 6a Knockout Mice -- Wojcik et al. 20 (14): 5248 -- Molecular and Cellular Biology
All samples were stained with antibodies to MK14 (red) and MK6 (yellow).
Note that in order to show a well-preserved wound, fixed tissue, on which this MK6 antibody results in weaker staining of the basal layers, is shown; however, this is not the case on unfixed frozen sections (not shown).
Keratins (K16 and K17) as markers of keratinocyte hyperproliferation in psoriasis in vivo and in vitro.
mcb.asm.org /cgi/content/full/20/14/5248   (6355 words)

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