| |
| | OhioLINK ETD: Murray, Tracey |
 | | In the presence of inorganic phosphate, flavin mononucleotide (FMN) binds to the flavodoxin in two ways; phosphate-first, where the 5-phosphate group makes the initial contact with the flavodoxin, and ring-first, where contact is initiated by the isoalloxazine ring. |
 | | Thus a phosphate molecule, either the 5-phosphate group of FMN or inorganic phosphate, is required for ring-first binding to occur. |
 | | This suggested a role for Trp60 in the communication between the phosphate- and ring-binding subsites, and that Trp60 was involved in an garomatic gateh similar to one observed in the Anabaena apoflavodoxin (Genzor et al., 1996). |
| www.ohiolink.edu /etd/view.cgi?osu1060639958 (741 words) |
|