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Topic: Isoenzyme

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EC number

Enzyme

Creatine Phosphokinase

Muscle

Phosphocreatine

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	Discovery Health :: Medical Tests :: ALP isoenzyme test
		Isoenzymes are usually only ordered if the total or basic ALP level is increased and the healthcare provider is not sure why.
		To measure levels of ALP isoenzymes, a blood sample is taken from a vein in the arm.
		Normal values for ALP isoenzymes vary based on the age and sex of the person and the type of test used.
	media.health.discovery.com /encyclopedias/illnesses.html?article=1028 (370 words)

	Notes from the Scientific Field
		Found type 1 isoenzyme in the nuclei of cells in all layers of the epidermis, in basal and glandular cells of sebaceous glands, in hair follicles (papilla, matrix cells, inner and outer root sheat, and arrector pili), and in eccrine and apocrine sweat glands.
		The type 2 isoenzyme is found in the skin and scalp from the birth to the age 2-3 years.
		Although type 1 isoenzyme is the dominant form of enzyme in adult scalp and the type 2 is dominant in prostate and genital tissue, in male pseudohermaphroditism patients’ virilization of the external genitalia occurs during puberty, although secondary sexual hair remains sparse and they seldom suffer from male-pattern baldness or acne.
	home.earthlink.net /~drfelman/notes.html (981 words)

	Determination of total creatine kinase or an isoenzyme with a multilayer analytical element - Patent 4713327
		Prior to or simultaneously with that contact, for an assay of an isoenzyme, the liquid sample is contacted with one or more antibodies which are capable of either preferentially reacting with or preferentially inhibiting the enzymatic activity of the isoenzymes not of interest, e.g.
		In this example, the B subunit of the CK-MB isoenzyme is ideally uneffected by the presence of the antibodies, and therefore, are free to react in any of a number of reaction schemes to produce a detectable change.
		Quantification of total creatine kinase or its isoenzyme in the practice of this preferred embodiment is achieved using oxygen as the electron acceptor and a colorimetric indicator composition which comprises: (1) a substance having peroxidative activity, and (2) a chromogen.
	www.freepatentsonline.com /4713327.html (6690 words)

	New sandwich ELISA for human urinary N-acetyl-{beta}-D-glucosaminidase isoenzyme B as a useful clinical test -- Numata ...
		isoenzyme B with the pair of Hex 31 and Hex 32.
		with NAG isoenzyme A was examined from pH 5.2 to 8.0 in the
		of NAG isoenzyme B was increased roughly in parallel with the
	www.clinchem.org /cgi/content/full/43/4/569 (2633 words)

	Hairloss-reversible.com: Isoenzyme
		Isoenzyme is an important word now especially since Glaxo may have their drug dutasteride on the market soon.
		Enzymes that are chemically distinct but act to catalyze the same reaction are called isoenzymes.
		The reaction that these isoenzymes catalyze is the conversion of testosterone into DHT.
	www.hairloss-reversible.com /discus/messages/4/17.html?994346815 (217 words)

	Lactate Dehydrogenase (LD) Isoenzymes (Site not responding. Last check: 2007-10-14)
		Such LD macroenzymes are characterized by abnormal position of isoenzyme bands, broadening or abnormal motility of a band and otherwise unexplained increase of total serum LD.
		The efficiency of lactate dehydrogenase isoenzyme determination for the diagnosis of acute myocardial infarction.
		Patterns of lactate dehydrogenase isoenzymes in serum of patients with acute pulmonary edema.
	www.labcorp.com /datasets/labcorp/html/chapter/mono/nf10003790.htm (1652 words)

	Alkaline Phosphatase Activity as a Clinical Chemistry Diagnostic Aid
		The isoenzymes of ALP that are of clinical use in veterinary medicine include the hepatic, corticosteroid-induced, bone, intestinal, and placental forms.
		This induced isoenzyme was found to be located in the area of the hepatocyte membrane that constitutes the bile canaliculi.
		The steroid and intestinal isoenzymes of ALP are resistant to levamisole inhibition, while all the other isoenzymes (hepatic, bone, placental) are susceptible to levamisole inhibition.
	www.vet.uga.edu /vpp/CLERK/raymond (1298 words)

	American Psychiatric Association (Site not responding. Last check: 2007-10-14)
		For combination antiretroviral therapy that includes protease inhibitors and nonnucleoside reverse transcriptase inhibitors, an overarching concern in terms of coadministration with psychotropic agents is not only the possibility of plasma levels of psychotropic medications outside the targeted therapeutic concentration range but also the possible reduction of antiretroviral levels to the point of risking their effectiveness.
		Benzodiazepines are metabolized primarily by the cytochrome P450 isoenzyme 3A; however, oxazepam, lorazepam, and temazepam are metabolized by direct conjugation via glucuronyl transferase, resulting in shorter elimination half-lives and less likelihood of metabolite accumulation and associated side effects.
		Because opioids are metabolized primarily via the cytochrome P450 isoenzymes 2D6 and 3A, they should be used cautiously in conjunction with antiretroviral agents that are likely to increase their plasma levels.
	www.psych.org /psych_pract/treatg/pg/hivaids_revisebook_6.cfm (13259 words)

	Alkaline Phosphatase (Site not responding. Last check: 2007-10-14)
		Bone - this isoenzyme is produced by osteoblasts and increases in serum in association with osteoblastic activity (young animals, certain bone disorders).
		Isoenzyme measurement is most commonly applied to canine samples to distinguish LAP and CAP isoenzymes in cases with increased total activity of uncertain cause.
		Differentiation of isoenzymes can be accomplished by electrophoretic (affinity agarose electrophoresis, cellulose acetate electrophoresis or isoelectric focusing on agarose) or differential inhibition methods using levamisole (inhibits LAP and BAP especially), heat (inactivates LAP and BAP) and wheat germ lectin (precipitates BAP and CAP) methods.
	www.diaglab.vet.cornell.edu /clinpath/modules/chem/alkphos.htm (866 words)

	Myocardial Markers
		The predominant LDH isoenzyme in liver and skeletal muscle is M4 (LDH-5) and the predominant cardiac isoenzyme is H4 (LDH-1).
		The CK isoenzymes are resolved by electrophoresis but many hospital laboratories conduct CK isoenzyme analysis by a simple procedure involving the use of an antibody against the M subunit which inhibits M activity while the activity of the B subunit is retained.
		Although the LDH isoenzyme pattern is indistinguishable from muscle disease or injury, the absence of at least a trace of CK-MB isoenzyme is inconsistent with the muscle CPK isoenzyme distribution as is the apparently normal total activity.
	home.comcast.net /~theWebDoc/Lectures/Fall/CardEnz/cardenz.htm (1761 words)

	Copper Induction of Laccase Isoenzymes in the Ligninolytic Fungus Pleurotus ostreatus -- Palmieri et al. 66 (3): 920 -- ...
		Copper Induction of Laccase Isoenzymes in the Ligninolytic Fungus Pleurotus ostreatus
		Zymogram of laccase isoenzymes in copper-supplemented cultures in the absence (lanes A) and in the presence (lanes B) of protease inhibitors.
		Laccases are produced as a number of isoenzymes that are encoded by gene families in several fungal species.
	aem.asm.org /cgi/content/full/66/3/920 (3658 words)

	SPIFE Alkaline Phosphatase Isoenzyme (via CobWeb/3.1 planetlab2.cs.virginia.edu) (Site not responding. Last check: 2007-10-14)
		Alkaline phosphatase (ALP) isoenzymes found in human serum originate from several sources with the greatest activity found in bone, liver, intestine, and the placenta.
		The isoenzymes differ in their physicochemical and electrophoretic properties, allowing identification.
		This control is for use as a qualitative and/or quantitative control to aid in the identification of alkaline phosphatase isoenzymes by agarose gel electrophoresis.
	www.helena.com.cob-web.org:8888 /catalog/spifealkphos.htm (234 words)

	TITAN GEL LD Isoenzymes
		Electrophoresis, the method of choice for LD isoenzyme analysis, provides quantitation of all five isoenzymes in one test procedure.
		Electrophoresis accurately quantitates the LD1 and LD2 isoenzymes, as well as provides meaningful information on potential complications and secondary disease states, particularly pulmonary infarction and hepatic congestion.
		Prepared with murine tissue extract and human serum, control is for quantitative and/or qualitative use in conventional agarose electrophoresis of CK and LD isoenzymes (3 CK and 5 LD isoenzymes present).
	www.helena.com /catalog/tgldisoenzyme.htm (169 words)

	AEGiS-05IAC: Serum LDH isoenzyme activity in HIV patients with respiratory disease.
		OBJECTIVE: To characterize the serum LDH isoenzyme pattern in HIV patients with elevated LDH and bronchoscopically diagnosed PCP or non-PCP respiratory pathogens.
		In non-PCP respiratory illness isoenzymes 3, 4, 5 are elevated.
		Further study is needed to confirm if isoenzyme patterns, in particular isoenzyme 2 and 3, are more predictive of PCP than total LDH in the HIV population.
	www.aegis.com /conferences/iac/1989/B537.html (307 words)

	JAMA -- Abstract: Peripheral source of MB band of creatine kinase in alcoholic rhabdomyolysis. Nonspecificity of MB ...
		Nonspecificity of MB isoenzyme for myocardial injury in undiluted serum samples, August 8, 1980, Siegel and Dawson 244 (6): 580
		The MG isoenzyme of creatine kinase (CK MB) was strongly positive in the
		MB isoenzyme may be detectable in serum from peripheral sources in the
	jama.ama-assn.org /cgi/content/abstract/244/6/580 (142 words)

	Protein Kinase C Isoenzyme Patterns Characteristically Modulated in Early Prostate Cancer -- Cornford et al. 154 (1): ...
		Similarly, foci of adjacent in situ neoplastic epithelial cells are negative, thus demonstrating the loss of this isoenzyme in early prostatic neoplasia.
		Stromal expression of this isoenzyme is not identified in these invasive neoplasms.
		Clemens MJ, Trayner I, Meneya J: The role of protein kinase C isoenzymes in the regulation of cell proliferation and differentiation.
	ajp.amjpathol.org /cgi/content/full/154/1/137 (3947 words)

	ALP (alkaline phosphatase) isoenzyme (Test)... Fort Lauderdale, Florida
		A blood test to measure the amounts of different forms (isoenzymes) of the enzymealkaline phosphatase.
		During some growth spurts, levels can be as high as 500 IU/L. Usually children are not measured because of the potential for such high amounts, so the abnormal results refer to adults.
		The isoenzymes can reveal whether the increase is because of "bone" ALP or "liver" ALP.
	www.browardhealth.org /15608.cfm (758 words)

	MedlinePlus Medical Encyclopedia: ALP (alkaline phosphatase) isoenzyme
		Alkaline phosphatase is an enzyme in the blood, intestines, liver, and bone cells.
		Its chemical structure varies (called isoenzymes) depending on where it is produced.
		The isoenzymes can reveal whether the increase is in "bone" ALP or "liver" ALP.
	www.nlm.nih.gov /medlineplus/ency/article/003497.htm (787 words)

	Isoenzyme type 1 of 5alpha-reductase is abundantly transcribed in normal human genital skin fibroblasts and may play an ...
		Isoenzyme type 1 of 5alpha-reductase is abundantly transcribed in normal human genital skin fibroblasts and may play an important role in masculinization of 5alpha-reductase type 2 deficient males -- Thiele et al.
		Articles by Thiele, S. Articles by Hiort, O. Isoenzyme type 1 of 5alpha-reductase is abundantly transcribed in normal human genital skin fibroblasts and may play an important role in masculinization of 5alpha-reductase type 2 deficient males
		contrast to 5RII (12), we hypothesize that this isoenzyme is
	www.eje-online.org /cgi/content/full/152/6/875 (3425 words)

	Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruzi -- ...
		The prefix number of each strain is the isoenzyme type of ref. 3.
		The hybrid strains (isoenzyme types 39 and 43) are shown in shaded boxes.
		The most striking evidence of genetic exchange is the inference that the nuclear genome of isoenzyme types 39 and 43 is hybrid.
	www.pnas.org /cgi/content/full/98/13/7396 (4235 words)

	Mannose Phosphate Isomerase Isoenzymes in Plutella xylostella Support Common Genetic Bases of Resistance to Bacillus ...
		A genetic analysis with isoenzyme loci was performed on the YHD2 strain of H.
		be indicative of linkage of the locus for this isoenzyme to the
		No significant differences in isoenzyme frequencies (Table 2) were detected between the LAB-V and the PHI populations or
	aem.asm.org /cgi/content/full/67/2/979 (1399 words)

	Expression and Regulation of a Silent Operon, hyf, Coding for Hydrogenase 4 Isoenzyme in Escherichia coli -- Self et ...
		Isolation and characterisation of a soluble active fragment of hydrogenase isoenzyme 2 from the membranes of anaerobically grown Escherichia coli.
		Molecular characterization of an operon (hyp) necessary for the activity of the three hydrogenase isoenzymes in Escherichia coli.
		Purification and properties of membrane-bound hydrogenase isoenzyme 1 from anaerobically grown Escherichia coli K12.
	jb.asm.org /cgi/content/full/186/2/580 (4525 words)

	Immunohistochemical Demonstration of Carbonic Anhydrase Isoenzyme VI (CA VI) Expression in Rat Lower Airways and Lung ...
		Immunohistochemical Demonstration of Carbonic Anhydrase Isoenzyme VI (CA VI) Expression in Rat Lower Airways and Lung -- Leinonen et al.
		Kivela J, Parkkila S, Parkkila AK, Rajaniemi H (1999) A low concentration of carbonic anhydrase isoenzyme VI in whole saliva is associated with caries prevalence.
		Leinonen J, Kivelä J, Parkkila S, Parkkila A-K, Rajaniemi H (1999) Salivary carbonic anhydrase isoenzyme VI is located in the human enamel pellicle.
	www.jhc.org /cgi/content/full/52/8/1107 (2909 words)

	High Lactate Dehydrogenase Isoenzyme 1 in a Patient with Malignant Germ Cell Tumor Is Attributable to Aberrant ...
		High Lactate Dehydrogenase Isoenzyme 1 in a Patient with Malignant Germ Cell Tumor Is Attributable to Aberrant Methylation of the LDHA Gene -- Ishikawa et al.
		High Lactate Dehydrogenase Isoenzyme 1 in a Patient with Malignant Germ Cell Tumor Is Attributable to Aberrant Methylation of the LDHA Gene
		Lactate dehydrogenase (LD; EC 1.1.1.27) isoenzymes are formed
	www.clinchem.org /cgi/content/extract/50/10/1826 (331 words)

	Isoenzyme-specific Interaction of Muscle-type Creatine Kinase with the Sarcomeric M-Line Is Mediated by NH2-terminal ...
		The residues E45 and Q93, which are also highly conserved and might contribute to the binding in a minor way are shown in blue.
		Caravatti, M., Perriard, J.C., Eppenberger, H.M. Developmental regulation of creatine kinase isoenzymes in myogenic cell cultures from chicken.
		Wallimann, T., Wyss, M., Brdiczka, D., Nicolay, K., Eppenberger, H.M. Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands: the phosphocreatine circuit’ for cellular energy homeostasis.
	www.jcb.org /cgi/content/full/149/6/1225 (6347 words)

	Measurement of Tartrate-Resistant Acid Phosphatase and the Brain Isoenzyme of Creatine Kinase Accurately Diagnoses Type ...
		TRAP is an isoenzyme of the nonspecific acid phosphatases that
		Walters LM, Schneider GB 1988 Tartrate-resistant acid phosphatase activity in tibial osteoclasts and cells elicited by ectopic bone and suture implants in normal and osteopetrotic rats.
		Lederer WH, Gerstbrein HL 1976 Creatine kinase isoenzyme BB activity in serum of a patient with gastric cancer.
	jcem.endojournals.org /cgi/content/full/87/5/2212 (3951 words)

	Lactate dehydrogenase isoenzyme 1 (S-LD-1) determinations...
		Lactate dehydrogenase isoenzyme 1 (S-LD-1) determinations in relation to stage and histology of testicular germ cell tumors
		to investigate different measurements of the five normal S-LD isoenzymes, in relation to the stage of the patients with testicular germ cell tumors, especially different measurements of S-LD isoenzyme 1 (S-LD-1).
		KEY WORDS: lactate dehydrogenase isoenzymes, testicular neoplasms, electrophoresis, electrophoresis, lactate dehydrogenase, human chorionic gonadotropin, alpha fetoproteins,.
	www.cancerprev.org /Journal/Issues/22/101/11/2704 (427 words)

	Creatine Kinase MM Isoenzyme (CKMM)
		You are here: Home » Antibodies » Enzymes A-C » Creatine Kinase MM Isoenzyme (CKMM)
		Matched to C7910-08 (Creatine Kinase MB Isoenzyme (CKMB) Mab Mo xHu) as the capture antibody.
		This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
	www.usbio.net /Product.aspx?ProdId=376 (141 words)

	Lactate ... 5 LDH 5 Antibody Search - Biocompare
		Lactate Dehydrogenase Isoenzyme 5 (LDH 5) Antibodies Antibodies
		Sheep Anti-Lactate Dehydrogenase Isoenzyme 5 (LDH 5) Polyclonal Antibody, Unconjugated
		Enzyme or dye to which the antibody is conjugated; More Information
	biocompare.com /matrixsc/3194/2/6/3596/Lactate+...+5+(LDH+5).html (110 words)

	(WO/1990/005917) PYRUVATE-KINASE-ISOENZYME-TYPE-M2 (TUMOUR M2-PK) SPECIFIC ANTIBODIES, PROCESS FOR PRODUCING THEM AND ... (Site not responding. Last check: 2007-10-14)
		(EN) Pyruvate kinase (ATP : pyruvate 2-0-phosphotransferase, EC 2.7.1.40) isoenzyme type M2 (tumour M2-PK) can be determined qualitatively, quantitatively and locally by means of antibodies in blood, plasma, tissue cultures and tissue sections as well as in the animal and human body.
		The corresponding antisera are obtained using high-purity pyruvate kinase isoenzyme type M2 (tumour-M2-PK) or fractions thereof as immunogen.
		The antibodies can be used in ELISA test systems to diagnose cancer, and to determine the malignity of cells, as well as to localize tumours in the body and for cancer therapy.
	www.wipo.int /pctdb/en/wo.jsp?WO=1990/05917 (318 words)

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