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Topic: Isothermal titration calorimeter


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  CSC - Calorimetry Sciences Corp. - calorimeter - microcalorimeter, nanocalorimer   (Site not responding. Last check: 2007-10-28)
The Nano Isothermal Titration Calorimeter series III (N-ITC) is designed specifically for the study of biopolymer ligand interactions.
The Multi-Cell Differential Scanning Calorimeter (MC-DSC) is designed for the study of biopolymer stability in dilute solution and has sufficient sensitivity to study the unfolding of most proteins at concentrations in the 1 mg/ml range.
Equipped with removable cells, the ITC can be used to study interactions of solutes with immobilized enzymes, tissue samples, or other solid materials in suspension.
www.calscorp.com /Calorimeters.html   (624 words)

  
 Titration - Wikipedia, the free encyclopedia
Titrations are often recorded on titration curves, whose compositions are generally identical: the independent variable is the volume of the titrant, while the dependent variable is the pH of the solution (which changes depending on the composition of the two solutions).
As applied to biodiesel, titration is the act of determining the acidity of a sample of WVO by the dropwise addition of a known base to the sample while testing with pH paper for the desired neutral pH=7 reading.
Redox titration is a type of titration based on a redox reaction between the analyte and titrant.
en.wikipedia.org /wiki/Titration   (1018 words)

  
 Titration -- Facts, Info, and Encyclopedia article   (Site not responding. Last check: 2007-10-28)
Using a calibrated (Measuring instrument consisting of a graduated glass tube with a tap at the bottom; used for titration) burette to add the titrant, it is possible to determine the exact amount that has been consumed when the endpoint is reached.
The endpoint of a titration is when the pH of the reactant is just about equal to 7, and when the reactant stops reverting back to it’s original color.
Although the vast majority of titrations are carried out in aqueous solution, other solvents such as (Click link for more info and facts about glacial acetic acid) glacial acetic acid, are used for special purposes.
www.absoluteastronomy.com /encyclopedia/t/ti/titration.htm   (734 words)

  
 Calorimeter - Wikipedia, the free encyclopedia
The word calorimeter is derived from the Latin word calor, meaning heat.
Any microcalorimeter must have a low-heat-capacity mass to absorb incident X-ray photons, a weak link to a low-temperature heat sink which provides the thermal isolation needed for a temperature rise to occur, and a thermometer to measure change in temperature.
In particle physics, a calorimeter is a component of a detector that measures the energy of entering particles.
en.wikipedia.org /wiki/Calorimeter   (836 words)

  
 [No title]   (Site not responding. Last check: 2007-10-28)
The calorimeter of claim 3, wherein the pressure controller further comprises a control valve, the valve allowing either a first pressure source to apply a first pressure to the pressure chamber or a second pressure source to apply a second pressure to the pressure chamber.
The calorimeter of claim 9, wherein the reference cell also comprises a vessel shaped to contain a liquid, and the sample cell vessel and the reference cell vessel are substantially identical in mass and volume.
The calorimeter of claim 18, wherein the information sufficient to determine temperature differentials is selected from the group consisting of actual temperature differentials between the sample and reference cells, and differential power applied to the sample cell versus the reference cell in order to maintain the sample and reference cells at substantially equal temperatures.
www.wipo.int /cgi-pct/guest/getbykey5?KEY=01/09597.010208&ELEMENT_SET=DECL   (8004 words)

  
 Calorimeter   (Site not responding. Last check: 2007-10-28)
This kind of calorimeter is a common component in a high-energy particle physics experimental apparatus.
Most particles enter the calorimeter and initiate a particle shower, much like a cosmic ray shower, and the particles' energy is deposited in the calorimeter, collected, and measured.
Typically, calorimeters are segmented transversely to provide information about the direction of the particle or particles, as well as the energy deposited, and longitudinal segmentation can provide information about the identity of the particle based on the shape of the shower as it develops.
www.usedaudiparts.com /search.php?title=Calorimeter   (966 words)

  
 Biophysical Journal: Temperature Dependence of the Binding of Endotoxins to the Polycationic Peptides Polymyxin B and ...
At the beginning of the titration experiment (injection of 3 µm every 5 min) there is a strong endothermic reaction that decreases after the 12th to 13th injection.
The temperature dependence of the ITC titration in the range 20-40°C is shown in Fig.
The ITC binding curves were analyzed applying common laws of thermodynamics for the calculation of TΔS and the Gibbs free energy ΔG (Jelesarov and Bosshard, 1999).
www.findarticles.com /p/articles/mi_qa3938/is_200503/ai_n13504005/pg_2   (1201 words)

  
 Products   (Site not responding. Last check: 2007-10-28)
When equipped with accessory flow adsorption (perfusion), flow mixing, titration, and vapor adsorption cells, the CSC IMC can be used to study such varied processes as: solute dissolution, solvent and/or solute adsorption at surfaces, binding equilibria, heats of mixing and/or dilution, and plant or animal cell metabolism.
The CSC Isothermal Titration Calorimeter (ITC) is designed specifically for the study of biopolymer ligand interactions.
The ITC can detect heat effects as small as 0.1 µcal allowing a titration to be done with as little as 1 nmole of biopolymer.
www.calorimetrysciences.com /Products/Products.htm   (977 words)

  
 IMB - IMB Facilities & Resources   (Site not responding. Last check: 2007-10-28)
Isothermal titration calorimetry (ITC) is used in thermodynamic analyses of macromolecular interactions.
The MicroCal VP-ITC is a highly sensitive calorimeter that allows for analysis of dilute solutions of macromolecules (typically in the micromolar range for proteins).
Jelesarov I. & Bosshard H.R. (1999) Isothermal Titration Calorimetry and Differential Scanning Calorimetry as Complementary Tools to Investigate the Energetics of Biomolecular Recognition, J. Mol.
www.sb.fsu.edu /imb/stures/itc.html   (288 words)

  
 [No title]   (Site not responding. Last check: 2007-10-28)
The instrument is an isothermal titration calorimeter (MicroCal).
Isothermal titration calorimetry directly measures the thermodynamics of protein-ligand interaction.
The systems currently under study are: protein- protein interactions in ferredoxin-linked chloroplast enzymes; cAMP receptor protein (CRP) interacting with cAMP and double stranded DNA; cold shock protein interacting with the specific single-stranded DNA sequences; interactions of sterol and sterol derivatives with (S)-adenosyl-L- methionine;24(25)-sterol methyl transferase (SMT); interactions of metallo--lactamases with -lactam antibiotics.
www.cs.utexas.edu /ftp/pub/inderjit/Data/Text/nsf/9604753   (110 words)

  
 Boston Biomedical Research Institute
With a Differential Scanning Calorimeter (DSC), we can measure changes in the heat capacity of a solution as it is heated.
This is typically used to determine the stability of a whole macromolecule or its parts.
An Isothermal Titration Calorimeter (ITC) measures the heat of interaction between molecules; from such measurements we can determine how tightly they interact with each other and what type of forces are involved.
www.bbri.org /corefac/calorimeters.html   (338 words)

  
 Isothermal Titration Calorimetry Instrument Information at the Biophysics Core Facility: The Program In Biomolecular ...
Isothermal Titration Calorimetry of Protein-Protein Interactions, Pierce et al., 1999, Methods, 19:213-221.
The oxidation of the DTT as it reacted with O2 in the buffer was exothermic and was causing the baseline instability.
Further on this point, DTT is not the reducing agent of choice for ITC.
biomol.uchsc.edu /researchFacilities/BiophysicsCore/itc.html   (311 words)

  
 calorimetry   (Site not responding. Last check: 2007-10-28)
We continue to develop a number of experiments using the isothermal titration microcalorimeter (ITC) obtained from MicroCal, Inc., Northampton, Massachusetts for the chemisty and biochemistry curriculum.
The purchase of the equipment was supported by The Camille and Henry Dreyfus Foundation through its Special Grant Program in the Chemical Sciences and the National Science Foundation through an Instrumentation and Laboratory Improvement Grant in the Division of Undergraduate Education.
Since the "heat signal" is an almost universal property of binding reactions, ITC can be used to monitor the binding of ligands to (macro)molecules as well as macromolecular interactions.
www.williams.edu:803 /Chemistry/lkaplan/calorimetry.html   (521 words)

  
 MBAL   (Site not responding. Last check: 2007-10-28)
The purpose in establishing the laboratory was to assemble the necessary equipment for state-of-the-art biophysical analyses of biological macromolecules.
The MicroCal VP-DSC is a high sensitivity differential scanning calorimeter suitable for determining the heat capacity function of trace aqueous solutions of biological macromolecules.
The addition of a titrator attachment allows for the evaluation of unfolding upon a continuous increase in denaturant concentration.
wine1.sb.fsu.edu /biophyslab/MBAL.htm   (443 words)

  
 Calorimetry - Setaram, calorimetry and thermal analysis
Reaction, isothermal and scanning calorimeter (ambient to 300°C) Using SETARAM's exclusive 3D-Sensor calorimetric sensor, the C80 offers in-situ calorimetric measurement possibilities on mixtures, solid or liquid interactions with liquid or gas.
Large-volume, High-Temperature DSC and (drop) calorimeter (ambient to 1600°C) 96 LINE is a line of modular high temperature (ambient to 2100°C) and large volume thermal analyzers (drop-calorimeter, calorimeter, DTA, DSC, TGA, TMA).
Differential Reaction Calorimeters (DRC) are used in laboratories working on the optimization of industrial processes and the screening of chemical reactions.
www.setaram.com /Calorimetry.htm   (403 words)

  
 calorimeter on Encyclopedia.com   (Site not responding. Last check: 2007-10-28)
Differential scanning calorimeters sensor provides increased sensitivity.(Product: intruments)
Calorimeter performs six to eight tests per hour.(PITTCON 2004 Exposition)(Advertisement)
Maxine Smith, a clinical dietitian with Akron City Hospital in Akron, Ohio, uses a MedGem Indirect Calorimeter to measure Chris Mettee's resting metabolic rate.
www.encyclopedia.com /html/X/X-calorimter.asp   (203 words)

  
 titration   (Site not responding. Last check: 2007-10-28)
Once the number of moles of reactant that have been neutralised has been determined then it is easy to calculate the concentration in moles per litre.
As applied to biodiesel, titration is the act of determining the acidity of a sample of by the dropwise addition of a known base to the sample while testing with pH paper for the desired neutral pH=7 reading.
By knowing how much base neutralizes an amount of WVO, we discern how much base to add to the entire.
www.yourencyclopedia.net /Titration.html   (539 words)

  
 Chemistry at Hamilton   (Site not responding. Last check: 2007-10-28)
However, recent azide titrations of met-myoglobin performed on an isothermal titration calorimeter (ITC) clearly demonstrate that azide binding gives off heat and is therefore an exothermic binding event.
We have used isothermal titration calorimetry (ITC) to characterize the thermodynamics of Ca(II) binding to tetracycline, oxy-tetracycline and chloro-tetracycline.
A recent report demonstrated that similar ITC measurements yielded exothermic peaks as Ca(II) was titrated into a tetracycline solution.
www.chem.hamilton.edu /research/2001/research_elgren2001.html   (1021 words)

  
 [No title]   (Site not responding. Last check: 2007-10-28)
The ITC allows researchers to study almost any reaction or binding interaction.
S, and n can easily be determined for most binding interactions in a single, relatively fast (approx 1 hour) titration experiment.
Since temperature changes are independent of many solution properties, thermometric titration is applicable to systems where other analytical techniques would have great difficulty (e.g.
www.instech.com.tw /WENDO/CSC2.HTM   (403 words)

  
 Thermodynamics of polyelectrolyte solutions   (Site not responding. Last check: 2007-10-28)
Vapor pressure osmometry and isothermal Titration Microcalorimetry are experimental methods to determine the thermodynamic properties of polymer solvent system.
Isothermal Titration Microcalorimetry gives the heat of dilution directly from which we can calculate activity of the solution.
The osmotic coefficient and activity of water in aqueous NaPSS solution are found out using Vapor pressure osmometry and Isothermal titration calorimeter at different temperatures.
etdindividuals.dlib.vt.edu:9090 /archive/00000035   (319 words)

  
 CBC Equipment
People from outside the Department wishing to use the facilities should contact Mrs Joanne Castle in the first instance.
Isothermal titration calorimetry (ITC) is a technique for measuring the thermodynamic parameters of a reaction or interaction in solution.
It gives the heat released (or absorbed) when two species are mixed, i.e.
www-leeper.ch.cam.ac.uk /cbc/equipment.html   (887 words)

  
 Biophysics Instrumentation Facility - Instruments   (Site not responding. Last check: 2007-10-28)
An Isothermal Titration Calorimeter (ITC) measures the heat evolved or absorbed in interactions between biological macromolecules and ligands.
A Differential Scanning Calorimeter (DSC) provides a direct measure of the heat absorbed in biopolymer conformational transitions.
These are the only techniques that allow direct determination of the enthalpy and heat capacity changes, and (for ITC) the binding constant and stoichiometry of the reaction of interest in a single experiment.
www.biochem.wisc.edu /bif/instruments.html   (1146 words)

  
 ELX.com.au (Australia) - Biocalorimetry - Applications of Calorimetry in the Biological Sciences 2E, John E. Ladbury ...
Calorimeters have now become commonplace in bioscience laboratories.
There are general chapters highlighting the usage of the isothermal titration calorimeter and the differential scanning calorimeter, more advanced chapters on specific applications and tutorials that cover the idiosyncrasies of experimental methods and data analysis.
Titration Calorimetry as a Tool to Determining Thermodyanmic and Kinetic Parameters of Enzymes (M.L. Bianconi).
www.elx.com.au /item/0470849681   (637 words)

  
 biocal   (Site not responding. Last check: 2007-10-28)
RE@L has installed a MicroCal isothermal titration calorimeter for thermodynamic studies of protein-protein, protein-ligand and protein-DNA interactions.
For certain applications, the instrument is housed in an anaerobic glove box to enable measurements under anaerobic conditions.
Work within REAL is sponsored by MicroCal, particularly in the area of anaerobic isothermal titration microcalorimetry.
www.le.ac.uk /biochem/real/biocalorimetry.htm   (116 words)

  
 Differential Scanning and Isothermal Titration Calorimetry   (Site not responding. Last check: 2007-10-28)
Currently, the Department maintains a Microcal MC-2 high resolution adiabatic differential scanning calorimeter (DSC) used for the thermodynamic analysis of proteins, lipids and biological membranes in dilute solution.
MC-2 is computer controlled for data collection, analysis and plotting using Microcal ORIGIN software.
An Omega adiabatic isothermal titration calorimeter (ITC) is available for the thermodynamic analysis of macromolecule-ligand interactions in solution.
www.bumc.bu.edu /Dept/Content.aspx?PageID=408&DepartmentID=102   (60 words)

  
 Calorimeter - Encyclopedia Glossary Meaning Explanation Calorimeter   (Site not responding. Last check: 2007-10-28)
Here you will find more informations about Calorimeter.
If you find this encyclopedia or its sister projects useful,
This time linearity requires good design and good (computerized) temperature control.
www.encyclopedia-glossary.com /en/Calorimeter.html   (839 words)

  
 Titration   (Site not responding. Last check: 2007-10-28)
Cr = Mr /Vr Biodiesel As applied to biodiesel, titration is the act of determining the acidity of a sample of WVO by the dropwise addition of a known base to the sample while testing with pH paper for the desired neutral pH=7 reading.
Variations The titration process can also be monitored by different means than visually * using the change of potential: potentiometry electrochemistry * using the change of conductivity of the solution: conductometry * using the heat of reaction in an isothermal titration calorimeter * using spectroscopy
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www.talkroot.com /library/Titration.shtml.htm   (650 words)

  
 Calorimetry of Surfactant Solutions. Measurement of the Enthalpy of Mixing of Tetraethylene Glycol Monooctyl Ether and ...   (Site not responding. Last check: 2007-10-28)
The enthalpy of the mixing of water and tetraethylene glycol monooctyl ether (C
) was measured precisely as a function of concentration of surfactant and temperature by using the high-accuracy isothermal titration calorimeter.
The differential enthalpies of solution of monomer and micelle and the enthalpy of micelle formation were evaluated by applying the thermodynamic relations to the enthalpy of mixing.
pubs.acs.org /cgi-bin/jtext?jpcbfk/101/i18/abs/jp963454k   (217 words)

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