E16 isoforms, fixed with 3% paraformaldehyde, permeabilized with 0.5% Triton X-100, and processed for immunofluorescence using a polyclonal anti-HA-epitope tag antibody as primary antibody and anti-rabbit IgG coupled to FITC as secondary antibody.
E16 isoforms, fixed with 3% paraformaldehyde, permeabilized with 0.5% Triton X-100, and processed for immunofluorescence using a monoclonal anti-c-myc-epitope tag antibody and a polyclonal anti-HA-epitope tag antibody as primary antibodies and anti-mouse IgG coupled to Texas red and rabbit IgG coupled to FITC as secondary antibodies.
isoform was strongly expressed in the nucleus, the plasma membrane, and apparent centrosomes.
Multiple fast-twitch skeletal muscle TnT (fTnT) isoforms are present in fast-twitch skeletal muscles, as detected by MAb 3E4 in breast and gastrocnemius muscles.
The fast-twitch skeletal muscle isoform of TnI (fTnI) was found in breast and gastrocnemius muscles, whereas slow-twitch skeletal muscle isoform TnI (sTnI) was expressed in trapezius and cardiac isoform TnI (cTnI) was expressed in heart.
The developmental acidic-to-basic TnT isoform expression switch is found in avian and mammalian cardiac and skeletal muscles.
Force-calcium relationship depends on myosin heavy chain and troponin isoforms in rat diaphragm muscle fibers -- Geiger ...(Site not responding. Last check: 2007-10-08)
expression of TnT and TnI isoforms (12, 29, 32, 39) is
Western analysis was periodically performed to confirm the identification of MHC isoforms by migration patterns.
Similar differences in TnT isoforms have been found in other skeletal muscles when fast and slow fibers were compared.
Differential Subcellular Localization of Protein Phosphatase-1 alpha , gamma 1, and delta Isoforms during Both ...(Site not responding. Last check: 2007-10-08)
Isoforms of PP-1 are most divergent at their COOH termini.
A detailed comparison of isoform distributions in nuclei by optical sections of interphase cells is shown in Fig.
Inhibitor-2 functions like a chaperone to fold three expressed isoforms of mammalian protein phosphatase-1 into a conformation with the specificity and regulatory properties of the native enzyme.
of oestradiol induce the release of acidic isoforms (Padmanabhan
All COC were exposed to FSH isoform for 5 min after which some COC were transferred to control medium, whereas the remaining continued with FSH exposure.
Open symbols = data pooled from the less acidic isoform fraction (pI 6.43–5.69; one experiment) and the mid-acidic isoform fraction (pI 5.62–4.96; two experiments) (open squares: continued presence of FSH and open circles: removed from FSH isoform containing media after 5 min).
Expression of dominant-negative and mutant isoforms of the antileukemic transcription factor Ikaros in infant acute lymphoblastic leukemia -- Sun et al.
Expression of dominant-negative and mutant isoforms of the antileukemic transcription factor Ikaros in infant acute lymphoblastic leukemia
(A) Schematic representation of Ikaros isoforms 1-8 with specific composition of domains encoded by exons (E) 1-7 and the PCR primers.
Inclusion of exon 12A, as occurs with two additional isoforms expressed in the majority of NBs, is typically demonstrated in tissues of neural origin (isoforms containing exons 12A-12D, which were infrequently demonstrated in NBs, are also referred to as amphiphysin II).
isoform divided by the sum of the intensities of all present
BIN1 isoforms were detected in all 56 of the NBs assayed.
The IC calculated for each isoform is indicated at the right of the curves.
Data are expressed as fusion or infection activity and represent the following calculations: OD observed in the presence of CX3CR1 isoforms - OD observed in the presence of pcDNA3/binding observed in presence of CX3CR1 isoforms - binding observed in presence of pcDNA3.
These novel isoforms are potent in vitro HIV coreceptors.
With nine different isoforms of membrane-associated adenylyl cyclases (ACs) and one isoform of soluble AC, there is much to learn and even more to understand regarding the expression of tissue-specific AC isoforms.
Knowing how to tailor AC activity, or how to exploit the activity of one isoform over another in a given tissue, may give rise to therapeutic agents that can inhibit AC-dependent disease states or, at least, lessen their severity.
Hanoune, J. and Defer, N. Regulation and role of adenylyl cyclase isoforms.
Equal amounts (5 ng) of ectopically expressed and TALON column-purified fractions of either vector alone (lane 1) or the isoforms of TFII-I (lanes 2-5) were compared by Western blotting with an antibody (anti-TFII-I) that recognizes all the four isoforms (left panel).
The same amount of protein used for DNA binding in EMSA was analyzed by Western blot and probed with an anti-TFII-I antibody.
It is possible that a small fraction of isoforms can exist as heteromeric complex (with each other) in the cytoplasm.
Nuclear Import and Export Functions in the Different Isoforms of the AUF1/Heterogeneous Nuclear Ribonucleoprotein ...(Site not responding. Last check: 2007-10-08)
Nuclear Import and Export Functions in the Different Isoforms of the AUF1/Heterogeneous Nuclear Ribonucleoprotein Protein Family -- Sarkar et al.
A, schematic representation of the four AUF1/hnRNP D proteinisoforms showing RNA-binding domains (RBD) 1 and 2, exons 2 and 7, and the common CTD, which is interrupted by exon 7 in the p42 and p45 isoforms.
Immunoblots were probed with antibodies GFP for interacting GFP-p37 AUF1 or FLAG epitope to determine the level of immunoprecipitated AUF1 isoforms.
Two Thromboxane A2 Receptor Isoforms in Human Platelets . Opposite Coupling to Adenylyl Cyclase with Different ...(Site not responding. Last check: 2007-10-08)
We report that both isoforms are present in human platelets.
Production of multiple isoforms by alternative splicing has been reported in G protein-coupled receptor family (33).
Ichikawa (1993) Two isoforms of the EP receptor with different carboxyl-terminal domains.
It is now possible to submit all splice isoforms annotated in one Swiss-Prot entry to a multiple alignment, or to retrieve the sequences of all these isoforms, e.g.
Sequences of alternatively spliced isoforms of the same protein are documented in the feature table of that protein sequence record.
The additional isoform entries have been added to the SWISS- PROT/TrEMBL databases underlying the BLAST server at SIB/CSCS Switzerland, and ScanProsite.
Proceedings of the First Annual Meeting of the Kyoto Cornea Club, Kyoto, Japan, December 1-2, 1995.
Zoukhri, D., Hodges, R.R., Sergheraert, C., Toker, A., and Dartt, D.A.: Lacrimal gland PKC isoforms are differentially involved in agonist-induced protein secretion.
Zoukhri, D., Hodges, R.R., Willert, S., and Dartt, D.A.: Immunolocalization of lacrimal gland PKC isoforms.
In addition to viral proteins and antigenic peptides, a number of regulatory cellular proteins have non-AUG initiation codons.
For example, c-Myc has two distinct isoforms, the longer of which is initiated with CUG and may inhibit proliferation, as it is absent in a number of tumor-derived cell lines.
Intriguingly, synthesis of the CUG-initiated form increases when cells reach high density, specifically when methionine is limiting (Hann et al.
