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Topic: Incubate (biology)

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	Avian incubation - Wikipedia, the free encyclopedia
		The word incubate in the context of birds refers to the development of the chick (embryo) within the egg and the constant temperature required for the development of it over a specific period.
		Possibly the most common pattern is that the female does all the incubation, as in the Coscoroba Swan and the Indian Robin, or most of it, as is typical of falcons.
		In these latter, the incubation is interrupted; the longest uninterrupted period is 64 to 67 days in the Emperor Penguin.
	en.wikipedia.org /wiki/Incubate (397 words)

	Bird - Wikipedia, the free encyclopedia
		In other words, in bird species in which male incubation is common, females tend to select mates on the basis of parental behaviors rather than physical appearance.
		Chickens and pigeons are popular as experimental subjects, and are often used in biology and comparative psychology research.
		As birds are very sensitive to toxins, the Canary was used in coal mines to indicate the presence of poisonous gases, allowing miners sufficient time to escape without injury.
	en.wikipedia.org /wiki/Bird (2106 words)

	Hotbed - Wikipedia, the free encyclopedia
		Colloquially, it is often heard in the phrase, "a hotbed of activity." The term has varying, specific meanings in diverse fields, such as economics and biology.
		In biology, a hotbed is a pile of decaying organic matter warmer than its surrounding due to the heat given off by the metabolism of the microorganisms in the decomposing pile.
		Hotbeds are used by humans for composting and keeping delicate plants warm during the winter.
	en.wikipedia.org /wiki/Hotbed (124 words)

	Aristotle's Biology (Site not responding. Last check: 2007-10-23)
		Aristotle was able to accomplish what he did in biology because he had given a great deal of thought to the nature of scientific inquiry.
		I began by noting that it is a fundamental aspect of Aristotle's theory of science that investigation proceeds from a stage where one establishes the existence of the things being studied and facts about them to a stage where the focus is on causal explanations by reference to the natures and essences of things.
		“Aristotle's biology was not essentialist,” in Gotthelf and Lennox 1987, pp.
	plato.stanford.edu /entries/aristotle-biology (11022 words)

	Biochemistry and molecular biology
		Incubate the screen plates for 4–5 days at 30°C. Pick any growing colonies (that are clearly bigger than background colonies, this varies from bait to bait) and patch with a sterile toothpick on a fresh Sc-Leu,-Trp,-His + 3AT plate.
		Incubate at least 30 minutes at 30°C. Heat-shock the reactions for exactly 20 minutes at 42°C. Quickly centrifuge the tubes in a microcentrifuge, 5 seconds is sufficient.
		Incubate 1 ml sepharose protein-A beads (Pharmacia) at 20°C with mouse ascite (2 mg of MAb12CA5(HA) and MAb9E10 (Myc) antibodies).
	www.wormbook.org /chapters/www_biochemmolbio/biochemmolbio.html (3555 words)

	Untitled Document (Site not responding. Last check: 2007-10-23)
		Incubate in shaker at 37 C for 1 hr.
		Incubate colonies overnight at 37°C and pick when 1-2mm in diameter.
		Incubate colonies at 37°C and pick when 1-2mm in diameter.
	www.ag.iastate.edu /departments/eeob/faculty/WendelJ/cloning.htm (440 words)

	Cell Biology II Exam #2
		Incubate with anti rab3A Ab tagged with large gold particles and with an Ab to acid phosphatase (lysosomal enzyme) tagged with small gold particles.
		Incubate M9 sequences and complete Al proteins either with reticulocyte lysate (cytosol source) or with buffer or with purified transportin.
		Incubate "S Methionine and "S cysteine labeled transportin with streptavidin-agarose beads bound to biotinylated bovine serum albumin-NES conjugate (30 min at room temperature in PBS) while increasing the concentration of the inhibitor.
	users.von.uc.edu /Ipws/Cell2Exams/Cell_II_exam_2.html (4929 words)

	General Guidelines for Immunostaining
		Incubate the coverslips with 100 µl monoclonal antibody diluted in PBS for 60 min.
		Incubate the coverslips with 100,ul monoclonal antibody diluted in PBS for 60 min.
		Incubate with FITC or TRITC labeled second antibody directed against mouse IgG at appropriated dilution.
	www.biodesign.com /tables/bio12p.html (1154 words)

	Vector Labs
		Incubate sections for 30 min with biotinylated secondary antibody from the 1st VECTASTAIN
		For a 10 min incubation, double the concentration of the biotinylated antibody and normal serum.
		Incubate sections for 30 min with biotinylated secondary antibody appropriate for labeling the 2nd primary antibody diluted in 5% NS.
	www.vectorlabs.com /infopage.asp?dpID=27&locID=507091 (805 words)

	Research Link 2000: Transformation of Yeast
		One of the most important techniques of modern molecular biology is the ability to introduce a specific gene into an organism and have that genetic information expressed by the organism.
		Incubate the plate at room temperature or in a 30 C. incubator for 1-2 days.
		Incubate all plates at 30 C. or room temperature until single colonies form and their color can be determined.
	www-personal.ksu.edu /~bethmont/rl2k/transform.html (1318 words)

	Molecular Biology – DNA & RNA
		The supernatant was incubated with cAMP agarose (Sigma) in the presence
		Vortex (10sec) and incubate on ice for 10min.
		Incubate at RT for 20-25min or at 30
	www.uvm.edu /~akhowe/HoweLabProtocols3.html (4768 words)

	Aviva Systems Biology - AVIVA Systems Biology (Site not responding. Last check: 2007-10-23)
		Transfer the separated proteins onto an Immobilon P membrane (Millipore) and incubate the membrane for 1 hour at room temperature or overnight at 2-8 °C in Blocking Solution (1 X PBS, pH 7.4 containing 5% dry milk).
		Incubate the membrane for 2 hours at room temperature or overnight at 2-8 °C in Blotting Buffer (1 X PBS, pH 7.4 containing 5% dry milk unless otherwise indicated) containing primary antibody (0.2 to 1ug/ml concentration as indicated on datasheet).
		Incubate the membrane at room temperature for 1 hour in Blotting Buffer containing an appropriate secondary reagent such as goat anti rabbit IgG or anti-mouse IgG -HRP at 1:10,000 (Catalog # AviHRP-GAR).
	www.avivasysbio.com /commerce/misc/immunoblot.jsp (404 words)

	Molecular Biology Related Protocols (Site not responding. Last check: 2007-10-23)
		PCR is now one of the most widely used techniques in molecular biology; it is rapid, relatively inexpensive, and a simple means of amplifying DNA from a minute amount of template DNA.
		incubate tubes in the racks at 55°c in the incubation oven.
		Incubate on ice in the dark for 30 min.
	www.uchsc.edu /misc/diabetes/derc/prot_molecular.htm (4054 words)

	Direct genomic selection - Nature Methods
		Incubate the reactions at 11 °C for 30 min.
		Denature the DNA at 95 °C for 5 min and incubate at 65 °C for 15 min.
		Incubate the hybridization reaction at 65 °C for 70 h.
	www.nature.com /cgi-taf/DynaPage.taf?file=/nmeth/journal/v2/n1/full/nmeth0105-63.html (2909 words)

	[No title] (Site not responding. Last check: 2007-10-23)
		Molecular biology experiments often require preparation of small amounts of DNA from many samples.
		Incubate the tubes at 65 degrees C for 5-7 min.
		Spin for 3 min, discard the supernatant and invert the tubes on a paper towel to wick away the excess liquid.
	www.fgsc.net /fgn/irelan.html (400 words)

	BioSupplyNet.com - Biomedical Research Supply Information Source
		Incubate the slices in 100 µl of 100 mM NH for 30 min.
		If Coomassie staining was used to visualize the proteins and a strong blue color persists, incubate for a further 15 min with continuous gentle vortexing.
		Centrifuge the trypsin digests briefly in a microcentrifuge and transfer the peptides (in the supernatant) to a 0.5-ml microfuge tube and reserve.
	www.biosupplynet.com /cfdocs/protocols/protocol3.cfm?id=7 (926 words)

	Methods FAQ list
		Incubate at 37 degrees C with shaking until the culture is at late log phase.
		Incubate with shaking for exactly 2.5 hours at 37 degrees C. Add chloramphenicol to the culture to a final concentration of 150 ug to 170 ug/ml.
		Incubate at 16 C overnight by emersing the entire tube beneath the suface of a water bath in the cold room.
	www.lecb.ncifcrf.gov /~pnh/FAQlist.html (12573 words)

	Incubate suspension cells? - BioForum
		Just place into a standard 5% CO2 incubator on a slant to stop media getting into the lid area of the flask.
		If they do stick, you can place a rotating shaker into the incubator (most incubators have a hole in the back for the cord) and then gently shake the cells while incubating.
		If however, you require larger volumes and have spinner flasks, then you'll need the spinner plate in the incubator and again, gently spin the cells.
	www.protocol-online.org /forums/index.php?showtopic=5803 (323 words)

	Genomic DNA Extraction Methods
		Incubate samples, shaking, in tightly capped tubes, 12 to 18 hours at 50C
		Incubate 2 to 3 minutes at 45C to elute DNA from the glass beads.
		Incubate 10 to 60 minutes at 65C with occasional mixing.
	www.chemicon.com /techsupp/genomic.asp (707 words)

	Biochemicals for research and pharmaceutical production - Associates of Cape Cod, Inc. and Seikagaku Corporation
		Incubate at 30oC without shaking for 30 minutes.
		Filtered sterilized 1 M DTT stock solution (molecular biology grade) is prepared and frozen in 500 m1 aliquots.
		To protoplast the remaining yeast, at 13 ml either Lyticase or Zymolyase to their respective tubes and incubate for the determined time to reach 70% protoplast efficiency.
	www.acciusa.com /bio/bio_compstudies.html?id=29 (1686 words)

	ELISA
		Incubate at 37 °C for 30 min., or incubate (covered) overnight at 4 °C. Remove the coating solution.
		During the last incubation and immediately before use, prepare the enzyme substrate or bring the premade liquid substrate to room temperature.
		Incubate the plate (covered) for 1 hour at 37 °C. Remove the coating solution.
	www.sigmaaldrich.com /Area_of_Interest/Life_Science/Antibody_Explorer/Procedures/ELISA.html (1033 words)

	Molecular Biology Protocol Title
		Incubate at 37°C for 5 minutes to anneal oligo d(T)n to poly(A)n tail of mRNA molecules
		Incubate at 42°C for 60 minutes to reverse transcribe the mRNA molecules (meanwhile assemble PCR mix)
		Incubate at 70°C for 10 minutes to denature RTase, and quench on ice for at least 2 minutes (or store long term at -80°C) Polymerase chain reaction
	www.bioinformatics.vg /Methods/RT-PCR_protocol.shtml (493 words)

	ABI Online: Products: Cell Biology Products
		Incubate activated cells 5 days at 37°C in 5% CO Wash cells 3 times in RPMI-1640 fetal bovine serum 10%.
		Following induction with PHA, the leukocytes are incubated for 48-72 hours in RPMI-1640 and the crude TCGF fluids harvested.
		Crude TCGF fluids are clarified by centrifugation, purified by chromatographic methods and the purified TCGF sterile filtered into vials.
	www.abionline.com /products/cellbio/specs/03001.htm (307 words)

	ABLE: Molecular Genetic Analysis in Yeast
		Daniel Burke is an Associate Professor of Biology and Director of the Task Force on Science/Mathematics Education at Seton Hall University.
		After incubation, spin cells down for 30 seconds in a microfuge or for 5 minutes in a clinical centrifuge.
		Incubate both the selective plate and the non-selective master plate overnight at 30C.
	www.zoo.utoronto.ca /able/volumes/vol-11/6-burke/6-burke.htm (7269 words)

	University of North Dakota \| Biology Department
		In contrast, other hormones such as prolactin are utilized for a variety of purposes such as osmoregulation in fish, incubation behavior in birds and milk production in mammals.
		In these species, the temperature at which the eggs are incubated determines the sex of the offspring.
		Estradiol levels are altered differently under different incubation temperatures in both these reptiles.
	www.und.edu /dept/biology/fivizzani.htm (642 words)

	Methods in cell biology
		Animals are fixed by any of the methods used for antibody staining and left to incubate in a neutral buffer such as PBS or Tris-Triton.
		Incubate lysates with Protein A Sepharaose beads (100 μL per mL lysates; Amersham) or IgGsorb (100 μL per mL lysates; The Enzyme Center) for 10–30 minutes.
		Incubate approximately 5 μg of affinity purified antibodies with 3 mg total protein of embryo lysates for 2 hrs.
	dev.wormbook.org /chapters/www_intromethodscellbiology/intromethodscellbiology.html (10220 words)

	ListServ Archive: Molecular Biology Techniques
		Incubate at 56°C for 45 min allow protein degradation by proteinase K. Incubate at 95°C for 10 min to inactivate proteinase K. 2-3 µl of above sample was used for 15-20 µl PCR reaction.
		Resuspend the nuclei in 150 microliters of TE.
		Recover 450 microliters of the supernatant and precipitate with ethanol.
	dictybase.org /ListServ_archive/listserv_archive_molecbiol.html (5300 words)

	The Mr. Green Genes Wet Lab
		Compared to many other areas of biology, the technical skills required to do molecular biology are very simple; the key is to get comfortable working with small volumes.
		Bacteria are also incubated on ice to induce the low temperature adaptation of increasing membrane permeability.
		During the incubations described in steps 4 and 5, the computer-based analysis of DNA sequence data will be demonstrated for you.
	srv2.lycoming.edu /~newman/bioinformatics/mggwetlab.html (3451 words)

	Beginning Molecular Biology Laboratory Manual (Site not responding. Last check: 2007-10-23)
		Incubate both tubes at 37 deg C, shaking vigorously overnight.
		Make a note of any debris found in the tubes and only incubate longer if the culture is not dense.
		For some applications, cells can be stored at 4 deg C for short periods prior to use.
	userpages.umbc.edu /~jwolf/method2g.htm (358 words)

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