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Topic: Intein

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	Intein - Wikipedia, the free encyclopedia
		An intein is a segment of a protein that is able to excise itself and rejoin the remaining portions (the exteins) with a peptide bond.
		Inteins have been engineered for particular applications such as protein synthesis, and the selective labeling of protein segments, which is useful for NMR studies of large proteins.
		The first part of an intein name is based on the scientific name of the organism in which it is found, and the second part is based on the name of the corresponding gene or extein.
	en.wikipedia.org /wiki/Intein (632 words)

	Intein: Encyclopedia topic (Site not responding. Last check: 2007-10-21)
		Inteins have also been called "protein intron (intron: Sequence of a eukaryotic gene's DNA that is not translated into a protein) s".
		Inteins are very efficient at protein splicing and they have accordingly found an important role in biotechnology (biotechnology: The branch of molecular biology that studies the use of microorganisms to perform specific industrial processes).
		Inteins have been engineered for particular applications such as protein synthesis, and the selective labeling of protein segments, which is useful for NMR (NMR: Resonance of protons to radiation in a magnetic field) studies of large proteins.
	www.absoluteastronomy.com /reference/intein (822 words)

	BioMed Central \| Full text \| The intein of the Thermoplasma A-ATPase A subunit: Structure, evolution and expression in ...
		Inteins are selfish genetic elements that excise themselves from the host protein during post translational processing, and religate the host protein with a peptide bond.
		This intein is inserted in the same position as the inteins found in the ATPase A-subunits encoding gene in Pyrococcus abyssi, P. furiosus and P. horikoshii and is found 20 amino acids upstream of the intein in the homologous vma-1 gene in Saccharomyces cerevisiae.
		Intein excision depends on the splicing domain of the intein and the first amino acid residue of the C-extein [8].
	www.biomedcentral.com /1471-2091/2/13 (4704 words)

	Protein Splicing in the Absence of an Intein Penultimate Histidine -- Chen et al. 275 (27): 20431 -- Journal of ... (Site not responding. Last check: 2007-10-21)
		for splicing of the Mja Rpol A' and PEP inteins.
		Splicing of Mja Rpol A' and PEP inteins in MEIEP.
		The effect of the intein penultimate residue on protein splicing of the Mja Rpol A' intein in the MEIEP fusion protein.
	www.jbc.org /cgi/content/full/275/27/20431 (3901 words)

	A DnaB intein in Rhodothermus marinus: Indication of recent intein homing across remotely related organisms -- Liu and ...
		An intein is a protein sequence embedded in-frame within a precursor protein sequence and excised during maturation (1,
		inteins and are known to be critical for the chemistry of protein
		are significantly conserved among known inteins (2, 5).
	www.pnas.org /cgi/content/full/94/15/7851 (3213 words)

	Modulation of Protein Splicing of the Saccharomyces cerevisiae Vacuolar Membrane ATPase Intein -- Chong et al. 273 ... (Site not responding. Last check: 2007-10-21)
		The rapid splicing of the Sce VMA intein
		The protein splicing pathway of the Sce VMA intein (5) is indicated by the bold arrows.
		of an intein is responsible for the intein-mediated gene mobility
	www.jbc.org /cgi/content/full/273/17/10567 (7692 words)

	Inteins - protein introns
		The intein protein-splicing C-terminal (C) domain is composed of the two adjacent motifs in the C-terminal 25-40 aa (including the conserved aa immediately C' to the intein).
		Functional inteins with no EN domain (minimal inteins), the relation of the protein splicing domain to other Hint domains and the presence of different EN domains in inteins all indicate that the primeval inteins had no EN domains.
		However, it is not clear in which cases this relation between the inteins is due to vertical transfer (the usual inheritance, from an organism to its progeny) or horizontal transfer (movement of DNA across species).
	bioinformatics.weizmann.ac.il /~pietro/inteins (1349 words)

	Henry Paulus
		Our aim is to elucidate the mechanism of catalysis of protein splicing by defining the catalytic center of the self-splicing intein, both in terms of the amino acid side chains involved and their arrangement in 3-dimensional space.
		As a first step in our investigation, we cloned the RecA intein between two affinity tags as artificial exteins and genetically dissected away the portions of the intein that are involved in its homing endonuclease function so as to generate a minimal protein splicing element.
		The excision of these inteins by protein splicing is required for the function of these proteins and the inhibition of protein splicing will therefore be lethal to this parasite.
	www.bbri.org /faculty/paulus/paulus.html (1024 words)

	INTEIN (Site not responding. Last check: 2007-10-21)
		An intein is a segment of a protein that is able to excise itself and rejoin the remaining portions with a peptide bond.
		The segment of the gene that encodes the intein is usually given the same name as the intein, but to avoid confusion, the name of the intein proper is usually capitalized, whereas the name of the corresponding gene segment is italicized.
		It is licensed under the GNU free documentation license.
	www.yotor.org /wiki/en/in/Intein.htm (446 words)

	Algal Viruses with Distinct Intraspecies Host Specificities Include Identical Intein Elements -- Nagasaki et al. 71 ...
		intein; (iv) assess the implications of the diversity of this
		The intein region of HaV01 is shown as a fl square, and its amino acid sequence is shown in Fig.
		Inteins and introns of viruses infecting eukaryotic algae.
	aem.asm.org /cgi/content/full/71/7/3599 (5821 words)

	Virology Journal \| Full text \| A new example of viral intein in Mimivirus
		After the recent discovery of an intein in Chilo iridescent virus [3], an insect-infecting NCLDV of Iridoviridae, this is the second report of an intein sequence in a eukaryote-infecting virus.
		The splicing is defined by the self-catalytic excision of an intervening sequence ("intein") from a precursor host protein where it is located, and the concomitant ligation of the flanking amino- and carboxy-terminal fragments ("exteins") of the precursor.
		A shift in the base compositions between intein and extein coding sequences is considered as indicating a recent acquisition of inteins [20].
	www.virologyj.com /content/2/1/8 (3213 words)

	Virology Journal \| Abstract \| A new example of viral intein in Mimivirus
		Inteins are "protein introns" that remove themselves from their host proteins through an autocatalytic protein-splicing.
		After their discovery, inteins have been quickly identified in all domains of life, but only once to date in the genome of a eukaryote-infecting virus.
		The intriguing association of an extremophilic archaeal-type intein with a mesophilic eukaryotic-like PolB in Mimivirus is consistent with the hypothesis that DNA viruses might have been the central reservoir of inteins throughout the course of evolution.
	www.virologyj.com /content/2/1/8/abstract (229 words)

	Information Systems for Biotechnology News Report
		Inteins are internal protein elements mediating post-translational protein splicing.
		Inteins can artificially be split into an N-terminal half (Int-n) that contains the A and B motifs and a C-terminal half (Int-c) that contains the F and G motifs.
		When the split intein fragments are fused with different protein or peptide precursors, they are able to conduct protein trans-splicing that assembles two separate precursor molecules into a mature hybrid protein.
	www.isb.vt.edu /news/2003/news03.may.html (6779 words)

	Specificities and Functions of the recA and pps1 Intein Genes of Mycobacterium tuberculosis and Application for ...
		be interrupted by an intein sequence at the RecA-a and Pps1-b
		The MtuPps1 intein gene is specific for the M.
		Tagged inteins were detected by Western blotting of the SDS-PAGE (10% polyacrylamide) gels used to separate proteins from soluble (S) and insoluble (I) fractions of MtuPps1 and MtuRecA extracts and immunologic detection by using anti-V5 antibody.
	jcm.asm.org /cgi/content/full/40/3/943 (4818 words)

	InBase, the Intein Database -- Perler 28 (1): 344 -- Nucleic Acids Research (Site not responding. Last check: 2007-10-21)
		Inteins are in-frame intervening sequences that disrupt the
		Larger inteins range in size from 360 to 608 amino acids (5).
		intein (by convention in the field, the prototype intein is
	nar.oxfordjournals.org /cgi/content/full/28/1/344 (1055 words)

	Expressed protein ligation: Method and applications -- David et al. 271 (4): 663 -- FEBS Journal (Site not responding. Last check: 2007-10-21)
		Numbering of the amino acids of a precursor protein is made in the following way: the intein's N-terminal amino acid (Cys, etc.) is numbered as 1 whereas the C-terminal amino acid of the N-terminal extein is numbered as -1 and the N-terminal residue of the C-terminal extein is numbered beginning with +1.
		EPL for the synthesis of the Cys segment.
		of the intein because of the absence of the Asp cyclization.
	content.febsjournal.org /cgi/content/full/271/4/663 (7764 words)

	Exploring intein structures in SPDBV (Site not responding. Last check: 2007-10-21)
		Most inteins are composed of two domains: one is responsible for protein splicing, and the other has endonuclease activity.
		A few inteins have lost the endonuclease domain completely and retain only the self-splicing domain and activity.
		Open Saccharomyces cerevisiae intein that is bound to its recognition DNA sequence.
	web.uconn.edu /gogarten/bioinf/intein_structure.html (405 words)

	IMPACT-CN System, E. coli, New England Biolabs (Site not responding. Last check: 2007-10-21)
		In the presence of thiols such as DTT, β-mercaptoethanol or cysteine, the intein undergoes specific self-cleavage which releases the target protein from the chitin-bound intein tag.
		The T7 Universal Primer and Intein Reverse Primer are used for sequencing a target gene cloned in the C-terminal fusion vectors (pTYB1 and pTYB2).
		The intein forward primer is complementary to the intein sequence 117-141 nucleotides upstream from the intein cleavage site and used for sequencing a target gene cloned in the N-terminal fusion vectors (pTYB11 and pTYB12).
	www.neb.com /nebecomm/products/productE6900.asp (1815 words)

	Bacteriophage-Based Genetic System for Selection of Nonsplicing Inteins -- Cann et al. 70 (5): 3158 -- Applied and ...
		intein variants were recently published (1, 3, 11).
		Intein amino acid sequences are indicated above the precursor, and DNA polymerase sequences (exteins) are indicated below.
		Specificities and functions of the recA and pps1 intein genes of Mycobacterium tuberculosis and application for diagnosis of tuberculosis.
	aem.asm.org /cgi/content/full/70/5/3158 (1747 words)

	Lightning strikes twice: Intron-intein coincidence -- Derbyshire and Belfort 95 (4): 1356 -- Proceedings of the ... (Site not responding. Last check: 2007-10-21)
		The now familiar group I introns self-splice at the RNA level (3), whereas inteins, which are in-frame protein fusions,
		Approximately 70% of known inteins are confined to such functions.
		of the intron or intein to splice and therefore to preserve expression
	intl.pnas.org /cgi/content/full/95/4/1356 (1743 words)

	Synthetic Two-piece and Three-piece Split Inteins for Protein trans-Splicing -- Sun et al. 279 (34): 35281 -- Journal ... (Site not responding. Last check: 2007-10-21)
		An intein is a protein-intervening sequence that catalyzes a
		the latter conferring genetic mobility to the intein.
		part of the intein were 11 and 49 aa long, respectively.
	www.jbc.org /cgi/content/full/279/34/35281 (3274 words)

	Intein-mediated purification of cytotoxic endonuclease I-TevI by insertional inactivation and pH-controllable splicing ... (Site not responding. Last check: 2007-10-21)
		Inteins are the protein analogs of introns that exist as in-frame
		of the Mtu RecA intein was favored at pH <6.5 (10).
		Mills,K.V., Lew,B.W., Jiang,S. and Paulus,H. (1998) Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA intein.
	nar.oxfordjournals.org /cgi/content/full/30/22/4864 (4624 words)

	A second-generation system for unbiased reading frame selection -- Gerth et al. 17 (7): 595 -- Protein Engineering ...
		(D and E) pInSALect: the introduction of intein sequences (yellow) flanking the gene of interest facilitates the post-translational self-excision of the region linking the head and tail reporters.
		The gene encoding the VMA intein (GenBank No. AB093499) was
		of the parental intein sequence with the linker His–Met–Gly–Gly–Thr–Ser.
	peds.oxfordjournals.org /cgi/content/full/17/7/595 (4005 words)

	InBase, APSE1 dpol Intein (Site not responding. Last check: 2007-10-21)
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		First intein in DNA polymerase I. Inteins are now known in the catalytic subunits of all major families of DNA polymerases - Pol type A (this intein), type B (archaeal pol inteins), type C (Synechocystis polC split intein) and archaeal DP2 (also called type D, archaeal polC gene).
		Integration site is well conserved only in bacteriophage pol-I sequences.
	tools.neb.com /inbase/intein.php?name=APSE1+dpol (95 words)

	Phosphorylation of eIF4E attenuates its interaction with mRNA 5' cap analogs by electrostatic repulsion: ... (Site not responding. Last check: 2007-10-21)
		an intramolecular rearrangement, that is, excision of an intein
		milligram amounts of pure fusion protein with the active intein.
		Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element.
	www.rnajournal.org /cgi/content/full/9/1/52 (5381 words)

	Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility -- Chevalier and ... (Site not responding. Last check: 2007-10-21)
		an intron or intein) to a homologous allele that lacks the sequence
		Homing mechanisms of group I introns (left), inteins (center) and group II introns (right) in which the intervening sequence of gene X is duplicated in its cognate allele, gene X'.
		17 Perler,F.B., Olsen,G.J. and Adam,E. (1997) Compilation and analysis of intein sequences.
	nar.oxfordjournals.org /cgi/content/full/29/18/3757 (8356 words)

	InBase, Mex TrbC Intein
		Home > Technical Reference > InBase > Mex TrbC Intein
		Mex TrbC intein intein (including -1 and +1 extein residues)
		Obtained from Integrated Genomics IG_Jun02-Contig1227:3334-2 by S. Pietrokovski
	tools.neb.com /inbase/intein.php?name=Mex+TrbC (36 words)

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